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Citations for Promega :
151 - 200 of 1628 citations for 6 Hydroxy 2 2 pyridinyl 4 3H pyrimidinone since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... 2 µL ROX (CRX reference dye, Promega, C5411) were added to 1 mL SybrGreen as a passive reference dye that allows fluorescent normalization for qPCR data ...
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bioRxiv - Microbiology 2023Quote: ... 2 µL of 100 bp DNA Ladder (Promega) was added to confirm amplicons size and run at 100 V for 45 mins ...
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bioRxiv - Molecular Biology 2023Quote: ... and treated with 2 μg trypsin (Promega, v511c), shaking overnight at 700 rpm ...
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bioRxiv - Molecular Biology 2023Quote: ... using the CellTiter-Blue assay (G8081/2, Promega) following the protocol of the manufacturer using an EnVision multimode plate reader (PerkinElmer) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 2 µL of sequencing grade modified trypsin (Promega) at a concentration of 0.1 µg/μL and 23 µL of 50mM ammonium bicarbonate/0.01% ProteaseMax were added ...
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bioRxiv - Biochemistry 2023Quote: Cell viability assays using CellTiter-Glo 2 (Promega) were performed according to the manufacturers’ instructions ...
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bioRxiv - Microbiology 2024Quote: ... Trypsin/LysC protease mix (2 µg/ µL, Promega) was added (1:25 protease ...
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bioRxiv - Molecular Biology 2020Quote: ... Cell numbers were quantified using a colorimetric 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS)-based CellTiter 96® AQueous One Solution Cell Proliferation Assay (Promega, Madison, WI) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... 5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay (Celltiter 96 Aqueous One-solution Cell Proliferation Assay, Promega, Madison, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... Digestion solutions were brought to a final concentration of 2 M urea and 2 mM CaCl2 and a second digestion was performed overnight at 37 °C using trypsin (Promega, v5280) at 1:100 w/w ...
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bioRxiv - Cell Biology 2021Quote: ... Protein material was reduced with tris(2-carboxyethyl) phosphine (TCEP; 10 mM final) and digested overnight with 2 μg sequence-grade modified trypsin Gold (Promega, V5280) in 50 mM ammonium bicarbonate (NH4HCO3 ...
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bioRxiv - Microbiology 2021Quote: ... rSARS-CoV-2/Δ7a-Nluc or rSARS-CoV-2/Nluc-2A infected cells was quantified using Nano-Glo® Luciferase Assay System (Promega) following the manufacturers’ specification.
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bioRxiv - Neuroscience 2023Quote: ... Membranes were rinsed 5 times for 8 min in PBS-tween 0.2% and incubated with secondary antibodies for 2 h at room temperature under agitation (HRP anti-mouse, 1:10000, Promega cat. # W4021). Membranes were further rinsed 5 times for 8 min in PBS-tween 0.2% ...
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bioRxiv - Molecular Biology 2023Quote: SMC5/6 hexamer purified from E.coli with a C-terminal HALO tag on Nse2 was incubated with a 2-fold molar excess of Janelia-Fluor646 HaloTag Ligand (Promega; 12 µM Smc5/6 hexamer + 24 µM label). After incubation for 1 h at 25°C ...
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bioRxiv - Developmental Biology 2021Quote: ... 2 mM dNTP mix and RNasin Ribonuclease Inhibitors (Promega). This mixture was incubated at 25 °C for 10 min ...
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bioRxiv - Cell Biology 2019Quote: ... alkylated and digested with 2 μg of Trypsin (Promega) overnight at 37°C ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were digested with 2 μg Trypsin (Promega V5280) for 20 hrs at 37°C ...
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bioRxiv - Bioengineering 2020Quote: ... and 2 U/μL RNasin Plus RNase Inhibitor (Promega). We performed DNase digestion at 37 °C for 1 hour followed by a 95 °C inactivation for 5 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... 2% (w/v) bisacrylamide (0.05% final concentration; V3141, Promega), 10× phosphate buffer saline (PBS ...
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bioRxiv - Cell Biology 2021Quote: ... 2) an RNAse inhibitor (1µL/mL of RNasin - Promega) was added to all the solutions after the fixation/permeabilization step ...
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bioRxiv - Biochemistry 2022Quote: ... and 2 μL T7 Express Enzyme Mix (Promega, P1320) and incubated at 37 °C for 4 hours unless otherwise indicated ...
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bioRxiv - Biophysics 2022Quote: ... and then digested with 2 μg of trypsin (Promega) overnight at 37°C ...
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bioRxiv - Physiology 2019Quote: ... 2 μL sequencing-grade trypsin (1 μg/μL; Promega) was added for the digestion at 37°C for 12 h ...
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bioRxiv - Neuroscience 2019Quote: ... 2 μg Sequencing Grade Modified Trypsin (Promega, Madison, WI) was added to each sample and digested overnight at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... comprised of 2% (v/v) of GloSensor reagent (Promega, #E1290 ...
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bioRxiv - Genomics 2020Quote: ... 2 mL of Wizard DNA Clean-Up resin (Promega) was added to the solution and mixed by inversion for two minutes ...
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bioRxiv - Biophysics 2020Quote: ... 2 x GoTaq®qPCR Master Mix (Promega, Switzerland) and 1 μl cDNA ...
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bioRxiv - Neuroscience 2022Quote: ... the sample was digested with trypsin (2 μg, Promega) in 50 mM ammonium bicarbonate (100 μl ...
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bioRxiv - Cancer Biology 2023Quote: ... (2) DNA template degradation by the DNaseI free (Promega), and (3 ...
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bioRxiv - Molecular Biology 2023Quote: ... using 2 µl of Fugene HD transfection reagent (Promega). The control reporter plasmid was transfected in parallel in equimolar amounts with pEGFP or pPRKRA ...
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bioRxiv - Genomics 2023Quote: ... 0.25 μl of 1% Digitonin in DMSO (Promega (2%), Cat ...
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bioRxiv - Synthetic Biology 2024Quote: ... 2 μL of 20-fold diluted furimazine stock (Promega) in live cell substrate (LCS ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 2 μL of CellTiter-Glo reagent (Promega, cat # G9683) was dispensed to each microplate well via BioRAPTR FRD ...
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bioRxiv - Cell Biology 2023Quote: ... 2 μL of ONE-glo luciferase detection reagent (Promega) was dispensed per well and luminance signal recorded with an Envision plate reader (Perkin Elmer).
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bioRxiv - Evolutionary Biology 2023Quote: ... 50 mM TEAB and 2 µg LysC+trypsin (Promega) were added ...
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bioRxiv - Microbiology 2024Quote: ... (2) addition of 600μL of Nuclei Lysis Buffer (Promega), and (3 ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were examined for viability every 4 to 6 weeks with the CellTiter-Glo assay (Promega). Cell viability was measured in quadruplicates by seeding the cells (2,000 to 3,000 per well in 96-well plate) ...
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bioRxiv - Cell Biology 2023Quote: ... then transfected with 4 μg pLZRS-GCaMP6 DNA plus 12 μl FuGENE 6 (Promega Cat. #E2691) in 800 μl of Opti-MEM (Thermo-Fisher Cat ...
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bioRxiv - Cell Biology 2021Quote: ... The samples were diluted with 20 mM HEPES pH 8.0 to a urea concentration of 2 M and the proteins were digested with 4 µl Trypsin/LysC (Promega V5073: 20ug + 80uL 50mM acetic acid) for 4 hours at 37°C and boosted with an extra 2µl Trypsin/LysC (Promega V5073 ...
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bioRxiv - Biochemistry 2019Quote: ... The samples were then diluted with 150 μL PBS and to this was added 1 mM CaCl2 (2 μL of a 200 mM stock in water) and trypsin (2 μg, Promega, sequencing grade, V5111) and the digestion was allowed to digest overnight at 37 °C with shaking ...
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bioRxiv - Neuroscience 2021Quote: ... 6 μl Fugene 6 (Promega, #E2691) and 90 μl DMEM medium (without FBS ...
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bioRxiv - Genetics 2024Quote: ... 6 µL of Fugene 6 (Promega), and OptiMem (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell viability was measured at day 4 and day 6 using the Cell-Titer Glo assay (Promega). Viability data was analyzed by comparing the relative viability change between base editing with the uORF gRNA and the associated parental CDS gRNA ...
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bioRxiv - Cell Biology 2021Quote: HEK293 T-REx SNAPf-GLP-1R-SmBiT cells were seeded in 12-well plates and co-transfected with 0.1 μg β-arrestin-2 fused at the N-terminus to LgBiT (LgBiT-β-arrestin-2; Promega, plasmid no. CS1603B118) and either 0.1 μg mini-Gs-Venus or mVenus supplemented with 0.8 μg pcDNA 3.1.
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bioRxiv - Molecular Biology 2023Quote: ... Figure 6 and Figure 7) to form condensates in cells was monitored by transient transfection of U-2 OS cells using FuGene (Promega; Figures 2 and 7) or electroporation (as before ...
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bioRxiv - Cell Biology 2020Quote: ... following manufacturer’s instructions using 200 ng psiCHECK™ 2 (Promega) as empty vector control or psiCHECK2 containing Tnf-α 3’ UTR or Il6 3’ UTR and 30 pmol miRNA scramble negative control ...
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bioRxiv - Molecular Biology 2019Quote: A commercial kit (HDAC-Glo™ 2 Assays, Promega, G9590) was used to measure HDAC2 activity ...
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A general role of zinc binding domain revealed by structures of σ28-dependent transcribing complexesbioRxiv - Molecular Biology 2020Quote: ... samples were treated with 2 U/mL DNase I (Promega) for 1 min at 37 °C ...
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bioRxiv - Biochemistry 2020Quote: ... After adding 2 μl of trypsin (0.5 μg/μl, Promega), peptide mixtures were incubated at 37°C for 4 hrs ...
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bioRxiv - Biochemistry 2020Quote: ... Before adding 2 μl of trypsin (0.05 μg/μl, Promega), 500 fmol of isotope-labeled peptides 1 and 2 were added to spike the ROS preparation ...