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Citations for Promega :
301 - 350 of 1614 citations for Chikungunya Virus E2 Envelope Protein HEK293 Human Fc Tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... 4 × Promega Wizard® Minicolumns per replicate with a ratio of 0.5:1 virus concentrate to Wizard® PCR Preps DNA Purification Resin (Promega: A7170). The resultant extract was found to be inhibitory to all enzymatic reactions ...
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bioRxiv - Cell Biology 2021Quote: ... The primary MEFs were immortalised by transfection of 2 μg of simian virus 40 large T-antigen-expressing vector employing Fugene 6 Transfection Reagent (Promega, E2311) followed by five rounds of a 1 in10 split to achieve 1/100,000-fold splitting ...
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bioRxiv - Microbiology 2022Quote: ... We performed duplicate serial dilutions using supernatants collected from the virus rescues and measured luciferase expression at each dilution using Bright-Glo Luciferase Assay System (Promega, E2610). Virus titers were calculated as relative light units (RLU ...
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bioRxiv - Molecular Biology 2023Quote: ... Produced pseudoviruses were titrated on HEK-293T-ACE2 by performing duplicate serial dilutions and virus titers were measured 48 hours after infection using Bright-Glo Luciferase Assay System (Promega, E2610).
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bioRxiv - Molecular Biology 2023Quote: ... Cells were incubated at 37 °C for two days and the nLuc activity from the virus replication was measured by using Nano-Glo® Luciferase Assay System (Promega) following the manufacturer’s protocol using a plate reader (HT4 Biotek) ...
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bioRxiv - Immunology 2024Quote: ... The virus neutralization titers were determined by measuring the NLuc activity using the Nano-Glo Luciferase Assay System (Promega, Madison, WI) following the manufacturer’s conditions ...
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bioRxiv - Biochemistry 2022Quote: ... The scFv gene was then ligated into pCSE2.6-hIgG1-Fc-XP vector (43) using T4 Ligase (Promega) and transformed into E ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were then digested with LysC at 1ug/100ug protein (Promega) for 3 h at room temperature (RT ...
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bioRxiv - Plant Biology 2020Quote: ... Fusion proteins were purified using MagnetHis™ protein purification system (Promega) according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2024Quote: ... Proteins were precipitated through the addition of Protein Precipitation Solution (Promega) followed by vigorous vortexing ...
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bioRxiv - Biochemistry 2023Quote: ... Rat Gβ1 was fused with a His-tag at the N terminus and with a SmBiT subunit (peptide 86, Promega)34 after a 15-amino acid linker at its C terminus ...
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bioRxiv - Evolutionary Biology 2019Quote: ... bulked to 200ng using human genomic DNA (Promega). All samples were sheared for 150 seconds using a Covaris E220 (Duty Factor 5% ...
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bioRxiv - Systems Biology 2020Quote: The Human cell lysate was obtained commercially (Promega) and the yeast digest was prepared as previously described23 ...
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bioRxiv - Genomics 2019Quote: ... We added 4μl pooled commercial human gDNA (Promega) to all samples to ensure total gDNA > 1μg/35μl ...
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bioRxiv - Immunology 2021Quote: ... 3) goat anti-human H+L (Promega, W403B) at 1:3,000 dilution ...
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bioRxiv - Biochemistry 2020Quote: ... Human GSK-3β was purchased from Promega (V1991).
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bioRxiv - Genomics 2020Quote: ... and human gDNA (Promega, San Luis Obispo, CA) was added to reach a total input of 3 μg/130uL for fragmentation and library prep ...
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bioRxiv - Biochemistry 2021Quote: ... and SETD2 human ORF were procured from Promega. Deletion mutants of hnRNP L ...
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bioRxiv - Biochemistry 2022Quote: Human cancer cell line digest standards K562 (Promega) and diluted to 100 microgram/mL in 50mM TEAB and labeled with TMTPro reagents according to manufacturer instructions ...
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bioRxiv - Plant Biology 2020Quote: ... corniculatus plants was synthesized from 3 μg of total RNA using a Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV-RT) (Promega, WI, USA) and 100 pmol of random hexamers (Pharmacia Biotech) ...
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bioRxiv - Molecular Biology 2021Quote: cDNA synthesis for gene expression analyses was performed using 500 ng RNA using Moloney murine leukemia virus (M-MLV) reverse transcriptase and random hexamer oligonucleotide (both from Promega, Southampton, UK) using 500 ng RNA in a 25 µl reaction ...
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bioRxiv - Biochemistry 2020Quote: ... Moloney murine leukemia virus reverse transcriptase (MMLV-RT) enzyme and recombinant RNasin® ribonuclease inhibitor were purchased from Promega (Madison, WI, USA). Evagreen 2X qPCR MasterMix was purchased from Applied Biological Materials Inc ...
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bioRxiv - Cell Biology 2021Quote: ... and 1-3μl aliquots were taken for reverse transcription (RT) using the Moloney murine leukemia virus RT RNase H (−) (Promega, Madison, WI, USA), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... The resulting materials were used as template to generate cDNA using 100 U Moloney murine leukemia virus (MMLV) reverse transcriptase (Promega, Madison, WI), 0.45 μM N6 random hexamer primers (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: DNA was extracted from this concentrated virus sample using a modified protocol with the Wizard Genomic DNA Purification Kit A1120 (Promega, Madison, WI). Briefly ...
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bioRxiv - Microbiology 2019Quote: ... virus replication was quantified by measuring Renilla luciferase activity (relative light units [RLU]) using Bright-Glo™ Luciferase Assay System (Promega, USA) 48 hours post-reinfection ...
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bioRxiv - Microbiology 2019Quote: ... Virus infectivity was quantified by measuring Renilla luciferase activity (relative light units [RLU]) using Bright-Glo™ Luciferase Assay System (Promega, USA) on the Tecan microplate reader (Tecan Infinite® 200 Pro ...
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bioRxiv - Cell Biology 2020Quote: ... Total 500 ng RNA was used to synthesize cDNA by using Moloney-murine leukemia virus (M-MLV) reverse transcriptase (Promega, Madison, WI). Diluted cDNA was used in each quantitative reverse transcription-PCR (qRT-PCR) ...
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bioRxiv - Plant Biology 2022Quote: ... cDNAs were synthesized from 1.5 μg of total RNA with oligo(dT) primers using Moloney Murine Leukemia Virus Reverse Transcriptase MMLV-RT according to the manufacturer’s instructions (Promega, Madison, WI, USA). RT-qPCR was carried out in a Chromo4 system (Bio-Rad ...
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Mucociliary Clearance Augmenting Drugs Block SARS-Cov-2 Replication in Human Airway Epithelial CellsbioRxiv - Microbiology 2023Quote: ... The plates were incubated at 37°C under 5% CO2 for 144 hour and virus cytopathic effect was measured using the CellTiter-Glo® 2.0 Cell Viability Assay (Promega, Cat. # G9241) following the manufacturer instructions ...
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bioRxiv - Microbiology 2023Quote: ... the amount of input virus was normalized to the HiBiT value measured by Nano Glo HiBiT lytic detection system (Promega, Cat# N3040)] ...
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bioRxiv - Microbiology 2023Quote: ... Samples where a 1 log decrease in RLU compared to the no-serum virus-only control was observed were then selected for cytotoxicity assay using Cell Titre Glo kit (Promega, Southampton, UK) and light microscopy to verify viability of cells prior to undertaking PVNA (data not shown) ...
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bioRxiv - Microbiology 2024Quote: ... followed by reverse transcription to complementary DNA (cDNA) using 30 enzyme units(U) of the Moloney Murine Leukemia Virus reverse transcriptase (MMLV-RT) (Promega, WI, USA). The extension products were then analysed using the ABI3730xl DNA Analyzer (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... Mass Spec-Compatible Human Protein Extracts, Cat# V6951) and digested yeast protein extract (Mass Spec-Compatible Yeast Protein Extracts, Cat# V7461) were from Promega, commercial human plasma samples (Human Source Plasma ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins were purified by using MagneHis™ Protein Purification System (Promega, USA). The peptides of the transporters PDR6 ...
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bioRxiv - Biophysics 2022Quote: ... Fusion protein was purified using HisLink Protein purification resin (Promega (Madison, WI) #V8821 ...
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bioRxiv - Neuroscience 2021Quote: ... reverse mutant primer oIMR1437 5′-TCC ACC TAG CCT GCC TGT AC-3′) with 1U GoTaq polymerase (Promega, Madison, USA), 1X green GoTaq buffer ...
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bioRxiv - Biochemistry 2019Quote: ... Dimeric constructs are based on VY208 and were created by artificially dimerization through an N-terminal GST-tag (Reck-Peterson et al, 2006) and tagged with a HaloTag (Promega) at the C-terminus as well as a GFP at the very N-terminus ...
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bioRxiv - Developmental Biology 2020Quote: ... Nuclei were isolated by resuspending cell pellets in 200 μL Dig-wash buffer (CUT&Tag wash buffer supplemented with 0.05% digitonin (Promega, G9441) and 0.05% IGEPAL CA-630 (Sigma ...
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bioRxiv - Biochemistry 2020Quote: ... cDNAs were obtained from Origene in pCMV6 vector that also incorporates C-terminal Myc and FLAG tags (Myc-FLAG) or subcloned into a pHTC (Promega)-derived vector that expresses C-terminal HaloTag (41) ...
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bioRxiv - Cancer Biology 2021Quote: The 3’UTR of EIF3A (or mutants lacking U/TAG motif) were cloned downstream of the Renilla luciferase in the XhoI and NotI sites of psiCHECK2 vector (Promega, Madison ...
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bioRxiv - Microbiology 2022Quote: Templates for in vitro transcription of gene-specific 300-500 nt dsRNA and DENV2 EMSA probes were generated by PCR introducing a T7 promoter sequence or a universal tag at both 5’ and 3’ ends using the GoTaq Flexi DNA Polymerase (Promega). If present ...
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bioRxiv - Immunology 2020Quote: The strategy for genome-engineering to tag HLA-B with the HiBit peptide was developed based on a previous publication from Promega Corporation ...
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bioRxiv - Cell Biology 2020Quote: DRGs neurons infected with lentivirus expressing Halo-SFPQ were grown in XonaChip microfluidic chambers and were labeled with tetramethyl Rhodamine (TMR) Halo Tag ligand (Promega) according to the manufacturer’s instructions with modifications ...
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bioRxiv - Microbiology 2021Quote: ... where the multiple cloning site had been replaced by a synthetic construct (Genewiz) composed of a 5’ HiBiT tag (Promega), a new multiple cloning site and 3’ 3xHA tag ...
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bioRxiv - Bioengineering 2019Quote: ... after removal of its original signal peptide and was further fused to Halo-tag derived from HaloTag Control Vector (Promega) at the C-terminus ...
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bioRxiv - Biophysics 2019Quote: ... Cells were stained with SiR-dye (kindly provided by Kai Johnson, EFPL, Lausanne, Switzerland) shortly before imaging according to the Halo-tag protocol (Promega).
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bioRxiv - Cell Biology 2020Quote: ... Some cells at this stage were seeded onto coverslips and stable transfection of desired plasmids was confirmed by using specific fluorescent ligands to Halo-tag (TMR, Promega). The cells seeded in T75 flasks were allowed to grow for further 3-4 weeks in complete media with G418 replaced twice a week ...
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bioRxiv - Cell Biology 2021Quote: ... Eluted proteins from control HeLa cells with C-terminal mEGFP tag were digested with mass-spectrometry grade Lys-C and trypsin (Promega). Eluted proteins from a cell line lacking full-length Cdc20 (CDC20_M1-fs-M43 ...
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bioRxiv - Plant Biology 2024Quote: For in vitro pulldown experiments the coding region of AtMED25Δq was fused N-terminally with a flag-tag by PCR with oligonucleotides containing restriction site overhangs for cloning into pF3A WG BYDV (Promega). RAP2.12 C-terminally fused to CFP was previously established (Schmidt et al. ...