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Citations for Promega :
351 - 372 of 372 citations for Streptavidin magnetic beads since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Rinsed beads were resuspended in 15 µL of 50 mM HEPES buffer (pH 8) supplemented with trypsin/LysC mix (Promega) at an enzyme-to-protein ratio of 1:25 (w/w ...
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bioRxiv - Molecular Biology 2021Quote: ... Iso-Seq libraries were prepared following the Iso-Seq™ Express Template Preparation protocol (PN 101-763-800, Pacific Biosciences, CA) and size selected using ProNex beads (Promega) with a bead-to-DNA ratio of 0.95 to incorporate transcripts < 2kb ...
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CREB5 reprograms nuclear interactions to promote resistance to androgen receptor targeting therapiesbioRxiv - Cancer Biology 2021Quote: ... Rehydration of the beads was with 50 mM ammonium bicarbonate solution containing 12.5 ng/µl modified sequencing-grade trypsin (Promega, Madison, WI) at 4°C ...
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bioRxiv - Neuroscience 2022Quote: Magnetic affinity beads were covered with 40 µl of 40 ng/µl sequence grade trypsin in 50mM ammonium bicarbonate pH8 buffer (Promega V5111). The beads were placed in an incubator at 37C overnight ...
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bioRxiv - Genetics 2022Quote: ... the collected lysates (5% of which were collected as input control) were incubated with 100 μM TASQs (or 100 μM biotin as control) and 90 μg of MagneSphere® beads (Promega) for 2 h at 4°C ...
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bioRxiv - Immunology 2024Quote: DNA was extracted from 200 mg of each fecal sample via bead beating and column purification using a Maxwell 16 MDx Instrument (Promega, USA), as previously described (Sikder ...
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bioRxiv - Molecular Biology 2023Quote: ... Beads were washed 5x with lysis buffer containing 150 mM NaCl and resuspended in 500 µl HDAC-Glo buffer (Promega, #G648A). Beads were serially diluted with HDAC-Glo buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were then pelleted and disrupted by glass bead agitation at 4°C in 1x Passive Lysis Buffer provided in the Dual-Luciferase Reporter Assay System (Promega #E1910). Extracts were clarified by centrifugation ...
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bioRxiv - Microbiology 2021Quote: DNA was extracted by mechanical disruption (repeated bead-beating) and purified in a Maxwell RSC instrument (Promega Benelux BV, Leiden, The Netherlands). The Maxwell RSC Blood DNA extraction kit was according to manufacturer’s instructions with several modifications ...
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bioRxiv - Neuroscience 2022Quote: ... we transferred the beads to 100 µL 50 mM Tris-HCl buffer with mixed with 1 µg trypsin/Lys-C (Promega Cat# V5073) per replicate ...
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bioRxiv - Molecular Biology 2022Quote: ... Pelleted cells were disrupted by glass beads agitation at 4°C in 1x Passive Lysis Buffer provided in the Dual-Luciferase® Reporter Assay System (Promega, #E1910). Extracts were clarified by centrifugation ...
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bioRxiv - Molecular Biology 2024Quote: ... tissue homogenization was carried out by bead beating and immediately followed by RNA extraction using the Maxwell® RSC simplyRNA Tissue Kit (Promega AS1340) as per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... The clear supernatant was incubated overnight at 4°C with 100 μl of prewashed Magne® HaloTag® Beads (Promega, WI, USA). Post-incubation ...
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bioRxiv - Microbiology 2023Quote: ... Sample digestion was carried out by resuspending beads in 1 µL of trypsin solution (0.2 µg trypsin in Promega V5111 resuspension buffer) and 19 µL 50 mM ammonium bicarbonate and incubating at 37 °C with agitation for 18 hours ...
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bioRxiv - Physiology 2020Quote: ... The beads were resuspended in 10 μl of reverse transcriptase mix (100□U, SuperScript II, Invitrogen; 10 U, RNAsin, Promega, Madison, Wisconsin, USA), 1□×□Superscript II First-Strand Buffer ...
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bioRxiv - Plant Biology 2020Quote: ... approximately 0.6 mg IgG-enriched proteins were first digested on the beads with mass spectrometry-grade Trypsin/Lys- C Mix (Promega, Madison, WI, USA) at a 1:50 (enzyme/substrate ...
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bioRxiv - Microbiology 2021Quote: ... The kinase activity assay was performed with 4 µl beads in a 384-well plate using the ADP-Glo™ Kinase Assay (Promega, Madison, WI). The luminescence of each well was read using a Synergy™ H1 Hybrid Multi-Mode Microplate Reader (BioTek Instruments ...
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bioRxiv - Microbiology 2020Quote: Faecal DNA was extracted and purified using a combination of repeated bead-beating (method 5) (Costea et al, 2017) and the Maxwell 16 Tissue LEV Total RNA Purification Kit (Promega, Maddison, WI, USA), with STAR (Stool transport and recovery ...
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bioRxiv - Cell Biology 2024Quote: ... beads were equilibrated in 50 mM of ammonium bicarbonate with 2 washes followed by on-bead digestion with trypsin (800 ng; Promega cat. nr. V5280) overnight at 37 °C ...
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bioRxiv - Synthetic Biology 2023Quote: Casp3-containing matrix consisted of an N- and C-terminally HaloTag®-ed Casp3 coupled to Magne® HaloTag beads (Promega, Cat.-No. G7281). To ensure binding on both termini ...
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bioRxiv - Biochemistry 2024Quote: ... DNA was isolated from the fecal slurries using a bead-beating procedure coupled with the customized MaxWell 16 Tissue LEV Total RNA Purification Kit (XAS1220; Promega Biotech AB, Stockholm, Sweden). DNA isolates underwent triplicate polymerase chain reaction (PCR ...
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bioRxiv - Cancer Biology 2022Quote: ... the beads were washed in the ABC buffer (50mM ammonium bicarbonate, pH 8.3) and proteins digested on beads with TPCK-trypsin (Promega, Madison, WI, 16 hours at 37 °C). The supernatant containing the tryptic peptides was collected and lyophilized ...