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Citations for Promega :
351 - 400 of 1308 citations for Rabbit Anti Sheep IgG H+L Alkaline Phosphatase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... goat α-mouse IgG antibodies (Promega) and donkey α-goat IgG (Abcam ...
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bioRxiv - Microbiology 2023Quote: ... goat α-mouse IgG antibodies (Promega) and donkey α-goat IgG (Abcam ...
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bioRxiv - Microbiology 2023Quote: ... goat α-mouse IgG antibodies (Promega) and donkey α-goat IgG (Abcam ...
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bioRxiv - Neuroscience 2021Quote: ... Recombinant viral DNA was extracted under alkaline conditions, followed by ethanol precipitation (Birnboim and Doly, 1979) and used for transfection into insect cells (Fugene HD, Promega). Baculovirus recombinant for COL6A3WT or COL6A3ΔC4 were propagated in Sf21 cells and supernatant (SN ...
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bioRxiv - Immunology 2023Quote: ... 1 mmol/L dithiothreitol (V315A, Promega), 5 mmol/L β-glycerophosphate (G6376 ...
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bioRxiv - Biochemistry 2019Quote: ... Media was replaced at 24 h post-transfection and cells were lysed at 48 h post-transfection using 200 ml passive lysis buffer (Promega) followed by freezing at −80 °C ...
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bioRxiv - Biochemistry 2019Quote: ... Media was replaced at 24 h post-transfection and cells were lysed at 48 h post-transfection using 200 ml passive lysis buffer (Promega). FLuc and RLuc activity was measured using a dual-luciferase reporter assay system (Promega) ...
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bioRxiv - Immunology 2021Quote: ... JEG-3 viability after 1 h salubrinal pretreatment followed by 48 h ZIKV infection was measured by CellTiter-Glo Assay (Promega) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were digested for 2 h at 37°C with Lys-C and 16 h at 37°C with Trypsin Gold (both Promega). For TMT 6-plex labeling (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: NAD(P)+ and NAD(P)H concentrations were quantified with NAD(P)- and NAD(P)H-Glo assay kits (Promega) according to manufacturer instructions ...
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bioRxiv - Genomics 2020Quote: ... and M-MLV Reverse Transcriptase RNase H (Promega). cDNA was amplified using Taqman Universal Master Mix with UNG (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... the samples were treated with RNase H (Promega) for 30 min at 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... reverse transcribed using MMLV reverse transcriptase (H-)(Promega) at 42°C for 1 hour and buffer exchanged to TBS-T using sephadex G-25 ...
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bioRxiv - Microbiology 2022Quote: ... using MMLV reverse transcriptase RNase H Minus (Promega). Purified resuspended cDNAs were sequenced on a CEQ 8000 capillary electrophoresis sequencer (9+) ...
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bioRxiv - Genetics 2022Quote: Endoglycosidase H (EndoH) (Promega V4871, 500 U/μL) was used to deglycosylate Ina1-GFP-containing samples ...
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bioRxiv - Biochemistry 2022Quote: ... the proteins were deglycosylated by Endo-H (Promega) followed by PNGaseF (Promega ...
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bioRxiv - Neuroscience 2023Quote: ... cells were incubated with 10µM Coelenterazine-h (Promega) in the dark for 2 hours before recording luminescence in a plate reader (Optima FLUOStar ...
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bioRxiv - Plant Biology 2020Quote: ... Membranes were washed 3 × 10 min in TBS-T (0.1%) before 1hr incubation in secondary antibodies (anti-rabbit or anti-mouse HRP or AP conjugate (Promega; 1: 5.000)) ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were washed with TBS-T and incubated for 60 min at room temperature with an anti-mouse or anti-rabbit horseradish peroxidase (HRP)-conjugated secondary antibody (Promega, Germany) at dilutions of 1:5000 ...
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bioRxiv - Plant Biology 2022Quote: ... Membranes were washed 3 × 10 min in TBS-T (0.1%) before 1hr incubation in secondary antibodies (anti-rabbit or anti-mouse HRP or AP conjugate (Promega; 1: 5.000)) ...
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bioRxiv - Cancer Biology 2023Quote: ... Secondary HRP-conjugated antibodies were added at a dilution of 1:10,000 in 5% milk in TBST and incubated for 60 minutes at room temperature (Anti-Mouse: Promega #W402B; Anti-Rabbit: Promega #W401B). The Pierce SuperSignal West Pico PLUS chemiluminescent substrate (Thermo Scientific #34577 ...
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bioRxiv - Microbiology 2020Quote: ... used at a 1:10,000 dilution and the secondary antibody was an Anti-Mouse IgG HRP conjugate antibody (Promega, #W4021) used at a 1:10,000 dilution.
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bioRxiv - Microbiology 2020Quote: ... the plates were washed with PBS-T five times and added with a 1:5000 dilution of HRP-conjugated goat anti-mouse IgG antibody (Promega) in blocking buffer at 37 °C for 45 min ...
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bioRxiv - Microbiology 2020Quote: ... incubating and chromogenic reaction generating were similar to the ACE2 assay instead of that primary antibody binding to antigen was not required and that HRP-conjugated goat anti-human IgG antibody (Promega) would be used as the secondary antibody to detect binding of CB6 antibody to the antigens.
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bioRxiv - Neuroscience 2023Quote: ... Membranes were rinsed 5 times for 8 min in PBS-tween 0.2% and incubated with secondary antibodies for 2 h at room temperature under agitation (HRP anti-mouse, 1:10000, Promega cat. # W4021). Membranes were further rinsed 5 times for 8 min in PBS-tween 0.2% ...
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bioRxiv - Biochemistry 2022Quote: ... secondary antibody staining was performed for 1 h at room temperature using antirabbit HRP or anti-mouse HRP secondary antibodies (Promega,1:10,000) in blocking buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by the addition of 5-μL Endo-H reaction buffer and 5-μL Endo-H (Promega Cat#PRV4875, 2,500-U). Deglycosylation proceeded for 4-hours at 37°C ...
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bioRxiv - Microbiology 2019Quote: ... Relative growth was determined using luciferase levels measured at 0 h (initial setup values) and after 72 h using the GloMax® Discover Multimode Microplate Reader (Promega). Luminescence values were normalized to samples treated with a lethal dose of chloroquine (200 nM ...
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bioRxiv - Developmental Biology 2019Quote: ... 96 and 120 h after transfection and 4 h after adding 20 µl of CellTiter 96® AQ One Solution Reagent (Promega) to each well containing transfected TCam-2 cells.
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bioRxiv - Microbiology 2020Quote: ... Cells were treated 8 h post-transfection with or without IFN-α (1000 U/ml) before lysis 16 h later using Passive Lysis Buffer (Promega). Firefly and Renilla luciferase activity was then determined in a dual luciferase assay ...
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bioRxiv - Cancer Biology 2021Quote: ... The viability of the cultures was determined 24 h and 48 h after EV treatments by using the CellTiter-Fluor cell viability assay kit from Promega (G6080) following manufacturers instructions ...
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bioRxiv - Microbiology 2022Quote: ... The samples were incubated at 30 °C and luminescence was quantified after 2 h and 4 h post infection (20 μl sample plus 20 μl substrate, Nano-Glo® Luciferase Assay System, Promega). The sample was carefully removed from the upper part of the tube without disturbing the sedimented erythrocytes in the blood samples.
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bioRxiv - Microbiology 2023Quote: ... The presence of glycosylated proteins in the cell lysates was assessed using Promega Endoglycosidase H (Endo H) assay reagents (Promega V4871). Briefly ...
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bioRxiv - Physiology 2022Quote: ... followed by 1:2500 goat anti-rabbit HRP-conjugated secondary (catalog #: W4011; Promega, Madison, WI). p-ATP1A1Y10 protein bands were visualized with SuperSignal™ West Pico Plus (SuperSignal Pico ...
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bioRxiv - Immunology 2021Quote: PP2A activity in cell lysates was measured using the Phosphatase Assay Kit (Promega). Endogenous free phosphate was removed using the columns ...
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bioRxiv - Genetics 2019Quote: ... fixed with 4% paraformaldehyde and labeled with rabbit polyclonal anti-GFP (Minotech Biotechnology) and mouse anti-β-galactosidase (Promega Cat# Z3781, RRID:AB_430877) primary antibodies ...
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bioRxiv - Immunology 2020Quote: ... IgG were deglycosylated using PNGase F (Promega) with 0.05 U of PNGase/μg of IgG ...
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bioRxiv - Immunology 2021Quote: ... and bacterial bioluminescence was measured every 24 h for up to 120 h with a GloMax Multi Detection System (Promega, Madison, WI). For the M.tb replication rate experiment ...
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bioRxiv - Neuroscience 2022Quote: ... 10µl RNasin RNase-inhibitor (40U/l, Promega) were added ...
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bioRxiv - Microbiology 2019Quote: ... cDNA was synthesized using M-MLV RT (H–) (Promega) and U3 antisense primer 5’-TGTGTGGTAGATCCACATCG-3’ ...
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bioRxiv - Immunology 2020Quote: ... and M-MLV RT RNAse (H-) Point Mutant (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... and coelenterazine h was purchased from Promega (Madison, WI). 7-ethoxyresorufin was purchased from Anaspec (Fremont ...
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bioRxiv - Biophysics 2021Quote: ... except that the substrate used was coelenterazine h (Promega, 5μM final concentration in drug buffer ...
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bioRxiv - Microbiology 2024Quote: ... and incubated with coelenterazine-h (Promega, Madison, WI, USA) at a final concentration of 2.5 nM ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 10 µL coelenterazine h (Promega, 5 µM final concentration) was added ...
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bioRxiv - Cell Biology 2024Quote: ... for 3 h and with trypsin (1:25, Promega) for 16 h both at 37°C ...
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bioRxiv - Developmental Biology 2020Quote: ... Primary antibodies used were: rabbit and mouse anti-βGal (1/1000; Promega and MP Biomedicals, respectiveley), rat anti-Ser (1/1000 ...
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bioRxiv - Genomics 2022Quote: ... Sections were then incubated with a horseradish peroxidase-labeled anti-rabbit antibody (1:300, Promega, W401B) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... supernatant and pellet fractions were spotted onto nitrocellulose and blotted with rabbit anti-HaloTag pAB (Promega). For microscopic examination ...
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bioRxiv - Plant Biology 2023Quote: ... the membrane was incubated with a horseradish peroxidase (HPR) conjugated anti-rabbit antibody (Promega, Walldorf, Germany) diluted 1:10.000 in PBS-T with 3% milk powder for 1 h ...