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Citations for Promega :
451 - 500 of 578 citations for Tryptic Soy Agar TSA CRITERION Dehydrated Culture Media since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... the cells were washed with PBS and lysed with 50 μL of the cell culture lysis reagent (Promega, Madison, WI). For the SARS-CoV-2 titers ...
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bioRxiv - Microbiology 2021Quote: ... Genomic DNA was also extracted from inactivated grown cultures using a DNA extraction kit according to the manufacturer’s instructions (Promega, USA).
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bioRxiv - Microbiology 2021Quote: Epithelial cytotoxicity was determined by quantification of lactate dehydrogenase activity in A549 culture supernatants at 24 h post fungal challenge using the Cytox 96 Non-Radioactive Cytotoxicity Assay kit (Promega) according to the manufacturer’s protocol and using recombinant porcine LDH enzyme (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... cells13 for 48 hours after which cells were washed with PBS and lysed with Luciferase Cell Culture Lysis 5× reagent (Promega). Nanoluc Luciferase activity in lysates was measured using the Nano-Glo Luciferase Assay System (Promega ...
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bioRxiv - Cell Biology 2020Quote: ... DRG cultures were incubated with an antibody directed against the neuronal-specific marker βIII tubulin (Promega, Madison, WI, United States) diluted 1:2000 in blocking solution ...
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bioRxiv - Molecular Biology 2021Quote: ... the culture supernatant was removed and cells were lysed by incubation for 30 min at room temperature with Cell Culture Lysis Reagent (Promega). Next ...
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bioRxiv - Microbiology 2020Quote: ... the target cells were resuspended at 3×105/mL in prewarmed culture medium that contains EnduRen live cell substrate (Promega) at a final concentration of 17 ng/ml and incubated for 30 min ...
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bioRxiv - Molecular Biology 2021Quote: ... Luciferase assays were performed on 20 μl of lysate or 1x Cell Culture Lysis Reagent in 96 well plates on the GloMax-Multi Detection System (TM297, Promega). 100 μl Luciferase Assay Reagent was added to wells ...
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bioRxiv - Immunology 2022Quote: ... cells9 for 48 hours after which cells were washed with PBS and lysed with Luciferase Cell Culture Lysis 5× reagent (Promega). Nanoluc Luciferase activity in lysates was measured using the Nano-Glo Luciferase Assay System (Promega ...
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bioRxiv - Immunology 2022Quote: ... for 48 h after which cells were washed with PBS and lysed with Luciferase Cell Culture Lysis 5× reagent (Promega). Nanoluc Luciferase activity in lysates was measured using the Nano-Glo Luciferase Assay System (Promega ...
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bioRxiv - Microbiology 2020Quote: ... The 50% tissue culture infectious dose (TCID50) of SARS-CoV-2 pseudovirus was determined using the Steady-Glo luciferase assay system (Promega).
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bioRxiv - Immunology 2022Quote: ... for 48 hours after which cells were washed with PBS and lysed with Luciferase Cell Culture Lysis 5× reagent (Promega). Nanoluc Luciferase activity in lysates was measured using the Nano-Glo Luciferase Assay System (Promega ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... the cells were washed with PBS and lysed with 50 μL of the cell culture lysis reagent (Promega, Madison, WI). For the SARS-CoV-2 titers ...
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bioRxiv - Molecular Biology 2021Quote: ... the culture supernatant was removed and cells were lysed by incubation for 30 min at room temperature with Cell Culture Lysis Reagent (Promega). Next ...
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bioRxiv - Microbiology 2021Quote: ... culture supernatant was removed from each well and replaced with 0.3 ml of ONE-Glo luciferase reagent (Promega, Madison, WI). The plates were shaken at 400 rpm for 10 min at room temperature ...
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bioRxiv - Pathology 2021Quote: ... Cell viability was monitored by measuring lactate dehydrogenase (LDH) leakage into the culture medium by using the CytoTox96 Non-Radio Cytotoxicity Assay kit (Promega). For adherent cells (HFFs ...
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bioRxiv - Microbiology 2021Quote: ... resuspended in 50 μL (fLuc assays) or 100 μL (NanoLuc assays) of 1× Cell Culture Lysis Reagent (Luciferase Assay System; Promega) and incubated for 10 min at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were washed twice with PBS 48 hr post infection and lysed with Luciferase Cell Culture Lysis 5x reagent (Promega). Relative luminescence units were normalized to those derived from cells infected with SARS-CoV-2 pseudotyped virus in the absence of Ace2-Fc/Ace2 monomer ...
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bioRxiv - Biochemistry 2020Quote: ... the culture medium was aspirated and 20 μL of cell lysate was added from the Luciferase Assay System (Promega, E1500) to each well ...
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bioRxiv - Immunology 2021Quote: ... The mixture was subsequently incubated with 293TAce2 cells for 48 h after which cells were washed with PBS and lysed with Luciferase Cell Culture Lysis 5× reagent (Promega). Nanoluc Luciferase activity in lysates was measured using the Nano-Glo Luciferase Assay System (Promega ...
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bioRxiv - Immunology 2020Quote: ... The mixture was subsequently incubated with 293TACE2 cells for 48 hours after which cells were washed twice with PBS and lysed with Luciferase Cell Culture Lysis 5x reagent (Promega). Nanoluc Luciferase activity in lysates was measured using the Nano-Glo Luciferase Assay System (Promega) ...
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bioRxiv - Immunology 2020Quote: ... At 48 h later the cells were washed with PBS and lysed with Luciferase Cell Culture Lysis 5× reagent (Promega). Nanoluc Luciferase activity in lysates was measured using the Nano-Glo Luciferase Assay System (Promega ...
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bioRxiv - Immunology 2021Quote: ... caspase-1 activity in the cell culture supernatants was measured using Caspase-Glo® 1 inflammasome assay according to the manufacturer’s instructions (Promega). Luminescence was measured using an Omega plate reader.
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bioRxiv - Developmental Biology 2023Quote: ... After 7 days of culture the MTS cell viability reagent (CellTiter 96® Aqueous One Solution Cell Proliferation Assay, Promega) was added and plates incubated for 4 hours at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... cells were plated on three 10 cm tissue culture dishes per condition and transfected 24 h before lysis using FuGENE 6 (6-12 μg total plasmid DNA; Promega). Published protocol can be found on Protocols.io (dx.doi.org/10.17504/protocols.io.kxygx3zeog8j/v1).
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bioRxiv - Cancer Biology 2023Quote: ... where 20 μl of yeast culture was mixed in each well with an equal volume of 2x Passive Lysis Buffer (Promega) to permeabilize cells by 15 minutes of shaking at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... and genomic DNA was isolated from overnight cultures (5 mL) using the Wizard Genomic DNA Purification Kit (Promega GmbH, Walldorf) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The bacterial cells were collected from 2mL of the culture and the genomic DNA was extracted using the Wizard Genomic DNA Purification Kit (Promega). Samples were then sent to Novogene Co. ...
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bioRxiv - Cancer Biology 2022Quote: ... then cell viability was evaluated in culture based on quantification of the ATP present using CellTiter Glo Luminescent cell viability assay (Promega). Raw luminescence was recorded using a CLARIOStar (BMG Labtech ...
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bioRxiv - Genetics 2024Quote: ... We prepared triplicate tissue homogenates from ten F1 progeny from each cross using the Luciferase Cell Culture Lysis 5X Reagent (Promega) to extract total proteins by following the quick-freeze homogenization method outlined by the manufacturer ...
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bioRxiv - Microbiology 2023Quote: ... Total genomic DNA was extracted from the mid-log culture using the Wizard® Genomic DNA Purification Kit from Promega following the manufacturer’s protocol.
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bioRxiv - Neuroscience 2024Quote: Total RNA was isolated from NMRI mouse cortical neuron cultures at DIV1 and DIV20 using the Reliaprep RNA Miniprep system (Promega) followed by deoxyribonuclease I (NEB ...
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bioRxiv - Microbiology 2023Quote: ... The cell culture supernatant was collected and subjected to analysis using the CytoTox 96 assay (Promega, Madison, WI, USA; G1780) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Cells were infected the next day with culture supernatants and lysed 48 hpi followed by measuring luminescence using the Steady-Glo luciferase assay system (Promega) per the manufacturer’s recommendation and a Biostack4 (BioTek ...
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bioRxiv - Microbiology 2024Quote: Lytic cell death was indirectly studied by measuring lactate dehydrogenase (LDH) release in cell culture medium using the CytoTox 96 nonradioactive cytotoxicity assay kit (G1780, Promega) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... or HT1080/Ace2 cl14 cells (for all variant neutralization assays) for 48 hours after which cells were washed with PBS and lysedwith Luciferase Cell Culture Lysis 5× reagent (Promega). Nanoluc Luciferase activity in lysateswas measured using the Nano-Glo Luciferase Assay System (Promega ...
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bioRxiv - Synthetic Biology 2022Quote: ... and plasmids were extracted from individual cultures outgrown in LB/ampicillin (50 μg/mL) using the PURE Yield Plasmid Miniprep kit (Promega). Individual clones were screened and confirmed by Sanger sequencing at Macrogen-Europe B.V ...
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bioRxiv - Microbiology 2023Quote: Viral stocks were generated in tissue culture by transfecting MCMV genome-carrying BACs into NIH3T3 cells (ATCC CRL-1658) using FuGene HD (Promega). Reconstituted viral particles were passaged five times on M2-10B4 cells (ATCC CRL-1972 ...
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bioRxiv - Bioengineering 2023Quote: ... 2000 transfected cells were replated into white 384-well tissue culture plates in the presence or absence of 1 μM HaloTag NanoBRET-618 Ligand (Promega) and incubated overnight at 37 °C ...
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bioRxiv - Microbiology 2023Quote: Genomic DNAs were purified from 600 µL of overnight bacterial culture using the Wizard HWM genomic DNA prep kit (Promega) following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Bacterial genomic DNA was extracted from static overnight cultures of WS clones using the Wizard Genomic DNA purification kit (A1125, Promega) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: Stereocaulon virgatum RNA was extracted from the fungal culture using the Maxwell® RSC Plant RNA Kit (Promega, Madison, WI). Tissue was homogenized in 30 second intervals for 1.5 minutes at 30hz ...
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bioRxiv - Immunology 2024Quote: Pyroptotic cell death was measured by assessing LDH-release in the cell culture supernatant of human and murine macrophages using a CytoTox 96 Non-radioactive Cytotoxic Assay (Promega) following the manufacturer’s recommended procedures.
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bioRxiv - Cell Biology 2024Quote: 2-3 days before the recording the cultures were transferred into a recording medium containing 5% FBS and 0.1 mM luciferin (Promega E1601) (Feeney et al. ...
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bioRxiv - Microbiology 2024Quote: ... Pseudovirus titers were determined by infecting TZM-bl-luciferase reporter cell lines and estimating 50% tissue culture infectious dose (TCID50) using the Bright-Glo luminescence reporter gene assay system (Promega)73 as directed by the manufacturer.
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bioRxiv - Microbiology 2024Quote: Release of LDH in culture medium was quantified using the CytoTox 96® Non-Radioactive Cytotoxicity Assay (Promega, #PR-G1780) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... The 50% tissue-culture infectious dose (TCID50) of SARS-CoV-2 pseudovirus was determined using the Steady-Glo Luciferase assay system (Promega).
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bioRxiv - Immunology 2024Quote: ... the presence of NLuc in the culture supernatants of cells infected with Nluc-expressing viruses was quantified using Nano-Glo luciferase substrate (Promega) following the manufacturer’s specifications and a FLUOstar Omega microplate reader (BMG Labtech).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the cDNA were transient transfected together with 0.5 µg of the reporter plasmid EBO-pcD-Leu2-CD8 (to select transfected cells) per 35 mm culture dish24 in cells at 60-80% confluence following the FuGENE6 transfection method (Promega). The ratio µg DNA:µl Fugene was 1:3 ...
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bioRxiv - Microbiology 2024Quote: ... 100 µL of the culture was mixed with 10 µL with the NanoLuc Reaction Buffer of the Nano-Glo Luciferase Assay System (Promega). Luciferase activities were measured every 2 minutes with a Tecan Spark microtiter plate ...