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Citations for Promega :
251 - 300 of 5849 citations for Cow Eukaryotic Translation Initiation Factor 1 EIF1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... washed with PBS and inflammasome activation was assessed using qPCR or inflammasome caspase-1 activity was measured using Caspase-Glo® 1 inflammasome assay kit (G9951; Promega) according to manufacturer’s instructions.
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bioRxiv - Cancer Biology 2023Quote: ... viable cells were quantified in a FLUOstar OPTIMA ELISA reader (550/590 nm) about 2 h after CellTiter-Blue staining (Promega, Cat No. G8081). Mean values +/-standard deviations (SD ...
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bioRxiv - Immunology 2020Quote: ... and cDNA synthesized from 1 µg total RNA (High Capacity cDNA Reverse Transcription Kit, Promega). Quantitative RT-PCR was performed using FluoCycle II SYBR Master Mix (EuroClone ...
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bioRxiv - Neuroscience 2024Quote: ... using BRYT Green Dye contained in the GoTaq 1-Step RT-qPCR kit (Promega: A6020) in the CFX96 Touch Real-Time PCR Detection system (Bio-Rad) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Total DNA was isolated from 1 ml culture using the Wizard Genomic DNA Purification Kit (Promega). Concentration and quality of the extracted DNA was assessed using the NanoDrop™ (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... 1 µg of RNA was used to create cDNA with the ImpromII Reverse Transcriptase Kit (Promega) following the manufactures standard protocol including oligodT oligos and 4.8 M MgCl2 in each 20 µl reaction (Promega) ...
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bioRxiv - Developmental Biology 2022Quote: ... first-strand cDNA was synthesized using 1 µg total RNA with MMLV Reverse Transcription Kit (Promega) and poly-T primer ...
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bioRxiv - Microbiology 2021Quote: ... Viral RNA was quantified by real-time RT-qPCR (GoTaq 1 step RT-qPCR kit, Promega). Quantification was provided by serial dilutions of an appropriate T7-generated synthetic RNA standard ...
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bioRxiv - Microbiology 2022Quote: Viral RNA was quantified by real-time RT-qPCR (GoTaq 1 step RT-qPCR kit, Promega). Quantification was provided by serial dilutions of an appropriate T7-generated synthetic RNA standard ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative Realtime PCR (qRT-PCR) was performed using GoTaq® 1-Step RT-qPCR kit (Promega) with primers specific for PEX1 (RE7039-RE7040 ...
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bioRxiv - Microbiology 2023Quote: ... viral RNA was quantified by real-time RT-qPCR (GoTaq 1 step RT-qPCR kit, Promega). Quantification was provided by serial dilutions of an appropriate RNA standard ...
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bioRxiv - Zoology 2024Quote: One-step RT-qPCR was conducted using GoTaq 1-Step RT-qPCR System kit (Promega, France) in total reaction volume of 20 µl containing 2 µl of RNA extracts and 300 nM of the same forward and reverse primers as above ...
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bioRxiv - Microbiology 2023Quote: RT-qPCR was performed using the GoTaq 1-Step RT-qPCR kit (Promega Cat. No. A6020) using nifH and 16S rRNA (reference gene ...
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bioRxiv - Microbiology 2023Quote: ... Complimentary DNA was prepared from 1 µg of RNA using a Reverse Transcription Systems Kit (Promega). Quantitative PCR (qPCR ...
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bioRxiv - Microbiology 2024Quote: ... Viral RNA was quantified by real-time RT-qPCR (GoTaq 1 step RT-qPCR kit, Promega). Quantification was provided by serial dilutions of an appropriate T7-generated synthetic RNA standard ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Bacterial DNA was extracted from 1 ml of culture using the Wizard genomic DNA purification kit (Promega). Evolved population DNAs were sequenced at the GeT-PlaGe core facility (https://get.genotoul.fr/) ...
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bioRxiv - Molecular Biology 2019Quote: ... cDNA synthesis was performed on 1 µg of RNA using ProtoScript First Strand cDNA Synthesis Kit (Promega). Primers recognizing exons flanking the CRISPR-targeted exon (Supp ...
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bioRxiv - Microbiology 2019Quote: ... 1 mL of culture was used for genomic DNA isolation with the Wizard DNA Purification Kit (Promega). Approximately 2 µg of DNA was used to prepare a paired-end 250-bp sequencing library with the Nextera XT DNA library kit ...
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bioRxiv - Biophysics 2022Quote: ... PCR products were separated on 1% agarose gel and purified using a Gel extraction kit (Promega, CA). Sequences of forward and reverse primers are listed in Supplementary Table S21 ...
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bioRxiv - Bioengineering 2023Quote: ... 1 µg of total RNA was used to synthesize cDNA using M-MLV reverse transcriptase kit (Promega) using the supplier’s protocol ...
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bioRxiv - Pathology 2022Quote: ... A total of 1 µg of extracted RNA was transcribed into cDNA using GoScript Reverse Transcription Kit (Promega). Quantitative real-time PCR was performed using FastStart SYBR Green Master mix (Roche ...
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bioRxiv - Developmental Biology 2019Quote: ... HRP coupled secondary antibodies (1/2,000, sigma) were used and blots were developed using an ECL kit (Promega).
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bioRxiv - Cell Biology 2019Quote: DNA from DLD-1 and nucleoporins-targeted cells was extracted with Wizard® Genomic DNA Purification Kit (Promega). Clones were genotyped by PCR for homozygous insertion of tags with two sets of primers listed in Supplementary Table 5.
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bioRxiv - Cell Biology 2020Quote: DNA from DLD-1 and nucleoporins-targeted cells was extracted with Wizard® Genomic DNA Purification Kit (Promega). Clones were genotyped by PCR for homozygous insertion of tags with two sets of primers listed below.
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bioRxiv - Synthetic Biology 2020Quote: ... a 1 mL aliquot was taken for genomic isolation through the Maxwell kit (Promega, Cat# AS1490, Madison WI). Successful integration was verified through qPCR ...
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bioRxiv - Synthetic Biology 2020Quote: ... a 1 mL aliquot was taken for genomic isolation using the Maxwell kit (Promega, Cat# AS1490, Madison WI). Successful integration was verified using qPCR ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ATP release from THP-1 cells was determined by the ENLIGHTEN ATP Assay System Bioluminescence Detection Kit (Promega). Cells were grown in 6-well plates and starved overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... Luciferase gene expression in E0771 and RM-1 cells was validated using a luciferase activity assay kit (Promega). Luciferase-labeled E0771 and RM-1 cells were maintained in RPMI 1640 media supplemented with 10% FBS ...
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bioRxiv - Plant Biology 2023Quote: The substrate of the nano-luciferase diluted at 1:100 (v/v) (furimazine, Kit from Promega (ref 1110)) was spread on seedling that grew on Agar plates ...
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bioRxiv - Microbiology 2024Quote: ... Relative quantification of viral RNA was performed using the GoTaq® 1-Step RT-qPCR System kit (Promega). The mixture contained 5 μL of 2x Master Mix ...
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bioRxiv - Biochemistry 2024Quote: The amount of intracellular Glu was measured in 1×106 cells using the kit Glutamate-Glo (J7021; Promega) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: Kit (Promega). For transposition with an additional plasmid pCas12k ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were lysed after 72 h (2+1 DIV for differentiations) using the Dual Luciferase Reporter kit (Promega, E1960) and luciferase activity was measured using a Victor3 (Perkin Elmer ...
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bioRxiv - Developmental Biology 2019Quote: ... and 1 μg of RNA was reverse-transcribed by the oligo (dT) primer using a reverse transcription kit (Promega) after digestion with RNase-free DNaseI (Fermentas) ...
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bioRxiv - Molecular Biology 2020Quote: ... ZmIBH1-1 was fused to HaloTag using the kit from pFN19K HaloTag T7 SP6 Flexi Vecto (cat#G184A) (Promega). ZmCLA4 fused to HaloTag was expressed using the TnT SP6 High-Yield Wheat Germ Protein Expression System (L3260 ...
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Activation of innate immune cGAS-STING pathway contributes to Alzheimer’s pathogenesis in 5×FAD micebioRxiv - Neuroscience 2022Quote: ... cDNA was generated from 1 μg total RNA per sample using the M-MLV Reverse Transcriptase kit (M1705, Promega). To detect RNA transcript of targeted genes ...
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bioRxiv - Microbiology 2022Quote: ... 1 mL of cells was then processed using the Promega Wizard® genomic DNA preparation kit (Promega, WI, USA) to obtain genomic DNA ...
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bioRxiv - Bioengineering 2019Quote: ... Cultures were grown for 16 h as described above and then 1 mL of culture was used to isolate DNA using the Wizard DNA Purification Kit (Promega). A Nanodrop 2000 (Thermo Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... Nucleic acids deriving from PCRs or restriction digests reactions were purified from 1% TAE agarose gels with the “Wizard SV Gel and Purification System Kit” (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: A random-primers based reverse transcription of 1 μg of RNA was performed with the A3500 Reverse Transcription System Kit (Promega), followed by quantitative real-time PCR on cDNA using the FastStart Essential DNA Green Master kit (Roche ...
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bioRxiv - Cancer Biology 2021Quote: ... The desired PCR fragments were isolated from a 1% agarose gel and purified using the Wizard Genomic DNA Purification Kit (Promega). In order to identify the specific edition ...
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bioRxiv - Molecular Biology 2022Quote: ... The SELENOP 3’UTR and the control plasmid were linearized using Bsb 1 and in vitro transcribed using the Ribomax kit (Promega). The RNA was then purified using p30 size exclusion columns (Bio-Rad ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was eluted from the beads using the elution buffer (1% SDS and 100 mM NaHCO3) and purified by PCR purification kit (Promega). The final products were analyzed by quantitative real-time PCR with the primers listed below:
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bioRxiv - Developmental Biology 2021Quote: ... The PCR amplified inserts were run on a 1% agarose gel and purified using the Wizard SV Gel and PCR Clean-Up System kit (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... between 0.5 to 1 µg of total RNA was reverse transcribed into cDNA using an ImProm II Reverse Transcriptase kit (Promega). Equal amounts of cDNA were quantified by RT-qPCR using the IQ SYBR green Supermix (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: The AP-1 luciferase reporter was expressed using an adenoviral vector and luciferase activity determined using a luciferase assay kit from Promega as previously described (Higazi et al. ...
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bioRxiv - Synthetic Biology 2021Quote: ... Amplification products were checked on a 1% agarose gel and were purified using the Wizard PCR clean-up kit (Promega). DNA concentration and purity were measured using a ND-1000 UV-Vis Spectrophotometer (Nanodrop Technologies).
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bioRxiv - Microbiology 2020Quote: ... PCR reaction mixtures were solved in 1% (w/v) agarose gel and purified by means of Wizard SV gel and PCR Clean Up kits (Promega). The amplified gene was treated with Taq polymerase (Fermentas ...
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bioRxiv - Microbiology 2021Quote: ... as described previously(40) and 1 μg of RNA was reverse transcribed to cDNA using the ImPromIITM Reverse Transcriptase kit (Promega), according to manufacturer’s instructions.
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bioRxiv - Pathology 2021Quote: RT-qPCR (SARS-CoV-2 and MS2 viral genome detection) were performed with the GoTaq 1-step qRt-PCR kit (Promega) using 3.8µL of extracted RNA and 6.2µL of RT-qPCR mix that contains 250nM of each primer and 75nM of probe ...