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Citations for Promega :
301 - 350 of 6684 citations for 20 Hydroxyecdysone ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... lysed with 1X Passive Lysis Buffer then analysed using the Dual-Luciferase Assay kit on a GloMax multi+ plate reader (Promega, Southampton, UK).
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bioRxiv - Microbiology 2020Quote: ... for 20 min at 56°C before DNA was purified with Maxwell® RSC Blood DNA Kit (Promega Pte. Ltd., Cat. No. AS1400). DNA concentration was quantified using Qubit® 2.0 fluorometer ...
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bioRxiv - Immunology 2022Quote: Caspase-1 activity was quantified using a Caspase-Glo 1 Inflammasome Assay kit (Promega, Madison, WI). THP-1 cells were treated and harvested as described above ...
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bioRxiv - Immunology 2019Quote: ... and the pellet was resuspended in 20 mL buffer A (NaPO4 20 mM, NaCl 500 mM and imidazole 20 mM, pH 7,4) containing 5 U of DNAse (Promega) and 30 ug/mL lysozyme (Sigma ...
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bioRxiv - Molecular Biology 2022Quote: ... The luciferase bioluminescence images were captured by the dual-luciferase reporter assay system (GloMax 20/20 Luminometer; Promega, USA). For Y2H ...
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bioRxiv - Microbiology 2021Quote: ... and light emission was measured in arbitrary light units on a Glomax 20/20 Luminometer (Promega, Madison WI, USA). The luciferase assays were performed as suggested by the manufacturer’s protocols.
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bioRxiv - Bioengineering 2024Quote: ... Protein A biosensors (GatorBio) were equilibrated for 20 min in BLI buffer (PBS buffer supplemented with 0.02% tween-20 (Promega) and 0.1% bovine serum albumin (BSA ...
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bioRxiv - Molecular Biology 2024Quote: ... The luciferase activities were measured using the Dual-Glo Luciferase Assay System and a GloMax 20/20 luminometer (Promega). The intensity of Fluc activity was normalized to the intensity of Rluc activity ...
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bioRxiv - Cell Biology 2019Quote: ... ELISA data was normalized to viable cell number determined by MTT assay (Boehringer Ingelheim) or CellTiter-Blue (Promega). IFN levels were determined using a HEK293T IFN reporter cell line (clone 3C11 ...
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bioRxiv - Neuroscience 2020Quote: ... 1 μg RNA was reverse transcribed using Reverse Transcription Kit (Promega) with oligo(dT ...
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bioRxiv - Developmental Biology 2020Quote: ... neural stem / progenitor cells were seeded in 96-well coated plates (1×104 cells per well) and RealTime-GloTM MT Cell Viability Assay (Promega) was used with the continuous-read protocol at 0 ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated 1:1 for 5 minutes in the dark in opaque 96 well plates with NanoGlo Substrate freshly diluted 1:50 in NanoGlo Buffer (Promega). Luminescence was measured on a GloMax 96 Microplate Luminometer.
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bioRxiv - Molecular Biology 2019Quote: ... WT and m6A-mut expressing cells in 24-well plates were infected with the indicated virus (MOI 1) and dual luciferase assay (Promega) was performed at 48 hours post-infection according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... The cells were diluted and aliquoted to 96-well plates to make less than 1 cell count per well and lysed by 30 μl of Passive Lysis Buffer (Promega) when confluent ...
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bioRxiv - Molecular Biology 2020Quote: ... Plates were incubated at room temperature for 1 hour before luminescence was measured using a GloMAX Discover Plate reader luminometer (Promega). Three replicate experiments were performed with three biological replicates for each cell type and treatment within each experiment.
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bioRxiv - Immunology 2020Quote: ... the cells were washed with PBS and then lysates were prepared by scraping the cells from plates in the presence of 1× Passive lysis buffer (Promega). Luciferase assays were performed according to Dual-Luciferase Assay System (Promega ...
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bioRxiv - Immunology 2021Quote: ... Plates were incubated at 25 °C for 1 hr and fluorescence was measured using a BioTek Synergy Neo plate reader (Promega). The terbium (Tb ...
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bioRxiv - Immunology 2021Quote: ... the plates were washed with PBS and the cells were lysed with 1× reporter lysis buffer (100 µl per well; Promega). Following a freeze-thaw cycle ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 40,000 cells per well were seeded into poly-D-lysine-coated 96-well plates (Brand, 781965) and the Halo-ligand was added (1:2,000; Promega, G980A). For each transfection ...
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bioRxiv - Cancer Biology 2023Quote: Cells were plated at a density of 1*10^6 per 10cm plate and transfected on the following day using Fugene HD (Promega, Madison ...
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bioRxiv - Bioengineering 2023Quote: ... 2000 transfected cells were replated into white 384-well tissue culture plates in the presence or absence of 1 μM HaloTag NanoBRET-618 Ligand (Promega) and incubated overnight at 37 °C ...
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bioRxiv - Genetics 2019Quote: ... cDNA was synthesised using 1 µg of total RNA in a 20 µL final volume using the GoScript Reverse Transcription System (Promega, USA) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... The protein pellet was resuspended in 190 ul of 50 mM ammonium bicarbonate and digested with 20 ul of 1 ug/ul trypsin gold (Promega V5280) at 37°C for 4 hours.
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bioRxiv - Biochemistry 2021Quote: ... and the “on-bead-digestion” plate (100 μl 20 mM Tris pH 8.5, Sigma-Aldrich 10708976001, 1 μg/mL LysC, Wako 129-02541, 2 μg/mL Trypsin, Promega V5111). The programmed sequence is ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Amplification reactions were run in a 20 μL volume using the GoTaq 1-Step RT-qPCR System (Promega, Madison WI, USA) and the recommended manufacturer’s instructions as described in Cervera et al ...
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bioRxiv - Biochemistry 2023Quote: ... samples were resuspended in 6 M guanidine hydrochloride.250 µg of protein were digested overnight at 37 °C using trypsin with a 1:20 trypsin to sample ratio (Promega, #V5117). The next day ...
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bioRxiv - Bioengineering 2024Quote: ... were taken for overnight digestion with mass spec grade Trypsin/Lys-C Mix (Promega, Madison, WI, cat. No. V5071, 1:20). Samples were reduced with TCEP (final concentration 5 mM ...
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bioRxiv - Molecular Biology 2022Quote: ... the transfected cells plated in 96-well black plates were examined using a CellTiter-Blue Cell Viability Assay kit (Promega, Madison, WI, USA). Briefly ...
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bioRxiv - Synthetic Biology 2021Quote: ... 200µL (for 48-well plate format) or 400µL (for 24-well plate format) complete DMEM with or without 10µM furimazine (Promega). Cells were incubated at 37°C under 5% CO2 either in the dark ...
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bioRxiv - Molecular Biology 2021Quote: Reverse transcription was performed with 1-4 µg of total RNA in a 20 µl reaction volume with 10 µM VNN-(dT)20 primer and the GoScript Reverse Transcriptase (Promega). For the semi-quantitative end-point PCRs the MyTaq Red Mix (Bioline ...
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bioRxiv - Microbiology 2019Quote: ... vortexed for 5 s and then immediately measured for luminescence (expressed as relative light units [RLU]) using a GloMax 20/20 Luminometer (Promega). All strains were tested in quadruplicate from three independent cultures.
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bioRxiv - Cell Biology 2021Quote: ... luciferase activity was measured at luminometer Glomax 20/20 using Dual-Luciferase Reporter Assay System (cat. #E1910-Promega, Fitchburg, USA) following manufacturer's instructions ...
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bioRxiv - Biochemistry 2022Quote: ... Infected cells were harvested at 18 hpi and luciferase activity was measured using a luminometer-Glomax 20/20 (Promega, USA).
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bioRxiv - Microbiology 2021Quote: ... Cells were lysed and luciferase activity was assessed using the Luciferase Assay System and the Glomax 20/20 luminometer (Promega). Infectivity values were analyzed and plotted using Prism 8.
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bioRxiv - Microbiology 2022Quote: ... used as PCR template and directly for luminescence quantification according to the manufacturer’s instructions (20 μL phage plus 20 μL substrate, Nano-Glo® Luciferase Assay System, Promega).
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bioRxiv - Plant Biology 2023Quote: ... and the ratio of Firefly luciferase to Renilla luciferase was measured and calculated by using a luminometer (GloMax 20/20, Promega), with a Dual-Luciferase Reporter Assay System (E1910 ...
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bioRxiv - Molecular Biology 2022Quote: ... FLuc and RLuc activities in lysates were sequentially measured by using Dual-Glo luciferase assay reagents and a GloMax 20/20 luminometer (Promega) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: ... and luciferase activity was measured after 48 h using the dual-luciferase reporter assay system and GloMax 20/20 (Promega). pRL-null Renilla plasmid was cotransfected at 0.4 pg/μL to control for transfection efficiency ...
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Engineering synthetic CopT/A-based genetic circuits for miRNA imaging and functional gene regulationbioRxiv - Synthetic Biology 2023Quote: ... 50 μL of coelenterazine substrate was added to the wells and Gluc activity was measured using a Glomax-20/20 luminometer (Promega).
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bioRxiv - Immunology 2022Quote: ... the luciferase activity in cells was analyzed using a GloMax 20/20 luminometer with the Dual-Luciferase Reporter Assay system (Promega).
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bioRxiv - Microbiology 2023Quote: ... Mtb samples were inactivated at >95°C for 20 min and stored at -20°C until analyzing the ATP levels using the BacTiter Glo assay (Promega) as previously described (19) ...
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bioRxiv - Cell Biology 2024Quote: ... Reporter activity was analyzed 48 h post-transfection using the Dual luciferase reporter assay system and GloMax 20/20 Luminometer (Promega) instrument ...
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bioRxiv - Molecular Biology 2021Quote: ... on a GloMax Explorer plate reader (Promega). Drug treatments were performed by incubating the reaction with the indicated compound for 15 minutes at 30 degrees C prior to the addition of RNA.
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bioRxiv - Developmental Biology 2020Quote: ... on a GloMax-Multi plate reader (Promega). Resulting luciferase activities were normalized to the geometric mean of Pkm and Cnot10 5’UTR activities and then to DMSO treated cells.
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bioRxiv - Developmental Biology 2020Quote: ... on a GloMax-Multi plate reader (Promega). Resulting luciferase activities were first normalized to the geometric mean of Pkm and Cnot10 5’UTR activities and then to wildtype micromass cultures.
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bioRxiv - Developmental Biology 2020Quote: ... on a GloMax-Multi plate reader (Promega).
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bioRxiv - Molecular Biology 2020Quote: ... on a GloMax Explorer plate reader (Promega).
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bioRxiv - Molecular Biology 2022Quote: ... by using the GloMax plate reader (Promega).
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bioRxiv - Genetics 2023Quote: ... on the GloMax Discover plate reader (Promega). β-Catenin induced BAR:Luciferase signal was normalized by Renilla luminescence from the constitutively active Tk promoter.
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bioRxiv - Bioengineering 2023Quote: ... and a GloMax explorer plate reader (Promega) following the manufacturer instructions ...