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Citations for Promega :
3901 - 3950 of 4270 citations for 7 Bromo 1 2 3 4 tetrahydronaphthalen 1 ol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... the cells were transfected with 1 μg of DNA (1 μg of an L1-expressing plasmid or 0.5 μg of the L1-expressing plasmid and 0.5 μg of either a pCMV-3Tag-8-Barr control or ISG-expressing plasmid) using 3 µL of FuGENE HD transfection reagent (Promega, Madison, WI, United States) and 100 µL of Opti-MEM (Gibco ...
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bioRxiv - Neuroscience 2022Quote: ... the pHAD plasmid was linearized by PmeI enzyme to expose the ends of the 5’ and 3’ inverted terminal repeats (ITRs) and transfected into 116 producer cells (Profection® Mammalian Transfection System, Promega, Madison, WI, USA). Helper virus (HV ...
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ADEVO: Proof-of-concept of Adenovirus Directed EVOlution by random peptide display on the fiber knobbioRxiv - Bioengineering 2023Quote: ... Media was changed at 3 hpi and luciferase luminescence was measured at 24 hpi using the Nano-Glo® Luciferase Assay (Promega, Madison, USA, #N1130) kit ...
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bioRxiv - Biochemistry 2019Quote: ... N-glycans were released from the membrane-bound protein using 2 U PNGase F (Promega, WI, USA) with overnight incubation at 37°C ...
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bioRxiv - Genetics 2020Quote: ... 2 µg of DNA in 50 µl of DMEM and 50 µl of FuGene6 transfection reagent (Promega) at 1mg·ml-1 in DMEM were incubated separately for 5 min at room temperature ...
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bioRxiv - Bioengineering 2021Quote: ... The results were calculated using the 2-△△CT method according to the GoTaq qPCR Master Mix (Promega) manufacturer’s specifications.
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bioRxiv - Plant Biology 2020Quote: ... genomic DNA was extracted from 2-wk old seedlings using the Wizard Genomic DNA purification kit (Promega) and was subjected to PCR analysis using two AtNIP2;1 gene specific primers and the left border T-DNA primer (all primers used in this study are described in Supplemental Table 1) ...
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bioRxiv - Plant Biology 2021Quote: ... Selection was done twice for 2 weeks on medium containing 25 µg/ml G418 (Promega, Mannheim, Germany) starting 2 weeks after transformation with a 2-weeks release period in between ...
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bioRxiv - Developmental Biology 2022Quote: ... The results were calculated using the 2-ΔΔCT method according to the GoTaq qPCR Master Mix (Promega) manufacturer’s specifications.
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The plasmid for β-arrestin-2 fused at the N-terminus to LgBiT was obtained from Promega custom assay services (plasmid CS1603B118) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... or with 0.05 µg GLP-1R-SmBiT and 0.05 µg LgBit-β-arrestin-2 (Promega, Hampshire, UK) plus 0.9 µg pcDNA3.1 for 24 hours ...
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bioRxiv - Molecular Biology 2019Quote: The entire genome was amplified by PCR using GoTaq 2× Hot Start Colorless Master Mix (Promega, USA) using overlapping primer sets (Ali et al. ...
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bioRxiv - Cell Biology 2020Quote: KNL-2-MBP fragments were [35]S labeled using TnT® Quick Coupled Transcription/Translation System (Promega). 10 μl of the in vitro translation lysate was incubated for 1hr at 4°C with 50 μg CENP-AHCP-3(1-109)-MBP-His (in 20 mM Tris [pH7.5] ...
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bioRxiv - Biophysics 2021Quote: ... Constructs (2 μg) were transfected into HEK293 cells on glass coverslips using Fugene HD (Promega, Madison, WI) per manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... The results were calculated using the 2-△△CT method according to the GoTaq qPCR Master Mix (Promega) manufacturer’s specifications.
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bioRxiv - Cancer Biology 2021Quote: ... cDNA was synthesised from 2 μg RNA using the GoScriptTM Reverse Transcription kit (Promega, UK, Cat. A5003). Primers were designed using NCBI BLAST primer design or ...
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bioRxiv - Cell Biology 2022Quote: ... P2-2: -212- -826) were cloned through PCR into the pGL4.20 luciferase reporter vector (Promega, Madison, WI) using C57BL/6 genomic DNA as a template ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were in-gel digested by addition of 200 µL of 2 ng/µl trypsin (Promega, V511A) in resuspension buffer (Promega ...
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bioRxiv - Microbiology 2022Quote: The intron 2 region was amplified from genomic DNA with GoTaq G2 Flexi DNA polymerase (Promega, M7801) in a final volume of 20 μl as follows ...
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bioRxiv - Molecular Biology 2022Quote: Cell proliferation was measured in BAG-1 CRISPR knockout cells or siRNA treated cell in response to vehicle (0.1% DMSO) and Thio-2 (5μM and 50μM) using CellTiter-Glo (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... 2-4μg bacmid was transfected into Sf9 cells in 6 well plates using Insect-XPRESS (Promega E2311). Cells were incubated at 2°C for 5 days and bacmid YFP expression assessed by Leica DM IL LED Fluo microscope using a green fluorescent protein filter cube ...
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bioRxiv - Microbiology 2023Quote: ... 2 mM TCEP) was added to the sample followed by 40 µg of sequencing grade trypsin (Promega). Proteins were digested overnight rotating at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... measured using a Biotek Synergy 2 multimode plate reader and the BacTitr-Glo Cell Viability assay (Promega). There was a linear relationship between concentration and luminescence (R2 =0.998) ...
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bioRxiv - Immunology 2023Quote: ... Tryptic digest was performed in 50 mM ammonium bicarbonate buffer with 2 μg sequencing-grade trypsin (Promega) at 37°C overnight ...
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bioRxiv - Cell Biology 2022Quote: ... cells were transfected with 2 ug of pQCXIN ERAAP-dsRed using Fugene HD transfection reagent (Promega, E2311) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... and 2 μg of the linearized DNA was transcribed in vitro using T7 RNA polymerase (Promega Corporation). The obtained RNA was then purified using GeneJET kit (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were incubated with 20 μl of 4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide (MTT) (AqueousOne, Promega) at 37°C for 1-2 hours for MTS colorimetric cell viability assay ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 0.5 µg (HEK293A, HEK293T, HEK293AΔARRB1/B2) or 2 µg (HEK293AΔGs, HEKS293AΔGi) Glo-22F cAMP plasmid DNA (Promega) and 2 µg MOCK DNA (HEK293A ...
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bioRxiv - Biochemistry 2024Quote: ... Each sample was divided into 2 aliquots and digested with trypsin (Trypsin Gold, Mass Spectrometry Grade, Promega) and AspN (Sequencing Grade ...
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bioRxiv - Cancer Biology 2024Quote: ... Urea was diluted to working 2 M concentration with 20 mM HEPES (pH 8.0) and tryptic (Promega) digestion performed at an enzyme to substrate ratio of 1:25 overnight with incubation at 37°C ...
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bioRxiv - Biochemistry 2024Quote: ... The reactions were incubated for 2 h at 37 °C followed by DNase RQ1 (Promega, Madison, WI) treatment (2 Units ...
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bioRxiv - Microbiology 2024Quote: ... ATP content was measured using the BacTiter-Glo™ Microbial Cell Viability Assay Kit (Promega, Cat.G8230/2). Briefly ...
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bioRxiv - Cell Biology 2021Quote: ... 30 min at 60°C) an alkylation (IAA 0.5M, 30 min RT) microtubule-associated protein enriched fractions were digested using trypsin (Gold, Promega, 1 μg / sample, overnight at 30°C). Peptide clean-up was done using OMIX C18 (Agilent ...
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bioRxiv - Microbiology 2020Quote: ... alkylation was performed using 20 mM 2-chloroacetamide at room temperature in HEPES buffer for 30 min under exclusion of light. Samples were prepared according to the SP3 protocol (Hughes et al. 2019) and trypsinized (sequencing grade, Promega, enzyme to protein ratio 1:50) overnight at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Each organ was homogenized with a lysis solution containing a 1:5 dilution of Luciferase Cell Culture Lysis 5X Reagent (Promega, Madison, WI, USA, cat. no. E1531) in Millipore water supplemented with 1 % (v/v ...
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bioRxiv - Microbiology 2019Quote: ... cells were pelleted at 1000 rcf for 5 min at 4 °C and resuspended in luciferase reporter lysis buffer (Promega, Cat. #E4530) with 1% protease inhibitor cocktail (Millipore Sigma ...
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bioRxiv - Genomics 2022Quote: The 293T cells in 96-well plates were transiently transfected with 200 ng Firefly luciferase vector (pGL3) and 4 ng pRL-TK Renilla luciferase vector (Promega, Madison, USA) using 0.5 uL Lipofectamine 3000 (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2019Quote: ... fixed with 4% paraformaldehyde and labeled with rabbit polyclonal anti-GFP (Minotech Biotechnology) and mouse anti-β-galactosidase (Promega Cat# Z3781, RRID:AB_430877) primary antibodies ...
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bioRxiv - Pathology 2021Quote: ... 30 μg of protein was digested with Lys-C (FUJIFILM Wako Chemicals Europe GmbH, Germany) for 4 h and subsequently with modified porcine trypsin (Promega, WI, USA) for 16 h at 37 °C.
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bioRxiv - Plant Biology 2020Quote: ... The columns were capped at the bottom and 200 µl AmBic containing 4 ng/µl of Trypsin + LysC (Promega Catalog number V5073) was added to each sample and incubated in a 37°C shaker for 16 hours ...
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bioRxiv - Molecular Biology 2022Quote: ... Pelleted cells were disrupted by glass beads agitation at 4°C in 1x Passive Lysis Buffer provided in the Dual-Luciferase® Reporter Assay System (Promega, #E1910). Extracts were clarified by centrifugation ...
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bioRxiv - Molecular Biology 2024Quote: ... The clear supernatant was incubated overnight at 4°C with 100 μl of prewashed Magne® HaloTag® Beads (Promega, WI, USA). Post-incubation ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated for 24-48hrs in the blocking solution containing chicken anti-human NT-4/5 antibody (10 mg/ ml, Promega, Madison, WI) or rabbit anti-human NT-4/5 (1:1000 ...
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bioRxiv - Genomics 2023Quote: ... Primers bearing kpnI and BgLII sites (Additional Table 4) allowed incorporation into pGL3-Basic reporter vector containing luciferase gene from the firefly Photinus pyralis (Promega, Wisconsin, USA). Amplification was carried out using Phusion HotStart II Polymerase ...
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bioRxiv - Immunology 2023Quote: ... 4 µl of cDNA per sample were used and qRT-PCR was performed using the GoTaq® qPCR Master Mix (Promega, #A6001) on a LightCycler 96 (Roche) ...
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bioRxiv - Cancer Biology 2023Quote: ... Luminescence was measured 24 h after plating (T0) and after 4 d using the CellTiter-Glo Luminescent Cell Viability Assay protocol (Promega; cat# G7573). When indicated ...
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bioRxiv - Microbiology 2024Quote: ... Cells infected with viruses expressing luciferase were harvested 4 days after infection and analyzed for luciferase activity with a luminescence kit (Bright-Glo™ Luciferase Assay System, Promega, E2620) as described in (61).
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bioRxiv - Microbiology 2020Quote: ... for 3 hours before being diluted with 5 volumes of 40 mM NH4HCO3 and digested overnight with sequencing grade modified trypsin (Promega, Madison, WI, USA; 1/50 w/w). Digested samples were acidified to a final concentration of 0.5% formic acid and desalted with 50 mg tC18 Sep-Pak columns (Waters corporation ...
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bioRxiv - Cell Biology 2019Quote: ... Cell signalling) or rabbit anti-TgTOM40 (1:2000, (van Dooren et al., 2016)) antibodies coupled to secondary horseradish peroxidase (HRP)(Promega for mouse and rabbit, Abcam for rat) conjugated antibodies (1:10,000 ...
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bioRxiv - Biochemistry 2021Quote: ... The dried resin was resuspended with 50 mM Tris pH 7.5 (2x resin volume) and 200 ng of trypsin was added (Promega; made up to 100 ng·μL-1 in H2O), the samples were digested overnight shaking at 37°C ...