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Citations for Promega :
301 - 342 of 342 citations for Collagen Binding Fragment since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: BBSome subunits and fragments thereof were translated in vitro from pCS2-Myc plasmids using the TNT SP6 Quick Coupled Transcription/Translation system (Promega L2080). 16 µL TNT SP6 Quick Master Mix ...
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bioRxiv - Cancer Biology 2019Quote: A wild-type or mut-hsa-miR-329-5p fragment was constructed and inserted downstream of the luciferase reporter gene of the pmirGLO Vector (Promega, USA). We used ExFect 2000 Transfection Reagent to transfect the reporter plasmid into 293T cells ...
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bioRxiv - Molecular Biology 2019Quote: ... The purified PCR-amplified promoter fragments (∼1 kb) were cloned between KpnI and XhoI sites in firefly luciferase reporter vector pGL3-Basic (Promega, USA). The resulting plasmids were named as SHR-Hmgcr and WKY-Hmgcr which contain −985 bp to +20 bp region of SHR and WKY Hmgcr promoter sequence ...
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bioRxiv - Cell Biology 2021Quote: ... PCR fragments amplified from genomic DNA extracted from the different founder mice using the Wizard SV genomic DNA extraction kit (Promega, A2360), were cloned into a vector using the TOPO TA cloning kit (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2020Quote: ... The resulting fragments were ligated to Nextflex 6bp adaptors (Bio Scientific, #514122) with the use of T4 DNA ligase (Promega, M1804). The libraries were amplified using an AT-rich optimized KAPA protocol using KAPA HiFi HotStart ready mix (KAPA Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... The amplified signal peptide fragments were introduced into the plasmid pSUC2 (Prof. Sophien Kamoun, The Sainsbury Laboratory) using T4 DNA ligase (Promega, USA). The yeast invertase assay was used to validate the signal peptides (Jacobs et al ...
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bioRxiv - Systems Biology 2020Quote: ... we cloned synthetic fragments of HERV DNA (sequences in Table S8) using gBlocks® (Integrated DNA Technologies) into psiCHECK-2 (Promega). The fragments were inserted downstream of the Renilla luciferase gene using XhoI and NotI restriction sites and DNA ligation ...
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bioRxiv - Pathology 2021Quote: ... The DNA-A and DNA-B fragments were purified and cloned into the pGEM-T Easy vector (Promega, Madison, WI, USA) and then transformed into Escherichia coli strain DH5α cells by the heat shock method ...
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bioRxiv - Cell Biology 2019Quote: ... were generated using PCR amplification of two DNA fragments and subsequent assembly PCR (using Pfu DNA polymerase from Promega, Mannheim, Germany) with the artificially inserted exon 9 sequence as region for annealing ...
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bioRxiv - Genetics 2021Quote: ... 5 μL of substrate was added to a final concentration of 100 nM 11S fragment and 0.5X furimazine (Promega, NanoGlo Substrate). Luminescence was measured on a ViewLux HTS reader (PerkinElmer ...
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bioRxiv - Cell Biology 2021Quote: ... Myc tagged full-length and truncated ARL13B fragments were generated by in vitro translation using TNT Sp6 high-yield wheat germ protein expression system (Promega; L3261) from pCS2-Myc-ARL13B vectors generated by gateway cloning ...
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bioRxiv - Genetics 2021Quote: ... All PCR fragments were digested with KpnI and HindIII restriction enzymes and ligated with pGL3-Basic vector (Promega, Madison, WI, USA).
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bioRxiv - Genomics 2022Quote: On- and off-target reporters were generated by sub-cloning a DNA fragment containing a fully complementary (23-mer) or partial siRNA target sites into the psiCHECK2 vector (Promega C8021) between Xho1 and Not1 restriction sites ...
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bioRxiv - Physiology 2022Quote: The prepared PCR fragments were inserted into the pGEM-T vector via T/A cloning following the manufacturer’s recommendations (Promega, Fitchburg, USA). Competent Escherichia coli were transfected with the ligation mix and plated on agar plates as described previously [13] ...
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bioRxiv - Biophysics 2023Quote: ... Subsequently we ran the digested fragment on a 1% TAE agarose gel and the desired ∼14 kbp DNA fragment was isolated from an agarose gel using a gel purification kit (Promega, A9282).
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bioRxiv - Cancer Biology 2023Quote: ... Assessment of DNA fragment length was performed by electrophoretic separation in a 0.4% agarose gel with DNA ladder (Promega, WI, USA).
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bioRxiv - Cell Biology 2023Quote: ... ligation of double digested fragment of either domain with double digested pAcGFP1-N1 was performed with the LigaFAst Rapid DNA Ligation System (Promega, M8226) following the manufacturer’s suggested protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were stably transduced with a lentivirus-based expression construct generated by cloning a polymerase chain reaction (PCR)-amplified DNA fragment encoding luciferase from a pGL4.10-lucIIplasmid (Promega, Sydney, Australia) into a pLenti CMV Hygro DEST vector (Addgene ...
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bioRxiv - Biophysics 2023Quote: ... from the access handles by running this on a 1% agarose gel and gel purify the right DNA fragment with a gel purification kit (Promega, A9282).
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bioRxiv - Biochemistry 2023Quote: ... and the DNA fragments encoding the designs were amplified following a colonyPCR protocol using GoTaq® Green DNA polymerase master mix (#M7122; Promega) and T7 reverse and forward primers ...
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bioRxiv - Microbiology 2023Quote: ... The three PCR fragments were ligated by PCR using primers P1/P3 and cloned into pGEM®-T easy vector (Promega). Plasmid was then digested with NotI ...
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bioRxiv - Microbiology 2023Quote: ... The amplified PCR fragments were checked for uniformity and size using 0.8% agarose gel electrophoresis and purified using a PCR fragment recovery kit (Promega, WI, USA). The recovered DNA fragments were sequenced using the MiSeq 500PE v.2 kit (Illumina ...
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bioRxiv - Biochemistry 2023Quote: ... The linearized pMRNAXP vector and the PCR fragments of DNA encoding spike were cleaned up by Wizard® SV Gel and PCR Clean-Up System (Promega). The PCR fragments of spike protein DNA were cloned into linearized pMRNAXP vector using In-Fusion® HD Cloning Kit (Clontech® Laboratories ...
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bioRxiv - Genetics 2019Quote: ... DNA fragments were separated by agarose-gel electrophoresis and purified with the Wizard SV Gel and PCR Clean-Up System (Promega, Madison, USA). Sequence authenticity was confirmed at the Massey Genome Centre ...
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bioRxiv - Neuroscience 2022Quote: ... The wild-type and mut-circ_0002538 fragments were cloned downstream of the luciferase reporter gene of the pMIR-Report vector (Promega, Madison, WI, USA), while wild-type and mut-PLLP 3c-UTRs were inserted downstream of the hRluc (Renilla ...
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bioRxiv - Microbiology 2020Quote: ... 3’-untranslated region (3’-UTR) fragment of IκBα (human/rat) was inserted into the pGL3-basic vector (firefly luciferase; Promega, Madison, WI, USA), which was obtained from General Biosystems (Anhui ...
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bioRxiv - Neuroscience 2021Quote: RNA was extracted from the dissected fragments of tissue with the use of ReliaPrep RNA Tissue Miniprep System (Promega, Madison, WI, USA). The obtained RNA was stored in RNase-free water at −80°C ...
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bioRxiv - Neuroscience 2021Quote: ... The LINE-1 promoter-luciferase reporter plasmid was constructed by subcloning the promoter fragment into the KpnI-XhoI restriction site of the pGL4.11[luc2P] vector (Promega, Cat No. #E6661). The plasmid was sequenced and verified that the correct sequence was cloned.
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bioRxiv - Molecular Biology 2022Quote: ... SARS-CoV-2 5’-UTRC75G and SARS-CoV-2 5’-UTRC75G + G61C gBlocks™ DNA fragments (Integrated DNA Technologies) were cloned immediately upstream of fLuc into pGL3-fLuc plasmids (Promega, #E1751). Cells were transfected as per manufacturer protocol (Polyplus ...
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bioRxiv - Microbiology 2023Quote: ... The 650 bp positive fragment was purified by Wizard® SV Gel and PCR Clean-Up System (Promega Corporation, Madison, WI, USA).
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bioRxiv - Evolutionary Biology 2023Quote: ... one microliter volumes of Reverse Transcription reactions were used as template for amplification of internal cDNA fragments using in 20 μl volume PCR reactions (35 rounds of amplification using Promega 2X PCR Master Mix (Promega, Madison, WI). Gene-specific primers and annealing temperatures are listed in Table 1 ...
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bioRxiv - Plant Biology 2023Quote: ... The 16S rDNA PCR-amplified fragments were purified by Wizard® SV Gel and PCR Clean-Up System (Promega, New Delhi, India) and digested by HaeIII (SRL ...
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Multi-omics analysis reveals signatures of selection and loci associated with complex traits in pigsbioRxiv - Genomics 2023Quote: ... 400-bp DNA fragments centring on the SNPs were first amplified and then inserted into the PGL4.23 Dual-Luciferase Expression Vector (Promega, Madison, WI, USA). Primers were designed using Primer Premier5 and listed in Supplementary Table 21.
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bioRxiv - Physiology 2024Quote: ... target cDNA fragments were amplified with the primers listed in Table 1 and ligated into pGEM T-Easy vector (Promega, Madison, WI). The cDNA fragments were PCR amplified with M13 primers and purified with the FastGene Gel/PCR Extraction Kit (Nippon Genetics ...
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bioRxiv - Molecular Biology 2020Quote: ... The 3’ UTR fragment was digested with Xba I and inserted downstream of the firefly luciferase gene in pGL3-control (Promega, Madison, WI, USA), yielding pGL3covi-3UTR_Luc ...
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bioRxiv - Microbiology 2021Quote: ... The linearized vector and the slpMh-6x His fragment were gel-purified using the Wizard® SV Gel and PCR Clean-Up System (Promega, Mannheim, Germany) and assembled via isothermal in vitro ligation according to Gibson et al ...
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bioRxiv - Synthetic Biology 2023Quote: ... backbone to contain the large or small fragments of the NanoBiT luciferase (Large BiT or Small BiT) used for the split luciferase complementation assay (Promega, Madison, WI, USA)38 ...
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bioRxiv - Microbiology 2022Quote: ... The resulting suspension was then centrifuged at 14,000 rpm at 4°C to remove cellular fragments and the genomic DNA finally was extracted using the Wizard® Genomic DNA Purification Kit reference A1125 (Promega, Madison, WI, USA) following the bulla guidelines ...
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bioRxiv - Microbiology 2022Quote: ... PCR bands at the correct fragment sizes were excised from the gel and extracted using Wizard® SV Gel and PCR Clean-Up System (Promega Corporation, Madison WI). PCR products were quantified using a qubit 2.0 fluorometer (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... Bands at the correct fragment sizes were excised from the gel and extracted using Wizard® SV Gel and PCR Clean-Up System (Promega Corporation, Madison WI). PCR products were quantified using a qubit 2.0 fluorometer (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... PCR bands at the correct fragment sizes were excised from the gel and extracted using Wizard® SV Gel and PCR Clean-Up System (Promega Corporation, Madison WI). PCR products were quantified using a qubit 2.0 fluorometer (Invitrogen ...
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bioRxiv - Genomics 2020Quote: ... All of these sequences were synthesized as gBlocks® Gene Fragments (Integrated DNA Technologies, Coralville, IA, USA) and cloned into pGL3::PRO (Promega Corporation, Madison, WI, USA) downstream of the luciferase gene ...