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Citations for Promega :
3101 - 3150 of 3631 citations for Human Gastric Intrinsic Factor GIF CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... The PCR product was extracted from 2% agarose gel and purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, USA) according to the manufacturer’s instructions and quantified using a Quantus Fluorometer (Promega, USA). Normalization was performed the counts of individual OTUs in a sample by dividing the total counts of all OTUs within that sample followed by a multiplication by resulting in relative abundance expressed.
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bioRxiv - Neuroscience 2023Quote: Approximately 5x104 HEK293 cells were seeded on poly-L-lysine coated glass coverslips and transfected with expression constructs for SORL1-WT or SORL1-R953C using Fugene 6 Transfection Reagent kit (Promega). 24h post-transfection ...
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bioRxiv - Immunology 2022Quote: ... Cells were immediately stimulated with cytokine for 24 hr and nanoluciferase activity was measured relative to control activity using the NanoGlo Dual Luciferase kit (Promega). The primers for cloning enhancer fragments into pNL3.1 are in Table S3.
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bioRxiv - Immunology 2023Quote: ... total RNA was purified from 3-4 tissues of female flies using a ReliaPrep RNA Tissue Miniprep kit (Promega, z6112). Quantitative RT-PCR was performed using a OneTaq RT‒PCR kit (Promega ...
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bioRxiv - Molecular Biology 2022Quote: ... the luminescence of 50 µl of cell lysate was measured using the Nano-Glo Luciferase Assay kit (Promega, Madison, WI) as per the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... All VLP volumes were calculated and normalized to 1.4 x 107 RLU using the Nano-Glo® HiBiT Lytic Detection System kit (Promega).
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bioRxiv - Microbiology 2022Quote: ... 48 h post transfection cells were harvested for western blotting or luminescence detection using a Firefly Luciferase Assay kit (Promega) and a Glomax luminometer (Promega) ...
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bioRxiv - Immunology 2023Quote: ... Luciferase and Renilla signals were measured 48 h after transfection by a Dual-Luciferase Reporter Assay Kit (Promega, No. E1980). Data were analyzed and normalized by the division of firefly luciferase activity with that of Renilla luciferase to eliminate transfection efficiency difference.
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bioRxiv - Immunology 2023Quote: ... viral RNA (vRNA) was isolated from plasma samples using the Maxwell Viral Total Nucleic Acid Purification kit (Promega, Madison WI). Next ...
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bioRxiv - Microbiology 2023Quote: ... Caspase 3/7 enzymatic activity in raw cell lysates was measured using a Caspase Glo 3/7 assay kit (Promega) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2023Quote: ... Cells were pelleted (2000 g x 5 min) and subjected to genomic DNA (gDNA) extraction using the Wizard Genomic DNA Purification kit (Promega), according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2023Quote: ... higginsianum genomic DNA used as PCR templates were isolated by Maxwell RSC Plant DNA Kit with Maxwell RSC 48 instrument (Promega) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: 5,000 indicated cells were seeded in each well of 96-well plates followed by treatment with indicated doses of compounds for 72 hrs before cell proliferation analysis by Cell Titer-Glo Kit (G9241, Promega) according to manufacture instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The mixture was incubated at 25°C for 30 minutes and the production of ADP was measured using the ADP-Glo kinase assay kit (Promega). Vmax was calculated after fitting the kinase activity data to the Michaelis–Menten equation ...
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bioRxiv - Plant Biology 2023Quote: ... Infiltrated leaves were harvested 48 hrs after infiltration and luciferase activity was measured immediately using the Dual-Glo Luciferase Assay kit (Promega). Leaf disks 4 mm in diameter were cut and inserted into white 96 well plates with 75 µl of 1x PBS and 75 µl of luciferase assay reagent ...
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bioRxiv - Plant Biology 2023Quote: ... Five micrograms of total RNA were ligated to the RNA GeneRacer oligo adapter and subjected to reverse transcription utilizing Improm-II Reverse Transcriptase kit (Promega). The cDNA was used for amplification of cleaved PYL6 fragments ...
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bioRxiv - Genetics 2023Quote: ... After a second purification using a Zymo DNA Clean and Concentrator kit the PCR product was used as a template in a T7 RiboMAX express RNAi System (Promega) following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... Purified DNA was run on 2% agarose gel and bands corresponding to ∼150 bp were cut and purified with a Gel Purification kit (Promega). Libraries were constructed using the Nugen Ovation Ultralow Library System V2 following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... Translation reactions were incubated for 23 min at 30 °C and nanoluciferase activity was monitored using the Nano-Glo Luciferase Assay Kit (Promega) in a Microplate Luminometer (Veritas) ...
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bioRxiv - Cell Biology 2023Quote: ... Other parameters of ER Stress induced cell death were measured through immunoblotting or with the Caspase 3/7 Glo Assay Kit (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... The ratios of luciferase activity for the renilla and firefly luciferases were measured 48 hrs (hours) after transfection using the dual- Luciferase reporter assay kit from Promega and a luminometer ...
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bioRxiv - Microbiology 2023Quote: ... and genomic DNA was isolated from overnight cultures (5 mL) using the Wizard Genomic DNA Purification Kit (Promega GmbH, Walldorf) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: RT-qPCRs were performed with MLV SU or 2-LTR primers (Table 1) using a Power SYBR green PCR kit (Promega) and the QuantStudio 5 real-time PCR system (Applied Biosystems) ...
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bioRxiv - Microbiology 2023Quote: Genomic extraction for the WT C6706:str2 strain and each of the resistant mutants was performed using Wizard Genomic DNA Purification Kit (Promega). The concentration of DNA was quantified using Nanodrop spectrophotometer (ND-1000) ...
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bioRxiv - Zoology 2023Quote: Total RNA was extracted using the Maxwell 16 LEV Plant RNA Kit with the Maxwell 16 Research Instrument (Promega, USA) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The bacterial cells were collected from 2mL of the culture and the genomic DNA was extracted using the Wizard Genomic DNA Purification Kit (Promega). Samples were then sent to Novogene Co. ...
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bioRxiv - Cancer Biology 2023Quote: ... Phenol-chloroform phase separation and chloroform phase separation steps were repeated to remove the RNase and then DNA was purified from the aqueous phase by using Promega kit protocol (Promega Wizard SV Gel and PCR Clean-Up System ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was isolated with the Maxwell RSC viral total nucleic acid purification kit on a Maxwell RSC 48 instrument (Promega). Isolated ZIKV RNA was titrated by qRT-PCR using TaqMan Fast virus 1-step master mix (ThermoFisher ...
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bioRxiv - Cell Biology 2023Quote: ... and used as template to generate dsRNA in vitro using the Promega T7 RibomaxTM Express RNAi System kit (Promega, P1320). The dsRNA was precipitated with sodium acetate (300 mM final ...
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bioRxiv - Biochemistry 2023Quote: ... HiBit and firefly luciferase activity were measured simultaneously using a Nano-Glo HiBiT Dual-Luciferase Reporter System kit (Promega, N1630) with purified LgBiT Protein (Promega) ...
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bioRxiv - Biochemistry 2023Quote: ... PCR reaction mixtures were solved in 1% (w/v) agarose gel and purified using Wizard SV gel & PCR Clean-Up kits (Promega). Then ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with or without 50 μM adenosine 5′-triphosphate (ATP) at 30 °C for 30 minutes followed by kinase assays using ADP-Glo Kinase Assay kit (Promega). Data were analyzed using GraphPad Prism 9 ...
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bioRxiv - Cancer Biology 2022Quote: ... for 72 hours and cell proliferation was assayed using the CellTiter 96 Non-Radioactive Cell Proliferation kit and a GloMax Discovery plate reader (Promega). For proteomic mass spectrometry ...
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bioRxiv - Cancer Biology 2023Quote: ... All cell lines were validated by DNA fingerprinting through the University of Colorado Molecular Biology Service Center utilizing the STR DNA Profiling PowerPlex-16 HS Kit (DC2101, Promega)(Extended Data Figure 11).
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bioRxiv - Genomics 2023Quote: DNA was extracted from the agar/cell mixture from the output and control pools using a Wizard Genomic DNA Purification Kit (Promega). The manufacturer’s protocol was followed with two modifications ...
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bioRxiv - Biochemistry 2023Quote: ... Firefly and Renilla luciferase activities were measured using a dual-luciferase reporter assay kit and a GloMax 20/20 Luminometer (Promega). Transfection efficiency was normalized to that of Renilla luciferase ...
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bioRxiv - Biochemistry 2023Quote: ... U87MG cells were authenticated within a year of their use by the Johns Hopkins Genetics Resources Core Facility by short tandem repeat analysis using the PowerPlex 16 HS kit (Promega). Unless otherwise indicated ...
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bioRxiv - Microbiology 2023Quote: Genomic DNAs were purified from 600 µL of overnight bacterial culture using the Wizard HWM genomic DNA prep kit (Promega) following the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: Cell viability was measured using a commercial kit (Cell Titre 96 AQueous One Solution cell proliferation assay; Promega, Madison, USA) that uses a tetrazolium salt-based colorimetric assay [20] ...
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bioRxiv - Synthetic Biology 2023Quote: ... 10 μL of stopped reaction samples were mixed with 50 μL of luciferase substrate solution (Luciferase Assay System kit, Promega) in a white flat bottom 96-well microplate ...
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bioRxiv - Plant Biology 2023Quote: ... Total RNA was extracted from backup samples for transcriptome analysis using Eastep® Super Total RNA Extraction Kit (Promega, USA). Reverse transcription was performed using GoScriptTM Reverse Transcription System (Promega ...
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bioRxiv - Evolutionary Biology 2023Quote: ... the bands were excised from the gel and TA cloned using the pGEM- T easy-vector II cloning kit (Promega). The standard mini-prep step was avoided by using colony PCR ...
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bioRxiv - Microbiology 2023Quote: ... Gene expression was assessed by qPCR using primers listed in the supplemental material Table S6 and the GO Taq PCR kit (Promega). Gene expression was quantified using SYBR Green (Roche ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Quality was assessed with an Agilent Bioanalyser and the quantity was calculated by an RNA-specific kit from Quantus (Promega).
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bioRxiv - Plant Biology 2023Quote: ... Transcription factors were expressed using at least 2000 ng PCR product per sample with the TnT T7 Quick for PCR DNA in vitro protein expression kit (Promega). All reaction volumes were doubled to yield a total of 100 µL protein product per transcription factor ...
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bioRxiv - Microbiology 2023Quote: High-molecular weight genomic DNA was isolated based on a modified version of Promega’s Genomic DNA Isolation Kit (Promega, A1120) [63] ...
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bioRxiv - Plant Biology 2023Quote: Total RNA was extracted from frozen ground tissues using the innuPREP Plant RNA kit (Analytic Jena) and digested with RQ1 DNase (Promega). First-strand cDNA synthesis was conducted on 1 µg RNA using the SuperScript III Reverse Transcriptase (Invitrogen ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted using E.Z.N.A.® Plant RNA Kit (R6827-01, OMEGA) following the manufacturer’s instructions and treated with RNase-Free DNase (M6101, Promega) to remove genomic DNA contamination ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell viability was assayed starting at Day 1 for 6 days according to the CellTiter-Glo 3D kit (Promega G9683). For the antiandrogen response assay ...
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bioRxiv - Microbiology 2023Quote: ... Total genomic DNA was extracted from the mid-log culture using the Wizard® Genomic DNA Purification Kit from Promega following the manufacturer’s protocol.