Labshake search
Citations for Promega :
401 - 450 of 578 citations for Tryptic Soy Agar TSA CRITERION Dehydrated Culture Media since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... cells were washed once in PBS and lysed in 35 μl 1x Luciferase Cell culture lysis buffer (E1531, Promega) for 15min at room temperature and centrifuged for 10min at 4°C ...
-
bioRxiv - Immunology 2023Quote: ... into the culture medium was quantitated using Cyto Tox 96® Non-Radioactive Cytotoxicity Assay (Promega, Madison, WI, USA) as described previously (20) ...
-
bioRxiv - Molecular Biology 2023Quote: ... After 3 days the cells were washed with PBS and lysed with Cell Culture Lysis Buffer (Promega cat. #E1531) prior to firefly and Renilla luciferase activity measurements which were performed in triplicate using the Dual-GLO kit (Promega cat ...
-
bioRxiv - Molecular Biology 2023Quote: ... fresh bacmid DNA was transfected into Sf9 cells at 0.5×106 cells/mL in 6-well cell culture plates using FuGene HD (Promega) according to the manufacturer’s protocol (final concentration 10 μg/mL) ...
-
Identification of echinacoside as a tobramycin potentiator against Pseudomonas aeruginosa aggregatesbioRxiv - Microbiology 2024Quote: Genomic DNA from different isolates was obtained from pelleted overnight cultures using the Wizard Genomic DNA purification kit (Promega) following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2024Quote: ... Plasmid DNA was isolated from overnight cultures using Wizard® Plus SV Minipreps DNA Purification Systems (Promega, Wisconsin, USA) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2023Quote: ... cells were washed once in PBS and lysed in 35 μL 1× Luciferase Cell culture lysis buffer (E1531, Promega) for 15 min at room temperature and centrifuged for 10 min at 4°C ...
-
bioRxiv - Immunology 2023Quote: ... 100 μl of culture medium were discarded and addition with 100 μl of Bright-Glo luciferase reagent (Promega, E6120) in each well ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... The pellet was resolved in 100 µL Cell Culture Lysis Reagent (Luciferase Assay System, E1500, Promega, Madison, WI, USA) on ice and cleared by centrifugation (8000 ×g ...
-
bioRxiv - Bioengineering 2023Quote: ... 250 rpm.10 µl of each culture was collected for the luciferase assay (Promega, Nano-Glo Luciferase Assay System). Cells were diluted in 90 µl media ...
-
bioRxiv - Developmental Biology 2023Quote: ... HEK293 culture medium was replaced by imaging medium containing either Janelia Fluor 646 (JF646) ligand (10 nM; Promega, #GA1120) or OregonGreen ligand (50 nM ...
-
bioRxiv - Immunology 2022Quote: ... CHO-K1 or HEK-293F cells (European Collection of Authenticated Cell Cultures) were transfected with plasmids using FuGene (Promega), and Fc-secreting cells cloned ...
-
bioRxiv - Microbiology 2022Quote: ... RNA was extracted from leaves or bacterial liquid cultures via phenol/chloroform extraction and treated with DNase 1 (Promega) to remove gDNA contamination ...
-
bioRxiv - Cancer Biology 2023Quote: The 3-dimensional (3D) culture studies were performed using MCF-7 cells and low melting point agarose (Promega, Australia). Agarose was suspended in PBS at a concentration of 0.75% (w/v) ...
-
bioRxiv - Cell Biology 2023Quote: DNA was prepared from bacterial cultures grown at 37°C using a Midiprep kit (PureYield Plasmid Midiprep System, Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... Total genomic DNA was isolated from overnight cultures of Ag1 and Ag2 using the Genome Wizard kit (Promega, WI), following the manufacturer’s protocol ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... The plasmid DNA was purified from 4-mL overnight cultures by use of the Wizard Plus MiniPrep kit (Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2024Quote: ... 35 uL of infected (and uninfected) cell culture supernatants were collected from each well and LDH quantified as per manufacturer’s instructions (Promega), with percent cytotoxicity calculated as a proportion of lysed control cells and blank media ...
-
bioRxiv - Microbiology 2024Quote: ATP presence was measured in cultures using the BacTiter-Glo assay according to manufacturer’s instructions with an extended incubation time (Promega). Briefly ...
-
bioRxiv - Synthetic Biology 2024Quote: ... the final A600 of the culture was measured and 20 μl of culture was diluted with 80μl of fresh LB and added to 100 μl of NanoGlo luciferase assay (Promega) consisting of NanoGlo lysis buffer supplemented with the provided furimazine substrate at a v/v ratio of 50:1 ...
-
bioRxiv - Immunology 2024Quote: The concentration of NO in the cell culture medium was measured using a Griess Assay Kit (Promega, Mannheim, Germany) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2024Quote: ... Fresh bacmid DNA was transfected into Sf9 cells at 0.5×106 cells/mL in 6-well cell culture plates using FuGene HD (Promega) according to the manufacturer’s protocol (final concentration 10 µg/mL) ...
-
An mRNA processing pathway suppresses metastasis by governing translational control from the nucleusbioRxiv - Cancer Biology 2021Quote: ... after which the media was changed and the cell viability was measured 72 hours later using CellTiter-Glo Luminescent Cell Viability Assay (Promega) in 6 biological replicates ...
-
bioRxiv - Cell Biology 2020Quote: ... and individual colonies were inoculated in Luria-Bertani (LB) media for DNA extraction using DNA miniprep or midiprep kits (Promega). All mutations were verified by Sanger DNA sequencing (Stony Brook University Genomic Facility) ...
-
bioRxiv - Developmental Biology 2021Quote: ... media was collected at a single timepoint (12 hours) and the Promega Glutamate/Glutamine-Glo kit (Promega cat. no. J8021) was used ...
-
bioRxiv - Molecular Biology 2021Quote: Total RNA from yeast strains grown in YPD media was isolated with the acidic hot phenol method 41 and treated with DNase (Promega) according to the manufacturers’ protocols ...
-
bioRxiv - Molecular Biology 2021Quote: Viral RNA was extracted from 0.2 mL of cobas PCR Media using Maxwell® 16 instrument (Promega, Madison, WI, USA) for final elution in 30μL ...
-
bioRxiv - Bioengineering 2021Quote: Cytotoxicity of monocytes cultured in hMSC-GAG media was quantified with an LDH-Glo™ Cytotoxicity Assay (Promega, Wisconsin, USA). Lactate dehydrogenase (LDH ...
-
bioRxiv - Bioengineering 2020Quote: ... The cell lysis in each well was quantified by measuring the lactate dehydrogenase (LDH) released into the media using a nonradioactive cytotoxicity assay (CytoTox 96 Non-Radioactive Cytotoxicity Assay, Promega) following the manufacturer’s manual.
-
bioRxiv - Microbiology 2021Quote: ... the media was discarded and the wells were washed with PBS before adding 50 μl of Passive lysis buffer (Promega). Cells were frozen at −20°C to facilitate complete lysis.
-
bioRxiv - Cancer Biology 2020Quote: ... and cells were lysed in a mixture of 50 µL media and 50 µL CellTiter-Glo 2.0 reagent (Promega # G9243) per well ...
-
bioRxiv - Cell Biology 2020Quote: ... Cells were resuspended at 2 x 106 cell mL-1 in complete media and HaloTag substrate conjugated to TMR (Promega) was added at a final concentration of 5 μM ...
-
bioRxiv - Cancer Biology 2021Quote: ... media in each well was replaced with 70 μl fresh medium 70 μl of Cell Titer-Glo reagent (Promega #G7570) was added ...
-
bioRxiv - Immunology 2021Quote: ... 100 mL of media was removed and replaced with 100 mL of Bright-Glo Luciferase Assay Reagent (Promega, Cat # E2620). Luciferase activity was measured on the EnSpire Plate Reader (PerkinElmer) ...
-
bioRxiv - Immunology 2023Quote: ... on day three the media was completely removed and 60 µL lysis buffer together with 60 µL luciferase substrate (Promega) was added per well ...
-
bioRxiv - Molecular Biology 2023Quote: ... The media mixture was removed from each well and the cells were lysed with a room tempered 1xGlo buffer (Promega) for 5 min at 25°C while shaking at 550 rpm ...
-
Phospholipase C β4 promotes RANKL-dependent osteoclastogenesis by interacting with MKK3 and p38 MAPKbioRxiv - Cell Biology 2024Quote: ... the existing media was removed and replaced with 100 µL of medium containing 20 µL of CellTiter 96 AQueous One solution (Promega). The cells were then incubated for 4 h at 37°C in a humified atmosphere with 5% CO2 ...
-
bioRxiv - Bioengineering 2023Quote: ... luminescence was read by removing 150 µL of media and adding 50 µL of substrate from the Nano-Glo Luciferase Assay System (N1110, Promega). The mixture was incubated for 3 minutes in a white-walled plate before analysis with a SpectraMax M3 plate reader (Molecular Devices).
-
bioRxiv - Systems Biology 2023Quote: The concentration of glutamine and glutamate in the media were measured using the luminescence-based approach Glutamine/Glutamate-Glo TM Assay kit (Promega). The method is based on two steps ...
-
bioRxiv - Molecular Biology 2022Quote: ... cells transduced with the indicated sgRNAs were seeded in a 96-well plate in 100 µL of media and 50 µl of Cell Titer Glo reagent (Promega) was added to each well and the luminescence read on the SpectraMax M5 plate reader (Molecular Devices).
-
bioRxiv - Cancer Biology 2022Quote: ... Rescue assays were performed in media supplemented with 10%(v/v) FBS as previously described using Cell Titer 96 MTS (Promega). Briefly ...
-
bioRxiv - Biochemistry 2023Quote: ... cells were starved by 50 μL Hank’s balanced salt solution for 30 min and then incubated in 50 μL CO2-independent media containing 2% GloSensor cAMP Reagent (Promega) for 1 hour ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... The stimulation was stopped by discarding the media and cell lysis was induced by addition of 1x passive lysis buffer (PLB, Promega) and horizontal shaking for 15 min at room temperature.
-
bioRxiv - Cell Biology 2024Quote: ... Tg-NLuc abundance in the lysate and media were measured using the nano-glo luciferase assay system according to the manufactures protocol (Promega). Four controls were included for the experiments including a non-targeting siRNA control ...
-
bioRxiv - Immunology 2024Quote: ... the media was replaced to 0.1 ml Opti-MEMTM and a fresh Nano-Glo® Live cell Reagent (Promega #N2011) was prepared in a 1:20 dilution ...
-
bioRxiv - Biophysics 2024Quote: ... The transfection was terminated 24 h later by removing the media and lysing the cells using 25 µl 1x Passive Lysis Buffer (Promega). For eIF1/eIF5 knockdown experiments ...
-
bioRxiv - Cancer Biology 2024Quote: ... 150μL of media was carefully removed from each well and 50μL of the CellTiter-Glo 3D cell viability assay (Promega, G9682) reagent was added ...
-
bioRxiv - Microbiology 2020Quote: ... Overnight cultures were used for genomic DNA (gDNA) preparation using Promega Wizard Genomic DNA Purification Kit (Promega, Madison, WI, USA) or Epicenter MasterPure Complete DNA and RNA Purification Kit (Lucigen ...
-
bioRxiv - Microbiology 2021Quote: ... NF54-HGL parasites were treated with 3× IC50 for two weeks in two independent experiments and cultures were monitored by luminescence readout on the BioTek Synergy 2 Plate reader using ONE-Glo reagent (Promega). Whole-genome sequencing was performed to test for single nucleotide polymorphisms (SNPs ...
-
bioRxiv - Microbiology 2021Quote: ... Cell death was determined according to the activity of lactate dehydrogenase (LDH) in the culture supernatants using a CytoTox1 Kit according to the manufacturer’s instructions (Promega, USA).