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Citations for Promega :
101 - 150 of 5849 citations for Cow Eukaryotic Translation Initiation Factor 1 EIF1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Human SMARCB1 was translated in vitro using TNT Quick Coupled Transcription/Translation System (L1170, Promega). 1 µg of pcDNA3.1-FLAG-SMARCB1 was incubated at 30°C for 90 minutes with 20 µM methionine and TNT T7 Quick Master Mix ...
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Membrane integration and topology of RIFIN and STEVOR proteins of the Plasmodium falciparum parasitebioRxiv - Biochemistry 2019Quote: ... Plasmid pGEM1 and TNT SP6 Quick Coupled Transcription/Translation System were purchased from Promega (USA). All enzymes were from ThermoFisher Scientific (USA ...
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bioRxiv - Cell Biology 2019Quote: ... and RXRA proteins were produced using the TNT® Quick Coupled Transcription/Translation System (Promega). Fluorescent probes were synthesized by Integrated DNA Technologies and corresponded to the LXRE1 sequence with IRDye 700 attached to the 5’ end ...
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bioRxiv - Cancer Biology 2020Quote: In vitro translation assay for cMYC was performed by rabbit reticulocyte lysate system (Promega, USA) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: In vitro translation was performed in nuclease-treated rabbit reticulocyte lysates (RRL) (Promega cat #L4960) using a luciferase mRNAs in the presence of the indicated concentrations of (PR)20 ...
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bioRxiv - Cancer Biology 2020Quote: ... In vitro transcription and translation experiments were done with rabbit reticulocyte lysate (TNT systems, Promega) according to the manufacturer’s recommendation ...
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bioRxiv - Biochemistry 2020Quote: ... Radiolabelled PDIP38 preprotein was synthesized using TNT® SP6 Quick Coupled Transcription-Translation System (Promega) with undigested pOTB7/PDIP38 as template and 11 μCi of [35S]Met/CysEXPRE35S35S protein labelling mix (specific activity of >1000 Ci/mmol ...
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bioRxiv - Biochemistry 2022Quote: ... In-vitro translations were done with rabbit reticulocyte lysate (Flexi Rabbit Reticulocyte Lysate System, Promega) and translated CFTR (domains ...
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bioRxiv - Plant Biology 2022Quote: ... the 40 µl in vitro translation reactions contained 20 µM 19 amino acid mix (Promega) and 0.45 µCi/µl of [35S]-methionine (Hartmann Analytics) ...
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bioRxiv - Plant Biology 2022Quote: In vitro translation was carried out using a wheat germ protein expression system (Promega, USA) following the manufacturer’s instruction ...
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bioRxiv - Microbiology 2022Quote: ... expressed using the TNT SP6 High Yield Wheat Germ in vitro transcription/translation system (Promega) in accordance with the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were produced in vitro via the TNT SP6 Quick Coupled Transcription/Translation System (Promega) following manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: Proteins were expressed in rabbit reticulocyte lysate (TNT T7 Quick Coupled Transcription/-Translation System, Promega) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: Proteins for CoIP were produced with the TnT Quick Coupled Transcription/Translation System (Promega, www.promega.com) according to the manufacturer’s recommendations ...
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bioRxiv - Genetics 2022Quote: Proteins were produced in vitro via the TNT SP6 Quick Coupled Transcription/Translation System (Promega). If two proteins were to be tested together in a single binding reaction ...
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bioRxiv - Biochemistry 2023Quote: Proteins labeled with [35S]-methionine were produced by coupled transcription and translation reactions (T7, Promega). Translation products were partially purified by ammonium sulfate precipitation [87] ...
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bioRxiv - Molecular Biology 2020Quote: ... 2.5ng ml-1 recombinant human fibroblast growth factor (rhFGF) (Promega, Cat#G5071, Madison, WI, USA), and 1% chicken embryonic extract (Seralab ...
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bioRxiv - Molecular Biology 2021Quote: ... RRL in vitro translation reactions were carried out according to the manufacturer’s guidelines (Promega, Cat.No. L4960) for 50 min.
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bioRxiv - Cell Biology 2021Quote: ... and proteins were expressed with a TNT Quick Coupled Transcription/Translation System (Promega, Madison, WI, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: IVTT reactions were performed using the SP6 TnT® Quick Coupled Transcription/Translation System (Promega #L2080) according to the manufacturer’s instructions.
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bioRxiv - Plant Biology 2019Quote: ... we used a control plasmid provided in the TnT® Quick Coupled Transcription/Translation System (Promega). The plasmid was linearized using the restriction enzyme SacI and purified using the DNA Clean and Concentrator Kit (Zymo Research) ...
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bioRxiv - Biophysics 2020Quote: ... 3 μL of translation reaction was added to 30 μL Bright-Glo Luciferase assay reagent (Promega) and the resulting luminescence was measured in the illuminometer over a spectral wavelength of 350–650 nm and an integration time of 10 s at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: Proteins were synthesized with the TnT SP6 Quick Coupled Transcription/Translation System (Promega, Madison, Wisconsin, USA).
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bioRxiv - Molecular Biology 2022Quote: ... were incubated with 35S labeled proteins made by using TNT Quick Coupled transcription/Translation System (Promega) with 35S labeled methionine ...
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bioRxiv - Physiology 2022Quote: Synthesis of S35-labled CaVβ chimeras was done by coupled in vitro transcription-translation (Promega (L1170)) ...
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bioRxiv - Biochemistry 2022Quote: ... The mRNA obtained was used for in vitro translation in Flexi Rabbit Reticulocyte Lysate System (Promega) and the resulting lysate was directly used in the import reaction ...
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bioRxiv - Molecular Biology 2023Quote: FLAG-tagged SPUD in vitro expression was done with Transcend Non-Radioactive Translation Detection System (Promega) as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: Sly1-HA3 was synthesized with the TNT® SP6 Quick Coupled Transcription/Translation system (#L2080, Promega), according to the instructions of the manufacturer ...
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bioRxiv - Synthetic Biology 2023Quote: Translation yield from the PURExpress reactions was quantified using Nano-Glo HiBiT Extracellular Detection System (Promega). To make 1X NanoGlo solution ...
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bioRxiv - Cancer Biology 2024Quote: ... the fusion proteins were expressed using the cell-free transcription and translation system (Cat. # L4140, Promega) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... and Jurkat T cells overexpressing hPD-1 and carrying a luciferase reporter gene under the control of Nuclear Factor of Activated T-cells Response Element (NFAT-RE) (hPD-1 Effector Cells, hPD-1 ECs, Promega) were cultured in RPMI-1640 medium (Biowest ...
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bioRxiv - Molecular Biology 2020Quote: In vitro transcription/translation assays were carried out in a S30 T7 HighYield Protein Expression System (Promega). The full lenght Hrnpk cDNA template was cloned from total RNA obtained from GH4C1 cells and transferred to a pET28a Escherichia coli T7 expression vector (Novagen ...
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bioRxiv - Genetics 2019Quote: ... The resulting BMP15 protein was revealed using Transcend non-radioactive translation detection system with chemiluminescent method (Promega) after reducing SDS-PAGE on a gradient (4-15% ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were in vitro translated using TnT Quick Coupled Transcription/Translation System (Promega, USA, Cat. No. L1170) according to the manufacturer’s protocol.
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bioRxiv - Plant Biology 2020Quote: ... 10 µl of the translation reaction product was mixed into 50 µl of Luciferase assay reagent (Promega) and measured immediately on a GloMaxTM20/20 Luminometer (Promega) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and 700 ng used for in vitro transcription/translation using TnT T7 Quick for PCR DNA (Promega) and the Fluorotect GreenLys tRNA (Promega ...
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bioRxiv - Cell Biology 2020Quote: KNL-2-MBP fragments were [35]S labeled using TnT® Quick Coupled Transcription/Translation System (Promega). 10 μl of the in vitro translation lysate was incubated for 1hr at 4°C with 50 μg CENP-AHCP-3(1-109)-MBP-His (in 20 mM Tris [pH7.5] ...
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bioRxiv - Cell Biology 2021Quote: Proteins labeled with [35S]-methionine were produced by coupled transcription and translation reactions (SP6 or T7, Promega). Translation products were partially purified by ammonium sulfate precipitation (Bardwell & Shah ...
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bioRxiv - Immunology 2021Quote: ... and used to generate 35S-methionine labelled AIM2 protein by in vitro transcription and translation (IVTT) (Promega). Immunoprecipitations (IP ...
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bioRxiv - Cell Biology 2022Quote: Constructs for in vitro translations in rabbit reticulocyte lysate were based on the pSP64 vector (Promega, USA). Constructs for in vitro translation in the E ...
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bioRxiv - Physiology 2023Quote: ... 25 ng/mL fibroblast growth factor (bFGF; Promega), 1% penicillin streptomycin ...
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bioRxiv - Cell Biology 2020Quote: ... and biotinylated in a single reaction with the TnT® SP6 Quick Coupled Transcription/Translation System (Promega, L2080) and Transcend™ tRNA (Promega L5016) ...
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bioRxiv - Molecular Biology 2020Quote: In vitro transcription/translation of vvl/dfr vDNA was carried out using TNT coupled reticulo lysate system (Promega) and T3 RNA polymerase (New England Biolabs Inc. ...
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bioRxiv - Biochemistry 2020Quote: ... Lep proteins were then produced from mRNA by in vitro translation using rabbit reticulocyte lysate (Promega, Madison, WI) supplemented with canine pancreatic rough microsomes (tRNA probes ...
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bioRxiv - Biochemistry 2020Quote: ... were transcribed and translated using the TNT T7 Quick for PCR DNA Coupled Transcription/Translation System (Promega, USA). The reactions contained 10 µL of TNT ...
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bioRxiv - Microbiology 2021Quote: In vitro transcription and translation reactions were conducted using the TNT coupled rabbit reticulocyte lysate system (Promega, L4610) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... 2μl of in vitro translation product was then mixed with 25μl of nano luciferase assay reagents (Promega N205A). Light production was measured on a luminometer ...
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bioRxiv - Biochemistry 2024Quote: ... In vitro translations were performed with wheat germ extract as directed by the manufacturer (Promega or tRNA Probes) with 3H leucine or 35S methionine ...
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bioRxiv - Cell Biology 2023Quote: ... A direct cAMP ELISA kit (Enzo) was used in endpoint experiments and the pGloSensor (−20F) luminescence assay (Promega) was used for kinetic cAMP measurements ...
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bioRxiv - Molecular Biology 2019Quote: ... and C-terminally truncated VP16 (VP16ΔC) proteins were synthesized using a rabbit TNT quick-coupled transcription/ translation system (Promega) with 35S-labeled methionine ...