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Citations for Promega :
3001 - 3050 of 3874 citations for Mouse Chitinase 1 CHIT1 Protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... 1 µL of gDNA was added to a standard 24 µL 2X GoTaq Green PCR (Promega, #M7123), typically ∼35 cycles ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Due to higher incubation temperatures we used 1 μL RNasin Plus Ribonuclease Inhibitor (Promega, Madison, WI, USA). All other methods were identical to previous reverse transcriptions with the exception of changing the main incubation step to 55°C for 1 hour in order to disrupt RNA secondary structure ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA was produced from 1 μg of RNA sample using M-MLV reverse transcriptase (Promega; M-1302). Real time qPCR was preformed using a QuantiNova SYBR Green kit (Qiagen ...
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bioRxiv - Genetics 2020Quote: ... 16 µL of a 1:100 dilution (in 25 mM ammonium bicarbonate) of trypsin solution (Promega V511C) was added ...
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Mucociliary Clearance Augmenting Drugs Block SARS-Cov-2 Replication in Human Airway Epithelial CellsbioRxiv - Microbiology 2023Quote: ... ARINA-1 cytotoxicity was measured using the CellTiter-Glo® 2.0 Cell Viability Assay (Promega, Cat. # G9241), which measures intracellular ATP ...
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bioRxiv - Microbiology 2023Quote: ... 1–2 μg of each RNA was treated with RNase-free Dnase I (Promega, Madison WI, USA) and reverse transcribed using the PrimeScript RT reagent Kit (TaKaRa) ...
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bioRxiv - Microbiology 2023Quote: ... After the addition of 1x Nano-Glo Lysis Buffer and 1:1000 Nano-Glo substrate (Promega, USA), relative light units (RLU ...
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bioRxiv - Bioengineering 2023Quote: ... cells were treated with the CellTiter-Glo 2.0 Viability Assay (1:2 v/v; Promega, Madison, WI) using the Viaflo Assist Pipetting Platform (Integra Biosciences ...
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bioRxiv - Neuroscience 2023Quote: ... Gel bands were washed in 100 mM AmBic/ACN prior to adding 1 µg trypsin (Promega #V5111) for overnight incubation at 37°C ...
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bioRxiv - Bioengineering 2023Quote: ... 250 ng to 1 μg of RNA were reverse transcribed using M-MLV Reverse transcrtiptase (Promega, #M1705) and oligo(dT ...
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bioRxiv - Neuroscience 2023Quote: ... Gel bands were washed in 100 mM AmB/ACN prior to adding 1 µg trypsin (Promega #V5111) for overnight incubation at 37°C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... at a ratio of 1:166 to Opti-MEM media in combination with extracellular NLuc Inhibitor (Promega) diluted 1:500 (10 μL of 30 mM stock per 5 mL Opti-MEM plus substrate ...
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bioRxiv - Cancer Biology 2023Quote: ... ZR-75-1 cells were short tandem repeat tested every 4 months (Stem Elite ID System, Promega). Doxorubicin (S1208) ...
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Pan-tissue mitochondrial phenotyping reveals lower OXPHOS expression and function across tumor typesbioRxiv - Biochemistry 2023Quote: ... 1mM CaCl2 for overnight digestion via trypsin at 32°C (Promega, Cat# V5113; 1:50 w:w enzyme:protein). Following digestion ...
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bioRxiv - Cell Biology 2024Quote: ... at a ratio of 1:166 to Opti-MEM media in combination with extracellular NLuc Inhibitor (Promega) diluted 1:500 (10 µL of 30 mM stock per 5 mL Opti-MEM plus substrate ...
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bioRxiv - Microbiology 2024Quote: ... Then 20 µL of 50 mM ABC containing 1 µg of trypsin (Promega Platinum Mass Spectrometry Grade) was added and the traps were incubated 2h at 47°C in a covered thermomixer without vortexing ...
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bioRxiv - Genomics 2023Quote: ... 1 µg of Dnase-treated total RNA was reverse transcribed using the M-MLV Reverse Transcriptase (Promega) and Random Hexadeoxynucleotides (Promega ...
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Nucleolar Pol II interactome reveals TBPL1, PAF1, and Pol I at intergenic rDNA drive rRNA biogenesisbioRxiv - Molecular Biology 2023Quote: ... Tryptic digestion was performed by incubating beads with 1 μg MS-grade TPCK trypsin (Promega, Madison, WI) dissolved in 200 μl of 50 mM ammbic (pH 8.3 ...
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bioRxiv - Molecular Biology 2023Quote: ... Diluted RNA was assayed for AGO2 transcript using the GoTaq 1-Step RT-qPCR System (Promega A6020) with primers CTGGCTCCAGGGGACAAG (Forward primer ...
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bioRxiv - Cancer Biology 2022Quote: ... for 4h at 37C in 6M urea followed by an overnight trypsin digestion (1:25 ratio; Promega) at 37C in 1M urea ...
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bioRxiv - Genetics 2022Quote: ... Samples were diluted 1:4 with 50mM Tris/HCl (pH 8.0) and sequencing grade modified trypsin (Promega) was added in an enzyme-to-substrate ratio of 1:50 ...
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bioRxiv - Biophysics 2022Quote: ... PCR products were separated on 1% agarose gel and purified using a Gel extraction kit (Promega, CA). Sequences of forward and reverse primers are listed in Supplementary Table S21 ...
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bioRxiv - Biochemistry 2022Quote: ... urea was diluted to 1 M by adding 50 mM ammonium bicarbonate and 2 μg Trypsin (Promega) per 100 μg protein ...
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bioRxiv - Molecular Biology 2022Quote: Developing was performed using chemiluminescence reaction (ECL, GE-Healthcare) with secondary coupled to HRP (Promega, 1/5000e) and the Amersham ImageQuant 800 (Cytiva).
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bioRxiv - Molecular Biology 2023Quote: ... each TECprobe-ML reaction was 48 μl due to the omission of 10X (1 μM) streptavidin (Promega) and 10X Start Solution [100 mM MgCl2 ...
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bioRxiv - Biochemistry 2022Quote: ... pH 3.0) and mixed in a 40:1 molar ratio with commercially purchased pepsin protease (Promega, USA), then incubated at 37°C for 3 h and frozen until later use ...
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bioRxiv - Microbiology 2022Quote: ... viral RNA was quantified by RT-qPCR using the GoTaq Probe 1-Step RT-qPCR System (Promega) on a QuantStudio3 (ThermoFisher Scientific) ...
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bioRxiv - Biochemistry 2022Quote: ... the primary antibody was diluted in blocking buffer as follows: Anti-HaloTag (1:1000; Promega Corporation; G9281); anti-PHC1 antibody (1:500 ...
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bioRxiv - Bioengineering 2023Quote: ... 1 µg of total RNA was used to synthesize cDNA using M-MLV reverse transcriptase kit (Promega) using the supplier’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... The Nluc and BRET signals were measured (integration time: 1 s) after auto-injecting either Fz (Promega) or FFz (Promega ...
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bioRxiv - Immunology 2023Quote: ... 1 ng pEF1α-Renilla and 120 ng plasmid of interest complexed with 0.8 µl FuGENE HD (Promega) diluted to 10 µl total volume in OptiMEM ...
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bioRxiv - Biochemistry 2023Quote: ... and used 1 μL of cDNA for 10 μL of PCR using 2x GoTaq master mix (Promega) and 250 nM of forward and reverse primer sets with conditions recommended by the manufacturer ...
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bioRxiv - Cell Biology 2022Quote: Pulverized patient tissue samples were solubilized in 8M urea and 100 mM NaCl with 1% ProteaseMAX (Promega) in 100 mM ammonium bicarbonate (AmBic ...
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bioRxiv - Molecular Biology 2023Quote: ... and 1 μg total RNA was used to inverse transcribe to cDNA (Promega Biotech, Madison, WI, USA), which was subsequently used as templates ...
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bioRxiv - Plant Biology 2024Quote: ... alkylated (55 mM Iodoacetamide; 1h @ 23°C) and digested in solution with 1:100 trypsin (V5113; Promega). Peptides were acidified to 0.5% (v/v ...
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bioRxiv - Systems Biology 2024Quote: ... samples were diluted 1:4 with 50 mM Tris–HCl pH 8 and sequencing grade trypsin (Promega) was added at 1:50 ratio ...
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bioRxiv - Cell Biology 2024Quote: ... the reaction mixtures for in vitro transcription were treated with RQ-1 RNase-free DNase I (Promega). Synthesized mRNAs were treated with phenol–chloroform to remove protein components ...
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bioRxiv - Genomics 2024Quote: ... The master mixtures consisted of 1 x Taq&Go G2 Hot Start colorless PCR Master Mix (Promega), 0.5 μM of each primer ...
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bioRxiv - Molecular Biology 2024Quote: ... Developing was performed using chemiluminescence reaction (ECL, Cytiva) with secondary antibody coupled to HRP (Promega, 1/5000).
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bioRxiv - Microbiology 2024Quote: ... cDNA was reverse-transcribed from 1 µg of total RNA using IMPROM-II reverse transcriptase (Promega, A3803) with a random hexamer primer according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... that included (for 1 well): 5 μl of the SYBR Green Real-Time PCR master mix (Promega), 1.25 μl of the forward and reverse primer (standardized KiCqStartTM primers ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were adjusted to 3 mM EDTA and digested with 1 µg Trypsin/LysC mix (Promega, V5073) for 1 h at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... 1 µg of RNA was reverse transcribed via the GoScript™ Reverse Transcription System (Promega, Dübendorf, Switzerland) in a final volume of 20 µL.
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bioRxiv - Genomics 2024Quote: Up to 1 mL of DNA was extracted by the Maxwell RSC ccfDNA Plasma Kit (AS1480, Promega) with approximately 47 µL output ...
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bioRxiv - Immunology 2024Quote: ... 1 μg of total RNA was converted into cDNA using First Strand cDNA synthesis kit (M3682, Promega). Quantitative real-time RT-PCR was performed with SYBR Green PCR mixture (F416 ...
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bioRxiv - Plant Biology 2024Quote: ... 1 µg or total RNA was used for the cDNA synthesis (GoScript ™ Reverse Transcription System, Promega). Quantitative real-time PCRs were performed in the Agilent AriaMx System using the GoTaq® qPCR Master Mix (Promega ...
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bioRxiv - Microbiology 2024Quote: ... viral RNA was reverse transcribed and quantified using GoTaq Probe 1-Step RT-qPCR System kit (Promega) in the presence of the specific SARS-CoV-2 probe (5′-6-FAM-ACT TCC TCA AGG AAC AAC ATT GCC A-BHQ-1-3′ ...
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bioRxiv - Molecular Biology 2024Quote: ... 50ul (50.000 cells) were transferred to a new 1.5ml Eppendorf tube and 0.5ul 1% Digitonin (Promega #G9441) was added by pipetting well few times ...
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bioRxiv - Synthetic Biology 2024Quote: ... after which they were treated with 1 µL RNase A (4 mg/mL RNAse A solution, Promega), and 1 µL RNase One (10 units/µL RNase ONE Ribonuclease solution ...
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bioRxiv - Genomics 2024Quote: ... the final selection was performed by 3% agarose electrophoresis (80 V, 2 h, 1 kB Promega ladder). The selected clones were preincubated in growth medium (15 ml ...