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Citations for Promega :
4601 - 4650 of 5642 citations for ssc mir 376a 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Zoology 2021Quote: ... Sections were digested in a mixture of 97% Nuclei Lysis Solution (Promega; from the Wizard DNA isolation kit) and 3% 20mg/ml Proteinase K (Promega) ...
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bioRxiv - Neuroscience 2020Quote: DNA of tissue samples was extracted and purified using The Wizard Genomic DNA Purification Kit (Promega, Madison, WI). Total DNA was quantified using the Life Technologies Qubit 2.0 Fluorometer (ThermoFisher ...
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bioRxiv - Microbiology 2020Quote: RNA (400 ng) was reverse transcribed into cDNA using random oligos and the GoScript Reverse Transcriptase kit (Promega). The cDNA was probed using primers specific to target genes in the StepOnePlus Real Time PCR system as described above ...
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bioRxiv - Systems Biology 2021Quote: Genomic DNA from all screens was extracted from cell pellets using the Wizard Genomic DNA Purification kit (Promega). Sequencing libraries were prepared from 100 μg of gDNA for control sample and non-cytopathic screen samples or 10 μg of gDNA for cytopathic screen samples via a 2-step nested PCR using primers that include Illumina TruSeq adapters with i5 and i7 indices ...
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bioRxiv - Microbiology 2020Quote: Genomic DNA from Xf strain CFBP 8070 was extracted with the Wizard genomic DNA Purification Kit (Promega, France) and used to prepare a 10-fold serial dilutions from 220 ng.mL−1 (corresponding to 0.8×108 copies.mL−1 of genomic DNA ...
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bioRxiv - Microbiology 2021Quote: ... Total RNA was extracted from the frozen samples using the SV Total RNA Isolation kit (Promega, Beijing, China), and cDNA was synthesized using the PrimeScript RT reagent Kit with gDNA Eraser (Takara ...
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bioRxiv - Microbiology 2021Quote: Viral RNA was extracted from all samples using the Maxwell RSC Viral Total Nucleic Acid Purification Kit (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... viral RNA was extracted from all samples using the Maxwell RSC Viral Total Nucleic Acid Purification Kit (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... 2 ug of RNA were reverse transcribed using a reverse transcription kit (GoScript™ Reverse Transcription System, Promega). The abundance of specific transcript was measured by probing 1 μL cDNA by quantitative real-time PCR in a total volume of 10 µl containg 5 μL SYBR-green master mix (Roche) ...
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bioRxiv - Microbiology 2021Quote: ... DNA was extracted from filters or 1mg of oyster tissues using the Wizard Genomic DNA extraction kit (Promega). All amplification reactions were analysed using a Roche LightCycler 480 Real-Time thermocycler (Genomic platform SBR) ...
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bioRxiv - Microbiology 2020Quote: ... intracellular ATP was quantified by using the BacTiter-Glo Microbial Cell Viability Assay Kit (Promega CAT No.: G8230). Aliquots of 100 µl of bacterial culture were collected at various time points and mixed with an equal volume of the BacTiter-Glo reagent and incubated for 5 min in the dark ...
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bioRxiv - Cancer Biology 2020Quote: ... One volume was used for extraction of chromosomal DNA using the standard Wizard genomic DNA prep kit (Promega) protocol (CRC2631) ...
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bioRxiv - Neuroscience 2020Quote: ... The impact of compounds on H4 cell toxicity was assayed using the CytoTox-Glo kit (Promega, Madison, WI).
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bioRxiv - Neuroscience 2020Quote: ... The impact of compounds on H4 cell toxicity was assayed using the CytoTox-Glo kit (Promega, Madison, WI).
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bioRxiv - Cell Biology 2021Quote: ... Proteasome activities (chymotrypsin-like, trypsin-like and caspase-like) were assayed using the Proteasome-Glo kit (Promega, USA) in 384-well plates for luminescent assays ...
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Mitochondrial and metabolic remodeling in human skin fibroblasts in response to glucose availabilitybioRxiv - Cell Biology 2021Quote: ... intracellular ATP levels were measured using CellTiter-Glo® Luminescent Cell Viability Assay Kit (Promega, Madison, WI, USA), at room temperature and according to the vendor’s recommendations ...
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bioRxiv - Cell Biology 2021Quote: Total RNA was prepared from cultured cells using the ReliaPrep™ RNA Cell Miniprep System kit from Promega according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ATPase activity of the purified proteins was determined using two kits – Enliten ATPase assay (FF2000, Promega, Moscow, Russia) and malachite green phosphate assay (MAK307 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were extracted and the NADPH/NADP+ ratio was measured using the NADP/NADPH-Glo Assay kit (Promega) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: TUNEL Cell death assays were performed following the instructions of DeadEnd™ Fluorometric TUNEL System kit (Promega, G3250). Briefly ...
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bioRxiv - Biochemistry 2022Quote: ... Reaction progress was measured as a function of SAM to SAH conversion using the MTase Glo Kit (Promega). KM values for SAM were determined by the same method but holding substrate concentrations constant at 100 μM and varying the concentration of SAM ...
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bioRxiv - Microbiology 2022Quote: Cytotoxicity was measured using the Promega CytoTox 96 Nonradioactive Cytotoxicity Assay kit (cat # G1780, Promega, Madison, WI, USA) in 50 μL reactions and 96-well format (cat # 161093 ...
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bioRxiv - Immunology 2022Quote: Total DNA was isolated from cardiac tissue using the Kit Wizard® Genomic DNA purification (Promega Madison WI). Each sample was quantified employing a BioSpec-nano spectrophotometer system (SHIMADZU® ...
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bioRxiv - Microbiology 2022Quote: ... the level of LDH in the basal culture medium was quantified by LDH-Glo Cytotoxicity Assay kit (Promega).
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bioRxiv - Plant Biology 2022Quote: ... the protoplasts were harvested and the PHT1;1 promoter activity was quantified with a dual-luciferase assay kit (Promega).
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bioRxiv - Immunology 2022Quote: DNA was extracted from snap frozen intestinal tissue samples with the Wizard® Genomic DNA Purification Kit (Promega) per manufacturer’s instructions for animal tissue.
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bioRxiv - Microbiology 2022Quote: Total RNA was extracted from 20–30 mg of liver tissue with the Maxwell SimplyRNA Tissue Kit (Promega) on a Maxwell RSC 16 instrument (Promega ...
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bioRxiv - Bioengineering 2022Quote: ... Uptake of 2-deoxyglucose by the adipocytes was measured using a Glucose Uptake-Glo™ Assay kit (Promega) and luminescence was measured according to the manufacturer’s instructions using a plate reader ...
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bioRxiv - Microbiology 2020Quote: ... Cell viability was measured two days post treatment with CellTiter-Glo Luminescent Cell Viability Assay kit (Promega, G7570) following standard protocol ...
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bioRxiv - Microbiology 2021Quote: Forty-nine confirmed Bp isolates were subjected to DNA extraction using the Wizard Genomic Extraction Kit (Promega, USA). For whole genome sequencing (WGS) ...
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bioRxiv - Microbiology 2021Quote: ... RNA was isolated using the Viral Total Nucleic Acid kit for the Maxwell RSC instrument (Promega, Madison, WI) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: Genomic DNA was extracted from all Salmonella isolates using the Wizard Genomic DNA Extraction Kit (Promega, Wisconsin, USA). Two μg of genomic DNA from each organism was subjected to indexed-tagged pair-end sequencing on an Illumina Hiseq 2000 platform (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... Plasmid DNA was isolated from each clone using Wizard® Plus SV Miniprep kit (Promega, Madison, WI, USA) and a total of 72 individual clones were sequenced with pJET1.2-F and pJET1.2-R primers at Eurofins Genomics Germany GmbH.
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bioRxiv - Microbiology 2021Quote: ... LDH release from lysed cells was assessed using the colorimetric CytoTox 96 Non-radioactive Cytotoxicity Assay Kit (Promega). Briefly ...
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bioRxiv - Cell Biology 2021Quote: Glucose uptake in male and female PTECs was measured with a Glucose Uptake-Glo™ Assay Kit (Promega), following manufacturer instructions (see Supplemental Methods).
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bioRxiv - Biochemistry 2021Quote: ... the cells were lysed and luciferase activity was measured using the Firefly and Renilla Luciferase Assay Kit (Promega). Relative promoter activity was computed by normalizing the Firefly luciferase activity against that of the Renilla luciferase.
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bioRxiv - Microbiology 2021Quote: Renilla luciferase (Rluc) and Firefly luciferase (Fluc) activity was measured using the dual-luciferase reporter assay kit (Promega). Measurements were performed according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... RNA was extracted from cultured cells using the Maxwell 16 Cell LEV Total RNA Purification Kit (Promega; AS1225). Corresponding cDNA was prepared using the SuperScript III First-Strand Synthesis System (Invitrogen).
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bioRxiv - Microbiology 2022Quote: ... which was performed at Baseclear with the Wizard HMW DNA Extraction kit (Promega Benelux B.V., Leiden, The Netherlands). The genome of the fast-growing isolate Pfast was sequenced using Illumina Novaseq and ONT GridiON at Baseclear (Leiden ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNA was synthesized from 100-500 ng of total RNA using the GoScript reverse transcription reaction kits (Promega). Singleplex quantitative real-time polymerase chain reaction (qPCR ...
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bioRxiv - Microbiology 2022Quote: ... The amounts of LDH were measured spectrophotometrically using a Cyto-Tox 96 Non-radioactive Cytotoxicity Assay kit (Promega). The LDH (% ...
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bioRxiv - Genetics 2020Quote: ... Genomic DNA was mass extracted from pooled samples of mosquitoes using the Wizard Genomic DNA purification kit (Promega), and 90 ng of each used for PCR using KAPA HiFi HotStart Ready Mix PCR kit (Kapa Biosystems ...
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bioRxiv - Synthetic Biology 2019Quote: ... DAS1 and DAS2 were amplified from CBS7435 wt genomic DNA (gDNA) (Promega, Wizard® Genomic DNA Purification Kit) by PCR (NEB ...
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bioRxiv - Cancer Biology 2019Quote: The parental cell lines were authenticated using short tandem repeat (STR) analysis kit PowerPlex16HS (Promega, Madison, WI, USA) through the Johns Hopkins University Genetic Resources Core Facility.
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bioRxiv - Cell Biology 2019Quote: DNA from DLD-1 and nucleoporins-targeted cells was extracted with Wizard® Genomic DNA Purification Kit (Promega). Clones were genotyped by PCR for homozygous insertion of tags with two sets of primers listed in Supplementary Table 5.
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bioRxiv - Developmental Biology 2020Quote: ... Cells were lysed 48 hr post-transfection and assayed for luciferase activity using the Dual-Luciferase Kit (Promega) according to the manufacturer’s recommended protocol.
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bioRxiv - Bioengineering 2020Quote: ... This purified plasmid was used as a template for in vitro transcription with a Ribomax transcription kit (Promega) to generate RNA of ~1488nt ...
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bioRxiv - Cell Biology 2020Quote: DNA from DLD-1 and nucleoporins-targeted cells was extracted with Wizard® Genomic DNA Purification Kit (Promega). Clones were genotyped by PCR for homozygous insertion of tags with two sets of primers listed below.
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bioRxiv - Synthetic Biology 2020Quote: ... a 1 mL aliquot was taken for genomic isolation through the Maxwell kit (Promega, Cat# AS1490, Madison WI). Successful integration was verified through qPCR ...
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bioRxiv - Synthetic Biology 2020Quote: ... a 1 mL aliquot was taken for genomic isolation using the Maxwell kit (Promega, Cat# AS1490, Madison WI). Successful integration was verified using qPCR ...