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Citations for Promega :
301 - 350 of 620 citations for L lactate dehydrogenase B chain LDH B Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... 8 mL/L glycerol (Promega) and 10 g/L NaCl and shaken at 200 RPM overnight at 37°C ...
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bioRxiv - Microbiology 2019Quote: ... Quantitative real-time polymerase chain reaction (PCR) was performed for CGRP with GoTaq® qPCR Master Mix (Promega) using the DNA-binding dye ...
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bioRxiv - Cancer Biology 2023Quote: ... HEK cells were transfected with both heavy and light chain BCR constructs using FuGENE HD transfection reagent (Promega) in a 1:3 ratio ...
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bioRxiv - Cancer Biology 2019Quote: ... cDNA was stored at −20°C until analysed by polymerase chain reaction using GoTaq®G2 DNA polymerase (Promega) according to the manufacturers protocol ...
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bioRxiv - Immunology 2021Quote: ... Confirmed heavy and light chain plasmid DNA were co-transfected in 293T cells (ATCC) using Fugene transfection reagent (Promega) in 24 well plates for preparing antibody supernatant for initial screening for their expression and antigen specificity as detailed in the following section ...
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bioRxiv - Immunology 2023Quote: ... 1 mmol/L dithiothreitol (V315A, Promega), 5 mmol/L β-glycerophosphate (G6376 ...
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bioRxiv - Systems Biology 2020Quote: ... Human cell lysate (MS Compatible Human Protein Extract, Digest, V6951) were purchased from Promega.
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bioRxiv - Neuroscience 2020Quote: ... All designed probes were tested by ddPCR using human genomic DNA (Human male, Promega) as a negative control ...
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bioRxiv - Molecular Biology 2022Quote: ... Human Genomic DNA (G1521, Promega) was used as positive control and synthetic DNA was used to validate the LAMP assay performance ...
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bioRxiv - Molecular Biology 2020Quote: ... and real-time polymerase chain reaction (RT-PCR) conducted using the Techne Prime Pro thermal cycler with GoTaq qPCR Master Mix (Promega). RT-qPCR reactions were carried out in 10 μL volumes containing 3.5 mM MgCl2 ...
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bioRxiv - Microbiology 2022Quote: All isolates were heat-extracted at 95°C for 20 minutes for polymerase chain reaction (PCR) using GoTaq (Promega M7132) and 8F and 1492R primers to amplify the whole 16S rRNA gene (4) ...
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bioRxiv - Neuroscience 2019Quote: ... The quality of the cDNA was tested using GPDH primers (see below for sequence) and GoTaq endpoint polymerase chain reaction (PCR) protocol (Promega). Quantitative PCR was performed using a SYBR Green protocol (Applied Biosystems ...
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bioRxiv - Zoology 2021Quote: ... Polymerase chain reactions in a 15 μL solution containing 7.5 μL GoTaq® G2 Hot Start Green Master Mix (Promega), 0.4 μmol of each primer and 46 – 247 ng of genomic DNA were performed using a C1000 Touch Thermal Cylinder (BioRad) ...
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bioRxiv - Evolutionary Biology 2020Quote: To generate the pGl3-STK38-P luciferase reporter,1.2 kb of DNA upstream the STK38 TTS promoter region with ZNF611 binding motif was amplified by polymerase chain reaction (PCR) and cloned into the Firefly luciferase reporter plasmid pGL3-Basic (Promega) using KPN1 and XHO1 restriction sites.0.7 kb of DNA upstream the STK38 TTS promoter region without ZNF611 binding motif was cloned into pGL3-Basic using same restriction sites to generate the pGl3-STK38-ΔP luciferase reporter ...
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bioRxiv - Molecular Biology 2023Quote: ... was removed by evaporation using speed-vac and the residue was used to produce L-[2,3,4,5-3H]-proline labeled procollagen chains and the collagen-like proteins in the TnT® Coupled Reticulocyte Lysate Systems (Promega) using either SP6 or T7 promoters ...
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bioRxiv - Neuroscience 2022Quote: ... 10µl RNasin RNase-inhibitor (40U/l, Promega) were added ...
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bioRxiv - Immunology 2023Quote: ... HRP-anti-Mouse IgG (H+L) (Promega).
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bioRxiv - Cancer Biology 2023Quote: The assessment of gene expression was performed by quantitative real-time Polymerase Chain Reaction (qPCR) using the GoTaq® qPCR Mix (Promega) in the 7500 Fast Real Timer PCR system (Applied Biosystems®) ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were stably transduced with a lentivirus-based expression construct generated by cloning a polymerase chain reaction (PCR)-amplified DNA fragment encoding luciferase from a pGL4.10-lucIIplasmid (Promega, Sydney, Australia) into a pLenti CMV Hygro DEST vector (Addgene ...
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bioRxiv - Microbiology 2023Quote: ... Marker genes in isolated DNA were polymerase chain reaction (PCR)-amplified using GoTaq Master Mix (Cat. No. M5133, Promega, Madison, Wisconsin) and 515 F (5′-GTGCCAGCMGCCGCGGTAA-3′) ...
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bioRxiv - Microbiology 2020Quote: ... 500 ng human genomic DNA (Promega) or 5 μl of untreated or RNase-treated nasal swab nucleic acid isolates ...
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bioRxiv - Cancer Biology 2020Quote: ... Pooled human male genomic DNA (Promega) was randomly sheared (500-800bp ...
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bioRxiv - Systems Biology 2021Quote: ... a human cDNA pool (Promega Corporation), or obtained as synthesized double-stranded DNA fragments (gBlocks ...
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bioRxiv - Genomics 2021Quote: ... 50ng human genomic DNA (Promega, G304A), 2µM assembled T7-Tn5 transposase or Tn5 transposase was incubated at 55 °C for 7minutes ...
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bioRxiv - Immunology 2023Quote: ... Lumit Human IL-1β Immunoassay (Promega) was performed according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... human Female genomic DNA (Promega #G1521), as a reference ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... CP4 medium was exchanged with Hanks’Balanced Salt Solution (with added glucose 1 g/l, NaHCO3 0.35 g/l) containing GloSensor cAMP Reagent (3 %, Promega) and plates were incubated for 60 min ...
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bioRxiv - Molecular Biology 2022Quote: ... supernatant was collected and purification was done via His-tag using HisLink Protein Purification Resin (V8823, Promega) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: Cell-free translated His-VqmA was produced using the S30 T7 High-Yield Protein Expression System (Promega) according to the manufacturer’s protocol with the following amendments ...
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bioRxiv - Plant Biology 2024Quote: ... The soluble GST and His fusion proteins were purified using the MagneGST Protein Purification System (Promega, USA) or the MagneHis Protein Purification System (Promega ...
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bioRxiv - Immunology 2019Quote: ... from C57BL/6J or MD4 mice that had been pre-treated with CK-689,CK-666 were added to COS-7 APCs expressing either the single-chain anti-Ig,𝜅 surrogate Ag or mHEL-HaloTag that was labeled with HaloTag tetramethylrhodamine ligand (Promega, #G8251). After the indicated times at 37°C ...
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bioRxiv - Microbiology 2019Quote: ... Polymerase Chain Reaction (PCR) targeting recA was performed in 50 µl volumes using 10 µl of 5X buffer (Promega, Madison, WI, USA), 7 µl of 25 mM MgCl2 (Promega ...
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bioRxiv - Microbiology 2021Quote: ... Anti-rabbit IgG (H+L) HRP Conjugate (Promega) was used as the secondary antibody ...
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bioRxiv - Cell Biology 2021Quote: ... and Anti-Rabbit IgG (H+L) Conjugate (Promega).
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bioRxiv - Cell Biology 2021Quote: ... 1.25 ng/L porcine trypsin (Promega, Cat No:V5280) in 25 mM NH4HCO3 was added to the gel pieces ...
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bioRxiv - Cell Biology 2020Quote: ... 10 mmol/L EDTA (Cat. No. V4231 Promega), and 100 mmol/L NaCl (Cat ...
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bioRxiv - Biophysics 2021Quote: ... The 2725-nt RNA molecules were synthesized by polymerase chain reaction (PCR) amplification of the plasmids followed by in vitro transcription using the T7 RNA polymerase (Promega, Fitchburg, WI, USA). The RNAs contain a 1206-nt upstream sequence ...
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bioRxiv - Biochemistry 2020Quote: ... This strain (BL21DE3/pETDuet-1-6xhis-TEV-ftsE) was grown until OD600 of 0.5 and his-FtsE expression was induced with 0.3 mM IPTG (Promega) during 3 hours at 28°C ...
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bioRxiv - Plant Biology 2022Quote: ... His-tagged and GST-tagged recombinant proteins were purified using Promega MagneHis™ Ni-Particles (Promega Cat#V854A) and MagneGST™ Glutathione Particles (Promega Cat# V861A ...
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bioRxiv - Cell Biology 2020Quote: ... The complete Igκ-LaNt α31-Myc-His sequence was inserted into pGEM®-5Zf(+) vector (Promega, Madison, WI) using NheI and PmeI (New England Biolabs) ...
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bioRxiv - Microbiology 2019Quote: ... Human genomic DNA (Promega, Madison, WI, USA) was diluted to 10,000 genome copies per μl then serially diluted to 10 genome copies per μl and used to create a human DNA copy number standard curve ...
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bioRxiv - Molecular Biology 2019Quote: ... The natural templates include purified human (Promega), rhesus monkey (BioChain) ...
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bioRxiv - Molecular Biology 2019Quote: ... 10.0 ng/ml recombinant human FGF (Promega) and 1.0 μM dexamethasone (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2020Quote: ... Human Genomic DNA Male (Promega, cat # G1471) and IDH1 R132H Reference Standard (Horizon ...
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bioRxiv - Cancer Biology 2020Quote: ... 50ng of normal human genomic DNA (Promega) was used as a negative control ...
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bioRxiv - Cancer Biology 2021Quote: ... Human LAMA1 cDNA was purchased from Promega and sub-cloned into pLVX-Tet-On Advanced vector (Clontech) ...
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bioRxiv - Genomics 2021Quote: Commercial human DNA (Promega, Cat. No. G1521) was used for PCR amplification of all tested genome fragments from AF associated loci (for primers used see Supplementary Table S1 ...
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bioRxiv - Microbiology 2023Quote: ... Human gDNA (Promega, San Luis Obispo, CA) was added to the extracted gDNA from the clinical swabs to reach a total input of 3 μg/130uL for fragmentation and library prep ...
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bioRxiv - Immunology 2022Quote: ... These included 5μg/ml human gDNA (Promega), 500ng/ml citrullinated histones (Cayman Chemical #501620) ...
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bioRxiv - Genomics 2023Quote: ... 1 ug human DNA (Promega, Madison, WI), 5 ug sheared salmon sperm DNA (Promega ...