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Citations for Promega :
301 - 350 of 372 citations for Streptavidin magnetic beads since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... The supernatant without beads was transferred to a new tube and digested with 150 ng trypsin (Trypsin Gold, Promega) in 1.5 µL 50 mM ammonium bicarbonate at 37°C for 5 hours ...
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bioRxiv - Genetics 2022Quote: ... Protein-bound beads were washed in 80% ethanol and proteins were digested off the beads by addition of 0.8ug sequencing grade modified trypsin (Promega) in 100mM Ammonium bicarbonate ...
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bioRxiv - Cancer Biology 2022Quote: ... The beads were finally added with 2.5 μl of 200 ng/µl Trypsin/Lys-C Mix (Cat. No. V5073, Promega) at 37°C with shaking overnight ...
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bioRxiv - Genetics 2020Quote: ... beads were resuspended in 100 μL of ABC buffer and digested by adding 0.2 μg of trypsine/LysC (Promega) in 100 µL of ABC buffer for 1 hour at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... and digested on-bead using filter-aided sample preparation47 with sequencing grade modified porcine trypsin (Cat. No. V5111, Promega). Nano-liquid chromatography tandem mass spectrometry (Nano-LC/MS/MS ...
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bioRxiv - Cell Biology 2023Quote: ... and digested on-bead using filter-aided sample preparation (Wiśniewski, Zougman, Nagaraj, & Mann, 2009) with sequencing grade modified porcine trypsin (Promega). Tryptic peptides were separated by reverse phase XSelect CSH C18 2.5 µm resin (Waters ...
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bioRxiv - Cell Biology 2024Quote: ... Each sample was digested on beads in 100 µl of 50 mM ammonium bicarbonate with 1 µg trypsin (Promega) in spin cap tubes sitting in fresh mass-spectrometry grade Eppendorf tubes ...
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bioRxiv - Cancer Biology 2023Quote: ... Beads were washed 6 times with 50 mM ammonium bicarbonate and then treated with TPCK-treated modified trypsin (Promega) for 16 hours at 37°C on an end-over-end rotator ...
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bioRxiv - Cancer Biology 2023Quote: ... beads were reconstituted in 5 μL 50 mM HEPES pH 8.0 buffer containing trypsin/rLys-C enzyme mix (Promega) at a 1:25 enzyme to protein ratio ...
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bioRxiv - Molecular Biology 2022Quote: ... beads were incubated in 400 μl 50 mM ammonium bicarbonate containing 4 μl of sequencing-grade modified trypsin (Promega) at 37 °C overnight ...
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bioRxiv - Microbiology 2024Quote: ... Protein digestion was done overnight at 37°C on-bead in 100 µL of 25 mM ammonium bicarbonate containing 0.5 µg of MS grade Trypsin/LysC (Promega) (enzyme/protein ratio of 1:20 (w/w)) ...
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bioRxiv - Systems Biology 2024Quote: ... The rinsed beads were resuspended in 115 μL of 50 mM HEPES buffer (pH 8.0) supplemented with trypsin/LysC (Promega) at an enzyme-to-protein ratio of 1:25 (w/w ...
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bioRxiv - Biochemistry 2024Quote: ... was added to 0.5 mL 1% bovine serum albumin (BSA) in IP/lysis buffer (Pierce, 87788) and incubated with MagneHis beads (Promega, 32 µL suspension ...
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bioRxiv - Cell Biology 2024Quote: ... and digested on-bead using a filter-aided sample preparation (Wiśniewski et al., 2009) with sequencing grade-modified porcine trypsin (Promega). Tryptic peptides were then separated by reverse phase XSelect CSH C18 2.5 um resin (Waters ...
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bioRxiv - Cell Biology 2024Quote: ... and digested on-bead using a filter-aided sample preparation (Wiśniewski et al., 2009) with sequencing grade-modified porcine trypsin (Promega). Tryptic peptides were trapped and eluted on 3.5um CSH C18 resin (Waters ...
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bioRxiv - Microbiology 2022Quote: ... Indexed libraries were then cleaned using AMPure XP beads and quantified on the Quantus Fluorometer using the QuantiFluor ONE dsDNA System (Promega). Amplicons were purity-checked and sized on a TapeStation using D1000 ScreenTape System (Agilent) ...
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bioRxiv - Neuroscience 2021Quote: IP and pull-down probes of DG NSCs were subjected to on-bead digestion (Hubner et al., 2010) by trypsin (5 µg/ml, Promega) in 1.6 M Urea / 0.1 M Ammonium bicarbonate buffer at 27 C for 30 minutes ...
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bioRxiv - Genomics 2020Quote: ... A double-stranded DNA adapter with a T-overhang was ligated for 1 h at 20°C and the resulting products were bead purified (ProNex, Promega), eluted and then split into two identical aliquots ...
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bioRxiv - Cell Biology 2021Quote: ... which were washed with a series of harsh washes before cross-linker reversion and on bead digest with Trypsin Lys-C (Promega). Peptides were then desalted and prepared for mass spectrometry ...
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bioRxiv - Cell Biology 2021Quote: ... Washed beads were re- suspended in 150 µl digestion buffer and incubated for 4 hours with 1 µg trypsin (Promega) at 37 °C ...
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bioRxiv - Cell Biology 2021Quote: ... On-bead digestion was performed by overnight incubation at 37°C with 2 μg of mass spectrometry grade trypsin (Promega). The following morning an additional 2 μg of trypsin was added to each sample and incubated at 37°C for 4-6 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... mix was used at a concentration of 100 μg/μl for overnight “on-bead” digestion using LysC-trypsin mix (Promega)2,3 ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins on beads were digested overnight at 37°C by sequencing grade trypsin (12.5 μg/ml; Promega Madison, Wi, USA) in 20 μl of NH4HCO3 25 mmol/l ...
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bioRxiv - Genomics 2022Quote: ... Then the gel beads were washed with DPBS three times and 200 μL of proteinase K solution (1 mg/mL proteinase K [Promega, Madison ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was eluted from the beads using the elution buffer (1% SDS and 100 mM NaHCO3) and purified by PCR purification kit (Promega). The final products were analyzed by quantitative real-time PCR with the primers listed below:
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bioRxiv - Cell Biology 2020Quote: ... and the proteins were on-bead digested using 5 µl of Sequencing Grade Trypsin (100 ng/µl in 10 mM HCl, Promega). The digestion was carried out at 37°C over night ...
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bioRxiv - Pathology 2020Quote: The cellular ATP levels were quantified in 100 μL ATP assay buffer or from 20-30 mg wound skin harvested in 200 μL assay buffer and lysed with the bead shocker following the manufacturer’s instructions (CellTiter-Glo luminescent ATP assay kit, Promega, Madison). The protein content from identical treated cells or tissues were determined by BCA Protein Assay kit for normalization (Young ...
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bioRxiv - Molecular Biology 2020Quote: ... and protein complexes were directly digested on-beads by the addition of 2 μg of a Trypsin/Lys-C mixture (Promega). Peptides were isolated on C18 resin tips according to the manufacturer’s instructions (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Antibodies were obtained in carrier/ protein-free buffer or were purified using the Magne Protein A or G Beads (Promega) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... enriched proteins were digested at 37 °C overnight on agarose beads in RapiGest SF solution (Waters, 186001861) supplemented with 0.75 μg trypsin (Promega, V511A). The reaction was stopped using 1% TFA (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: Proteins bound to beads were reduced with 2 mM DTT in 2 M urea buffer and sequencing grade trypsin (Promega) was added to a final concentration of 5 ng/μl ...
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bioRxiv - Microbiology 2022Quote: ... 120 μg of fragmented total RNA was added to the antibody-bound beads in 250 μl IP buffer supplemented with RNasin Plus RNase inhibitor (PRN2615, Promega), and the mixture was incubated at 4 °C for 2 h ...
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bioRxiv - Neuroscience 2023Quote: ... the washed beads were re-suspended in 150 µl trypsin digestion buffer and incubated for 4 hours with 1 µg trypsin (Promega) at 37 °C ...
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bioRxiv - Neuroscience 2023Quote: ... PCR-amplified libraries were purified and size selected for fragments of 200-250bp using the Pronex paramegnetic beads (Promega # NG2001) according to manufacturer instructions ...
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bioRxiv - Neuroscience 2022Quote: ... The supernatant was removed and tryptic peptides were eluted from the beads by overnight digestion in 50 µl trypsin solution (V5111, Promega, 0.01 µg/µl in 100 mM ammonium bicarbonate ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were processed using the SP3 protocol (Hughes et al.2014) and on-bead digested with trypsin (sequencing grade, Promega), which was added in an enzyme to protein ratio 1:50 for overnight digestion at 37°C ...
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bioRxiv - Biochemistry 2024Quote: ... beads were resuspended in 100 μL of 50 mM TEAB and enzymatically digested overnight using sequencing-grade trypsin (Promega, V5111). Digested peptides were eluted through centrifugation and labeled using commercially available TMTsixplex tags (ThermoFisher ...
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bioRxiv - Developmental Biology 2023Quote: ... Sample cleanup was performed using the SP3 method 83 and on-bead digestion was performed overnight using Trypsin/Lys-C (Promega). Eluted tryptic peptides from SP3 beads were pre-fractionated by high pH reverse phase chromatography into 96 individual fractions using a 64-minute gradient from 1% to 50% solvent B (A ...
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bioRxiv - Microbiology 2023Quote: ... VSV-G agarose beads and bound proteins were then treated with 10 μL of 10 μg/μL sequence grade trypsin (Promega) for 16 h at 37℃ with mild shaking ...
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bioRxiv - Biochemistry 2023Quote: ... beads were resuspended in 100 μL of 50 mM TEAB and enzymatically digested overnight using sequencing-grade trypsin (Promega, V5111). Digested peptides were eluted through centrifugation and labeled using commercially available TMTsixplex tags (ThermoFisher ...
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bioRxiv - Developmental Biology 2022Quote: ... Proteins were on-bead digested using 5 μl of Sequencing Grade Trypsin (100 ng/μl in 10 mM HCl, Promega). The digestion was carried out in a microwave instrument (Discover System ...
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bioRxiv - Biochemistry 2023Quote: The binding affinity of Ndel1 constructs for dynein and Lis1 was determined by coupling Ndel1 to 25 µL of Magne HaloTag Beads (Promega) in 2 mL Protein Lo Bind Tubes (Eppendorf ...
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bioRxiv - Biochemistry 2023Quote: ... Selected gel bands that did not bind to the 4’-methylgenistein beads were subjected to in-gel digestion with trypsin (MS grade; Promega). Peptide fragments were analyzed using a Q-TOF/MS ...
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bioRxiv - Plant Biology 2024Quote: ... PCR amplification of WLV was done using PuReTag Ready-to-Go PCR beads (Illustra) or Go Taq DNA polymerase (Promega) according to the manufacturers’ instructions and WLV-specific primer pairs 4194+4195 ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins bound to magnetic beads were washed twice with 100 µL of 25 mM NH4HCO3 and on-beads digested with 200 ng of trypsine/LysC (Promega) for 1 hour in 100 µL of 25 mM NH4HCO3 at room temperature ...
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bioRxiv - Immunology 2024Quote: Migrated or beads-separated RAG-/- OT-I T cells were processed using the ReliaPrepTM RNA Cell Miniprep System (Promega #Z6011) and total RNA was isolated following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... the cDNA library is pre-amplified in a first PCR (6 cycles) followed by size selection with ProNex beads (Promega) to remove short cDNAs and primer dimers ...
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bioRxiv - Molecular Biology 2024Quote: Halo-tagged fusion proteins (200 pmol) were bound to 10 μL of Magne® HaloTag® Beads (20% slurry, Promega), followed by washing ...
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bioRxiv - Genetics 2024Quote: Conditioned media from NL-WNT2B-expressing cells were collected after 48h transfection with plasmids encoding HT7-tagged carriers or entire GPC ectodomains and were captured on HaloLink beads (Promega) as previously described(65) ...
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bioRxiv - Systems Biology 2024Quote: ... On-bead protein digestion was performed overnight in 50 mM triethylammonium bicarbonate (TEAB) pH 9 with mass spectrometry-grade trypsin (Promega) and LysC (FUJIFILM ...