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Citations for Promega :
4201 - 4250 of 5345 citations for 6H Imidazo 4 5 1 de acridin 6 one 5 3 diethylamino propyl amino 8 hydroxy since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... cDNAs were synthesized from 1 μg of total RNA using AMV reverse transcriptase (Promega) and used directly for quantitative PCR (qPCR ...
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bioRxiv - Molecular Biology 2021Quote: ... and 1 μg of mass spectrometry grade trypsin/Lys-C mix (Promega CAT# V5071) in 60 μl of 50 mM ammonium bicarbonate was added to each sample ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were digested at 37 °C overnight with trypsin (Promega; 1:10, enzyme/substrate) in the presence of 10% acetonitrile ...
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bioRxiv - Microbiology 2022Quote: ... diluted 1:2 with PBS and read on a GloMax Multi+ Detection System (Promega).
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bioRxiv - Systems Biology 2022Quote: ... and RNA (1.5μg) was treated with RQ1 Dnase I (Promega; 1 U/µg RNA). cDNA was synthesized with a qPCRBIO high-quality cDNA synthesis kit ...
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bioRxiv - Microbiology 2022Quote: ... cDNAs were synthesized from 1 μg of total RNA using AMV reverse transcriptase (Promega) and used directly for quantitative PCR (qPCR ...
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bioRxiv - Microbiology 2022Quote: ... Digestion was performed with trypsin in a ratio of 1:20 m:m protein:trypsin (Promega, P/N V5072 for the pilot study ...
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bioRxiv - Microbiology 2020Quote: ... cDNAs were synthesized from 1 μg of total RNA using AMV reverse transcriptase (Promega), and used directly for quantitative PCR (qPCR ...
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bioRxiv - Molecular Biology 2020Quote: ... Transfected cells were lysed in 12.6 μl of 1 × Passive Lysis Buffer (PLB, Promega) and light emission was measured following injection of 25 μl of either Renilla or Firefly luciferase substrate ...
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bioRxiv - Neuroscience 2021Quote: ... Cells used for growth cone analyses were stained for βIII-tubulin (1:1000, Promega), Phalloidin 568 conjugate (1:500 ...
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bioRxiv - Cell Biology 2021Quote: ... 1 μg cellular RNA was treated with DNase (RQ1 Rnase-Free DNase kit Promega) and reverse-transcribed following the GoScript ™ Reverse Transcriptase Kit (Promega ...
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bioRxiv - Microbiology 2020Quote: ... which were destained and overnight-digested with trypsin (1 μg/mL, sequencing grade, Promega), before MS analysis of the peptide mixes ...
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Toxoplasma GRA15 limits parasite growth in IFNγ-activated fibroblasts through TRAF ubiquitin ligasesbioRxiv - Immunology 2020Quote: ... culture supernatants were removed and 1× lysis buffer was added (Luciferase assay system, Promega) to the cells in the wells ...
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bioRxiv - Bioengineering 2021Quote: ... The bottom of the chamber was coated with 10 μg ml−1 streptavidin (Promega) overnight at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... after which 25 µL assay buffer supplemented with 1:25 furimazine substrate (Promega, N246A) and 1:250 goat anti-rabbit Alexa Fluor 594 was added ...
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bioRxiv - Microbiology 2022Quote: ... 1 µL of this reaction was diluted in 100uL of passive lysis buffer (Promega). Following this ...
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bioRxiv - Molecular Biology 2022Quote: LNCaP/Luc or transfected COS-1 cells were lysed with Reporter Lysis Buffer (Promega). Luciferase activity was assayed using the Steadylite Plus kit (PerkinElmer) ...
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bioRxiv - Cell Biology 2022Quote: ... RNA (1 μg) was reverse transcribed with ImProm-II Reverse Transcriptase (Promega, Madison, Wisconsin) using oligo(dT ...
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bioRxiv - Biochemistry 2019Quote: ... Enzymatic digestion was performed by addition of 1 μg trypsin (Gold, Promega, Madison USA) in TEAB 100 mM and incubation overnight at 30°C ...
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The absence of C-5 DNA methylation in Leishmania donovani allows DNA enrichment from complex samplesbioRxiv - Molecular Biology 2020Quote: ... we used a 1/1500 artificial mix of promastigote DNA and human DNA (Promega) to reflect the median ratio found in clinical samples ...
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bioRxiv - Pathology 2021Quote: ... and anti-rabbit IgG antibody coupled to horseradish peroxidase (Promega France, W401B, 1/2500) and revealed with the Western Lightning chemiluminescence reagent Plus (PerkinElmer Life Sciences) ...
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bioRxiv - Microbiology 2020Quote: ... for 1 h at 55°C then with 200 μg/mL proteinase K (Promega) for 1 h at 55°C ...
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bioRxiv - Systems Biology 2019Quote: ... Cells were then lysed using 1× Passive Lysis Buffer as per manufacturer’s protocol (Promega). Protein concentration was then determined via Bradford assay ...
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bioRxiv - Molecular Biology 2019Quote: ... The final reaction volume contained 1-fold concentrated T4 ligase buffer (Promega, Madison, US). Prepared reaction mixtures (ligase buffer ...
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bioRxiv - Genomics 2021Quote: ... Samples were then treated for 1 hour at 37°C with RQ1 DNase (Promega) to remove possible contamination by DNA ...
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bioRxiv - Biochemistry 2021Quote: ... proteins were digested overnight at 37 °C using trypsin (1:50, w/w, Promega). Peptides were extracted in ACN and formic acid and combined extracts were freeze-dried ...
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bioRxiv - Genomics 2020Quote: ... 0.2 μL GoScript™ RT Mix for 1-Step RT-qPCR (Promega, Madison, WI), 0.75 μL primer/probe sets for either N1 ...
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bioRxiv - Cell Biology 2020Quote: ... pH 7.5) containing 1% Tween20 and 100 U/ml RNasin Plus RNase Inhibitor (Promega). After centrifugation at 15,000 g for 10 min at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... and Dad13 constructs were detected with using mouse-anti-β-galactosidase (1:1000, Promega) and rabbit-anti-β-galactosidase (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... Plasmid constructs (pCAG nHA GPC4, see Table 1) were transfected with Fugene (Promega E2691)/ opti-MEM mixture according to manufacturer protocols ...
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bioRxiv - Genomics 2021Quote: ... cDNA was synthesized from 1 μg RNA using M-MLV reverse transcriptase (9PIM170, Promega) and Oligo(dT)15 primers (C110A ...
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bioRxiv - Cancer Biology 2021Quote: ... or at 25°C with Glu-C (Promega; 1:20 protease:protein w/w ratio) overnight ...
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bioRxiv - Developmental Biology 2021Quote: ... 50 μl of 0.5M TEAB and trypsin (Promega, enzyme: protein ratio of 1:50) were added to the membrane ...
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bioRxiv - Microbiology 2021Quote: ... and digested with sequencing grade trypsin (Promega, 1:50 trypsin- to-protein [wt/wt]) (Yang et al. ...
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bioRxiv - Plant Biology 2021Quote: ... The following components were added: (1) M-MLV reverse transcriptase (Promega, Cat. No. M1701), 1 mM dNTP mix ...
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bioRxiv - Bioengineering 2022Quote: ... cells in each well were lysed in 30 μl 1× passive lysis buffer (Promega). 10 μl per well of lysate was assayed using the Dual Luciferase Assay kit (Promega).
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bioRxiv - Genetics 2022Quote: Type-1 quantitative bioluminescence resonance energy transfer (qBRET) experiments utilised the NanoBRET platform (Promega) and followed the general type-1 qBRET protocol reported by Felce et al.25 The assay was performed in HEK293 cells transiently expressing POPDC1 and POPDC2 constructs which possessed a C-terminal NanoLuc luciferase or HaloTag domains ...
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bioRxiv - Plant Biology 2022Quote: ... The digestion solution was diluted to <1 M urea upon addition of trypsin (Promega, V511A ...
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bioRxiv - Microbiology 2022Quote: ... 0.1-1 µg of RNA was reversed transcribed using M-MLV Reverse Transcriptase (Promega) with dNTPs ...
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bioRxiv - Immunology 2022Quote: ... 1-2 µg RNA was reverse transcribed using AMV Reverse Transcriptase (Promega, Madison, WI). Primers were purchased from Integrated DNA Technologies (IDT ...
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bioRxiv - Microbiology 2022Quote: ... cDNAs were synthesized from 1 μg of total RNA using AMV reverse transcriptase (Promega) and used directly for quantitative PCR (qPCR ...
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bioRxiv - Microbiology 2022Quote: ... Reactions were performed with the GoTaq® Probe 1-Step RT-qPCR System (Promega) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... RT-qPCR was performed using the GoTaq® 1-Step RT-qPCR System (Promega) with an input of 50 ng RNA ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then washed in fresh C+Y medium and 1 % NanoGlo substrate (Promega) was added and luminescence was measured 20 times every 1 min in a plate reader (VarioSkan luminometer ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were digested overnight at 37 °C with 1 μg Typsin/LysC mix (Promega) and 0.01% ProteaseMAX (Promega) ...
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bioRxiv - Physiology 2022Quote: ... samples were digested overnight with trypsin (Promega; Cat# V5113; 50:1 w/w, protein:enzyme)at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... Protein enzymatic cleavage was carried out with trypsin (1:20, w/w; Promega, V5280) at 37 °C for 16 h ...
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bioRxiv - Microbiology 2022Quote: ... and the RNA stored in 1 μL of RNasin® Ribonuclease Inhibitor (Promega Corporation). RNA was quantified with the Qubit™ RNA HS Assay Kit (Invitrogen) ...
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bioRxiv - Immunology 2022Quote: ... Caspase-1 activity in supernatants was quantified using a GloMax Navigator (Promega, Madison, WI). To assess the impact of caspase-1 on MV-induced IL-1β production ...
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bioRxiv - Plant Biology 2022Quote: ... cDNA was synthesized from 1 µg of RNA using M-MLV Reverse Transcriptase (Promega) and oligo d(T)15 following the manufacturer’s instructions ...