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Citations for Promega :
1 - 50 of 342 citations for Collagen Binding Fragment since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... of SNHG16 sequence and the 3’-untranslated region (UTR) fragment of STARD9 containing miR-1301-3p binding site were subcloned into the pmirGLO vectors (Promega, Madison, WI, USA) to generate SNHG16-Wt/Mut vectors and STARD9-Wt/Mut vectors ...
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bioRxiv - Biochemistry 2021Quote: ... Halo-tagged ubiquitin-binding domains (UBDs) of TUBE (tandem-repeated ubiquitin-binding entities) was incubated with HaloLink resin (200 μL, Promega) in binding buffer (50 mm Tris⍰HCl ...
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bioRxiv - Bioengineering 2021Quote: ... and collagen content via a QuantiFlour dsDNA assay kit (Promega), a modified 1,9-dimethylmethylene blue (Sigma-Aldrich ...
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bioRxiv - Physiology 2023Quote: ... the oligo with NF-κB consensus binding element (Promega) was end-labeled by T4 polynucleotide kinase (Promega ...
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bioRxiv - Neuroscience 2021Quote: ... the Gbait-hsp70 fragment and QF2 fragment were independently inserted into pGEM T-easy (A1360, Promega) and subsequently combined into one vector by SacII digestion and ligation (Addgene ...
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bioRxiv - Bioengineering 2022Quote: ... and collagen content were assessed via Quantifluor dsDNA assay kit (Promega), a modified 1,9-dimethylmethylene blue (DMMB ...
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bioRxiv - Biophysics 2023Quote: ... Collagens were then digested using Sequencing Grade Modified Trypsin from Promega with a ratio of 1:50 (trypsin ...
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bioRxiv - Bioengineering 2023Quote: ... and collagen content were then determined via Quantifluor dsDNA assay kit (Promega), modified 1,9-dimethylmethylene blue (DMMB ...
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bioRxiv - Immunology 2022Quote: ... Nonspecific binding was blocked with PBS+ 0.5% Tween® 20 (Promega) + 10% FBS for 2 hours at RT ...
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bioRxiv - Developmental Biology 2020Quote: ... DNA fragments were amplified using GoTaq (Promega) for 40 cycles under the following conditions ...
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bioRxiv - Developmental Biology 2022Quote: ... DNA fragments were amplified using GoTaq (Promega) for 40 cycles to detect null or WT alleles under the following conditions ...
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bioRxiv - Biochemistry 2021Quote: ... The fragments were digested with BglII (Promega) and purified with E.Z.N.A ...
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bioRxiv - Neuroscience 2023Quote: Split nanoluc fragments LgBit and SmBit (Promega) were cloned N-terminally to human CISD1 and point mutations introduced by site-directed mutagenesis using the Q5 Site-Directed Mutagenesis kit (NEB) ...
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bioRxiv - Neuroscience 2021Quote: ... The binding proteins were eluted by trypsin (Promega Corporation, Madison, WI, USA) for 1 hour at 37°C ...
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bioRxiv - Bioengineering 2021Quote: ... a volume of binding buffer from PCR clean-up kit (Promega, A9282) was added followed by 2 µL (300 ng ...
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bioRxiv - Molecular Biology 2021Quote: ... CANX fragments were cloned into pmirGlo (Promega, E1330) using In-Fusion HD Cloning Kit (Takara ...
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bioRxiv - Biochemistry 2021Quote: ... the CD fragments were digested with BglII (Promega) and BamHI (New England Biolabs ...
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bioRxiv - Biophysics 2022Quote: ... the two purified flanking PCR fragments (Promega, USA) were assembled with BamHI and HindIII-digested PK18 (Gmr ...
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bioRxiv - Genomics 2019Quote: ... Amplified fragments were cloned into pGL4.23 plasmid (Promega), which consists of a minimal promoter and the firefly luciferase reporter gene ...
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bioRxiv - Developmental Biology 2022Quote: ... Fragments were cloned into pGEMt-easy (Promega #A1360) and sequenced.
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bioRxiv - Molecular Biology 2023Quote: ... PCR fragments were subcloned into pGEM-Teasy (Promega) and then moved as HindIII/NotI fragments into pCB4699 ...
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bioRxiv - Developmental Biology 2024Quote: ... PCR fragments were cloned in pGEM-T (Promega), and the inserts were sequenced by Sanger sequencing (University of California Berkeley DNA Sequencing Facility) ...
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bioRxiv - Biophysics 2022Quote: ... The resulting 2.6-kb fragment was separated from the 1.4-kb fragment and uncut plasmid by agarose gel purification (Promega Gel Wizzard Kit). A set of oligonucleotides that form a biotinylated and primed fork was ligated to one end of the fragment and the final product purified on a Sepharose-4B column as previously described [42] ...
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bioRxiv - Cell Biology 2020Quote: ... based on a mutated RIIB cAMP-binding domain from PKA (pGloSensor-20F, Promega), which produces rapid and reversible cAMP-dependent activation of luciferase activity in intact cells and is capable of detecting cAMP elevations in the absence of phosphodiesterase inhibitors ...
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bioRxiv - Cancer Biology 2023Quote: ... and E2 DNA-binding sites) and to pSV40-luc (pGL3-Control from Promega, containing the SV40 promoter and enhancer regions but no E2 DNA-binding sites) ...
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bioRxiv - Cell Biology 2022Quote: ... the IRES fragment was amplified from pTUNER-IRES (Promega) with primers IRES10 + IRES11 ...
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bioRxiv - Cell Biology 2022Quote: ... the IRES fragment was amplified from pTUNER-IRES (Promega) with primers IRES10 + IRES11 ...
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bioRxiv - Microbiology 2022Quote: ... This fragment was ligated into the pSP72 vector (Promega) using T4 DNA ligase using the EcoRI and XbaI site on its polylinker ...
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bioRxiv - Developmental Biology 2023Quote: ... DNA was spiked with unmethylated lambda phage fragments (Promega) and sonicated to approximately 300 base pairs ...
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bioRxiv - Microbiology 2020Quote: ... Predicted binding sites were cloned and inserted into the pmirGLO vector (Promega, Madison, USA). For reporter assays ...
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bioRxiv - Developmental Biology 2020Quote: ... and 200 ng purified recombinant mouse DLX2 protein in Gel Shift Binding Buffer (Promega). Unlabeled probe was added for ‘cold’ competition ...
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bioRxiv - Biochemistry 2020Quote: ... binding was performed between the soluble fraction (supernatant) and glutathione-conjugated magnetic beads (Promega) pre-equilibrated with lysis buffer ...
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bioRxiv - Developmental Biology 2021Quote: ... DNA fragments were cloned into pGEM-T Easy Vector (Promega). DNA templates for in vitro transcription were amplified using M13F and M13R primers ...
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bioRxiv - Evolutionary Biology 2019Quote: ... This fragment was ligated into pGEM-T easy (A1360, Promega) for storage ...
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bioRxiv - Plant Biology 2020Quote: ... The fragments were cloned into pGEM-T easy vector (Promega) for sequencing ...
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bioRxiv - Microbiology 2020Quote: ... DNA fragments were amplified using either GoTaq DNA Polymerase (Promega) for diagnostic purposes or the proofreading Phusion High-Fidelity DNA Polymerase (NEB ...
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bioRxiv - Plant Biology 2020Quote: ... these fragments were alternatively cloned into pGEM-T (Promega, USA) or into pENTR (Themo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... a PCR fragment was amplified using GoTaq DNA polymerase (Promega) and subcloned in a TOPO cloning vector (Invitrogen) ...
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bioRxiv - Molecular Biology 2020Quote: ... the PCR fragments were cloned into the psiCHECK2 vector (Promega) downstream of Renilla luciferase ...
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bioRxiv - Molecular Biology 2020Quote: ... The PCR fragment was cloned into pGEM-T vector (Promega) and used for the synthesis of cRNA probe using T3 and T7 RNA polymerases (Stratagene ...
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bioRxiv - Neuroscience 2019Quote: ... Amplified fragments were cloned into pCI expression vectors (Promega E1731) containing the first 20 residues of human rhodopsin (Rho-pCI ...
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bioRxiv - Molecular Biology 2022Quote: ... the fragments were transcribed with the T7 RNA Polymerase (Promega) at 37°C over night ...
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bioRxiv - Cancer Biology 2022Quote: ... the fragment was subcloned into the pGL4.26 luciferase reporter (Promega) using cloning sites Xho I and Bgl II ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR amplified fragments along with O’ Generuler 1kb ladder (Promega) were resolved using 1.2% of 10X diluted GelRed™ (Biotium Inc. ...
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bioRxiv - Biochemistry 2022Quote: ... the binding solution contains 1.0 µl of NanoLuc substrate stock solution (Promega, Madison, WI, USA). After addition to one well ...
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bioRxiv - Physiology 2023Quote: ... The DNA binding activity of NF-κB was assayed according to the protocol from Promega Corp ...
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bioRxiv - Bioengineering 2023Quote: ... 2.25 ng/uL Single-Stranded DNA Binding Protein (Sigma, cat# S3917 OR Promega, cat# M301A). 12.5 uL of master mix was mixed with 12.5 uL of 2x input oligonucleotide ...
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bioRxiv - Biochemistry 2024Quote: ... an apo-TDO2 binding molecule (structure not disclosed) was coupled to the NanoBRET 590SE (Promega) dye to create a fluorescent tracer ...
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bioRxiv - Plant Biology 2020Quote: ... The amplified fragment was cloned into pGEMT-Easy vector system (Promega) and then sub-cloned into the EcoRI and BamHI restriction enzyme (RE ...
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bioRxiv - Microbiology 2021Quote: ... Amplified promoter fragments were ligated into the pGEM-T Easy (Promega) and transformed into E ...