Labshake search
Citations for Promega :
51 - 100 of 1614 citations for Chikungunya Virus E2 Envelope Protein HEK293 Human Fc Tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2021Quote: HEK293 stably expressing pcDNA3.1(+)_SSF-GCGR cells transfected with GloSensor20F (Promega) were lifted and resuspended in imaging media (DMEM without phenol red supplemented with 30 mM HEPES ...
-
bioRxiv - Systems Biology 2021Quote: ... HEK293 cells were co-transfected using Fugene 6 transfection reagent (Promega) with MAC-tag (600ng ...
-
bioRxiv - Immunology 2021Quote: ... and HHEX (NM_002729) attached to a FLAG tag at the 5’ end and HiBit tag (Promega, Madison, WI) at the 3’ end ...
-
bioRxiv - Biophysics 2021Quote: ... and Halo-Tag (O4) ligand (Promega, P6741). To avoid non-specific interaction the glass chambers were washed with blocking buffer (20 mM Tris-HCl ...
-
bioRxiv - Cell Biology 2021Quote: ... JF646-conjugated Halo Tag ligand (Promega, GA1120) was added to culture at a final concentration of 200 nM and cells were incubated for 30 mins before live-imaging ...
-
bioRxiv - Biophysics 2022Quote: ... Then Halo-Tag (O4) ligand (Promega, P6741) were passed and incubated overnight at room temperature ...
-
bioRxiv - Bioengineering 2019Quote: ... Halo Tag Alexa Fluor 488 Ligand (Promega) was diluted with moderate amount of PBS ...
-
bioRxiv - Microbiology 2023Quote: ... Mouse anti-HiBiT tag mAb (30E5, Promega) and goat anti-mouse IgG H&L conjugated with Alexa Fluor 568 (Abcam ...
-
bioRxiv - Biochemistry 2023Quote: ... with the C-terminal HiBiT-Tag (Promega), using the In-Fusion cloning kit ...
-
bioRxiv - Cell Biology 2021Quote: ... conducted with Moloney murine leukemia virus reverse transcriptase (Promega) and random hexamers (Roche Applied Science ...
-
bioRxiv - Neuroscience 2023Quote: ... and Moloney murine leukemia virus MMLV reverse transcriptase (Promega) was performed according to the vendor’s protocol.
-
bioRxiv - Molecular Biology 2020Quote: HEK293 cells were rinsed and lysed with Passive Lysis Buffer (PBL, Promega), protein concentrations determined using the DC protein assay (Bio-Rad) ...
-
bioRxiv - Developmental Biology 2021Quote: ... retroviral plasmid was transfected to HEK293 cells using FuGENE 6 (Promega, E2692). Two days after transfection ...
-
bioRxiv - Molecular Biology 2022Quote: HCT116 cells were transfected using JetPrime(Polyplus) and HEK293 using Fugene (Promega) using a standard protocol ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Plasmid DNAs were transfected into HEK293 cells with ViaFect Transfection Reagent (Promega) according to manufacturer’s instruction.
-
bioRxiv - Physiology 2023Quote: ... whereas HEK293 cells stably expressing a luminescent cAMP GloSensor (GS-293) (Promega) were previously created in our lab 25 ...
-
bioRxiv - Neuroscience 2019Quote: ... and mouse Cbln2 E2 fragments were PCR-amplified from genomic DNA of individual species and inserted into pGL4.24 vector (Promega). The sequences of the PCR primers and synthetic oligonucleotides are listed in Supplementary Table 1.
-
bioRxiv - Molecular Biology 2020Quote: ... Recombinant Halo-tag PUM1 RBD (aa 828-1176) and Halo-tag PUM2 RBD (aa 705-1050) were expressed from plasmid pFN18A (Promega) in KRX E ...
-
bioRxiv - Neuroscience 2019Quote: ... HEK293 cells were transfected in the 24 well plates using Fugene HD (Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... Retrovirus was produced through transfection of HEK293 cells using FuGENE 6 (Promega, E2692). Two days after transfection ...
-
bioRxiv - Molecular Biology 2024Quote: ... 25,000 HEK293-T cells were seeded and transfected with FuGENE 6 (#E231A, Promega) and plasmid DNA at the carrier (µL):DNA (µg ...
-
bioRxiv - Cancer Biology 2022Quote: ... Halo tag TMR Direct Ligand (Promega, cat # G2991) was used for staining of Halo tag transfected fixed cells ...
-
bioRxiv - Cancer Biology 2022Quote: ... Halo-tag (G9211) antibody was purchased from Promega. The C11orf53 antibody was produced in rabbit in house by using full-length C11orf53 recombinant protein as antigen.
-
bioRxiv - Molecular Biology 2023Quote: ... rabbit polyclonal anti-Halo Tag antibody (G928A; Promega), and mouse monoclonal anti-GAPDH antibody (M171-3 ...
-
bioRxiv - Molecular Biology 2024Quote: ... with Halo-Tag TMRDirect ligand (Promega, WI, USA), as per the manufacturer’s guidelines ...
-
bioRxiv - Bioengineering 2022Quote: ... Virus growth was measured with NanoGlo Luciferase Assay System (Promega) and cell viability was measured with CellTiter-Glo 2.0 Cell Viability Assay (Promega) ...
-
bioRxiv - Biophysics 2022Quote: ... 60 µm wide (diameter at 1/e2 peak intensity) in the sample plane as characterised using a sample of immobilised standard fluorescent microbeads (Promega). The source power was 30 mW ...
-
bioRxiv - Neuroscience 2019Quote: ... genomic DNA from puromycin selected cells and wild-type HEK293 cells was extracted (Promega Wizard Genomic DNA Purification Kit #A1120 ...
-
bioRxiv - Neuroscience 2022Quote: HEK293 cells (ATCC, Manassas, VA) stably expressing both D3R and GloSensor 22F-cAMP (Promega) were created for these experiments ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... HEK293 cells were transfected with the VHL-NanoLuc fusion constructs using FuGENE HD (Promega) according to the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2023Quote: HEK293-µOR cells were transfected with a plasmid encoding pGloSensor-20F cAMP reporter (Promega). Cells were harvested 24 h post transfection and resuspended at a 1.5x10^6 live cells/ml in assay media (DMEM without phenol red or FluoroBrite DMEM ...
-
bioRxiv - Biochemistry 2021Quote: ... which contains a pRSET backbone with an N-terminal 6xHis tag and the insertion of a biotin tag from the PinPoint™ Xa-1 Vector (Promega, USA) in between the His tag and the first fluorescent protein ...
-
bioRxiv - Microbiology 2019Quote: ... 200 U of Moloney murine leukemia virus reverse transcriptase (MMLV; Promega) in 30 mM Tris and 20 mM KCl (pH 8⋅3 ...
-
bioRxiv - Cell Biology 2020Quote: ... Virus DNA liposomes were prepared using FuGENE HD Transfection reagent (Promega) and DMEM ...
-
bioRxiv - Biochemistry 2021Quote: ... and 1 unit/μl Avian Myoblastosis virus reverse transcriptase (Promega®) were added and incubated for 1h at 30°C ...
-
bioRxiv - Microbiology 2023Quote: ... Virus infectivity was measured by β-galactosidase activity (Promega, Madison, WI). Virus stocks were concentrated (20X ...
-
bioRxiv - Microbiology 2023Quote: Virus neutralization was measured using a β-galactosidase-based assay (Promega) with TZM.bl target cells (85) ...
-
bioRxiv - Cell Biology 2023Quote: ... and reverse-transcribed using Moloney murine leukemia virus reverse transcriptase (Promega). cDNA was amplified in a thermal cycler (GeneAmp PCR System 9700 ...
-
bioRxiv - Cancer Biology 2024Quote: ... Moloney Murine Leukemia Virus Reverse Transcriptase RNase H-Point Mutant (Promega) was used for cDNA synthesis ...
-
Cytoplasm localized ARID1B promotes oncogenesis in pancreatic cancer by activating RAF-ERK signalingbioRxiv - Cell Biology 2019Quote: Halo tag (Halo pulldown and labeling kit, Promega Corporations) and Streptavidin bead (GE Health care Bio-Science ...
-
bioRxiv - Zoology 2021Quote: ... 10μL Go-tag qPCR Master Mix (Promega, Wisconsin, USA), 0.2μL of forward primer ...
-
bioRxiv - Cell Biology 2021Quote: ... To detect HALO tag 0.5µM Janelia Fluor 549 (Promega) dye or HaloTag® TMR Ligand (Promega ...
-
bioRxiv - Biophysics 2023Quote: ... followed by Halo-Tag amine (O4) ligand (Promega, P6741). The chambers were then treated with a blocking buffer (20 mM Tris-HCl of pH 7.4 ...
-
bioRxiv - Microbiology 2019Quote: ... 625 ng of lentiviral packaging vector psPAX and 625 ng of envelope vector pMD.2G (both gifts from S. Karniely, Weizmann Institute, Israel) using transfection reagent FuGene6 (Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: ... In vitro transcribed RNA derived from plasmid “pCI/SARS-CoV envelope” was synthesized using T7 RiboMAX Express Large Scale RNA production system (Promega), then purified by phenol/chloroform extractions and two successive precipitations with isopropanol and ethanol ...
-
bioRxiv - Immunology 2020Quote: ... 293T cells were co-transfected with envelope-expressing plasmid and an env-deleted HIV-1 backbone plasmid (pSG3ΔEnv) using a FuGENE6 transfection kit (Promega Inc.). Cell supernatants containing pseudotyped viruses were harvested 48 h post transfection and used for infection in TZM-bl cells using DEAE-dextran (25 μg/ml ...
-
bioRxiv - Cell Biology 2023Quote: Luciferase screening of Envelope subgenomic siRNA potentency and specificity was performed using the Dual-Luciferase Reporter Assay System (Promega, E19190). For initial screening of siRNA potency ...
-
bioRxiv - Cell Biology 2020Quote: ... HEK293 cells were treated for 8 hours with either 0.1 µmoles/L Coumermycin A1 (Promega) or an equivalent volume of DMSO ...
-
bioRxiv - Microbiology 2022Quote: ... HEK293 cells were transfected using FuGeneHD transfection reagent according to manufacturer’s protocol (Promega, Cat # E2311). 48 hours post-transfection ...
-
bioRxiv - Microbiology 2020Quote: ... HEK293 cells were transfected using FuGeneHD transfection reagent according to manufacturer’s protocol (Promega, Cat # E2311). 48 hours post-transfection ...