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Citations for Promega :
4451 - 4500 of 6494 citations for 2'3' Cyclic GAMP cGAMP ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: HEK293T cells were collected and pelleted 72 hr post-transfection for genomic DNA extraction using the Wizard Genomic DNA Purification Kit (Promega). The target locus was PCR-amplified using AccuPrime Pfx high-fidelity DNA polymerase (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: ... The resulting libraries were checked for their quality using the High-sensitivity DNA chip using the Agilent 2100 Bioanalyzer (Waldbroon, Germany) and quantified using the QuantiFluor One dsDNA kit (Promega). Paired-end (2x300bp ...
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bioRxiv - Cell Biology 2019Quote: ... to oxidized NAD (NAD+) ratio was determined in hMPCs via a commercially available luminescence-based kit (NAD/NADH GloTM Assay, Promega) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: Glucose uptake was measured based on the detection of 2-deoxyglucose-6-phosphate uptake by a commercially available luminescence-based kit (Glucose Uptake-GloTM Assay, Promega) on a SpectraMax M3 (Molecular Devices) ...
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bioRxiv - Cell Biology 2020Quote: ... and individual colonies were inoculated in Luria-Bertani (LB) media for DNA extraction using DNA miniprep or midiprep kits (Promega). All mutations were verified by Sanger DNA sequencing (Stony Brook University Genomic Facility) ...
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bioRxiv - Cell Biology 2020Quote: ... proteins were produced from pcDNA3-based plasmids at 30 °C for 90 min using the in vitro transcription & translation (IVTT) kit (Promega), following manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... Total cell and mitochondrial free cholesterol and cholesterol ester contents were measured using the Cholesterol/Cholesterol Ester-Glo Assay Kit (J3190, Promega) according to the manufacturer’s instructions.
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bioRxiv - Genetics 2021Quote: Pooled DNA extractions (minimum 90 adult mosquitoes or 3500 L1 larvae) were performed using the Wizard Genomic DNA Purification kit (Promega). A 349 bp locus containing the predicted on-target cleavage site was amplified with primers containing Illumina Nextera Transposase adapters (underlined) ...
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bioRxiv - Microbiology 2019Quote: ... The viability of N2a cells was evaluated by Cell Titer 96 AQueous One Solution cell proliferation assay kits (Promega, G3582) according to the manufacturer’s instruction.
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bioRxiv - Molecular Biology 2021Quote: After 24 hours a luciferase assay was performed according to the instructions of the Dual-Luciferase Reporter Assay System kit (Promega) or the cells were resuspended in TRI Reagent (Sigma-Aldrich).
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bioRxiv - Developmental Biology 2021Quote: ... media was collected at a single timepoint (12 hours) and the Promega Glutamate/Glutamine-Glo kit (Promega cat. no. J8021) was used ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and from the interior tissue (at least 2 mm away from the outermost edge of the specimen) using the Wizard Genomic DNA Purification Kit (Promega), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Overnight cultures were used for genomic DNA (gDNA) preparation using Promega Wizard Genomic DNA Purification Kit (Promega, Madison, WI, USA) or Epicenter MasterPure Complete DNA and RNA Purification Kit (Lucigen ...
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bioRxiv - Genetics 2022Quote: ... instrument was configured with the Standard Elution Volume (SEV) hardware and using the Maxwell 16 Cell DNA purification kit (Promega). For total RNA isolation ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were then pelleted at 300g for 7 minutes and resuspended in 200μL of Homogenization Buffer from the Maxwell RSC simplyRNA Tissue Kit (Promega #AS1340). Cells were then lysed by adding 200μL of lysis buffer and transferred into the Maxwell RSC Cartridge ...
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bioRxiv - Molecular Biology 2021Quote: Agonist (α-MSH, afamelanotide, bremelanotide or THIQ) stimulated cAMP accumulation was measured by a GloSensor™ cAMP assay kit (Promega). Briefly ...
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bioRxiv - Molecular Biology 2021Quote: ... The mammalian expression vectors for EphA1 WT and EphA1 P460L were transfected into HEK cells using Fugene6 transfection kit (Promega). Expression of EphA1 WT or EphA1 P460L was confirmed by immunoblotting with anti-Flag or anti-Myc antibody.
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bioRxiv - Molecular Biology 2020Quote: Preserved tissue (10-15 mg) was used for nucleic acid purification by employing the Wizard genomic DNA kit (Promega, USA) and following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... then 1 µg RNA was used to synthesize the oligo(dT) primed first-strand cDNA using the M-MLV reverse transcriptase cDNA synthesis kit (Promega). Quantitative RT-PCR was performed using a CFX96 Real-Time PCR detection system (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... and the relative rDNA promoter activity was measured in cell lysates using a dual luciferase assay kit (https://worldwide.promega.com/products/luciferase-assays/reporter-assays/dual_luciferase-reporter-assay-system/?catNum=E1910, Promega, Madison, WI, USA).
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bioRxiv - Molecular Biology 2021Quote: The ATP quantification assay was performed as per the protocol for the CellTiter-Glo 3D cell viability assay kit (Promega), using 1x 104 cells per sample ...
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bioRxiv - Molecular Biology 2021Quote: ... the two blots were hybridized O/N at 65 °C to each their radioactively labeled 3’ CF probe (AGO1 3’ CF and CSD2 3’ CF probes labelled with Prime-a-gene labeling kit from Promega). The membranes were washed 3 times in 2xSSC (0.3 M NaCl ...
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bioRxiv - Microbiology 2020Quote: ... cell viability was assessed by measruring ATP levels in cells lysates with a commercially available kit (CellTiter-Glo®, Promega).
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bioRxiv - Microbiology 2020Quote: ... Transduction rates were assessed by measuring luciferase activity in cell lysates at 48 hours post transduction with a commercially available kit (Promega). Values for untreated controls were set to 100% infection.
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bioRxiv - Microbiology 2020Quote: ... Infection rates were assessed by measuring luciferase activity in cell lysates at 16 h post transduction with a commercially available kit (Promega). Values for untreated controls were set to 100% infection.
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bioRxiv - Genomics 2020Quote: ... of blood sample was collected from each individual and genomic DNA was extracted by using Maxwell RSC whole blood DNA extraction kit (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... Genomic DNA was extracted from selected clones as well as in the parental clone using the Wizard Genomic DNA Purification Kit (Promega). NGS was performed using Nextera XT DNA Library Preparation kit and the NextSeq 500 sequencing systems (Illumina ...
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bioRxiv - Genomics 2020Quote: ... After 48 h Luciferase assay was performed according to manufacturers’ instruction using the Dual-Luciferase Reporter Assay System Kit (Promega).
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bioRxiv - Genomics 2020Quote: The total RNA was extracted from Inner Mongolia Alashan cashmere goats and Dazu black goats using RNA extraction Kit (Promega), and RNA quality and concentration were measured on an Agilent 2100 Bioanalyzer (Germany) ...
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bioRxiv - Microbiology 2019Quote: ... 2 mL of cultures grown to early stationary phase and genomic DNA extracted with a Wizard Genomic DNA Purification Kit (Promega). Outside-in colony PCR of a subset of hpn genes was performed with PrimeSTAR DNA polymerase (TaKaRa ...
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bioRxiv - Neuroscience 2019Quote: ... The cells were subsequently assayed for red Firefly activity using the ONE-GloTM Luciferase Assay System kit (Promega, Madison, WI) on a Spectramax M5 (Molecular Devices ...
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bioRxiv - Neuroscience 2019Quote: ATP levels were assessed with luciferin-luciferase based assay (Lundin & Thore, 1975; McElroy & DeLuca, 1983) using a commercial ATP assay system with bioluminescence detection kit (Enliten, Promega). ATP was measured according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... All DNA extractions were done with the Wizard® SV Gel and PCR Clean Up system kit (Promega, Madison, USA).
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bioRxiv - Evolutionary Biology 2019Quote: DNA was extracted from stickleback fin clips using the Wizard® SV Genomic Purification kit (Promega® Corp., Madison, USA) and quantified using Picogreen® ds DNA assay (Thermo Fisher Scientific ...
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Mutant ACTB mRNA 3′UTR Promotes Hepatocellular Carcinoma Development by Regulating miR-1 and miR-29abioRxiv - Cancer Biology 2019Quote: ... Cells were harvested after transfection 48h and the relative luciferase activity was measured by dual-luciferase reporter assay kit (Promega).
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bioRxiv - Microbiology 2019Quote: ... Light units were measured in a Berthold LB 960 Centro microplate luminometer (Berthold Technologies) using a Renilla Luciferase Assay System kit (Promega) and MikroWin 2010 software.
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bioRxiv - Molecular Biology 2019Quote: ... Post this total RNA was extracted from the treated and control insects using SV Total RNA Isolation kit (Promega, USA). The RNA was quantified and qualified by a NanoDrop ND-2000 (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2019Quote: ... The luciferase activities of firefly and renilla were measured after 24 hours of transfection by using Dual-Glo luciferase assay kit (Promega) according to the manufacturer’s instructions.
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bioRxiv - Genetics 2019Quote: ... Beads were washed four times with IP buffer and bound nucleic acids were isolated using the Wizard SV gel and PCR clean-up kit (Promega). Collected DNA fragments were quantified using RT-PCR as described above with the following primers ...
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bioRxiv - Cancer Biology 2019Quote: ... The number of viable cells was measured after 72 hrs incubation using a CellTiter Glo Luminescent Cell Viability Assay kit (Promega) with a SpectraMax plate reader (Molecular Devices ...
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bioRxiv - Cancer Biology 2019Quote: ... Organoid viability was assessed at 7 days post-transfection using the CellTiter-Glo® Luminescent Cell Viability Assay kit (Promega), as per the manufacturer’s instructions.
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bioRxiv - Genomics 2019Quote: Genomic DNA was isolated from the tip of tails of 3-week-old mice using Wizard Genomic DNA purification Kit (Promega), amplified by PCR ...
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bioRxiv - Microbiology 2019Quote: The pBESTluc plasmid was used as a backbone for all reporter assay constructs and was included in the Luciferase Assay System kit (Promega). pBEST + metK was created by inserting nucleotides corresponding to the first 10 codons of the C ...
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bioRxiv - Microbiology 2019Quote: ... Lifted cells were resuspended with an additional 50 µl of DMEM before adding to 50 µl Nuclei Lysis Buffer (Wizard Genomic DNA Purification kit; Promega) and frozen at −20°C overnight ...
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Global genetic patterns reveal host tropism versus cross-taxon transmission of bat BetacoronavirusesbioRxiv - Evolutionary Biology 2020Quote: Genomic RNA was extracted from small intestine and large intestine samples using the SV Total RNA Isolation kit (Promega, USA) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and from the interior of the sponge tissue (<2mm from the exterior pinacoderm) and the seawater filter papers using the Wizard Genomic DNA Purification Kit (Promega).
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bioRxiv - Plant Biology 2020Quote: ... only root parts (around 100 mg) were collected and total RNA was extracted using a Trizol-adapted ReliaPrep RNA Tissue Miniprep Kit (Promega). For verifying the transcript level in single T-DNA lines ...
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bioRxiv - Genetics 2019Quote: In vitro transcription and translation were realized from 500ng of cDNA pGEM-T construct using the TnT T7 Quick Coupled Transcription/Translation kit (Promega) and Transcend Biotin-Lysine-tRNA following the manufacturer protocol ...
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bioRxiv - Genetics 2021Quote: ... The genomic DNA (gDNA) of individual transient G0 mosquitoes was extracted using the Wizard® Genomic DNA Purification Kit (Promega). In the T7 Endonuclease I (T7EI ...
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bioRxiv - Microbiology 2021Quote: ... Approximately 4 μg of the extracted material was then used as template to in vitro synthesized viral genomic RNA transcripts using the Ribomax T7 RNA transcription Kit (Promega) and Ribo m7G Cap Analogue (Promega ...