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Citations for Promega :
451 - 500 of 1308 citations for Rabbit Anti Sheep IgG H+L Alkaline Phosphatase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... 48-55 h after infection Bright-Glo Luciferase Assay System (Promega, E2610) was used to measure luciferase activity ...
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bioRxiv - Microbiology 2022Quote: ... 24 h post transfection the Dual-Luciferase® Reporter Assay System (Promega) was used according to the manufacturer’s manual ...
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bioRxiv - Neuroscience 2023Quote: ... Cellular NAD(H) levels were measured using NAD/NADH-Glo assay (Promega). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... After 16 h cells were lysed in 1x passive lysis buffer (Promega) and firefly luciferase activity was measured after addition of firefly substrate-containing buffer (20 mM Tricine ...
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bioRxiv - Microbiology 2022Quote: ... As secondary antibodies α-mouse IgG HRP conjugate (Promega W4021) or α-rabbit IgG HRP conjugate (Cell Signaling #7074 ...
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bioRxiv - Biochemistry 2020Quote: hnRNP L and SETD2 human ORF were procured from Promega. Deletion mutants of hnRNP L and SETD2 were constructed by PCR (Phusion polymerase ...
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bioRxiv - Immunology 2023Quote: ... 50 ☐l of Bright-Glo Luciferase reagent (Promega E2620) was added to each well ...
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bioRxiv - Microbiology 2023Quote: ... Rabbit Reticulocyte Lysate (Promega), ATP regeneration system (0.5 mM ATP [Wako]) ...
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bioRxiv - Cell Biology 2021Quote: ... Anti-mouse HRP conjugate (W4021, 1:10,000) and Anti-Rabbit HRP Conjugate (W4011, 1:10,000) were purchased from Promega (Madison, WI). Anti-COPI antibody was a gift from Charles Barlowe (Dartmouth Univ ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were resolved in reducing and non-reducing SDS-PAGE gels and membranes were incubated overnight at 4°C with the following antibodies: rabbit polyclonal anti-human p75 intracellular domain (1:1000, Promega); mouse monoclonal anti-HA (1:2000 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... before re-suspending in 50 μl flow buffer containing anti-Nluc polyclonal primary antibody raised in rabbit (Kindly gifted by Promega) at 1:100 dilution and incubated at room temperature for 1 hour ...
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bioRxiv - Cell Biology 2020Quote: ... using one of the following antibodies against (all dilutions in PBS): HaloTag® polyclonal anti-rabbit (G928A, Promega, 1:100); ACTN2 monoclonal anti-mouse (EA-53 Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... a rabbit polyclonal antibody was used followed by detection with a goat anti-rabbit secondary antibody conjugated to horseradish peroxidase and developed using a chemiluminescent substrate (Promega). Immunoblot detection of 6xHIS and 2A peptide tags were determined through anti-Histidine tag (AD1.1.10 ...
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bioRxiv - Developmental Biology 2023Quote: ... The following day membranes were washed 3 times n TBTS for 5 minutes and incubated in secondary antibody (1:2500 anti-Rabbit HRP conjugated, Promega) diluted in blocking buffer for 1 hour at room temperature and washed 3 times with TBTS for 5 minutes at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... IΒΑ1 (1:250, rabbit, Wako, 01919741) and p75NTR (1:1000, rabbit, G3231, Promega). Sections were washed 3×20 min in PBS prior to incubation with secondary antibodies (Alexa Fluor ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were digested by 2 h Trypsin/Lys-C mix incubation (V5073, Promega) and collected ...
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bioRxiv - Molecular Biology 2019Quote: ... and reverse transcribed with Moloney murine leukemia virus reverse transcriptase RNase H(−) (Promega) using oligoT primer ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The enhancer constructs were transfected 24 h after seeding using FuGENE6 (Promega, US) as the transfection reagent ...
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bioRxiv - Microbiology 2020Quote: ... was measured at 72 h post-infection using the Bright-Glo reagent (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... Acquorin was reconstituted by treating the seedlings with coelenterazine h (Promega, Beijing, China) in dark overnight at a final concentration of 10 μM ...
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bioRxiv - Microbiology 2020Quote: ... The product was subsequently reverse-transcribed using RNase H minus reverse transcriptase (Promega) at 42°C for 30 minutes and buffer was exchanged for DDM buffer ...
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bioRxiv - Neuroscience 2022Quote: ... Luciferase activities were assayed 48 h later using the Dual-Glo system (Promega). Firefly/Renilla luciferase ratio was calculated for each condition and referred to as the percentage relative to the baseline activity of pGL3-Fezf2-downstram-luc.
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bioRxiv - Immunology 2021Quote: ... Promega (ADCP FcγRIIa-H Reporter Bioassay, Core Kit, Promega Corporation Catalog number: G9995) respectively ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were recorded 48– 72 h after tetracycline induction or transfection (Fugene (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... After 17-20 h 50 μL/well of One-Glo-EX substrate (Promega) was added to the cells and incubated in the dark for 5-10 min prior reading on a Varioskan LUX plate reader (ThermoFisher) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and incubated at 37 °C for 3 h with Trypsin/Lys-C (Promega) at a 25:1 protein:protease ratio (w/w) ...
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bioRxiv - Microbiology 2022Quote: ... Cells were harvested at 20 h post-transfection with passive lysis buffer (Promega) and a dual luciferase detection assay was conducted upon the lysates (Promega).
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bioRxiv - Developmental Biology 2023Quote: ... pCDNA3 HSV-TK 2xMyc Su(H)VP16 and pRL TK (Promega, Madison, USA). For the Luciferase assay ...
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bioRxiv - Cell Biology 2022Quote: ... and 12 h and GEMs were stained 100 nM of Halo-JF646 (Promega) for 1 h before fixation ...
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bioRxiv - Microbiology 2024Quote: ... was measured at 48 h post-infection using the Bright-Glo reagent (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Secondary -mouse IgG-HRP conjugate (Promega, Mannheim, Germany; 1:4,000 dilution) was used for detection ...
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bioRxiv - Biochemistry 2020Quote: ... CaCl2·2H20 (0.99 g/L)) supplemented with 0.05% Tween 20 (Promega), 2.5 mM DTT ...
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bioRxiv - Cell Biology 2020Quote: ... Mcm2-7 was probed with polyclonal UM174 (gift from Bell lab, MIT - (Chen and Bell, 2011)) and anti-rabbit HRP conjugate secondary (Promega W401B), tubulin was probed with monoclonal anti-tubulin antibody (Sigma T5168 ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were washed thoroughly in PBS-T before incubation with secondary antibodies (anti-rabbit horseradish peroxidase, Promega WB401B 1:2,500 dilution) for 1 h at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... Membranes were washed 3 × 10 min in TBS-T (0.1%) before 1hr incubation in secondary antibodies (anti-rabbit HRP or AP conjugate (Promega; 1: 5.000)) ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were then washed 3x with TBS-T and incubated for 2 hours at room temperature with either anti-rabbit (1:5000, Promega W4011) or anti-mouse HRP (1:5000 ...
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bioRxiv - Biochemistry 2020Quote: Rabbit reticulocyte lysates (RRL, Promega) were used to investigate the inhibition of translation by DPR proteins ...
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bioRxiv - Microbiology 2022Quote: ... and Nluc (rabbit Pab, Promega). Then ...
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bioRxiv - Microbiology 2020Quote: ... and Nluc (rabbit PAb; Promega). A MAb against actin (MAb AC-15 ...
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bioRxiv - Synthetic Biology 2022Quote: ... and rabbit reticulocyte extract (Promega). All reactions were performed according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2019Quote: ... nuclei from HepG2 cells were isolated by incubating cell pellets in 500 μl of a hypotonic buffer for 15 min on ice (20 mM Tris-HCL, pH 7.4, 10 mM NaCl, 3 mM MgCL2, with protease and phosphatase inhibitor cocktail from Promega). Triton X-100 detergent was added to a 0.5% v:v final concentration to lyse the cells followed by centrifugation at 1200 × g for 10 min to pellet nuclei ...
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bioRxiv - Biochemistry 2023Quote: Serine/threonine phosphatase activity assay of PP2A was performed using the artificial p-peptides RRA(pT)VA substrate (Promega) in lysis buffer containing 1 mM MnCl2 at 70 °C 26 ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were pulsed for 4 h with CellTiter 96 Aqueous One solution reagent (Promega). Cell viability was measured by a luminometer ...
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bioRxiv - Developmental Biology 2020Quote: ... Assays were performed 48 h later using the Dual-Glo Luciferase Assay System (Promega). The basal activity of the empty luciferase vector was set as 1.0 ...
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bioRxiv - Molecular Biology 2020Quote: Cells were harvested 24–48 h after transfection/infection in passive lysis buffer (Promega). The samples were prepared according to the manufacturer’s protocol (Promega ...
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bioRxiv - Cancer Biology 2020Quote: ... After 24 h viability of cells was measured using CTG assay (Promega, Madison, USA). The luminescence was read with a PHERAstar FS plate reader (BMG Labtech ...
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bioRxiv - Cancer Biology 2020Quote: ... Plates were incubated for 1 h at RT and luminescence recorded (Glowmax Luminometer, Promega) and then compared to Glucose/Lactate standards to determine relevant concentrations.
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bioRxiv - Neuroscience 2020Quote: ... Proteins were digested for 18 h at 37°C with 0.5 μg trypsin (Promega). After digestion ...
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bioRxiv - Microbiology 2021Quote: ... incubated for 1 h at RT and analysed using the GloMax multidetection system (Promega). Parasitaemia was used to normalise parasite density between samples and replicates ...
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bioRxiv - Microbiology 2020Quote: ... 72 and 120 h post-treatment using the Renilla-Glo luciferase assay system (Promega) and a GloMax Discover multimode microplate reader (Promega) ...