Labshake search
Citations for Promega :
4651 - 4700 of 6494 citations for 2'3' Cyclic GAMP cGAMP ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... Colorimetric Cell Viability Kit I (WST-8 reagent, PromoKine, Heidelberg, Germany) and Caspase-Glo® 8 Assay (Promega, Madison, WI) were used for cell viability assay and caspase-8 assay ...
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bioRxiv - Microbiology 2022Quote: ... We extracted genomic DNA from whole blood using a Wizard® Genomic DNA Purification Kit (#A1120, Promega Corp., Madison, USA) and then performed IPATS-BLV ...
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bioRxiv - Developmental Biology 2022Quote: ... Luciferase assays were conducted 8 hours after stimulation using a dual luciferase kit (Dual-Luciferase® Reporter Assay System, Promega) according to manufacturer’s protocol.
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bioRxiv - Evolutionary Biology 2022Quote: ... DNA was purified from the gel fragments using the Wizard SV Gel and PCR Clean Up kit (Promega, Fitchburg, USA) according to the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2022Quote: ... sequence was amplified from human genomic DNAs and used as in vitro transcription template in the reaction using Riboprobe System-T7 Kit (P1440, Promega) in the presence of 0.4μL 5-(3-Aminoallyl)-uridine-5’-triphosphate labeled with ATTO 680(Aminoallyl-UTP-ATTO-680 ...
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bioRxiv - Developmental Biology 2022Quote: ... the T7-VEE constructs were digested with Mlul and then in vitro transcribed using RiboMAX Large Scale RNA Production System Kit (Promega). The transcripts were 2’-O-methylated ...
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Zbtb14 regulates monocyte and macrophage development through inhibiting pu.1 expression in zebrafishbioRxiv - Developmental Biology 2022Quote: ... cells were harvested 48 hours after transfection and analyzed using the Dual Luciferase Reporter Assay Kit (Promega, Maddison, WI, USA), according to the manufacturer’s protocols ...
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bioRxiv - Physiology 2022Quote: ... cDNA was synthesized by using qScript cDNA Synthesis Kit (QuantaBio, Beverly, MA) and PCR was performed with GoTaq qPCR Master Mix (Promega) with HPRT as the control gene ...
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bioRxiv - Microbiology 2022Quote: ... were measured in 10 μL of each supernatant mixed with 10 μL 2X Renilla lysis buffer (RLB) by Renilla Luciferase Assay Kit (Promega) in white 96 well plates on a Berthold plate reader ...
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bioRxiv - Microbiology 2022Quote: ... Approximately 4 µg of the extracted material was then used as template to in vitro synthesized viral genomic RNA transcripts using the Ribomax T7 RNA transcription Kit (Promega) and Ribo m7G Cap Analogue (Promega ...
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bioRxiv - Molecular Biology 2022Quote: ... The firefly luciferase activity was detected in homogenates of organs by luminescence measurements using Synergy H4 hybrid reader (Bio-Tek) and Bright-Glo Luciferase Kit (Promega).
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bioRxiv - Synthetic Biology 2022Quote: ... and plasmids were extracted from individual cultures outgrown in LB/ampicillin (50 μg/mL) using the PURE Yield Plasmid Miniprep kit (Promega). Individual clones were screened and confirmed by Sanger sequencing at Macrogen-Europe B.V ...
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bioRxiv - Plant Biology 2022Quote: ... The DAP-seq gDNA library was prepared using KAPA HiFi HotStart ReadyMix with unique index primers. ZmEREB57 was fused to HaloTag using the kit from pFN19K HaloTag T7 SP6 Flexi Vecto (cat. no. G184A) (Promega). ZmEREB57 fused to HaloTag was expressed using the TnT SP6 High-Yield Wheat Germ Protein Expression System (L3260 ...
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bioRxiv - Neuroscience 2022Quote: ... cDNA was then synthesized using 2 μg of mRNA and a Transcriptase first-strand cDNA synthesis kit (Promega, Madison, WI). Real-Time qPCR to detect the indicated targets was performed on a LightCycler® 480 System (Roche Life Science ...
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bioRxiv - Genomics 2022Quote: ... DNA used for microarray was isolated from frozen cell pellets (3×106-7×106 cells) using the Maxwell RSC Cultured Cells DNA Kit on a Maxwell RSC 48 instrument (Promega). DNA was genotyped at the Children’s Hospital of Philadelphia’s Center for Applied Genomics using the Infinium Omni2.5-8 v1.3 BeadChip (Illumina ...
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bioRxiv - Neuroscience 2022Quote: Glucose uptake by cultured primary astrocytes was studied using a bioluminescent method with the Glucose Uptake Assay Kit (Promega, J1341) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... Hybridization probes were generated by PCR amplifi cation using gene specifi c primers (Table S1) and radiolabeled with the Prime A Gene labeling kit (Promega) following the recommendations of the manufacturer.
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bioRxiv - Microbiology 2022Quote: Cell viability was measured using a commercially available kit [CellTiter 96 Aqueous One Solution Cell Proliferation Assay (Promega, Madison, USA)] ...
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bioRxiv - Cell Biology 2022Quote: Three major proteasome activities (chymotrypsin-like trypsin-like, caspase-like) were measured using the Proteasome-Glo Cell-Based Assay Kit (G1180, Promega) as described by the manufacturer ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Cells were passaged every 3–4 days and regularly checked for mycoplasma infections using a GoTaq G2 Hot Start Taq Polymerase kit from Promega.
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bioRxiv - Microbiology 2022Quote: In vitro generated RNA transcripts above were used templates for in vitro translation assays using a Rabbit Reticulocyte Lysate kit (Promega) at the indicated concentrations ...
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bioRxiv - Zoology 2022Quote: ... The sperm suspension was then divided into two samples: one for determining ATP using a CellTiter-Glo Luminescent Cell Viability Assay kit (Promega) and the other for assessing DNA content (to calibrate the ATP content by the number of sperm cells ...
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bioRxiv - Molecular Biology 2022Quote: For Lytic endpoint measurements cells were seeded as mentioned above and NanoLuc abundance was determined via the Nano-Glo HiBit lytic detection kit (Promega) following manufacturers recommendations ...
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bioRxiv - Plant Biology 2024Quote: ... RNA extraction was performed using an RSC 48 RNA extraction robot (Maxwell) and the Maxwell RSC Plant RNA kit (Promega). Although the RNA yield for some of the 64 samples was low ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted using E.Z.N.A.® Plant RNA Kit (R6827-01, OMEGA) following the manufacturer’s instructions and treated with RNase-Free DNase (M6101, Promega) to remove genomic DNA contamination ...
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bioRxiv - Systems Biology 2024Quote: ... The assay was repeated three times and was conducted using the Caspase Glo® 3/7 assay kit (Promega, USA) based on the manufacturer’s recommendations.
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bioRxiv - Plant Biology 2024Quote: ... Total RNA was isolated from 30 mg of ground plant tissue using the SV Total RNA Isolation System Kit (Promega) and its concentration and integrity were assessed using the RNA 6000 Nano Kit on a Bioanalyzer (Agilent Technologies) ...
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bioRxiv - Microbiology 2024Quote: Lytic cell death was indirectly studied by measuring lactate dehydrogenase (LDH) release in cell culture medium using the CytoTox 96 nonradioactive cytotoxicity assay kit (G1780, Promega) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: Cell growth endpoint analyses were performed using CellTiter96 Aqueous Non-Radioactive Cell Proliferation MTS Assay kit (Promega, Madison, WI, USA) per manufacturer specifications ...
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bioRxiv - Microbiology 2023Quote: ... Total genomic DNA was extracted from the mid-log culture using the Wizard® Genomic DNA Purification Kit from Promega following the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2024Quote: ... The -1 PRF efficiency was assayed in cultured HEK293 as described previously (Kelly et al. 2020) using dual-luciferase reporter assay system kit (Promega). 24 hr post transfection ...
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bioRxiv - Molecular Biology 2024Quote: ... Tail DNA from offspring was genotyped for the presence of the BAC transgene by analyzing 100 ng of tail DNA with the GoTaq PCR reaction kit (Promega) according to the manufacturer’s instruction by adding forward primer 5′-GAGGCTCGGGTGCGGCAGCTC −3′ and reverse primer 5′-GTGCAGTGTGAGCACCTGCTGTC-3′ for PCR with an initial denaturation for 3 min at 95 °C ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Genomic DNA was isolated and purified using the Wizard Genomic DNA Purification Kit protocol for Gram negative bacteria at one-third volume (Promega), then quantified using SYBR Green I Nucleic Acid Stain (Invitrogen ...
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bioRxiv - Immunology 2023Quote: In situ nick-end labeling of nuclear DNA fragmentation was performed with a TUNEL apoptosis detection kit (DeadEnd Fluorometric TUNEL system, Promega) according to the supplier’s instructions.
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bioRxiv - Microbiology 2024Quote: Aliquots of the final populations were thawed and the gDNA was extracted using the Wizard Genomic DNA Purification Kit (Promega). Briefly ...
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bioRxiv - Microbiology 2024Quote: ... In vitro mRNA synthesis was then carried out at 37 °C for 4 h in T7 RiboMAX Large Scale RNA Production System kit (Promega) according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2024Quote: ... Lactate in flow-through medium was quantified using the L-lactate assay kit (Biomedical Research Service & Clinical Application, #A-108) and GloMax microplate reader (Promega) as suggested by the manufacturer ...
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bioRxiv - Cell Biology 2024Quote: 35S-methionine-labelled prey proteins (Grip91, Sas4) were produced in vitro using the TnT T7 Quick Coupled IVTT kit (L1170, Promega). The detailed protocol of the GST-IVTT binding assay is described previously 52.
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bioRxiv - Neuroscience 2023Quote: Approximately 5x104 HEK293 cells were seeded on poly-L-lysine coated glass coverslips and transfected with expression constructs for SORL1-WT or SORL1-R953C using Fugene 6 Transfection Reagent kit (Promega). 24h post-transfection ...
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bioRxiv - Biochemistry 2023Quote: ... Authentication of this cell line was performed by the Johns Hopkins Genetics Resources Core Facility by short tandem repeat analysis using the PowerPlex 16 HS kit (Promega) within a year of study ...
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bioRxiv - Systems Biology 2023Quote: The concentration of glutamine and glutamate in the media were measured using the luminescence-based approach Glutamine/Glutamate-Glo TM Assay kit (Promega). The method is based on two steps ...
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bioRxiv - Microbiology 2024Quote: ... The PCR product was extracted from 2% agarose gel and purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, USA) according to the manufacturer’s instructions and quantified using a Quantus Fluorometer (Promega, USA). Normalization was performed the counts of individual OTUs in a sample by dividing the total counts of all OTUs within that sample followed by a multiplication by resulting in relative abundance expressed.
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bioRxiv - Microbiology 2023Quote: ... The inoculum was added to HEK293T cells and incubated at 37 ° C with 5% CO2 and the luciferase signal was with analyzed using luciferase assay kit (Promega).
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bioRxiv - Cell Biology 2023Quote: ... Supernatant was transferred into a fresh tube and used to measure luciferase activity with Dual-Luciferase Reporter Assay kit (Promega) on a Lumat LB9507 luminometer (Berthold Technologies) ...
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bioRxiv - Genomics 2024Quote: ... Transfected cells were cultured under standard conditions for 72 hours before being washed with PBS and lysed and processed using materials provided with the Dual-Luciferase Reporter Assay System Kit (Promega).
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bioRxiv - Developmental Biology 2024Quote: ... Digoxigenin-labelled or Fluorescein-labelled mRNA probes were either prepared as PCR probes with primers listed in Table S4 using the Kit GoTaq green master mix (Promega) or as described (Orgeur et al. ...
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bioRxiv - Developmental Biology 2024Quote: ... For RT-qPCR four micrograms of the extracted RNA from each sample were reverse transcribed with gene specific primers using Goscript reverse transcription kit (Promega). Quantitative PCR was performed using Power SYBR Green (Life Technologies) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 1 uL of the genomic DNA extracted using a Wizard Genomic DNA Purification Kit according to the manufacturer’s instructions (A1120, Promega USA), 1 uL Q5 high-fidelity DNA polymerase ...
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bioRxiv - Microbiology 2024Quote: ... difficile strains carrying sLucopt reporter constructs was mixed with 20 µL of reconstituted Nanoglo substrate (50 fold diluted Nanoglo substrate in kit buffer, Promega) in a white ...
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bioRxiv - Systems Biology 2023Quote: ... Their identity was confirmed through cell line authentication carried out by BMR Genomics (Italy) by amplification of 23 STR loci (PowerPlex Fusion System kit, Promega). Cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM ...