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Citations for Promega :
351 - 400 of 861 citations for Mouse IgG1 Isotype Control Antibody 15H6 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... the luminescence signals of biosensors and renilla luciferase (an internal control reporter) from pGS vectors were measured by Dual-Glo Luciferase Assay System (Promega). The ratio (firefly luciferase/renilla luciferase ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA was isolated from control (NTC) or DDX39B-depleted (Sh3 or Sh5) CD4+ T cells using ReliaPrep RNA Cell Miniprep System (Promega), and treated in-column with DNase I following the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were co-transfected with the plasmid pGL4.17 containing a YB-TATA-based minimal activity promoter and enhancer sequences (GGAA-msats of various lengths, genomic or synthetic alk-SE sequences) and pRL Renilla Luciferase Control Vector (Promega) (plasmid mass ratio of pGL4.17:pRL = 100:1) ...
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bioRxiv - Microbiology 2022Quote: ... Replicon activity was calculated as the ratio of Renilla (subgenomic reporter) to Firefly (co-transfected loading control RNA, cap-dependent translation) using Dual-Luciferase Stop & Glo Reporter Assay System (Promega) and normalized by the same ratio for the control WT replicon ...
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bioRxiv - Molecular Biology 2020Quote: ... HCT116 cells expressing the auxin-responsive F-box protein Oryza sativa TIR1 (OsTIR1) under the control of a Tet promoter were transfected using FuGENE HD (Promega) with a CRISPR/Cas9 plasmid targeting nearby the 1st ATG codon of the RIF1 gene (5’-TCTCCAACAGCGGCGCGAGGggg-3’ ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were transfected in 96-well plates with 98 ng rTetR plasmid and 2 ng pRL Renilla Luciferase control reporter plasmid (Promega) to control for transfection efficiency ...
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bioRxiv - Bioengineering 2019Quote: ... after removal of its original signal peptide and was further fused to Halo-tag derived from HaloTag Control Vector (Promega) at the C-terminus ...
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bioRxiv - Microbiology 2019Quote: ... 293T cells stably expressing HA-tagged PPP2R5B or APOBEC3G were transfected with 200 ng/well control or Vif expression vector in 24-well plates using FuGENE 6 (Promega). After 36 hr ...
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bioRxiv - Neuroscience 2019Quote: ... HEK293T cells were transfected with control or shRNA-containing pFUGW (GFP-expressing) vector plasmids and helper plasmids PAX2 and VSVG using Fugene (Promega). Supernatant was collected 65 hr after transfection ...
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bioRxiv - Immunology 2021Quote: ... luminescence was measured at 18 hours post-treatment with untreated samples included as a control (GloMax Discovery, Promega, Madison, WI). Data was normalized to the untreated control and presented as the average (N=3 ...
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bioRxiv - Immunology 2020Quote: ... HF and MEF cell lines were transfected with 10 ng of the internal control plasmid pTK-Renilla (pRL-TK, Promega) or 60 ng of the reporter plasmid pLUC-NF-κB (R ...
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bioRxiv - Cancer Biology 2019Quote: ... and Jurkat T cells overexpressing hPD-1 and carrying a luciferase reporter gene under the control of Nuclear Factor of Activated T-cells Response Element (NFAT-RE) (hPD-1 Effector Cells, hPD-1 ECs, Promega) were cultured in RPMI-1640 medium (Biowest ...
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bioRxiv - Molecular Biology 2019Quote: ... Virus-like particles (VLPs) containing the GFP control or targeting sgRNAs were generated by transfection of HEK293 cells with FuGene (Promega). VLPs were collected from culture supernatant ...
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bioRxiv - Neuroscience 2021Quote: ... GFP-NPHP2 and GFP-NPHP5 expression vectors were transiently transfected to 293T/17 cells together with pCS2FLAG empty vectors (as a control) or pCS2FLAG-NPHP1 variants using FuGENE HD (Promega), following the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2021Quote: ... cofilin 1 GDVGQTVDDP[pY]ATFVKMLPDK and dock4 LGLDLVPRKE[pY]AMVDPEDISI phosphopeptides and compared these data to a positive control END[pY]INASL (Promega) phosphopeptide studied in the same experiments.
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bioRxiv - Cell Biology 2021Quote: ... Eluted proteins from control HeLa cells with C-terminal mEGFP tag were digested with mass-spectrometry grade Lys-C and trypsin (Promega). Eluted proteins from a cell line lacking full-length Cdc20 (CDC20_M1-fs-M43 ...
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bioRxiv - Molecular Biology 2021Quote: ... The NF-κB reporter plasmid containing five copies of an NF-κB response element fused to the firefly luciferase gene and TK-renilla control plasmid were obtained from Promega. The IFIT1 (ISG56 ...
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bioRxiv - Microbiology 2022Quote: ... Replicon activity was calculated as the ratio of Renilla (subgenomic reporter) to Firefly (co-transfected loading control RNA, cap-dependent translation) using Dual Luciferase Stop & Glo Reporter Assay System (Promega) and normalized by the same ratio for the control wt replicon ...
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bioRxiv - Cancer Biology 2022Quote: ... and a membrane GFP (75 ng as control for transfection) using FuGENE HD transfection reagent (1µg DNA/1µL FuGENE) (Promega, WI, USA). Next ...
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bioRxiv - Developmental Biology 2022Quote: HEK293 cells cultured on 10cm dishes were transfected with 10 μg of pLVTHM or pHAGE2 vectors under the control of an EF1α promoter and containing the wild-type or mutant versions of Oct4 or Sox2 with Fugene6 (Promega) using a low volume protocol (Steffen et al. ...
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bioRxiv - Genomics 2022Quote: ... Firefly (transfection control) and Renilla (target) luciferase activities were sequentially measured using the Dual-Glo Luciferase Assay System (Promega E2920) and detected on a Spectramax M plate reader (Molecular Devices) ...
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bioRxiv - Physiology 2022Quote: ... and 0.5 μg of reference plasmid pRL-TK carrying the Renilla luciferase gene under the control of the simian virus 40 enhancer and promoter (Promega). Lipofectamine 3000 reagent (Invitrogen ...
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bioRxiv - Physiology 2023Quote: ... 300 μl aliquots from each fraction after polysomal ultracentrifugation in sucrose density gradients were spiked with 100 pg of Luciferase mRNA (internal control, Promega). Then ...
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bioRxiv - Molecular Biology 2022Quote: ... Total RNA was extracted from 400 μL of each fraction (spiked with 0.2 ng exogenous control FFLuc mRNA; Promega # L4561) by adding 600 μL TRIzol (Thermo Fisher # 15596018 ...
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bioRxiv - Immunology 2022Quote: ... Cells were immediately stimulated with cytokine for 24 hr and nanoluciferase activity was measured relative to control activity using the NanoGlo Dual Luciferase kit (Promega). The primers for cloning enhancer fragments into pNL3.1 are in Table S3.
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bioRxiv - Immunology 2023Quote: ... pI.18-3xFlag-myRIG-I or pI.18-3xFlag-roRIG-I (25 ng/well each) together with transfection control pLR-SV40-Renilla (Promega) (50 ng/well ...
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bioRxiv - Plant Biology 2023Quote: ... and the control Renilla (REN) luciferase activities were assayed from leaf extracts with the Dual-Glo Luciferase Assay System (Promega) and quantified with a GloMax-Multi Detection System (Promega).
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bioRxiv - Biochemistry 2023Quote: ... Total RNA was extracted from 400 μL of each fraction (spiked with 0.2 ng exogenous control FFLuc mRNA; Promega # L4561) by adding 600 μL TRIzol (Thermo Fisher # 15596018 ...
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bioRxiv - Microbiology 2023Quote: Vero E6 cells were transfected with an GFP expression vector together with either a control expression vector (no spike) or a SARS-CoV-2 spike expression vector using Fugene (Promega). Two hours after transfection ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... was co-transfected at a 1:5 ratio with either the HaloTag-ubiquitin plasmid (expressing Ub-Halo) or the HaloTag control (Promega). Transfected cells were incubated for 20 h at 37 °C ...
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bioRxiv - Bioengineering 2023Quote: ... The fluorescence intensity of each well and empty controls was measured at 520 nm excitation on a Glomax plate reader (Promega). The average blank control values for each plate were subtracted from individual fluorescence intensity values and weekly measurements were normalized to the initial reading.
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bioRxiv - Genomics 2023Quote: DNA was extracted from the agar/cell mixture from the output and control pools using a Wizard Genomic DNA Purification Kit (Promega). The manufacturer’s protocol was followed with two modifications ...
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bioRxiv - Microbiology 2022Quote: ... was cloned by PCR amplification of viral cDNA into a pGEM vector under control of a T7 promoter using pGEM-T Easy Vector System (Promega). RNA standards were subsequently generated by in vitro transcription using the mMESSAGE mMACHINE™ T7 Transcription Kit (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... we transfected 500,000 ANBL6 cells with 1 ug of reporter plasmid plus 250 ng of Rinella plasmids pRL-SV40 and pGL3 control (Promega) in biological triplicates ...
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bioRxiv - Genetics 2023Quote: ... The HALO-NAC61 and GST-HALO (used as negative control) fusion proteins were translated in vitro using the TNTR SP6 coupled reticulocyte lysate system (Promega). Two replicates were used for the TF and input libraries ...
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bioRxiv - Synthetic Biology 2024Quote: ... Cells were transfected the following day with 1,000 ng of either the control plasmid or the BFP-miR-L plasmid using Fugene HD (Promega #E2311) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... Cells were transfected the following day with 1,000 ng of either the control plasmid or the BFP-miRNA plasmid using Fugene HD (Promega #E2311) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: Cells’ viability cultured on control ITO and NM devices was examined with CellTiter-Glo Viability Assay (CTG, Promega, Mannheim, Germany). After sterilization of the devices with 70% ethanol followed by UV irradiation for 30 min ...
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bioRxiv - Molecular Biology 2023Quote: ... we used a pGL3 plasmid with the Firefly luciferase coding sequence followed by a poly-adenylation signal under the control of the SV40 promoter (Promega). The same enhancer sequences we integrated into the reporter locus were amplified by PCR from castaneus mouse strain DNA and inserted downstream of the poly-adenylation signal by Gibson assembly ...
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bioRxiv - Biochemistry 2024Quote: ... the cells were transfected with ISRE reporter plasmid (a gift from Dr. Chia-Wei. Li, Academia Sinica, Taiwan) or with the internal control SV40 renilla plasmid (Promega) for 24 hours and subsequently infected with PR8 at MOI 10 for an additional 16 hours ...
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bioRxiv - Genetics 2024Quote: ... Cells were co-transfected after 24 hours with 50 ng of the CNTNAP5 luciferase reporter constructs along with 10 ng of pRL-TK Renilla luciferase internal control using FuGENE HD transfection reagent (Promega). At 48 hours post-transfection ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: β-arrestin2 recruitment to human chemokine receptors in response to 1 µM VUF15485 or 200 nM positive control chemokines was also monitored by NanoLuc complementation assay (NanoBiT, Promega) (Dixon et al. ...
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bioRxiv - Immunology 2023Quote: ... Secondary antibodies (Promega) were incubated for 1 h at RT ...
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bioRxiv - Molecular Biology 2020Quote: ... then incubated with rabbit or mouse (Promega) HRP-conjugated secondary antibody diluted 1:4000 in 5 % milk powder in TBST for 45 min and washed again ...
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bioRxiv - Cell Biology 2019Quote: ... mouse anti-β-galactosidase (Promega, 1:2000); mouse anti-γ-tubulin (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... 1:1000 TuJ1 (monoclonal mouse, G712A, Promega); 1:250 NeuN (polyclonal chicken ...
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bioRxiv - Microbiology 2021Quote: ... or α-mouse (C1r/s) (Promega, W402B) antibodies conjugated to horseradish peroxidase ...
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bioRxiv - Neuroscience 2019Quote: ... and anti-mouse (1:3000; Promega #W4021) were used for detection with chemiluminescence (HyGLO Chemiluminescent HRP Antibody Detection Reagent ...
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bioRxiv - Cell Biology 2019Quote: ... mouse anti-β-gal (Promega 1:500), chicken-anti-GFP (Invitrogen 1:1000) ...
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bioRxiv - Plant Biology 2020Quote: ... anti-mouse IgG horseradish peroxidase conjugate (Promega). For primary antibody decoration ...