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Citations for Promega :
4801 - 4850 of 5642 citations for ssc mir 376a 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... for 1 h at 37 °C and cDNA was then synthesized using a Moloney murine leukemia virus (MMLV) reverse transcription kit with random primers following the manufactureŕs protocol (Promega). To check for the presence of remaining DNA ...
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bioRxiv - Bioengineering 2022Quote: ... intracellular levels of unesterified cholesterol and total cholesterol were separately assayed using the Amplex Red Cholesterol Assay Kit (Promega), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and the levels of firefly luciferase activity determined using a luciferase assay kit according to manufacturer’s instructions (Promega; E1500). To quantify background (i.e. ...
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bioRxiv - Genomics 2020Quote: DNA was extracted from a single colony of each isolate with a Wizard® Genomic DNA Purification Kit (Promega), and the quantity and quality were determined with a Quantus Fluorometer (Promega ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were lysed 48-hrs later for FLuc quantitation using a Luciferase 1000 Assay System kit (Promega Cat#E4550) according to manufacturer’s instructions and read on a Veritas luminometer ...
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bioRxiv - Molecular Biology 2020Quote: ... cDNA was synthesized from 400 ng of RNA using ImProm-II Reverse Transcription system kit and random primers (Promega). Quantitative PCR was performed in technical duplicates using SensiMix SYBR mix (Bioline) ...
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bioRxiv - Cell Biology 2021Quote: ... and a total amount of 2 μg RNA was reverse transcribed with a GO Script Reverse Transcriptase kit (Promega) using random primers ...
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bioRxiv - Cell Biology 2021Quote: Intracellular levels of ATP in male and female PTECs were measured with a CellTiter-Glo 2.0 Assay Kit (Promega), following manufacturer instructions (see Supplemental Methods).
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bioRxiv - Microbiology 2020Quote: ... Genomic DNA of all the strains was isolated using the Wizard genomic DNA purification kit (Promega, Madison, WI, USA), following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: The activity of caspase-3/7 was measured by Caspase-Glo-3/7 assay kit according to the manufacturer’s instructions (Promega). Cell death was assessed by an Annexin-V FITC binding assay (Miltenyi ...
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bioRxiv - Bioengineering 2020Quote: ... and an absorbance LDH assay (Lactate dehydrogenase assay, CytoTox-ONE™ Homogeneous Membrane Integrity Assay kit from Promega, G7890) was used to determine the level of cytotoxicity caused by membrane compromisation ...
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bioRxiv - Molecular Biology 2019Quote: ... DNA was then extracted using 100μl of the homogenate according to the Wizard genomic DNA purification kit (Promega, France) following the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2019Quote: ... A total of 500 ng RNA was reverse-transcribed to cDNA using the Reverse Transcription Kit (Promega, Madison, USA), according to the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2019Quote: Cell growth was assessed using Proliferation Assay™ Kit in accordance with the manufacturer’s instructions (Promega, Madison, WI, USA) [15 ...
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bioRxiv - Plant Biology 2019Quote: ... frozen and ground in liquid nitrogen and resuspended in 1x passive lysis buffer (Dual Luciferase Assay kit, E1910, Promega), using 2 volumes/mg of material ...
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bioRxiv - Developmental Biology 2019Quote: ... and 1 μg of RNA was reverse-transcribed by the oligo (dT) primer using a reverse transcription kit (Promega) after digestion with RNase-free DNaseI (Fermentas) ...
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bioRxiv - Pathology 2020Quote: ... All reactions were performed as biological triplicates and technical duplicates using a GoTaq® qPCR Master Mix Kit (Promega). The primers were designed according to the ARG gene sequence (LinJ.35.1490 ...
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bioRxiv - Neuroscience 2019Quote: ... Genomic DNA was isolated from tail snip samples with Maxwell® Mouse Tail DNA Purification Kit (Promega, Madison, WI). Polymerase chain reaction (PCR ...
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bioRxiv - Molecular Biology 2020Quote: ... Hygromycin (150ug/ml) was used to select for cells with integration and Nano-Glo Dual-luciferase assay kit (Promega) was used to confirm expression of both Firefly and Nano-luciferase in clonally isolated cells.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and were frequently tested for absence of mycoplasma and cell lines were authenticated using STR profiling kit from Promega.
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bioRxiv - Zoology 2020Quote: ... Other sections were digested in a mixture of 97% Nuclei Lysis Solution (Promega; from the Wizard DNA isolation kit) and 3% 20mg/ml Proteinase K (Promega) ...
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bioRxiv - Molecular Biology 2020Quote: gDNA samples for library preparation were extracted from overnight LB cultures using the Wizard Genomic DNA Purification kit (Promega) as described above ...
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bioRxiv - Molecular Biology 2020Quote: ... and 0.5 ml (approximately 2 × 109 cells) were used for gDNA extraction with the Wizard Genomic DNA Purification kit (Promega), which typically yielded 50 μl of 0.5-1.5 μg/μl gDNA.
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bioRxiv - Molecular Biology 2021Quote: ... Reactions were stopped by addition of TFA to 0.1% and the production of SAH was measured using the MTase-Glo Methyltransferase Assay kit (Promega) according to the manufacturer’s instructions using a Hidex Sense microplate reader..
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bioRxiv - Microbiology 2019Quote: Genomic DNA (gDNA) was extracted from pure cultures grown overnight using the Wizard® Genomic DNA purification kit (Promega) following the manufacturer’s protocol for Gram-negative bacteria.
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bioRxiv - Microbiology 2021Quote: ... Cell viability was determined 24 h after the addition of compound-containing DMEM using the CellTiter-Glo kit (Promega) and a luminescence plate reader (Perkin Elmer) ...
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bioRxiv - Cancer Biology 2020Quote: ... Forty-eight hours post transfection (or twenty-four hours for promoter truncation/mutation) dual-luciferase reporter assay kit (Promega) was used to analyze bioluminescence on SpectraMax i3x microplate reader (Molecular Devices ...
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bioRxiv - Microbiology 2021Quote: ... Luciferase activity in cell lysates were measured by using either Firefly or Renilla luciferase kits (Promega, Madison WI, USA), and light emission was measured in arbitrary light units on a Glomax 20/20 Luminometer (Promega ...
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bioRxiv - Microbiology 2019Quote: ... genomic DNA was extracted from frozen samples by using the Wizard Genomics DNA purification kit (Promega, Madison, WI, USA). One microliter of purified DNA was analysed by gel electrophoresis to check for integrity ...
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bioRxiv - Molecular Biology 2020Quote: ... ZmIBH1-1 was fused to HaloTag using the kit from pFN19K HaloTag T7 SP6 Flexi Vecto (cat#G184A) (Promega). ZmCLA4 fused to HaloTag was expressed using the TnT SP6 High-Yield Wheat Germ Protein Expression System (L3260 ...
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bioRxiv - Cell Biology 2021Quote: ... Supersignal kit (Pierce) and a LAS-3000 imager (Fujifilm) or AP-coupled antibodies and NBT/BCIP as substrates (Promega). Rabbit polyclonal antibodies obtained and purified using full-length Arpin were previously described (Dang et al ...
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bioRxiv - Microbiology 2020Quote: ... fLuc activity was measured using a luciferase assay system kit and GloMax® Navigator Microplate Luminometer (both from Promega).
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bioRxiv - Physiology 2020Quote: ... Blocked membranes were incubated for 2 hours with DNA probe synthesized using a Prime-a-Gene® kit (Promega) and washed in mild and stringent Southern wash buffers ...
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bioRxiv - Immunology 2021Quote: ... and several sample types were evaluated for ADCC and ADCP functionalities using the corresponding Reporter Bioassay kit (Promega Corporation). ADCC and ADCP induction was expressed as induction ratio (IR) ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were harvested 48 hours post-transfection and genomic DNA was extracted using the Wizard® SV Kit (Promega). Genomic targeting efficiency for each gRNA was determined through annealing and digestion with T7 Endonuclease I (NEB ...
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bioRxiv - Biochemistry 2021Quote: Caspase-3/7 activity was quantified by fluorometric assay using ApoONE Homogeneous Caspase-3/7 Assay Kit (Promega, US). AU565 cells ...
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bioRxiv - Microbiology 2021Quote: ... seriolae gDNA was extracted from 5-day old cultures using the Wizard® Genomic DNA Purification Kit (Promega, USA) as per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Relative cell viability was determined using a Cell counting kit-8 (Dojindo) and read on a microplate reader (Promega).
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bioRxiv - Molecular Biology 2021Quote: ... 3βHSD and FSHR in the supernatant of cultured GCs were quantified using GoTaq® qPCR Master Mix Kit (Promega) as previously described [29] ...
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bioRxiv - Evolutionary Biology 2020Quote: ... RNA was extracted from clinical samples (300μl) via a Maxwell 48 device using the Maxwell RSC Viral TNA kit (Promega) with a viral inactivation step using Proteinase K ...
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bioRxiv - Immunology 2020Quote: ... The identity of the Huh7 cells used in this study was verified by using the GenePrint STR kit (Promega) (DNA Analysis Facility ...
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bioRxiv - Developmental Biology 2021Quote: ... Total RNA was isolated from whole mount larvae or dissected tissue using the Maxwell RSC simplyRNA Tissue kit (Promega Corp. ...
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bioRxiv - Genomics 2021Quote: ... We extracted the total RNA of the larval stage using ReliaPrep RNA Tissue Miniprep kit (Promega, Madison, WI/USA) according to the supplier’s recommended protocol ...
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bioRxiv - Biochemistry 2020Quote: The microbial viability was assessed in equalized bacterial cultures using the BacTiter-GloTM Microbial Cell Viability Assay kit (Promega) according to the manufacturer’s recommendations and luminescence was measured using a VarioSkan plate reader (Thermo Scientific).
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bioRxiv - Immunology 2020Quote: ... The culture supernatants were collected and analysed for lactate dehydrogenase (LDH) by a commercially available CytoTox 96 Non-radioactive Cytotoxicity Assay kit according to the manufacturer’s (Promega) instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... The identity of the Huh7 cells used in this study was verified by using the GenePrint STR kit (Promega) (DNA Analysis Facility ...
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted from filter paper punches using the manufacturer’s protocol for Promega Maxwell DNA IQ Casework Sample kit (Promega) or Qiagen DNA Blood kit (Qiagen) ...
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Activation of innate immune cGAS-STING pathway contributes to Alzheimer’s pathogenesis in 5×FAD micebioRxiv - Neuroscience 2022Quote: ... cDNA was generated from 1 μg total RNA per sample using the M-MLV Reverse Transcriptase kit (M1705, Promega). To detect RNA transcript of targeted genes ...
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bioRxiv - Cancer Biology 2022Quote: ... The anti-proliferative effects of these compounds were assessed using the Cell Titer Glo assay kit (Promega Cat. # G7570) following manufacturer protocol ...
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bioRxiv - Biophysics 2022Quote: ... Cells were stimulated on the next day and lysed after 24 hrs following manufacturer’s protocol in Dual Luciferase Assay kit (Promega). Luminance signal readings were performed using or SpectraMax i3x (Molecular Devices) ...