Labshake search
Citations for Promega :
451 - 500 of 620 citations for L lactate dehydrogenase B chain LDH B Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2020Quote: The region of interest (750-850 bp) was PCR amplified from pooled male human DNA (Promega) and cloned into a STARRseq luciferase validation vector_ORI_empty plasmid (Addgene plasmid #99298 ...
-
bioRxiv - Molecular Biology 2020Quote: ... WT or mutant promoter of human Rpl28 gene was cloned into pGL-3-basic (Promega, E1751). The number is relative to transcriptional start site ...
-
bioRxiv - Bioengineering 2020Quote: ... and detected with anti-rabbit or anti-human HRP-conjugated secondary antibodies (Promega W4011 and W4031). Blots were developed using ECL (ThermoScientific 32106 ...
-
bioRxiv - Cancer Biology 2023Quote: ... Human and murine NLRC5 and CXCL10 promoters were cloned into pGL3 basic luciferase reporter vector (Promega). All mutated plasmids were constructed from wild type plasmids by Fast Mutagenesis System (TransGen Biotech) ...
-
bioRxiv - Cell Biology 2023Quote: ... 10mM Ultrapure ATP and 0.7 μg of recombinant full-length human CDC7/DBF4 kinase (Promega, V5088). Reactions were incubated at 37 °C for 120 min and then terminated by freezing on dry ice before mass spectrometry.
-
bioRxiv - Immunology 2024Quote: ... 3×106 ADCC and ADCP Bioassay Effector Cells expressing the human FcγRIIIa or FcγRIIa receptors (Promega) were added per well and the cells were incubated in at 37°C with 5% CO2 for 6 hours ...
-
bioRxiv - Molecular Biology 2022Quote: ... The urea concentration was then diluted with 17 μl of 50 mM HEPES pH 8.5 and after adding 1 μl of 0.2 mg/ml trypsin (Promega) in 0.2 mM HCl the digestion continued overnight at 37°C with interval mixing at 2000 RPM for 30 s every 15 minutes ...
-
bioRxiv - Cell Biology 2021Quote: ... Membranes were washed with PBST and then anti-mouse IgG (H&L) alkaline phosphatase-conjugated secondary antibody (Promega) was incubated with the membrane at a 1:5,000 dilution for 1 h at RT ...
-
bioRxiv - Neuroscience 2020Quote: 80,000 HEK293T cells were plated in 500μl HEK Media and 24 hrs later 500ng total plasmid DNA was transfected with 1.5μl FuGene HD (Promega) as follows ...
-
bioRxiv - Biophysics 2022Quote: ... The poly-L-lysine coated side of the cover slip was exposed to 5 mg/ml streptavidin (Promega) in this space for 15 min ...
-
bioRxiv - Microbiology 2022Quote: ... mutations were generated in a fragment of the L ORF subcloned into a pGEM T easy vector (Promega). The mutated ORF fragment was sequenced and then substituted in the L ORF of pTM1 ...
-
bioRxiv - Cancer Biology 2022Quote: ... 20,000 to 50,000 cells were lysed in the transposase reaction mix (12.5 μl 2xTD buffer, 2 μl TDE1 [Illumina], 10.25 μl nuclease-free water, and 0.25 μl 1% digitonin [Promega]) for 30 min at 37 °C ...
-
bioRxiv - Molecular Biology 2024Quote: ... The samples were diluted with 17 µL of 50 mM HEPES (pH 8.5) and digested with 1 µL of 0.2 mg/mL trypsin (Promega) in 0.2 mM HCl at 37°C with interval mixing at 2000 RPM for 30 sec every 15 min.
-
bioRxiv - Cancer Biology 2021Quote: Each enhancer or promoter region was amplified from human genomic DNA and cloned into pGL4.10 [luc2] (Promega) containing a SNP (rs718960 ...
-
bioRxiv - Neuroscience 2020Quote: ... and pCMVΔ8.9 plasmids were co-introduced into human embryonic kidney 293T cells using the FuGENE reagent (Promega). 48 h after transfection ...
-
bioRxiv - Microbiology 2020Quote: ... albicans to damage human vascular endothelial cells was assessed by the CytoTox-96 assay (Promega, Madison, WI), which measures the release of lactate dehydrogenase (LDH ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... cells were transfected with human RXFP1 pcDNA-Zeo-tetO and the GloSensor reporter plasmid using FuGENE (Promega), according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2021Quote: ... HEK 293T cells were co-transfected with human MOR and a luciferase-based cAMP biosensor (GloSensorTM, Promega). The next day ...
-
bioRxiv - Genetics 2022Quote: We performed transient transfection on human pluripotent stem cells using FuGENE® HD Transfection Reagent (Promega, E2311). The cells were splitted and treated with Y-27632 for one before transfection.
-
bioRxiv - Genomics 2023Quote: ... Primer efficiency was calculated using a 10-fold serial dilution of Human Mixed Genomic DNA (Promega G3041). For primer sequences and calculated efficiencies ...
-
bioRxiv - Cell Biology 2024Quote: All human cell lines were authenticated at each batch freezing by STR profiling (StemElite ID System, Promega). All cell lines were tested for mycoplasma at each batch freezing by PCR (32 ...
-
bioRxiv - Biochemistry 2019Quote: ... 70 000-100 000 cells (in 200-450 μl DMEM) were seeded into each well containing a transfection cocktail of 0.4 μl of Fugene 6 (E2693, Promega), with 20 μl Opti-MEM (11058-021 ...
-
bioRxiv - Cell Biology 2019Quote: ... were transfected with 1 µg of DNA and 4 µL FuGENE 6 in 100 µL Opti-MEM media for 24-36 hours according to the manufacturer’s instructions (Promega). Cells were fixed with 4% (v/v ...
-
bioRxiv - Plant Biology 2020Quote: ... 10 mg/L BSA and 1 mM PMSF) and assayed using the Dual-Luciferase® Reporter Assay System (Promega). Experiments were normalized to negative control set at 1.
-
bioRxiv - Pathology 2021Quote: ... and 20 µg/mL L-012) and recorded by a Promega GloMax Navigator microplate luminometer (Promega Biotech, Beijing, China). GraphPad Prism software was used to analyze data.
-
bioRxiv - Molecular Biology 2022Quote: ... and purified on a 10g/L agarose gel using the Wizard SV Gel and PCR Clean-Up system (Promega). All mixtures were made in 1x NEBuffer 3.1 on ice with final concentrations of 80nM Cas9 ...
-
bioRxiv - Plant Biology 2022Quote: ... Luciferase activity of cell extracts was measured with Sirius L Tube Luminometer (Berthold) and the luciferase assay system (Promega).
-
bioRxiv - Genomics 2021Quote: The supernatant was magnetically removed and the beads were resuspended in 20 µl of L3 DNA linker ligation mixture (8 µl water, 5 µl 4X ligation buffer, 1 µl RNA ligase [New England Biolabs], 0.5 µl RNasin [N2615, Promega GmbH] ...
-
bioRxiv - Cell Biology 2021Quote: ... the supernatant was removed and the nuclei pellet was resuspended into 500 µl nuclei storage buffer (NSB: 1x PBS; Invitrogen, 0.5% RNase free BSA;Serva, 1:200 RNaseIN plus inhibitor; Promega, 1x EDTA-free protease inhibitor ...
-
bioRxiv - Cell Biology 2022Quote: ... were seeded onto 6-well plates coated with 0.1 mg ml−1 poly-L-lysine and transfected using FuGENE HD transfection reagent (Promega) with a mixture of third-generation lentiviral backbone vector pJHUMCS that encodes BDNF-pHluorin ...
-
bioRxiv - Biochemistry 2023Quote: ... The sample was then diluted with 120 μL of PBS before 1.2 μL of ProteaseMax surfactant (0.1 mg/mL in 100 mM ammonium bicarbonate, Promega, V2071) and sequencing grade trypsin (10 μL ...
-
bioRxiv - Neuroscience 2023Quote: ... Then the beads were incubated with 80 μL of 2 M urea in 50 mM Tris-HCL containing 1 mM DTT and 0.4 μg trypsin (Promega) at 25 °C for 1 hour while shaking at 1,000 rpm ...
-
bioRxiv - Microbiology 2023Quote: ... 20 μL of MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) inner salt (Promega) was added to each well and the plate was further incubated for 1 h at 37 °C ...
-
bioRxiv - Molecular Biology 2021Quote: ... Cells were transfected with a control Flag or HA-GSK-3βWT human plasmid using Fugene 6 (Promega #E2693) in a 2:1 ratio and incubated for 3 hours in a CO2 incubator ...
-
bioRxiv - Cancer Biology 2020Quote: ... T47D cells and MCF7 cells were transfected with empty or human LIP-containing pcDNA3.1 via Fugene HD (Promega) using the manufactures protocol ...
-
bioRxiv - Cancer Biology 2021Quote: The upstream 376 bp region of the human LGALS1 transcriptional start site was cloned into the pGL4.23 (Promega) vector to generate the LGALS1 luciferase reporter gene (LGALS1 pGL4.23 ...
-
bioRxiv - Cancer Biology 2022Quote: ... and MV4-11 (DSMZ Cat# ACC-102, RRID:CVCL_0064) human AML cell lines were identified by PCR-single-locus-technology (Promega, PowerPlex21 PCR Kit ...
-
bioRxiv - Biophysics 2019Quote: The cell-viability of human neuroblastoma (SH-SY5Y) cells was measured using MTT cell proliferation assay (Promega, G4000). SH-SY5Y cells were plated in a 96-well plate followed by differentiation in Neurobasal-A ...
-
bioRxiv - Molecular Biology 2019Quote: Full length 3’-UTR of human (P)RR and LDLR were cloned into the luciferase reporter vector (Promega). Mutant 3’-UTR luciferase reporter vectors were generated by PCR using site-directed mutagenesis technique ...
-
bioRxiv - Biophysics 2021Quote: ... Human Gβ with a C-terminal 15-amino-acid polypeptide linker followed by a HiBiT (peptide 86, Promega) and human Gγ were cloned into pFastBac vector ...
-
bioRxiv - Microbiology 2023Quote: ... Standard curves were generated using purified genomic stocks (HSV-1 bacterial artificial chromosome and human genome Promega #G1471). Absolute copy number of genomic stocks was determined using droplet digital PCR (Biorad QX600) ...
-
bioRxiv - Neuroscience 2024Quote: ... human CaV1.3 cDNA (accession number NM_001128840.2) was de-novo synthesized and assembled into a HaloTag vector (Promega G7721) using restriction cloning ...
-
bioRxiv - Cell Biology 2019Quote: ... 1 μl of DNA was taken as template in a 25 μl PCR reaction using the Go Taq DNA polymerase (Promega) according to the provider’s recommendation ...
-
bioRxiv - Molecular Biology 2020Quote: ... 1 μL of lysate was diluted with 39 μL PBS before being mixed with 40 μL Dual-Glo FLuc substrate (Promega). After 10 min ...
-
bioRxiv - Molecular Biology 2021Quote: ... 5 µl of the reaction was treated with 1 µl of RNase mixture (10% RNase A, Sigma + 20% RNase One, Promega), incubated at 37°C for 10 min and then mixed with 6 µl of 2 × SDS-PAGE buffer ...
-
bioRxiv - Immunology 2022Quote: ... injecting 50 μl per well of coelenterazine substrate (Nanolight Technologies, 2 μg/ml) and analysing luminescence on a FLUOstar OPTIMA luminometer (Promega). Data were normalised to a mock-treated control to generate a fold induction ...
-
bioRxiv - Molecular Biology 2019Quote: ... 5 μl of Reverse Transcription Mix (10 μl of 10 mM dNTPs and 40 μl of 5X MMLV RT Buffer Promega), 1 μl of 10 mM ddTTP for the sequencing reaction and 1 μl of MMLV Reverse Transcriptase RNAse H minus (Promega ...
-
bioRxiv - Molecular Biology 2019Quote: ... 1 μl of 10 mM ddTTP for the sequencing reaction and 1 μl of MMLV Reverse Transcriptase RNAse H minus (Promega) were finally added and the reverse transcription was performed at 35°C for 2 minutes ...
-
bioRxiv - Plant Biology 2019Quote: ... the coding sequences of bes1-D(L) from cDNA of the bes1-D mutant and RLUC from the vector pRL vector (Promega) were amplified by PCR using the following primers:
-
bioRxiv - Biochemistry 2020Quote: ... Gel pieces were cooled on ice and rehydrated with 100 μ L of 10 ng/μL Trypsin Gold (Promega, UK) for 5 min ...