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Citations for Promega :
51 - 100 of 8329 citations for Cow T Cell Surface Glycoprotein CD3 Gamma Chain CD3G ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: Total RNA was isolated from control (NTC) or DDX39B-depleted (Sh3 or Sh5) CD4+ T cells using ReliaPrep RNA Cell Miniprep System (Promega), and treated in-column with DNase I following the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2019Quote: ... and Jurkat T cells overexpressing hPD-1 and carrying a luciferase reporter gene under the control of Nuclear Factor of Activated T-cells Response Element (NFAT-RE) (hPD-1 Effector Cells, hPD-1 ECs, Promega) were cultured in RPMI-1640 medium (Biowest ...
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bioRxiv - Developmental Biology 2022Quote: ... and purified using a Nucleospin PCR clean-up kit and subcloned into p-GEM T-easy (Promega). Antisense RNA probes were synthesized using T3 ...
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bioRxiv - Cell Biology 2023Quote: ... PCR products were ligated into the pGEM®-T vector using a TA cloning kit (A3600, Promega), transformed into E ...
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bioRxiv - Cell Biology 2023Quote: ... PCR products were ligated into the pGEM®-T vector using a TA cloning kit (A3600, Promega), transformed into E ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and purified using a Nucleospin PCR clean-up kit and subcloned into p-GEM T-easy (Promega). Antisense RNA probes were synthesized using SP6 RNA polymerase and the DIG-labelling kit (Roche ...
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bioRxiv - Neuroscience 2020Quote: ... and inserting epitope tags via polymerase chain reaction with Pfu DNA polymerase (Promega). Primers for HA insertion into pIRES2- EGFP-FL ...
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bioRxiv - Zoology 2021Quote: ... Polymerase chain reactions (PCR) were carried out using GoTaq Green Master Mix (Promega) in the following conditions ...
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bioRxiv - Cancer Biology 2021Quote: ... Quantitative polymerase chain reaction (qPCR) was performed with the GoTaq qPCR Mastermix (Promega) and QuantStudio 5 Real Time PCR (Applied Biosystems) ...
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bioRxiv - Microbiology 2021Quote: ... Real time-polymerase chain reactions were performed with GoTaq qPCR Master Mix (Promega) according to the manufacturer’s instructions and completed on QuantStudio 6 Flex system (ABI) ...
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bioRxiv - Microbiology 2022Quote: Polymerase chain reaction was performed using the GoTaq PCR master mix (Promega, USA) as per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... and resulting PCR products were subcloned into a pGEM-T vector (Promega, pGEM-T Vector Systems) for subsequent Sanger sequencing of both alleles ...
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bioRxiv - Biochemistry 2022Quote: HEK293 T cells were co-transfected with the target receptor construct and GloSensor cAMP reporter (Promega) in DMEM supplemented with 10% FBS for overnight incubation ...
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bioRxiv - Immunology 2024Quote: ... Luciferase activity was measured in purified CD8 T cell lysates with the luciferase assay system (Promega). Purified CD8 cells were lysed using cell lysis buffer 1X ...
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bioRxiv - Immunology 2021Quote: ... or pGEM-T plasmid (Promega, UK), manufacturer’s instructions were followed ...
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bioRxiv - Developmental Biology 2021Quote: ... serial dilutions of pGEM-T (Promega) plasmids containing coding sequences of the target genes or internal control genes (rp49 for Drosophila melanogaster and AeRpL32 (AAEL003396 ...
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bioRxiv - Plant Biology 2020Quote: ... cloned in pGEM-t Easy (Promega), re-amplified by PCR with primers with attB tails and BP-cloned into pDONR207 (Invitrogen) ...
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bioRxiv - Neuroscience 2020Quote: The pGEM-T donor vector (Promega) was engineered by introducing a donor construct ...
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bioRxiv - Molecular Biology 2020Quote: ... subcloned via pGEM-T easy (Promega) and inserted upstream of a chloramphenicol resistance cassette in the plasmid pSF36 (pUC19+cm-FRT-HindIII ...
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bioRxiv - Microbiology 2019Quote: ... cloned into pGEM-T Easy (Promega), and then inserted using the NotI site ...
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bioRxiv - Microbiology 2020Quote: ... ligated to pGEM-T Easy (Promega), and transformed into E ...
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bioRxiv - Molecular Biology 2021Quote: ... or pGEM-T-Easy vector (Promega). For cloning into pGEM-T-Easy vector ...
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bioRxiv - Plant Biology 2022Quote: ... ligated into pGEM-T (Promega, USA), and checked by sequencing ...
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bioRxiv - Cell Biology 2023Quote: ... cloned in pGEM-T vector (Promega) and sequenced (Macrogen) ...
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bioRxiv - Microbiology 2023Quote: ... Plasmid pGEM®-T Easy (Promega) was used as a primary cloning vector ...
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bioRxiv - Cell Biology 2019Quote: ... ELISA data was normalized to viable cell number determined by MTT assay (Boehringer Ingelheim) or CellTiter-Blue (Promega). IFN levels were determined using a HEK293T IFN reporter cell line (clone 3C11 ...
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bioRxiv - Microbiology 2024Quote: ... or 4 μg of the pNL4.3-ΔEnv EGFP backbone36 (for viral entry assay in primary CD4+ T cells) into 293T cells using the FuGENE6 transfection reagent (Promega, USA). The cells were cultured at 37°C for 6 hours before the medium was completely replaced with fresh medium ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were inserted into the pGEM-T easy vector kit as described by the manufacturer (Promega, USA). Insert were then PCR-amplified and the resulting PCR products were sequenced.
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bioRxiv - Physiology 2022Quote: ... the PCR products were subjected to T/A cloning (pGEM®-T Vector Systems; Promega, Fitchburg, USA) with subsequent Sanger sequencing (Sanger et al ...
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bioRxiv - Plant Biology 2023Quote: ... The fragments were T-cloned using the pGEM®-T Easy Vector System I (cat A1360, Promega) and plated on X-gal/IPTG plates ...
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bioRxiv - Developmental Biology 2024Quote: ... and cloned into pGEM-T Easy vector using pGEM-T Easy Vector System I (Promega; CAT A1360). Vectors were transformed into Escherichia coli using the heat-shock method ...
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bioRxiv - Plant Biology 2021Quote: ... http://cshprotocols.cshlp.org/content/2009/2/pdb.prot5110.abstract) with trypsin (porcine, side chain protected; Promega). Briefly ...
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bioRxiv - Biophysics 2023Quote: ... followed by polymerase chain reaction (PCR) with GoTaq® G2 Master Mix (M7822, Promega) on Biometra TRIO Touch Thermocycler (207072X ...
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bioRxiv - Immunology 2019Quote: ... In vitro pmel-1 T cell-mediated cytotoxicity assay with CytoTox-ONE Homogeneous Membrane Integrity Assay (Promega). protocols.io dx.doi.org/10.17504/protocols.io.6j4hcqw
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bioRxiv - Cancer Biology 2023Quote: ... regions of mtDNA were cloned from donor cell lines into p-GEM-T Easy vector (Promega, A1360). Samples were then diluted to specific concentrations and analyzed to evaluate probe specificity.
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bioRxiv - Immunology 2020Quote: ... ExpiCHO cells then were incubated with Jurkat cells expressing FcγRIIIa receptor or FcγRIIa on their surface and stably transfected with NFAT-driven luciferase gene (Promega, Cat. Nr.: G9798 and G7018) at an effector to target ratio of 6:1 for FcγRIIIa and 5:1 for FcγRIIa ...
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bioRxiv - Microbiology 2022Quote: PCR amplicons were gel extracted using the QIAquick gel extraction kit and ligated into pGEM-T Easy plasmid (Promega). Vectors containing the PCR products were then cloned into chemically competent E ...
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bioRxiv - Microbiology 2019Quote: ... followed by cloning into pGEM-T (Promega) and sequencing.
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bioRxiv - Evolutionary Biology 2020Quote: ... or pGEM-T Easy Vector (A1360, Promega) (pax9 ...
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bioRxiv - Developmental Biology 2020Quote: ... cloned into pGEM-T Easy vector (Promega) and validated by sequencing ...
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Neurotransmission and neuromodulation systems in the learning and memory network of Octopus vulgarisbioRxiv - Neuroscience 2021Quote: ... ligated into p-GEM T vector (Promega) and linearized with the appropriate restriction endonucleases ...
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bioRxiv - Molecular Biology 2020Quote: ... cloning into PGEM T-easy vector (Promega) and transformation in E ...
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bioRxiv - Molecular Biology 2020Quote: ... then cloned into pGEM-T vector (Promega), to reveal the nucleotide sequence of each clone by Sanger sequence ...
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bioRxiv - Molecular Biology 2019Quote: ... inserted into pGEM T-Easy vector (Promega) and sequenced for determination of SHIP2 genotype.
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bioRxiv - Synthetic Biology 2020Quote: ... An adapted version of pGEM-T (Promega) was used to build the Step 1 crRNA and sgRNA cloning vector ...
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bioRxiv - Neuroscience 2019Quote: ... and ligated into pGEM-T (Promega protocol). Ligations were transformed into JM109 cells (Promega) ...
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bioRxiv - Genetics 2020Quote: ... ligated into pGEM-t-easy vector (Promega) and transformed into DH5α cells ...
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bioRxiv - Neuroscience 2021Quote: ... and cloned into pGEM-T vector (Promega), using the following primers ...
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bioRxiv - Cancer Biology 2020Quote: ... sub-cloned into pGEM-T Easy (Promega) and 4 or more clones per fragment were sequenced using standard Sanger sequencing ...
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bioRxiv - Microbiology 2021Quote: ... and ligated to pGEM-T Easy (Promega) according to the manufacturer’s instructions ...