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Citations for Promega :
451 - 500 of 6179 citations for Endothelin 1 ET 1 ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... All samples were brought to room temperature and mixed with a 1:1 volume of nGlow solution (Promega, #N1110). Samples (90% of total volume ...
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bioRxiv - Developmental Biology 2022Quote: HEK293T cells were simultaneously transfected with 1 μg pCS2Cachd1-eGFP or pCS2Cachd1_V1122D-eGFP and 1 μg KDEL-tRFP plasmid using Fugene 6 transfection reagent (Promega). eGFP/tRFP expression was confirmed 24 hours post transfection and the cells fixed at 42 hours post transfection and imaged on a spinning disk microscope (see below).
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bioRxiv - Molecular Biology 2019Quote: ... Cell pellets were lysed in 300µl of Lysis Buffer (50mM Tris-HCl pH 7.5, 150mM NaCl, 1% Triton® X-100, 0.1% deoxycholate, Protease Inhibitor and RNasin Plus [1:500, Promega]),diluted to a final concentration of ~1µg/µl with 100mM Tris-HCl pH 7.5 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Cell media was then replaced with a 1:1 mixture of fresh media and Nano-Glo Luciferase reagent (Promega) and luminescence was measured using a SpectroMax M5e (Molecular Devices) ...
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bioRxiv - Immunology 2021Quote: ... The media from infected cells was then removed and 100 μl of 1:1 diluted PBS:Bio-Glo (Promega, G7940) luciferase substrate was added to each well ...
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bioRxiv - Cancer Biology 2020Quote: ... the samples were diluted to 1 M Urea with 10mM EPPS pH 8.2 and incubated overnight with 1:50 LysC (Wako Chemicals) and 1:100 Sequencing grade trypsin (Promega). Digests were acidified using TFA and tryptic peptideswere purified by tC18 SepPak (50 mg ...
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bioRxiv - Microbiology 2021Quote: ... the cells were lysed with a 1:1 ratio of virus to Bright-Glo luciferase assay media (Promega, #E2610) and the contents were analyzed on a luminometer (LUMISTAR Omega ...
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bioRxiv - Microbiology 2022Quote: ... cells were lysed by adding 100 µl of 1:1 (v:v) mixture of phenol red-free DMEM and Bright-Glo substrate (Promega) and luminescence detected using a Glomax Navigator (Promega) ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 mM DTT) in presence of 1 mM Adenylyl-imidodiphosphate (AMP-PNP) and RNase inhibitor RNAsin (0.5 U, Promega) for 25 min at 4°C with head-over-end rotation ...
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bioRxiv - Immunology 2022Quote: ... caspase-1 activity was quantified in supernatants using a commercially available assay (Caspase-GLO 1 Assay; Promega, Madison, WI) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Media were removed and 100 μl of CTG reagent (CellTiter-Glo®, Promega GmbH, Germany; 1:1 with PBS) per well was added ...
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bioRxiv - Microbiology 2023Quote: ... CM was then harvested by centrifugation and active caspase-1 measured with the Caspase-Glo 1 Inflammasome Assay (Promega) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... Sample C was treated with 1% Triton X-100 (TX100) followed by treatment with 1μg mL-1 trypsin (Promega). For the detergent treatment ...
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bioRxiv - Bioengineering 2023Quote: ... The reaction was initiated by the addition of 1-250 nM acylated peptide (Vivitide) and a 1:10,000 dilution of LgBiT subunit of the NanoBiT luciferase (Promega) and read using an Envision plate reader (XCite 2105 ...
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bioRxiv - Genomics 2022Quote: ... Nuclei were resuspended in 200 μL resuspension buffer (1x PBS, 1% BSA and 0.2U μl/1 RNasin Plus RNase Inhibitor (Promega)) and transferred to a 1.5 mL Eppendorf tube tube ...
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bioRxiv - Neuroscience 2023Quote: ... and Dounce homogenized in 500 µL of ice-cold RNase-free buffer polysome buffer (50 mM Tris pH 7.5, 100 mM KCl, 12 mM MgCl2, 1% NP-40, 1 mM DTT, 200U/ml Promega RNasin ...
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bioRxiv - Developmental Biology 2023Quote: ... a minimum of 1 µg of RNA was combined with 1 µl of Oligo (dT)15 primer (Promega, C110B) and nuclease-free water to make up a total of 5 µl and incubated at 70°C and 4°C for 5 min each ...
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bioRxiv - Developmental Biology 2023Quote: ... A minimum of 1 µg of RNA was combined with 1 µl of Oligo (dT)15 primer (Promega, C110B) and nuclease-free water to make up a total of 5 µl and incubated at 70°C and 4°C for 5 min each ...
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bioRxiv - Molecular Biology 2023Quote: ... Two μL of reverse transcription buffer 5X were added and the samples were incubated at 20°C for 15 min before addition of 1.5 μL of dNTP mix (1 mM of dATP, dGTP, dCTP, dUTP) and 1 μL of AMV enzyme (PROMEGA). Reverse transcription was performed at 42°C for 45 min and synthesized cDNA was recovered by ethanol precipitation as described above ...
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bioRxiv - Molecular Biology 2023Quote: ... The secondary antibody was a horseradish peroxidase polyclonal goat anti-mouse (from 1:5,000 to 1:10,000, depending on the primary antibody; Promega, W4021). Proteins were detected with the ECL chemiluminescence reagent (GE Healthcare ...
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bioRxiv - Biophysics 2023Quote: ... we stained the cells with 1 ml of a 1:2,000 TMR Halo-ligand solution (Promega, Cat. No.: G8251) to fluorescently label the Halo-tagged proteins ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were incubated with the primary antibodies (1D4 1:1500, Anti-Calnexin Sigma c4731 1:600, Anti-LgBiT Promega N7100 1:100 ...
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Bacteria Are a Major Determinant of Orsay Virus Transmission and Infection in Caenorhabditis elegansbioRxiv - Microbiology 2024Quote: ... Orsay Virus RNA1 level was quantified by qPCR using 1 μl of 1/5 diluted cDNA (GoTaq Promega A6001) and run on QuantStudio 3 Real Time PCR system ...
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Bacteria Are a Major Determinant of Orsay Virus Transmission and Infection in Caenorhabditis elegansbioRxiv - Microbiology 2024Quote: ... Orsay virus RNA1 level was quantified by qPCR using 1 μl of 1/5 diluted cDNA (GoTaq Promega A6001) and run on QuantStudio 3 Real Time PCR system (RNA1 qPCR primers GW194 and GW195)23.
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bioRxiv - Pathology 2022Quote: ... A total of 1 µg of extracted RNA was transcribed into cDNA using GoScript Reverse Transcription Kit (Promega). Quantitative real-time PCR was performed using FastStart SYBR Green Master mix (Roche ...
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bioRxiv - Developmental Biology 2019Quote: ... HRP coupled secondary antibodies (1/2,000, sigma) were used and blots were developed using an ECL kit (Promega).
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bioRxiv - Cell Biology 2019Quote: DNA from DLD-1 and nucleoporins-targeted cells was extracted with Wizard® Genomic DNA Purification Kit (Promega). Clones were genotyped by PCR for homozygous insertion of tags with two sets of primers listed in Supplementary Table 5.
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bioRxiv - Cell Biology 2020Quote: DNA from DLD-1 and nucleoporins-targeted cells was extracted with Wizard® Genomic DNA Purification Kit (Promega). Clones were genotyped by PCR for homozygous insertion of tags with two sets of primers listed below.
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bioRxiv - Synthetic Biology 2020Quote: ... a 1 mL aliquot was taken for genomic isolation through the Maxwell kit (Promega, Cat# AS1490, Madison WI). Successful integration was verified through qPCR ...
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bioRxiv - Synthetic Biology 2020Quote: ... a 1 mL aliquot was taken for genomic isolation using the Maxwell kit (Promega, Cat# AS1490, Madison WI). Successful integration was verified using qPCR ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ATP release from THP-1 cells was determined by the ENLIGHTEN ATP Assay System Bioluminescence Detection Kit (Promega). Cells were grown in 6-well plates and starved overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... Luciferase gene expression in E0771 and RM-1 cells was validated using a luciferase activity assay kit (Promega). Luciferase-labeled E0771 and RM-1 cells were maintained in RPMI 1640 media supplemented with 10% FBS ...
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bioRxiv - Plant Biology 2023Quote: The substrate of the nano-luciferase diluted at 1:100 (v/v) (furimazine, Kit from Promega (ref 1110)) was spread on seedling that grew on Agar plates ...
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bioRxiv - Microbiology 2024Quote: ... Relative quantification of viral RNA was performed using the GoTaq® 1-Step RT-qPCR System kit (Promega). The mixture contained 5 μL of 2x Master Mix ...
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bioRxiv - Biochemistry 2024Quote: The amount of intracellular Glu was measured in 1×106 cells using the kit Glutamate-Glo (J7021; Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were then digested with 1 μg LysC (Wako) overnight at room temperature and then with 1 μg sequencing grade trypsin (Promega) for 6 hours at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: The pGL-AP-1 plasmid containing 6 consensus AP-1 binding sites was co-transfected with the pRL-CMV plasmid (Promega) into cells using TransIT 2020 transfection reagent (Mirus Bio ...
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bioRxiv - Cell Biology 2019Quote: ... The reaction was diluted 1:4 with 50 mM ammonium bicarbonate and incubated with 1 µg of Trypsin Gold (Promega) at 37°C for more than 18 h before desalting ...
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bioRxiv - Cell Biology 2021Quote: ... Anti-mouse HRP conjugate (W4021, 1:10,000) and Anti-Rabbit HRP Conjugate (W4011, 1:10,000) were purchased from Promega (Madison, WI). Anti-COPI antibody was a gift from Charles Barlowe (Dartmouth Univ ...
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bioRxiv - Cell Biology 2020Quote: ... HRP Conjugate (W4011, 1:5000), and anti-Mouse IgG (H+L), HRP Conjugate (W4021, 1:5000) were purchased from Promega.
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bioRxiv - Cell Biology 2021Quote: ... Trypsin digestion was carried out overnight at 37°C with 1:50-1:100 enzyme-protein ratio of sequencing grade-modified trypsin (Promega) in 50 mM NH4HCO3 pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... The samples were diluted with 225 μL of 57.6 mM NH4HCO3 containing 1 mM TCEP and the mixtures treated with 1 μg of sequencing grade modified trypsin (Promega), incubated at 37 °C overnight and then acidified by addition of formic acid to 0.1 % v/v and stored at −20 °C ...
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bioRxiv - Cancer Biology 2019Quote: The activity of the inhibitors of hPD-1/hPD-L1 immune checkpoint was examined using the hPD-1/hPD-L1 Blockade Bioassay (Promega), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... Cells for gene targeting were transfected with 500 ng of donor and gRNA plasmids in ratio 1:1 using ViaFect (Promega) transfection reagent according to the manufacturer’s instruction ...
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bioRxiv - Developmental Biology 2020Quote: ... the cells for the luciferase assay were exposed to 100 μL of a 1:1 dilution of DMEM and luciferase substrate (ONE-Glo Luciferase Assay System, Promega), and luminescence from each well measured in a GloMax-96 Microplate Luminometer (Promega) ...
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bioRxiv - Cell Biology 2020Quote: ... cells were seeded on 24 mm glass coverslips and transfected with 2 µg plasmid DNA (1:1.3:3 ratio LAMP1:ER:opto-kinesin) and Fugene6 transfection reagent (Promega 1:3) for 20-30 h prior to imaging ...
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bioRxiv - Cancer Biology 2019Quote: ... and Jurkat T cells overexpressing hPD-1 and carrying a luciferase reporter gene under the control of Nuclear Factor of Activated T-cells Response Element (NFAT-RE) (hPD-1 Effector Cells, hPD-1 ECs, Promega) were cultured in RPMI-1640 medium (Biowest ...
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bioRxiv - Immunology 2020Quote: ... pCAG-Flag or pCMV-HA-N expression vectors using standard molecular cloning methods as described in our previous publications.30–32 The IFN-β luciferase reporter plasmid pGL3-IFN-β-Luc vector was constructed in our previous study.33,34 The IFNλ1 luciferase reporter plasmid pGL3-IFNλ1-Luc was constructed by inserting the 1000-bp promoter region of human IFNλ1 (nucleotides −1000 to +1, with the translation start site set as 1) into pGL3-Basic (Promega, USA) according to methods outlined in previous studies.13,34 The ISG luciferase reporter plasmid pISRE-Luc vector was purchased from Clontech (USA) ...
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bioRxiv - Molecular Biology 2020Quote: ... and proteins were sequentially digested with LysC (1:50, w/w; FUJIFILM Wako) for 3 h at 37 °C and trypsin (50:1, w/w; Promega) overnight at 37 °C ...
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Intrarenal B cells integrate in situ innate and adaptive immunity in human renal allograft rejectionbioRxiv - Immunology 2020Quote: ... Trypsin digestion was carried out overnight at 37 °C with 1:50 - 1:100 enzyme-protein ratio of sequencing grade-modified trypsin (Promega) in 50 mM NH4HCO3 pH 7.5 ...