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Citations for Promega :
451 - 500 of 1628 citations for 6 Hydroxy 2 2 pyridinyl 4 3H pyrimidinone since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... BT-549 and HCC38 cells were transfected with both plasmids simultaneously (2 μg) using FuGene (Promega) according to the manufacturer’s instructions ...
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Genome modularization reveals overlapped gene topology is necessary for efficient viral reproductionbioRxiv - Synthetic Biology 2020Quote: ... 30 μg of protein was taken for digestion with trypsin (2% (w/w)) (Promega, United States) and left overnight at 37°C (∼18-hours) ...
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bioRxiv - Microbiology 2020Quote: ... incubated in the dark for 2 mins and read on a Glomax Multi+ Detection System (Promega).
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bioRxiv - Microbiology 2020Quote: ... 18h (SARS-CoV-2) or 24h (SARS-CoV) later by adding 1μM of Coelenterazine-H (Promega) at 1:400 dilution in PBS ...
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bioRxiv - Systems Biology 2021Quote: ... Both 1° and 2° assays were performed with 2x PCR master mix (Promega Corporation, Wisconsin, USA). The final PCR product from the 2° nested PCR was checked by electrophoresis in a 1.5% agarose gel ...
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bioRxiv - Neuroscience 2021Quote: ... Reverse transcription of 2 μg RNA was performed using M-MLV reverse transcriptase (Promega, WI, USA). The PCR-based amplification was done using synthesized-cDNA ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were transfected with 2 μg DNA using FuGENE® HD Transfection Reagent (REF E2311, Promega). After 24 hours ...
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bioRxiv - Biophysics 2020Quote: ... Cytotoxicity was measured using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) assay kit (Promega) by determining Formazan absorbance at 590 nm on a Tecan Safire automated plate reader ...
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bioRxiv - Microbiology 2020Quote: Supernatants were taken from cell medium and mixed with equal volume of 2 ×lysis buffer (Promega). Luciferase activity was measured with Renilla luciferase substrate (Promega ...
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bioRxiv - Microbiology 2020Quote: ... Fugene HD (Fugene HD [μL]:DNA [μg]:DMEM [μL] = 5:2:200;Promega, Madison, WI, USA) was used for transient transfection ...
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bioRxiv - Neuroscience 2021Quote: ... For RNA retrotranscription and RT-PCR it was used GoTaq 2-Step RT qPCR System (Promega) according to the manufacturer’s instructions and it was performed using Mic qPCR Cycler (BioMolecular Systems ...
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bioRxiv - Microbiology 2021Quote: Supernatants were taken from cell medium and mixed with equal volumes of 2×lysis buffer (Promega). Luciferase activity was measured with Renilla luciferase substrate (Promega ...
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bioRxiv - Immunology 2021Quote: ... Purified mAb (2 mg/mL in PBS) was treated with 1 unit of IdeS protease (Promega) per 1 µg of mAb ...
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bioRxiv - Biochemistry 2022Quote: ... by quickly mixing 2 μL of above refolding mixture with 50 μL of luciferase substrate (Promega). The activity of unheated luciferase in buffer B was set as 100%.
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bioRxiv - Immunology 2022Quote: ... mixed with Lumit BiT Set 2 (anti-mouse Ab-SmBiT, anti-rabbit Ab-LgBiT, Promega W1220) and added for 90min ...
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bioRxiv - Genetics 2022Quote: ... The lysate was digested overnight at 37 °C with 2 μg of sequencing-grade trypsin (Promega), after which the sample was acidified to 0.1% formic acid ...
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bioRxiv - Plant Biology 2022Quote: ... for 2 h as primary antibody and peroxidase-conjugated goat anti-rabbit antibody (1:10,000; Promega) for 1 h with 5 times 10 min washes in-between the incubations ...
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bioRxiv - Plant Biology 2023Quote: ... 2 μg of each purified plasmid was used as input in a 50 μl TnT (Promega) reaction incubated at 25 °C for 2 h ...
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bioRxiv - Physiology 2023Quote: 2 μg RNA was converted to first–strand cDNA by using reverse transcriptase (Promega, Madison, WI) and Oligo–D (T ...
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bioRxiv - Developmental Biology 2023Quote: ... PCR amplification of exon 2 of the NR2F2 gene was performed using PCR Master Mix (Promega; primer pair is listed in Appendix Table S1) ...
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bioRxiv - Plant Biology 2023Quote: ... cDNA was synthesized (using 2 µg RNA) using the Go Script™ Reverse Transcriptase Kit (Promega) following the manufacturer’s instruction ...
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bioRxiv - Systems Biology 2023Quote: ... and digested overnight at 37°C with a 2 μg Trypsin/Lys-C enzyme mix (Promega). The next day ...
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bioRxiv - Molecular Biology 2023Quote: The pSV-Rluc renilla luciferase internal standard vector was derived from psiCHECK-2 (Promega, catalog # C8021) by digestion with EagI to remove the firefly luciferase gene ...
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bioRxiv - Plant Biology 2023Quote: ... LUC images were taken 2-3 days after infiltration by applying 1 mM Beetle luciferin (Promega) on adaxial leaf surface.
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bioRxiv - Cell Biology 2023Quote: ... The cells were successively lysed and assayed by a 2-DG uptake assay kit (J1341, Promega) according to the instruction ...
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bioRxiv - Biochemistry 2023Quote: ... 0.02 µg of plasmid and 2 µg of carrier DNA were combined with FuGENE HD (Promega) at a 3:1 ratio and added per well of a 6-well plate seeded with 70% confluent HEK293 cells ...
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bioRxiv - Microbiology 2023Quote: ... described in Supplemental Table 2 were used for amplification with GoTaq G2 polymerase (Promega, WI, USA). Amplification was performed by 35 cycles PCR each consisting of 94°C for 30 sec ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated in a medium containing 2 nM Janelia Fluor 646 (JF646) HaloTag ligand (Promega) and 0.5 μg/mL Hoechst33342 (Nacalai Tesque ...
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bioRxiv - Physiology 2019Quote: ... Plasmid DNA (6 µg) was mixed (1 : 3 ratio) with transfection reagent (Fugene 6; Promega, UK) in reduced serum media (OptiMEM ...
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bioRxiv - Biochemistry 2022Quote: ... were transfected using FuGene 6 (Promega). The supernatant containing V0 virus (virus passage zero (progeny virus) ...
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bioRxiv - Immunology 2022Quote: ... using Fugene 6 transfection reagent (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... using FuGene 6 transfection reagent (Promega). The medium was changed 18 h after transfection ...
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bioRxiv - Cell Biology 2021Quote: ... and 1.2 µl Fugene 6 (Promega) transfection mix and incubated for 15min at room temperature before adding to HeLa cells plated in one well of a 24 well plate ...
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bioRxiv - Cell Biology 2021Quote: ... FuGENE 6 reagent (Promega, Madison, WI) was used for transient transfection of HeLa cells according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... S7A–C using FuGene 6 (Promega). Two days after transfection ...
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bioRxiv - Molecular Biology 2019Quote: ... 8μL of Fugene-6 (Promega, E2691) was mixed with various amounts of plasmid DNA and allowed to incubate at room temperature for 15 minutes before addition to cells ...
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bioRxiv - Cell Biology 2021Quote: ... FuGENE® 6 Transfection Reagent (Promega); Luminata Forte Western HRP substrate (Millipore) ...
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bioRxiv - Microbiology 2019Quote: ... using Fugene 6 transfection reagent (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... MDCK) or with FuGENE 6 (ProMega) (HeLa) ...
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bioRxiv - Cancer Biology 2021Quote: ... using FuGENE 6 Transfection Reagent (Promega) according to manufacturer protocol ...
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bioRxiv - Genomics 2023Quote: ... and transfected (Fugene 6, Promega # E269A) with pBABE-puro SV40 LT plasmid (Addgene # 13970 ...
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bioRxiv - Immunology 2023Quote: ... using Fugene 6 transfection reagent (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... N-glycans were released from the membrane-bound protein using 2 U PNGase F (Promega, WI, USA) with overnight incubation at 37°C ...
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bioRxiv - Plant Biology 2019Quote: ... using 1 - 2 µg of total RNA and 250 ng of a mixture of random hexanucleotides (Promega) and incubated for 50 min at 50°C ...
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bioRxiv - Genetics 2020Quote: ... 2 µg of DNA in 50 µl of DMEM and 50 µl of FuGene6 transfection reagent (Promega) at 1mg·ml-1 in DMEM were incubated separately for 5 min at room temperature ...
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bioRxiv - Biophysics 2021Quote: ... the cells were transfected with 1 – 2 μg EphA2-mTurquoise plasmid DNA using FuGene HD (Promega, #E2311) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... The results were calculated using the 2-△△CT method according to the GoTaq qPCR Master Mix (Promega) manufacturer’s specifications.
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bioRxiv - Plant Biology 2020Quote: ... genomic DNA was extracted from 2-wk old seedlings using the Wizard Genomic DNA purification kit (Promega) and was subjected to PCR analysis using two AtNIP2;1 gene specific primers and the left border T-DNA primer (all primers used in this study are described in Supplemental Table 1) ...
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bioRxiv - Plant Biology 2021Quote: ... Selection was done twice for 2 weeks on medium containing 25 µg/ml G418 (Promega, Mannheim, Germany) starting 2 weeks after transformation with a 2-weeks release period in between ...
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bioRxiv - Developmental Biology 2022Quote: ... The results were calculated using the 2-ΔΔCT method according to the GoTaq qPCR Master Mix (Promega) manufacturer’s specifications.