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Citations for Promega :
401 - 450 of 4684 citations for 2' Chloro 4 5 5 dimethyl 1 3 dioxan 2 yl butyrophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... 5 ug sheared salmon sperm DNA (Promega, Madison, WI), 500 ng of RNA bait ...
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bioRxiv - Cancer Biology 2024Quote: ... 5 μL of 5X M-MLV reaction buffer (Promega), 5 μL of a mixture of 10 mM dNTPs and 200 U of M-MLV reverse-transcriptase (Promega ...
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bioRxiv - Microbiology 2022Quote: ... the virus-induced cytopathogenic effect was measured colorimetrically by the formazan-based 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) cell viability assay (CellTiter 96 AQueous One Solution Cell Proliferation Assay from Promega, Madison, WI), and the antiviral activity was expressed as the 50% effective concentration (EC50) ...
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bioRxiv - Genetics 2023Quote: ... 3,500,000 cells were seeded in 10 cm plates (2-4 per replicate) and transfected with FuGENE® 6 Transfection Reagent (Promega, E2692). In one tube ...
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bioRxiv - Cell Biology 2024Quote: ... protein samples were then incubated with chymotrypsin at a ratio of 1:80 (enzyme to protein) for 3-4 h at RT and then trypsin (Promega) at a ratio of 1:80 (enzyme to protein ...
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bioRxiv - Immunology 2020Quote: ... After a migration time of 4 h at 37°C (5 % CO2) lower compartment were analyzed for cell content by Cell-Titer-Glo® assay (Promega, Madison, WI, USA). Percentages of migrated cells were calculated as described before45 and normalized to the CXCL12-only control.
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bioRxiv - Cancer Biology 2020Quote: ... was determined at day 3 and 4 post-transfection using the Caspase-Glo 3/7 Assay (Promega, Mannheim, Germany). The emerging fluorescence was detected (485Ex/527Em ...
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bioRxiv - Cell Biology 2024Quote: ... each 5 nL of injection volume contained: 70 pg of Anillin-Halo and 5 µM Janelia Fluor® 646 HaloTag® Ligand (Promega, #GA1120), or 70 pg of Anillin-3xGFP ...
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bioRxiv - Neuroscience 2020Quote: ... all cells were incubated in the following: mouse anti-βIII tubulin (1:1000; 2 hr; Promega) and goat anti-mouse Alexa 546 (1:1000 ...
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bioRxiv - Systems Biology 2021Quote: ... Both 1° and 2° assays were performed with 2x PCR master mix (Promega Corporation, Wisconsin, USA). The final PCR product from the 2° nested PCR was checked by electrophoresis in a 1.5% agarose gel ...
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bioRxiv - Immunology 2021Quote: ... Purified mAb (2 mg/mL in PBS) was treated with 1 unit of IdeS protease (Promega) per 1 µg of mAb ...
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bioRxiv - Plant Biology 2022Quote: ... for 2 h as primary antibody and peroxidase-conjugated goat anti-rabbit antibody (1:10,000; Promega) for 1 h with 5 times 10 min washes in-between the incubations ...
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bioRxiv - Immunology 2024Quote: ... 1 mL of warm (37-40°C) 2% agarose low-melting point (Promega® Cat. V2111) was infused through the trachea into the lungs using a polythene tube with a 1.5 mm diameter (Portex Cat ...
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bioRxiv - Biochemistry 2021Quote: ... Genomic DNA was eliminated by incubating 2 μg of RNA with 2 U of RQ1 RNase-free DNase I (Promega) for 30 min at 30°C ...
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bioRxiv - Molecular Biology 2021Quote: ... FASP was carried out in two 10 kDa MWCO filters with a 50 mM iodoacetamide alkylation step and proteins were digested in 2M urea with 2% wt/wt Lys-C (Wako) for 6 h and 2% modified trypsin (Promega) for 12 h at 37°C ...
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bioRxiv - Genetics 2022Quote: ... Vectors coding for repair template DNA of roughly 2 kb were generated from Drosophila Schneider 2 cell genomic DNA by PCR using GoTaq G2 Polymerase (Promega) and primer pairs appropriate for the desired region (Supplementary Table S1) ...
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bioRxiv - Microbiology 2022Quote: 293T cells were co-transfected with pEGFP-N1 and plasmids expressing SARS-CoV-2 Spike (FL) or SARS-CoV-2 Spike-Δ19 for 24 h using FugeneHD (Promega). The cells were treated with DMSO or 10 µM 6-TG at 4 h post- transfection ...
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bioRxiv - Biochemistry 2022Quote: ... 3.5 mM MgCl2, and 2 mM ATP (for nucleic acid substrates containing RNA, 2 U/μl of RNasin Ribonuclease Inhibitor (Promega)) for 30 min at 37 °C ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were then digested overnight with 2 µg of lysyl endopeptidase (Wako) at room temperature and further digested overnight with 2 µg trypsin (Promega) at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: Proteins bound to beads were reduced with 2 mM DTT in 2 M urea buffer and sequencing grade trypsin (Promega) was added to a final concentration of 5 ng/μl ...
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Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: ... The pellet was washed with 2 ml nuclei storage buffer and resuspended in 0.2 ml PBS (calcium and magnesium-free) with 2% BSA and 0.2 U/μl RNasin® Plus RNase inhibitor (Promega). Buffer composition was described before72 ...
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bioRxiv - Neuroscience 2023Quote: ... Blots were then incubated with blotting buffer containing 1:200 LgBiT for 1-2 h with rocking at RT (N2410, Promega). Next ...
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bioRxiv - Biochemistry 2023Quote: ... pLH3/DTX3L or pLH3/DTX3LΔN) and accessory plasmids pMD2g and psPAX2 (ratio 2:1:1) using ViaFect transfection reagent (Promega E498A). After cell incubation at 37°C for ∼16 h ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 µl RNasin Plus (Promega, catalog no. N2615), 0.8 µl 100 mM ATP (ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... using 2 µg of sequencing grade trypsin (Promega) per fraction 55 ...
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bioRxiv - Biochemistry 2022Quote: ... either 2 µg pGL4.47[luc2P/SIE/Hygro] (Promega) or pGL4.52[luc2P/STAT5RE/Hygro] (Promega ...
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bioRxiv - Molecular Biology 2021Quote: ... 2) pRL Renilla luciferase control reporter (Promega, E2241) and 3 ...
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bioRxiv - Cancer Biology 2021Quote: ... pGL4.10 (Luc.2; E665A) was purchased from Promega. The pGL4.10-ITGB1 reporter and a control Renilla luciferase vector were co-transfected into breast cancer cell lines using Lipofectamine 3000 transfection kit (Invitrogen ...
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bioRxiv - Developmental Biology 2021Quote: ... plus 2 uL of random primers (Promega, C1181). This was then incubated at 70 °C for 10 mins ...
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bioRxiv - Biochemistry 2022Quote: ... 2 U/μl of RNasin Ribonuclease Inhibitor (Promega)) for 20 min on ice ...
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bioRxiv - Microbiology 2022Quote: ... 2 µM dNTPs and MMLV reverse transcriptase (Promega). Primer extension reactions to analyze initiation from the +1 and +3 sites of the le promoter were subjected to electrophoresis in 6% polyacrylamide gels containing 7 M urea in Tris-borate-EDTA buffer ...
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bioRxiv - Biochemistry 2023Quote: Cell viability assays using CellTiter-Glo 2 (Promega) were performed according to the manufacturers’ instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... we used CellTiter Glo 2 assay systems (Promega). Approximately 2.0 × 103 cells/well were seeded in a 96-well plate and incubated in RPMI1640 culture medium over 4 days at 37 °C and 5% CO2 ...
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bioRxiv - Systems Biology 2023Quote: ... 2 µl of 5x MTase-Glo Reagent (Promega) was added to wells ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 2 µL of sequencing grade modified trypsin (Promega) at a concentration of 0.1 µg/μL and 23 µL of 50mM ammonium bicarbonate/0.01% ProteaseMax were added ...
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bioRxiv - Microbiology 2023Quote: ... 2 µL of 100 bp DNA Ladder (Promega) was added to confirm amplicons size and run at 100 V for 45 mins ...
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bioRxiv - Molecular Biology 2023Quote: ... and treated with 2 μg trypsin (Promega, v511c), shaking overnight at 700 rpm ...
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bioRxiv - Molecular Biology 2023Quote: ... using the CellTiter-Blue assay (G8081/2, Promega) following the protocol of the manufacturer using an EnVision multimode plate reader (PerkinElmer) ...
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bioRxiv - Plant Biology 2024Quote: ... 2 µL ROX (CRX reference dye, Promega, C5411) were added to 1 mL SybrGreen as a passive reference dye that allows fluorescent normalization for qPCR data ...
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bioRxiv - Plant Biology 2024Quote: ... 2 µL ROX (CRX reference dye, Promega, C5411) were added to 1 mL SybrGreen as a passive reference dye that allows fluorescent normalization for qPCR data ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 μg/ml of herring sperm DNA (Promega) in dilution solution ...
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bioRxiv - Plant Biology 2024Quote: ... 2 µL ROX (CRX reference dye, Promega, C5411) were added to 1 mL SybrGreen as a passive reference dye that allows fluorescent normalization for qPCR data ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 µg of sequencing grade modified trypsin (Promega) was added ...
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bioRxiv - Molecular Biology 2024Quote: ... the dual luciferase reporter psiCHECK-2 vector (Promega) was used ...
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bioRxiv - Biochemistry 2024Quote: ... and 2 EDTA-free Protease inhibitor tablet (PROMEGA)) via probe sonication (10s ON/ 10s OFF at 60% amplitude ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was diluted 1:5 before quantitative analysis by qPCR was done using GoTaq qPCR Master Mix (Promega) following manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2020Quote: ... 8 μl 5x PNK pH 6.5 buffer [350 mM Tris-HCl pH 6.5, 50 mM MgCl2, 5 mM DTT], 1 μl PNK enzyme [NEB], 0.5 μl RNasin [Promega] ...
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bioRxiv - Plant Biology 2021Quote: ... The powder was resuspended in 30 mL of Extraction Buffer 1 (10mM Tris-HCl pH8; 0.4M sucrose; 10 mM MgCl2; 5 mM BME; 0.2 mM PMSF; RNasin PROMEGA) and was filter thought a Nylon membrane of 150 μm and centrifuged at 2000 g for 20 min at 4 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... 150 mM NaCl, 1 mM MgCl2, 2 mM EDTA, 1% NP-40, 1 mM DTT, 100 U/mL RNasin [Promega], 1X protease inhibitor) at 7 dpi ...
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bioRxiv - Molecular Biology 2020Quote: ... Apoptosis was assessed by incubating the cells with 200nM Staurosporin or medium for 4 h and the caspase 3/7 activity was assayed using the ApoOne Caspase 3/7 Assay (Promega). Senescence associated β-Galactosidase activity was analyzed with the Senescence Associated β-Galactosidase Staining Kit (Cell Signalling Technologies) ...