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Citations for Promega :
401 - 450 of 1165 citations for RT qPCR Master Mixes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... qRT-PCR was performed by using GoTaq® qPCR Master Mix (Promega Corporation, Madison, Wisconsin, EUA) accordingly to the manufacturer instructions ...
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CRISPR screens for lipid regulators reveal a role for ER-bound SNX13 in lysosomal cholesterol exportbioRxiv - Cell Biology 2021Quote: ... qRT-PCR was performed using SYBRGreen-based technology GoTaq® qPCR master-mix (Promega, Cat# A600A). Specific SNX14 primers ...
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bioRxiv - Plant Biology 2021Quote: ... The qRT-PCR was performed in a reaction containing GoTaq® qPCR Master Mix (Promega, USA), cDNA ...
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bioRxiv - Cancer Biology 2022Quote: ... Amplifications were performed in duplicates using GO taq QPCR master mix (Promega, A6002, lot no. 0000385100) and light cycler 480 II (Roche ...
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bioRxiv - Cancer Biology 2023Quote: ... Then prepared cDNA was subjected to real-time PCR analysis with Eastep qPCR Master Mix (Promega) and primer mixtures (primer sequences are shown in table S2) ...
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bioRxiv - Cell Biology 2023Quote: ... and served as the template for quantitative PCR (using GoTaq qPCR Master Mix, A6002, Promega, USA). Then ...
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bioRxiv - Microbiology 2023Quote: ... Quantitative real-time PCR was performed using the 2x GoTaq qPCR master mix (Promega, Walldorf, Germany) with 10 ng of cDNA template and 0.5 µM of each oligonucleotide in a final volume of 15 µL ...
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bioRxiv - Cell Biology 2023Quote: ... primers designed for the gene of interest (Table 1) and GoTaq(R) qPCR Master Mix (Promega). Samples were run on an AB 7300 Real Time PCR System (Applied Biosystems ...
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bioRxiv - Molecular Biology 2023Quote: ... Diluted RNA was assayed for AGO2 transcript using the GoTaq 1-Step RT-qPCR System (Promega A6020) with primers CTGGCTCCAGGGGACAAG (Forward primer ...
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bioRxiv - Plant Biology 2021Quote: ... RT-PCR was performed with the GoTaq® Green Master Mix as per manufacturer’s instructions (Promega, USA). For RIP analysis ...
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bioRxiv - Bioengineering 2021Quote: ... The results were calculated using the 2-△△CT method according to the GoTaq qPCR Master Mix (Promega) manufacturer’s specifications.
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bioRxiv - Developmental Biology 2022Quote: ... The results were calculated using the 2-ΔΔCT method according to the GoTaq qPCR Master Mix (Promega) manufacturer’s specifications.
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bioRxiv - Immunology 2020Quote: ... Bacterial groups in cecal samples were measured by real-time PCR using GoTaq qPCR Master Mix (Promega) on Applied Biosystem 7900HT Fast or StepOne Plus™ Real-Time PCR Systems ...
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bioRxiv - Molecular Biology 2019Quote: ... PCR amplification was performed in 10 μL reactions comprising of 1X GoTaq® qPCR master mix (Promega), 1 μM of gene specific forward and reverse primers (sequences are provided in Supplementary Table 3 ...
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bioRxiv - Genomics 2021Quote: ... Diluted cDNA (1:20) was used in a PCR reaction with the GoTaq qPCR Master Mix (Promega) and run on a CFX384 Touch instrument (Bio-Rad).
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bioRxiv - Bioengineering 2021Quote: ... The results were calculated using the 2-△△CT method according to the GoTaq qPCR Master Mix (Promega) manufacturer’s specifications.
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bioRxiv - Cell Biology 2021Quote: The qRT-PCR reaction was carried out with GoTaq®qPCR Master Mix (Promega, Madison, WI, USA) on a CFX96 instrument (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: ... Then the eluted DNA was subjected to real-time PCR analysis with Eastep qPCR Master Mix (Promega) and promoter-specific primer mixtures ...
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bioRxiv - Genetics 2022Quote: Quantitative PCR was performed on the Roche LightCycler 96 instrument by using GoTaq qPCR Master Mix (Promega) with 1µl cDNA template and 0.5µl of 10mM each primer in a total volume of 25µl reaction ...
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bioRxiv - Physiology 2023Quote: ... Each 10 µl PCR reaction consisted of 5 µl of GoTaq® qPCR Master Mix (Promega Corporation), 0.5 µl of 10 µM forward primer ...
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bioRxiv - Cancer Biology 2021Quote: ... cDNA was generated from 20-100 ng of RNA using the GoTaq 2-step RT-qPCR System (Promega). qPCR was performed with SYBR Green on a StepOnePlus real-time PCR system (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2021Quote: ... cDNA was generated from 20-100 ng of RNA using the GoTaq 2-step RT-qPCR System (Promega). qPCR was performed with SYBR Green on a StepOnePlus real-time PCR system (Applied Biosystems) ...
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bioRxiv - Immunology 2021Quote: ... qRT-PCR was performed in 12 μL reactions using GoTaq Probe 1-step RT-qPCR System (Promega, US) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Real-time PCR was carried out using the Promega GoTaq® Probe 1-Step RT-qPCR System (Promega) with Sarbecovirus-specific envelope gene primers from bat RNA samples as published by (Corman ...
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bioRxiv - Microbiology 2023Quote: Real-time PCR was carried out using the Promega GoTaq® Probe 1-Step RT-qPCR System (Promega) with Sarbecovirus-specific envelope gene primers from bat RNA samples as published by (Corman ...
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bioRxiv - Microbiology 2024Quote: ... Relative quantification of viral RNA was performed using the GoTaq® 1-Step RT-qPCR System kit (Promega). The mixture contained 5 μL of 2x Master Mix ...
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bioRxiv - Immunology 2024Quote: ... To promote transfection mixes were supplied by Fugene HD transfection reagent (Promega Madison WI). On the third day ...
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bioRxiv - Microbiology 2023Quote: ... RNA reverse transcription and cDNA amplification was then carried out by the GoTaq 1-Step RT-qPCR System (Promega), using established primers (31) ...
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bioRxiv - Microbiology 2019Quote: ... Quantitative real-time polymerase chain reaction (PCR) was performed for CGRP with GoTaq® qPCR Master Mix (Promega) using the DNA-binding dye ...
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bioRxiv - Genomics 2019Quote: ... We then set up quantitative PCR reactions with 5 µL GoTaq qPCR Master Mix (Promega, Madison, WI, USA), 1 µL each forward and reverse primer at 5 µM ...
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bioRxiv - Immunology 2021Quote: ... IL-12 and TNFα were analyzed by semiquantitative PCR (SYBR Green assay Go Taq qPCR Master Mix; Promega) with specific primers (Metabion ...
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bioRxiv - Immunology 2022Quote: ... MCMV-DNA was quantified by real-time PCR using BIO-RAD CFX with GoTaq qPCR Master Mix (Promega) and primers specific for MCMV glycoprotein B (gB ...
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Mitochondrial Apolipoprotein MIC26 is a metabolic rheostat regulating central cellular fuel pathwaysbioRxiv - Cell Biology 2023Quote: ... quantitative real-time PCR was performed in Rotor Gene 6000 (Corbett Research) using GoTagR qPCR Master Mix (Promega) according to manufacturer’s instructions with the following primers:
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bioRxiv - Genetics 2024Quote: ... 1 μL cDNA was used in 10-μL reactions containing Brilliant III SYBR Green qPCR Master Mix (Promega). Biological replicates were analyzed as duplicates ...
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bioRxiv - Genetics 2024Quote: 2000ng DNA (fixed or non-fixed) was mixed with 10μL Brilliant III SYBR Green qPCR Master Mix (Promega) and the DNA melting behaviour was analysed in the CFX96 Touch Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Plant Biology 2020Quote: ... Quantitative real time RT-PCR (qPCR) was performed using gene-specific primers (Supplemental Table 1) and the fluorescent dye SYBR Green (Promega). All quantifications were normalized to the TIP41 (AT4G34270 ...
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bioRxiv - Cancer Biology 2023Quote: ... The isolated RNAs (20 ng) were reverse-transcribed and quantitative PCR (qPCR) were perform using GoTaq Probe 1-Step RT-PCR system (Promega) in a QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Immunology 2023Quote: ... cDNA was generated from 20-100 ng of RNA using the GoTaq 2-step RT-qPCR System (Promega, cat# A6110). qPCR was performed with SYBR Green on a StepOnePlus real-time PCR system (Applied Biosystems) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... QRT-PCR reactions were performed by using the GoTaq®1-Step RT-qPCR System (Promega Scientific, Madison, WI, USA) with Bio-Rad CFX Connect (Bio-Rad Laboratories ...
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bioRxiv - Molecular Biology 2021Quote: ... All the reactions were performed in a CFX-Connect real-time PCR detection system (Bio–Rad, www.bio-rad.com/) using GO Taq qPCR master mix (Promega, USA). Each experiment was repeated at least three times ...
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bioRxiv - Immunology 2022Quote: ... on memory CD8 T cells were quantified by qRT-PCR using a final 0.5 μM concentration of each primer and SYBR Green GoTaq qPCR Master Mix (Promega). Amplification was performed in a StepOne Plus Real time PCR system (Applied Biosystems) ...
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CASC3 promotes transcriptome-wide activation of nonsense-mediated decay by the exon junction complexbioRxiv - Molecular Biology 2020Quote: ... Quantitative real time PCR was performed with 16 ng of cDNA per reaction with GoTaq qPCR Master Mix (Promega) and the CFX96 Touch Real-Time PCR Detection System (Biorad) ...
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bioRxiv - Physiology 2020Quote: ... cDNA was added as 100 ng/reaction to SYBR green based GoTaq® qPCR Master Mix (Promega, Wisconsin, USA). The primer sequence for CEBPa was F ...
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bioRxiv - Cell Biology 2021Quote: The viral titers were determined by quantitative real-time PCR using GoTaq® qPCR Master Mix (Promega, Madison, WI) on a CFX96 Touch™ Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Pathology 2020Quote: ... All reactions were performed as biological triplicates and technical duplicates using a GoTaq® qPCR Master Mix Kit (Promega). The primers were designed according to the ARG gene sequence (LinJ.35.1490 ...
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bioRxiv - Cancer Biology 2020Quote: ... cDNA was diluted 1:5 in nuclease free water and 2μl of the diluted product was used for real time PCR with the following reagents: 5μl of GoTaq qPCR Master Mix (Promega), 2μl of nuclease free water and 1μl of 10mM primer mix ...
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bioRxiv - Molecular Biology 2021Quote: ... 3βHSD and FSHR in the supernatant of cultured GCs were quantified using GoTaq® qPCR Master Mix Kit (Promega) as previously described [29] ...
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bioRxiv - Genomics 2021Quote: ... All the reactions were performed in a CFX-Connect real-time PCR detection system (Bio–Rad, www.bio-rad.com/) using GO Taq qPCR master mix (Promega, USA). Each experiment was repeated at least three times ...
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bioRxiv - Molecular Biology 2023Quote: ... 1/20th of the resulting cDNA was used then analyzed by qRT-PCR using GoTaq qPCR master mix (Promega). The following primer pairs were used:
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bioRxiv - Evolutionary Biology 2023Quote: ... Denaturated RNA (5μg) was retrotranscribed and used for quantitative PCR using GoTaq qPCR Master Mix according to the manufacturer’s instructions (Promega) at a dilution of 1/10 for mycelium and 1/7 for rice leaves ...