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Citations for Promega :
3051 - 3100 of 3874 citations for Mouse Chitinase 1 CHIT1 Protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... One μg of mass spectrometry grade trypsin (Trypsin Gold, Promega; 1 mg/mL in 0.03% acetic acid) was added ...
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bioRxiv - Molecular Biology 2024Quote: ... Digestion buffer (50 mM TEAB pH 8.0) containing sequencing-grade modified trypsin (1/25, w/w; Promega) was added to the S-trap column and incubate for 1h at 47 °C ...
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bioRxiv - Plant Biology 2024Quote: ... The secondary antibody (Goat anti-rabbit IgG HRP, Promega, diluted 1:133333 in 5% milk in PBS) was then applied for 50 minutes ...
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bioRxiv - Biophysics 2024Quote: ... Detection reagent was prepared by 1:25 dilution of the furimazine NanoGlo Live Cell Substrate (LCS) (Promega) into a 1:1 mixture of phosphate-buffered saline (ThermoFisher ...
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bioRxiv - Genomics 2024Quote: ... The CaGHV-1 ORF50 promoter fragments were PCR amplified and cloned into pGL4.15 luciferase reporter vector (Promega) using In-Fusion cloning ...
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bioRxiv - Cell Biology 2024Quote: ... Overnight digestion of the sample was performed using 2.5 μl of 1 μg/μl trypsin (Promega V528A) at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... Reverse transcription was performed on 1 μg of RNA with random hexamer primers using MMLV-RT enzyme (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 μg of DNA-free RNA was then used for reverse transcription using M-MLV Reverse Transcriptase (Promega) in the presence of RiboLock ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Sperms were released from antheridiophores by applying ddH2O supplemented with RNasin® Ribonuclease Inhibitor (1 u/μl, Promega), collected in a 1.5 mL tube ...
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bioRxiv - Pathology 2022Quote: ... A total of 1 µg of extracted RNA was transcribed into cDNA using GoScript Reverse Transcription Kit (Promega). Quantitative real-time PCR was performed using FastStart SYBR Green Master mix (Roche ...
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bioRxiv - Neuroscience 2022Quote: ... The sample solutions were diluted to 1 M urea with 50 mM ammonium hydrogen carbonate and trypsin (Promega) was added at an enzyme-to-substrate ratio of 1:50 ...
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bioRxiv - Neuroscience 2021Quote: ... cDNA synthesis was subsequently performed using 1 μg total RNA and oligo-dT primers (C110A, Promega, Madison, WI). Transcript levels were assessed via real-time quantitative polymerase chain reaction (qPCR ...
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bioRxiv - Molecular Biology 2020Quote: Transfection of 1 µg of each individual DNA was performed in six-well plates using FuGENE HD (Promega) for HeLa S3 cells according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2020Quote: Cells were washed twice with PBS then lysed directly in wells using 1× cell culture lysis reagent (Promega). Cell lysates were clarified by centrifugation ...
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bioRxiv - Microbiology 2020Quote: ... Samples were diluted with three volumes digestion buffer (100 mM Tris/HCl pH 8.5, 1 mM CaCl2) and 0.25 μg Trypsin (Sequencing Grade, Promega) were added and incubated o/n at 37 °C ...
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bioRxiv - Neuroscience 2020Quote: ... pCAG-DCC:TDTOMATO and pCAG-DCCkanga:TDTOMATO plasmids (1 µg) were transfected into the plated U251 cells using FuGENE 6 (Promega) in Opti-MEM (Gibco ...
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bioRxiv - Bioengineering 2020Quote: ... Total RNA was reverse transcribed using 0.5–1 µg of total RNA and ImProm-II Reverse Transcriptase (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2020Quote: The Round 1 eluted pool was PCR amplified for 10 cycles with GoTaq Hot Start Polymerase (Promega, M5001) (1X Colorless GoTaq Flexi Buffer ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was diluted 1:5 before quantitative analysis by qPCR was done using GoTaq qPCR Master Mix (Promega) following manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2022Quote: ... The eluent was equally divided into three for digestion with either: 33:1 (w/w) trypsin gold (Promega), 25:1 (w/w ...
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bioRxiv - Biochemistry 2022Quote: ... followed by the secondary antibody for 1 h at +4 °C (anti-rabbit IgG-HRP, Promega 65-6120). The Pierce® enhanced chemiluminescence substrate (Thermo-Fischer Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... The urea concentration was then diluted with 17 μl of 50 mM HEPES pH 8.5 and after adding 1 μl of 0.2 mg/ml trypsin (Promega) in 0.2 mM HCl the digestion continued overnight at 37°C with interval mixing at 2000 RPM for 30 s every 15 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... pMD2.G and the lentiviral gRNA plasmid at a 3:1:5 mass ratio using FuGENE HD (Promega) in Opti-MEM (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2021Quote: ... filled with 175 μL 0.5x MS-agar medium 1% sucrose and 35μL (50 μg/mL) of Beetle Luciferin (Promega) dissolved in DMSO ...
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bioRxiv - Molecular Biology 2020Quote: ... 293T cells were transfected in 12 well plates with 1 ug of pcDNA3.4 POLGARF or pcDNA3.4 Timer (as a negative control) with Fugene transfection reagent (Promega) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... were transfected with 4.5 µg of the pBabe-puro-NCAM140 mApple construct (NCBI reference sequence: NM_000615.6, transcript variant 1) using Fugene 6 Transfection Reagent (Promega). Supernatant containing viral particles was collected and concentrated using PEG-IT (System Biosciences ...
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bioRxiv - Biochemistry 2021Quote: ... The sample was four-fold diluted and the protein digested in presence of 0.2 M Tris-HCl buffer pH 8.0 and 1 μg of sequencing grade trypsin (Promega) previously dissolved in 20 mM acetic acid ...
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bioRxiv - Biochemistry 2020Quote: ... and added to 50-100 µL ribosome-coupled anti-Flag M2 agarose beads and 1 µL RNasin (Promega) per reaction ...
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bioRxiv - Neuroscience 2022Quote: ... then digested at a 1:25 enzyme:protein ratio with proteomics grade trypsin/rLysC (V5071; Promega, Madison, WI, USA) overnight at 37°C according to the SP3 method (Hughes et al ...
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bioRxiv - Cancer Biology 2022Quote: ... The first step of PCR was carried out with 1 µl of cDNA and DNA polymerase GoTaq (Promega) according to the manufacturer’s instructions and consisted of 95 °C for 5 min and 15 cycles of 95 °C for 30 seconds ...
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bioRxiv - Cell Biology 2020Quote: DNA from DLD-1 and nucleoporins-targeted cells was extracted with Wizard® Genomic DNA Purification Kit (Promega). Clones were genotyped by PCR for homozygous insertion of tags with two sets of primers listed below.
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bioRxiv - Synthetic Biology 2020Quote: ... a 1 mL aliquot was taken for genomic isolation through the Maxwell kit (Promega, Cat# AS1490, Madison WI). Successful integration was verified through qPCR ...
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bioRxiv - Synthetic Biology 2020Quote: ... a 1 mL aliquot was taken for genomic isolation using the Maxwell kit (Promega, Cat# AS1490, Madison WI). Successful integration was verified using qPCR ...
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bioRxiv - Microbiology 2020Quote: ... A DENV-1 3’UTR specific probe was generated by PCR reaction with GoTaq Polymerase (Promega, Wisconsin, USA) containing DIG DNA-labelling mix (Roche ...
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bioRxiv - Molecular Biology 2020Quote: ... 8 μl 5x PNK pH 6.5 buffer [350 mM Tris-HCl pH 6.5, 50 mM MgCl2, 5 mM DTT], 1 μl PNK enzyme [NEB], 0.5 μl RNasin [Promega] ...
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bioRxiv - Plant Biology 2021Quote: ... The powder was resuspended in 30 mL of Extraction Buffer 1 (10mM Tris-HCl pH8; 0.4M sucrose; 10 mM MgCl2; 5 mM BME; 0.2 mM PMSF; RNasin PROMEGA) and was filter thought a Nylon membrane of 150 μm and centrifuged at 2000 g for 20 min at 4 °C ...
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bioRxiv - Immunology 2020Quote: ... Cells were then incubated with CellTiter 96 Aqueous One Solution Proliferation Solution for 1 hour (Promega, Fitchburg, WI). Absorbance of the solution was then read at 490 nm ...
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bioRxiv - Biochemistry 2020Quote: ... The eluent was equally divided into three for digestion with either: 10:1 (w/w) Trypsin Gold (Promega), 7.5:1 (w/w ...
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bioRxiv - Immunology 2020Quote: ... the membrane was incubated with HRP-conjugated goat anti-rabbit IgG diluted 1:2000 (Promega, Madison, Wisconsin, USA) for 2 h at RT ...
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bioRxiv - Immunology 2021Quote: ... qRT-PCR was performed in 12 μL reactions using GoTaq Probe 1-step RT-qPCR System (Promega, US) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ATP release from THP-1 cells was determined by the ENLIGHTEN ATP Assay System Bioluminescence Detection Kit (Promega). Cells were grown in 6-well plates and starved overnight ...
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bioRxiv - Cancer Biology 2021Quote: ... and sequentially digested with Lys-C (Wako) (protein:enzyme ratio 1:50, o/n at RT) and trypsin (Promega) (protein:enzyme ratio 1:50 ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 μg of DNA-free RNA was then used for reverse transcription using M-MLV Reverse Transcriptase (Promega) in the presence of RiboLock ...
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bioRxiv - Cell Biology 2021Quote: ... was then used to transcribe 1 µg of total RNA to cDNA using Oligo(dT)15 primers (Promega). KAPA Probe Fast qPCR Master Mix (2X ...
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bioRxiv - Molecular Biology 2021Quote: 1 uL of pUG cDNA was then input into a 20 uL GoTaq PCR reaction (Promega; Cat # M7123) with the first adapter specific primer (Shukla et al. ...
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bioRxiv - Immunology 2020Quote: ... the indicated NLRP1 construct (0.02 μg) and RFP (to 1 µg) with FuGENE HD according to manufacturer’s instructions (Promega). At 24 h cells were treated with DMSO or VbP (10 µM) ...
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bioRxiv - Cancer Biology 2020Quote: pET20b-6xHis-ARF5D17 or pGEX-5x-1-hGGA3 [VHS-GAT] were transformed into BL21 (DE3) pLysS bacteria (Promega). Single colonies were grown in lysogeny broth (LB ...
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bioRxiv - Cell Biology 2020Quote: ... Isolated RNA (1 μg) was used to synthesize cDNAs using Murine Moloney Leukemia Virus reverse transcriptase (MMLV, Promega). Subsequently ...
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bioRxiv - Immunology 2022Quote: ... Cell pellets were solubilized with 10 μL of 8M urea and 10 μL of 1% Protease MAX (Promega) in 100 mM Tris-HCl ...
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bioRxiv - Microbiology 2023Quote: Real-time PCR was carried out using the Promega GoTaq® Probe 1-Step RT-qPCR System (Promega) with Sarbecovirus-specific envelope gene primers from bat RNA samples as published by (Corman ...