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Citations for Promega :
151 - 200 of 8329 citations for Cow T Cell Surface Glycoprotein CD3 Gamma Chain CD3G ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... cloned into the pGEM T-Easy vector (Promega; according to the manufacturer’s instruction ...
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bioRxiv - Epidemiology 2019Quote: ... and cloned into pGEM-T vector (Promega, USA). In total six 16S rDNA and two rpoB clones were sequenced and analysed using the BLASTN algorithm and finally aligned using Spiroplasma glossinidia data from NCBI as reference.
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bioRxiv - Molecular Biology 2020Quote: ... we ligated PCR amplicons to pGEM-T (Promega) vector system and confirmed that all picked clones encompassed TATA box deletion and guide6 reconstruction using Sanger sequencing.
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bioRxiv - Molecular Biology 2020Quote: ... and cloned in pGEM-T easy vector (Promega); Four to six positive clones were sequenced (MWG ...
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bioRxiv - Evolutionary Biology 2021Quote: ... cloned into pGEM-T Easy vectors (Promega, USA) and then transformed into competent Escherichia coli cells for amplification ...
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bioRxiv - Developmental Biology 2019Quote: ... PCR products were cloned into pGEM-T (Promega) and sequenced ...
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bioRxiv - Developmental Biology 2019Quote: ... and inserting it into pGEM-T Easy (Promega) by TA cloning ...
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bioRxiv - Cell Biology 2021Quote: ... Self-ligated empty pGEM-T vector (Promega, USA) was used to ensure equal amounts of transfected DNA ...
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bioRxiv - Cancer Biology 2021Quote: ... cloned into pGEM®-T Easy Vector (Promega), and then into the pSec-Tag2/hygroA vector (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... and TA cloned into pGEM-T Easy (Promega) then transformed into DH5α competent E ...
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bioRxiv - Microbiology 2020Quote: ... Splice2 was cloned into pGem-T Easy (Promega) and integrity of the DNA confirmed by sequencing ...
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bioRxiv - Microbiology 2022Quote: ... cloned into the pGEM-T easy plasmid (Promega), and subcloned into the pRKlacZ290 vector to generate transcriptional fusions to the promoterless lacZ gene ...
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bioRxiv - Microbiology 2023Quote: ... The pGEM-T Easy Vector (Promega, Madison, USA) and pMG36e vector (Addgene ...
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bioRxiv - Cell Biology 2023Quote: ... cloned in pGEM-T Easy empty vector (Promega) and sequenced ...
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bioRxiv - Microbiology 2023Quote: ... The amplicon cloned into pGEM T-EASY (Promega). An 804 bp portion of the hsdM1 coding sequence was deleted by inverse PCR with the inclusion of a unique BamHI site in each of the following primers ...
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bioRxiv - Molecular Biology 2023Quote: ... and cloned into pGEM-T Easy Vector (Promega). Positive clones were identified by the blue/white screening method and sequenced on ABI PRISM 3100 automated sequencer at Eurofins Genomics Europe Sequencing GmbH.
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bioRxiv - Molecular Biology 2023Quote: The pGEM®-T Easy Vector System (Promega) was used according to the manufacturer protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... PCR fragments were cloned in pGEM-T (Promega), and the inserts were sequenced by Sanger sequencing (University of California Berkeley DNA Sequencing Facility) ...
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bioRxiv - Microbiology 2024Quote: ... ligated into the linearized vector pGEM-T (Promega) and transformed via electroporation into E ...
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bioRxiv - Microbiology 2019Quote: ... quantified with Qubit dsDNA HS Assay kit and cloned using the pGEM®-T Easy Vector Systems (Promega, Madison, Wisconsin, US). Plasmids were isolated using the NucleoSpin Plasmid kit (Macherey-Nagel ...
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bioRxiv - Plant Biology 2019Quote: ... The PCR products were purified using a HiPure Gel Pure DNA Mini Kit (Magen, D2111) and cloned into the pGEM-T Easy Vector (Promega, A1360). For each sample ...
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bioRxiv - Plant Biology 2023Quote: ... 1 µl of duplex DNA was ligated into 25 ng of pBract214m-HvCas9-HSPT vector linearized with AarI using the ligation reagents and protocol described in pGEM®-T Easy Vector System kit (Promega). The ligation reaction was incubated overnight at 4°C then transformed into competent E ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were cultured in a 6-well plate and transfected with 10 µg of linearised plasmid expressing the SV40 Large T antigen using the FuGene Transfection Kit (Promega, #E2691) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Genomic DNA fragments generated by PCR were cloned into the pGEM-T Easy plasmid vector using the pGEM®-T Easy Vector System (Promega) following the manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: The prepared PCR fragments were inserted into the pGEM-T vector via T/A cloning following the manufacturer’s recommendations (Promega, Fitchburg, USA). Competent Escherichia coli were transfected with the ligation mix and plated on agar plates as described previously [13] ...
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bioRxiv - Systems Biology 2022Quote: Cell Titer Glo Luminescent Cell Viability Assay Kit (Promega) was used and subsequently performed after the BRET measurement ...
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bioRxiv - Biophysics 2021Quote: ... All DNA handles were made by polymerase chain reaction (PCR) using λ DNA cl857 Sam7 (Promega, D1501) as a template and a forward primer containing two digoxigenin labels at the 5’ end ...
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bioRxiv - Immunology 2023Quote: ... and the corresponding SARS-CoV-2 spike construct were co-transfected in HEK293-T cells using FuGENE 6 Transfection Reagent (Promega) in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Genomics 2019Quote: ... The A-tailed PCR products were then ligated to pGEM®-T Easy Vector using pGEM®-T Easy Vector Systems (Cat. A1360, Promega). Positive colonies were selected for Sanger sequencing ...
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bioRxiv - Microbiology 2020Quote: ... or the region between abaR and the abaI (for the abaI fusion) were amplified by PCR and ligated in pGEM®-T Easy using the pGEM®-T Easy Vector System (Promega). The resulting plasmids and the promoterless luxCDABE operon were digested with KpnI and BamHI and ligated in order to introduce the lux operon downstream of the predicted promoter of abaM or abaI ...
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bioRxiv - Microbiology 2019Quote: ... The membrane was then washed with TBS-T and incubated with horseradish peroxidase-conjugated anti-mouse IgG (diluted 1:1,000 in TBS-T) (Promega, Madison, WI, USA) for 1 h at 37°C ...
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bioRxiv - Plant Biology 2020Quote: ... the band of interest excised from the gel and cleaned using Macherey-Nagel™ NucleoSpin™ Gel and PCR Clean-up Kit and cloned into pGEM-T (Promega) for amplification before sequencing.
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bioRxiv - Microbiology 2019Quote: ... quantified with Qubit dsDNA HS Assay kit and then cloned using the pGEM®-T Easy Vector Systems (Promega, Madison, Wisconsin, US). Plasmids were isolated using the NucleoSpin Plasmid kit (Macherey-Nagel ...
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bioRxiv - Genetics 2019Quote: ... The longer band containing exon14b was then excised from the gel and purified using Zymoclean gel DNA recovery kit (Zymoresearch, Irvine, CA) and cloned using pGEM-T Easy Vector System (Promega, Madison, WI). The plasmid was used in a transformation into Subcloning Efficiency DH5α Competent Cells (Invitrogen ...
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bioRxiv - Genetics 2021Quote: ... using the pGEM-T Easy Vector Systems protocol (Promega) and an LB-ampicillin selective medium ...
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bioRxiv - Molecular Biology 2019Quote: ... Products were cloned into pGEM-T Easy vector (Promega). Bacterial colonies obtained after transformation were screened by colony PCR for the presence of inserts of appropriate size ...
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bioRxiv - Microbiology 2019Quote: ... and pGEM-T Easy vector (Promega, Madison, WI, USA) were amplified by PrimeSTAR® MAX DNA Polymerase (TaKaRa Bio ...
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bioRxiv - Microbiology 2019Quote: ... which was then ligated into pGEM-T vector (Promega), generating pGEM-T-Δslo-2kb ...
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bioRxiv - Genetics 2019Quote: ... purified and cloned into pGEM-T easy vector (Promega). Recombinant clones were then sent to Macrogen (South Korea ...
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bioRxiv - Microbiology 2019Quote: ... These amplicons were cloned into pGEM-T Easy (Promega), and transformed into E ...
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bioRxiv - Genetics 2020Quote: ... A DNA fragment containing 200 bp of upstream HO flanking region and 200 bp of the HO gene was synthesized by Life Technologies and cloned into pGEM®-T Easy (Promega Corp.) as a NotI fragment (pCB4386) ...
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bioRxiv - Physiology 2019Quote: ... cloned into pGEM-T vector (Promega, Madison, WI, USA) and nucleotide sequence was confirmed by Sanger sequencing (Center for Applied Genomics ...
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bioRxiv - Genetics 2021Quote: ... and ligated into pGEM®-T Easy vector (Promega) using 1:3 molar vector:insert ratio ...
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bioRxiv - Biochemistry 2020Quote: ... Amplicons were cloned into pGEM-T Easy vector (Promega) and sequenced.
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bioRxiv - Plant Biology 2020Quote: ... and cloned into the pGEM-T Easy Vector (Promega) using DNA Ligation Kit
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bioRxiv - Microbiology 2020Quote: ... using the pGEM®-T easy Vector System (Promega). Duplicate PCR products from the samples were pooled and purified with the Wizard® Gel and PCR Clean-Up System (Promega) ...
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bioRxiv - Plant Biology 2022Quote: ... sub-cloned into the pGEM T-Easy Vector (Promega), amplified with primers attB1 5’-TTACTCCATGTGTCAATACCAAAA-3’ and attB2 5’-GTCCATTTTAGTTCTCGAGTCGG-3 and introduced into the pDONR207 Gateway donor vector (Invitrogen) ...
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bioRxiv - Pathology 2022Quote: ... and cloned into a pGEM-T easy vector (Promega). Recombinant clones were then sent to Macrogen (South Korea ...
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bioRxiv - Genomics 2022Quote: ... .pGEM-T easy cloning vector (Promega, Madison, WI, USA) was used for cloning ...
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Cloning and In silico characterization of Heat shock factor (Hsf2) from Wheat (Triticum aestivum L.)bioRxiv - Molecular Biology 2020Quote: ... and cloned into pGEM-T simple vector (Promega, USA) in Escherichia coli strain M13 for sequencing.