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Citations for Promega :
251 - 300 of 912 citations for Mouse IgG1 Isotype Control Antibody 15H6 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Membranes were washed with TBST and incubated either with anti-mouse or anti-rabbit IgG horseradish peroxidase-conjugated secondary antibody (Promega, 1:10000). Chemiluminescence images were captured by the BioRad ChemiDoc detection system.
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bioRxiv - Cell Biology 2020Quote: ... The PCR product was digested with XhoI and NcoI and ligated into pGL3-control (Promega). The resulting plasmid ...
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bioRxiv - Microbiology 2022Quote: ... The firefly luciferase (FLuc) mRNA was transcribed from the Luciferase T7 Control DNA plasmid (Promega) linearized using XmnI and in vitro transcribed using the mMessage mMachine T7 Kit (Life Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... A Renilla luciferase vector (pGL4.74 [hRluc/TK]) was used as a control (0.001μg/well, Promega) for transfection efficiency ...
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bioRxiv - Cancer Biology 2020Quote: ... The amplified fragment was then cloned into the pGL3-control vector (Promega, Madison, WI, USA) at the NcoI or XbaI sites (restriction enzymes from Takara Bio ...
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bioRxiv - Cell Biology 2022Quote: ... was used for all luciferase reporter assays along with an internal control Renilla plasmid (Promega). Luciferase activity was measured with the GloMax 96-microplate luminometer (Promega ...
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bioRxiv - Molecular Biology 2022Quote: ... The firefly luciferase (FLuc) mRNA was transcribed from the Luciferase T7 Control DNA plasmid (Promega) linearized using XmnI and in vitro transcribed using the mMessage mMachine T7 Kit (Life Technologies ...
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bioRxiv - Genetics 2019Quote: ... We co-transfected five independent luciferase constructs and Renilla control reporter vector (phRL-TK, Promega) using Lipofectamine 2000 (Life Technologies ...
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Structural variant selection for high-altitude adaptation using single-molecule long-read sequencingbioRxiv - Genomics 2021Quote: ... Seg4: 800-1800 bp) were cloned into the pGL3-control plasmid (Promega, Madison, WI, USA), upstream of the SV40 promoter and the firefly luciferase reporter gene ...
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bioRxiv - Evolutionary Biology 2020Quote: ... DNA was extracted individually from whole bees (test; n=300, control; n=165) by Promega Wizard® purification.
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bioRxiv - Genomics 2022Quote: ... The vectors were co-transfected with the pRL-TK Renilla luciferase control reporter vector (Promega) for normalization purposes ...
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bioRxiv - Immunology 2023Quote: ... 1μg of pGL4.10 test plasmid and 0.01μg of pRL-TK control plasmid (Promega Cat. E2241) were added per well ...
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bioRxiv - Microbiology 2023Quote: ... control and CypA mutant HT1080 cells were lysed by adding a passive lysis buffer (Promega) supplemented with protease inhibitor cocktail (Roche) ...
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bioRxiv - Immunology 2024Quote: ... while pGL3-basic and pGL3-promoter-EiH-R1 were negative controls in different experiments (Promega). For each transfection ...
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bioRxiv - Cell Biology 2022Quote: ... blots with α-PRDX6 and α-PfNAPL were incubated with respective α-mouse and α-rabbit horse radish peroxidase (HRP)-coupled secondary antibodies (1:5000, Promega) for 1h at RT ...
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bioRxiv - Molecular Biology 2019Quote: ... incubated with anti-mouse or anti-rabbit HRP-conjugated secondary antibody for 30min (anti-mouse IgG HRP conjugate Promega #W402B, anti-rabbit IgG HRP conjugate Promega #W4011, 1:3000) and chemiluminescence was detected using Western Bright ECL spray (Advansta ...
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bioRxiv - Molecular Biology 2022Quote: ... Then wells were washed again three times with PBS-T (100 μl per well) and incubated with a secondary mouse-HRP antibody (goat, Promega, Madison, WI, USA) (50 μl per well ...
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bioRxiv - Molecular Biology 2022Quote: ... Subsequent to primary antibody removal chitin magnetic beads were washed three times with PBS-T and incubated with secondary mouse-HRP antibody (goat, Promega, Fitchburg, United States) 1:5000 in 100 μl sample buffer on a stirring wheel at room temperature for 1 h ...
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bioRxiv - Molecular Biology 2023Quote: ... the strips were washed in TBS-T for 15 min and incubated with the secondary antibody anti-mouse IgG-HRP (Promega, Cat. no: W4028) at 1:10000 dilution in TBS-T 3 % BSA for 1h ...
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bioRxiv - Cell Biology 2020Quote: ... or mouse (W4021; Promega) IgGs ...
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bioRxiv - Plant Biology 2023Quote: ... or anti-mouse (Promega) in 5% low-fat milk in TBS-T for 1 hour ...
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bioRxiv - Physiology 2019Quote: ... Eluted DNA as well as pre-IP controls were amplified using GoTaq Green Master Mix (Promega) with primers designed for the promoter regions of Ucp1 and Sarcolipin ...
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bioRxiv - Cell Biology 2019Quote: ... These reporter vectors were transfected into HepG2 cells along with a β-galactosidase control plasmid (Promega) to normalize transfection efficiency ...
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bioRxiv - Cancer Biology 2021Quote: ... and pRL-TK vector (as an inner control that contains Renilla luciferase sequences (Promega; Madison, WI)) using Lipofectamine2000 transfection reagent (ThermoFisher ...
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bioRxiv - Biochemistry 2020Quote: ... Two control reactions were performed: one containing 8 units of HeLa nuclear extract provided by Promega as a positive control and the other containing no nuclear protein as a negative control ...
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bioRxiv - Plant Biology 2019Quote: ... we used a control plasmid provided in the TnT® Quick Coupled Transcription/Translation System (Promega). The plasmid was linearized using the restriction enzyme SacI and purified using the DNA Clean and Concentrator Kit (Zymo Research) ...
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bioRxiv - Molecular Biology 2024Quote: ... About 1.2 μg WT or constructed pGL4.24 plasmid and 0.2 μg pGL4.74 control plasmid (Promega, #E6921) were co-transfected into K562 cells by Neon™ NxT Electroporation System (Invitrogen) ...
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bioRxiv - Immunology 2023Quote: ... equal amounts (∼500 µg) of each sample were treated with RNaseIN as control (Promega, 40 U), or an RNase cocktail (per sample ...
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bioRxiv - Neuroscience 2023Quote: ... Mutations additionally identified with an AAF suggestive of a germline event in control DNA samples (Promega genomic control and unrelated individual’s brain tissue) ...
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bioRxiv - Neuroscience 2023Quote: ... The cDNA library was subjected to quality control by a QuantiFluor dsDNA system (E2671, Promega, WI) and an Agilent D5000 ScreenTape system before being sent to scRNA-seq (NovaSeq 6000 ...
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bioRxiv - Immunology 2024Quote: ... Quality control for library quantity and size distribution was done using the QuantiFluor dsDNA System (Promega) and High Sensitivity NGS Fragment Analysis Kit (Agilent Technologies) ...
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bioRxiv - Molecular Biology 2024Quote: ... Extracts were validated for transcription using the positive control reagents provided in the commercial HeLaScribe (Promega) kit and subsequent in vitro transcription assays with HepG2 extract were performed as described above for the commercial HeLa extract ...
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bioRxiv - Neuroscience 2021Quote: ... Either pCAGIG-Emx2 or pCAGIG were cotransfected with pNfib or the control pGL4.23 luciferase reporter plasmid (Promega) using FuGENE HD (Promega) ...
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bioRxiv - Molecular Biology 2019Quote: PUER cells were transfected with Cebpa promoter reporter vector and Renilla control vector pRL-CMV (Promega, E2261) in a 1:200 ratio using a 4D-Nucleofector (Lonza) ...
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bioRxiv - Cancer Biology 2019Quote: ... The luciferase gene used for the control was cloned from the pGL3-Basic Vector (Promega, Madison, WI). All inserts were confirmed by DNA sequencing ...
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bioRxiv - Genomics 2019Quote: ... of the relevant pCpGL-EF1 plasmid and 6ng (DNA methylation) or 1.5ng (SOX9 overexpression) of pRL-TK Renilla control plasmid and 0.3μl FuGeneHD reagent (Promega) per well ...
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bioRxiv - Cancer Biology 2019Quote: ... The luminescence values were transfection normalized with the internal control pRL-TK (50 ng, Renilla Luciferase; Promega). Experiments were performed with three biological replicates.
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bioRxiv - Immunology 2021Quote: Transfection was done in BEGM media in 96-well dishes (65-85% confluent) with 0.098 ug CLEC7A.luc and 0.002 ug pGL4.PGK control vector per well (Promega) using Trans-IT X2 (Mirus) ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA was isolated from control or DDX39B-depleted cells using ReliaPrep RNA Cell Miniprep System (Promega) or Direct-zol RNA kit (Zymo Research) ...
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bioRxiv - Molecular Biology 2019Quote: ... Instrument quality control was monitored using the Promega 6×5 LC-MS/MS Peptide Reference Mix (Promega) before and after each MS experiment run ...
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bioRxiv - Microbiology 2024Quote: ... 20 µg of the plasmid was co-transfected with 2.5µg of pGL4.75 Renilla luciferase control vector (Promega) into DT40 AIDR/puro UNG-/- cells ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Samples serving as positive controls for the cytotoxicity measurement received 72 μg/mL membrane-permeabilizer digitonin (Promega) in respective ToxGlo Media type (glucose or galactose) ...
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bioRxiv - Genetics 2023Quote: ... with 500 ng reporter and 50 ng pRL Renilla Luciferase Control Reporter vector (Promega Cat. No. E2231). Luciferase intensity was measured 24 hours after transfection with Dual-Luciferase® Reporter Assay System (Promega ...
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bioRxiv - Cell Biology 2023Quote: ... Genotyping to identify bPAC and Drer_Chr1 (DNA extraction control) were performed using GoTaq Green master mix (Promega). DNA samples for genotyping were extracted (lysis buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... AntiHaloTag (mouse monoclonal, Promega G9211) blot (Figure 1B ...
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bioRxiv - Cell Biology 2023Quote: ... or anti-mouse HRP (Promega) was diluted in TBST containing 5% dried nonfat milk ...
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bioRxiv - Molecular Biology 2023Quote: ... mouse anti-HaloTag (Promega, G921A), rabbit anti-DDX6 (Bethyl ...
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bioRxiv - Cell Biology 2023Quote: ... mouse anti-HaloTag (Promega, G9211); rabbit anti-CIMPR (made in house) ...
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bioRxiv - Cell Biology 2023Quote: ... Halo-Tag (Promega, G9211, mouse), and HSP90 (Santa Cruz Biotechnologies ...
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bioRxiv - Physiology 2024Quote: ... or anti-mouse HRP (Promega) was diluted in TBST containing 5% dried nonfat milk ...