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Citations for Promega :
3051 - 3100 of 5099 citations for 7 hydroxy 1H 1 2 4 triazolo 1 5 a pyrimidin 5 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... cell viability was assessed using the MTS assay (CellTiter 96® AQueous One Solution Cell Proliferation, G3580, Promega, Madison, WI, USA). In brief ...
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bioRxiv - Genomics 2023Quote: ... TRs were amplified using the forward and reverse primers plus an M13 primer with one of the four fluorophores with GoTaq polymerase (Promega #PRM7123) using PCR program ...
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bioRxiv - Biophysics 2023Quote: To investigate CBD influence on the cells viability and metabolic activity the cells were tested using CellTiter 96® AQueous One Solution Cell Proliferation Assay (Promega) with tetrazolium compound ...
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bioRxiv - Molecular Biology 2023Quote: ... Cell viability was measured 72 hr after drug treatment using the CellTiter 96® AQueous One Solution Cell Proliferation Assay (Promega) following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2024Quote: Cytotoxic activity of the CAR T cells was evaluated with the Promega ONE-Glo™Luciferase Assay (Promega Corporation; cat# E6120) according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... The MTS assay was carried out at certain time points using The CellTiter 96 AQueous One Solution Cell Proliferation Assay (Promega #G3580) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... cells were gently washed once with 1X DPBS and MTS reagent of 20 μL (Cell Titer-96 Aqueous One Solution, Promega, USA) in 80 μL of cell culture media for 3 h (37°C and 5% CO2) ...
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bioRxiv - Cell Biology 2020Quote: ... 2 µl ViaFect™ (Promega, Madison, WI) and 40 µl Gibco Opti-MEM reduced serum media (Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: The psiCHECK-2 vector (Promega, Cat #C8021) was used to construct plasmids for dual-luciferase reporter assay ...
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bioRxiv - Cell Biology 2022Quote: ... 2 mg/ml proteinase K (Promega, v3021)) at 37 °C overnight followed by addition of 10 μl of 3M potassium acetate and incubation at room temperature for 1h ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 µg of sequencing grade Trypsin (Promega) was added ...
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bioRxiv - Microbiology 2020Quote: ... and 2 μL RNasin RNase inhibitor (Promega). The transcription reaction was incubated overnight at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... and 2 μg Sequencing Grade Trypsin (Promega). The beads were shaken overnight at 37 °C and pelleted by centrifugation (1,400 rcf ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 µg of sequencing grade trypsin (Promega) was then added to the samples and they were digested overnight at 37°C ...
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bioRxiv - Microbiology 2022Quote: The psiCHECK-2 control vector (Promega, C8021) and the psiCHECK-RL-Ifnb1 3′ UTR vector were described before26 ...
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bioRxiv - Microbiology 2021Quote: ... 2 µL dNTP (Promega U151B, 2.5 mM), 1.5 µL MgCl2 (25 mM) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 0.05 μg LgBiT-β-arrestin-2 (Promega) plus 0.9 µg pcDNA3.1 for 24 hours ...
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bioRxiv - Microbiology 2020Quote: ... 2 μL of luciferin reagent (Promega BrightGlo) was added and luciferase activity detected (Perkin Elmer Envision) ...
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bioRxiv - Bioengineering 2022Quote: ... 2 U/µL RNasin Ribonuclease Inhibitor (Promega), 1.2 µM PrimeTime primers ...
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Optimization and deoptimization of codons in SARS-CoV-2 and the implications for vaccine developmentbioRxiv - Evolutionary Biology 2022Quote: Dual-luciferase assay (psiCHECK-2 vector, Promega) was selected to analyze the effects of the viral mutation on protein synthesis ...
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bioRxiv - Molecular Biology 2024Quote: ... and digested with 2 µg Trypsin (Promega) overnight at 37 °C shaking at 1200 RPM on a thermomixer ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We then added 2 μg trypsin (Promega) in 100 mM TEABC and incubated overnight at 30 °C ...
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bioRxiv - Microbiology 2023Quote: ... and 2× GoTaq qPCR Master Mix (Promega). qPCR was performed using the MyGo Pro real-time PCR instrument (IT-IS Life Science Ltd.) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... containing a 2× concentration of Endurazine (Promega) was added to each well ...
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bioRxiv - Immunology 2020Quote: ... The following day cells were transfected with plasmids encoding for FLAG-tagged NLRP1 (2 μg) or the indicated DPP9 construct (2 µg) with FuGENE HD according to manufacturer’s instructions (Promega). Cells were harvested ...
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bioRxiv - Molecular Biology 2023Quote: ... precipitated proteins were eluted by incubation of beads with Elution Buffer (2 M Urea, 50 mM Tris pH 7.5, 2 mM DTT, 20 µg/ml Trypsin Gold (Promega)) for 30 min at 37°C shaking at 1,400 rpm in the dark ...
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Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... COS-7 cells were plated on 10 cm plates and transfected 24h prior to lysis using FuGENE 6 (Promega; 6-12 µg total DNA). For siRNA tests ...
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bioRxiv - Cell Biology 2023Quote: ... COS-7 cells were plated on 10 cm plates and transfected 24h prior to lysis using FuGENE 6 (Promega; 6-12 µg total DNA). Cells were routinely tested for mycoplasma using a MycoAlert detection kit (Lonza ...
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bioRxiv - Cancer Biology 2021Quote: ... at 1:166 to Opti-MEM media in combination with extracellular NanoLuc Inhibitor (Promega) diluted 1:500 was combined to create a 3X stock solution ...
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bioRxiv - Cell Biology 2020Quote: ... We used the following primary antibodies: mouse anti-β-galactosidase (Promega, 1:1,000 dilution), rabbit anti-GFP (MBL,1:1,000 dilution) ...
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The key features of SARS-CoV-2 leader and NSP1 required for viral escape of NSP1-mediated repressionbioRxiv - Molecular Biology 2021Quote: ... 100 ng of Trypsin Gold and 1 μg of Trypsin sequenced grade (both Promega) were added to BioID eluates and total cell lysates ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and in bioluminescence mode while immersed in NanoGlo substrate (diluted 1:50, Promega #N1110). Tissues were homogenized in 1 ml N-PER (brain ...
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bioRxiv - Molecular Biology 2022Quote: ... The proteins were digested in 1 M urea with modified trypsin (Promega, Madison, WI) at a 1:50 enzyme-to-substrate ratio ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were then harvested and lysed with 25μL of 1×passive lysis buffer (Promega) for 30 min at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were transfected with 1 µg DNA and 3 µl FuGENE HD (Promega, E2311) in 150 µl Opti-MEM media according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... Horseradish peroxidase-conjugated goat anti-mouse or goat anti-rabbit IgG (Promega; 1:10,000) was incubated for 1 h at room temperature and then washed four times with PBS-Tween for 20 min ...
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Multiple steps mediate ventricular layer attrition to form the adult mouse spinal cord central canalbioRxiv - Developmental Biology 2019Quote: ... Probe was detected using alkaline phosphatase-labelled anti-digoxigenin antibody (1:1000, Promega, 11093274910) in blocking buffer at 4°C overnight ...
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bioRxiv - Plant Biology 2019Quote: ... The secondary antibody was an anti-mouse coupled to HRP 1:10,000 (W4021, Promega). Detection was performed with the Pico Plus system (34580 ...
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bioRxiv - Biochemistry 2019Quote: ... The plasmid pGEM-1 and the TNT SP6 Transcription/Translation System were from Promega. [35S]Met was from PerkinElmer.
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bioRxiv - Cancer Biology 2019Quote: ... 1 μg of RNA was reversed transcribed using cDNA synthesis reagents (Promega, Madison, WI). qPCR was performed using 6-carboxyfluorescein labeled TaqMan probes (Thermo ...
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bioRxiv - Genetics 2021Quote: ... 1 μg linearized vector was purified with the PureYield Plasmid Miniprep System (Promega, A1223) and used as template for in vitro synthesis of capped mRNA with the Ambion mMessage Machine T3 Transcription Kit (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: ... USA) and 10 μl of GoTaq 1-Step RT-qPCR (Promega, Madison, WI, USA). Thermal cycling was performed at 50°C for 15min for reverse transcription ...
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bioRxiv - Microbiology 2021Quote: ... RT-qPCR was performed using the GoTaq® 1-Step RT-qPCR System (Promega). Knockdowns were verified using the primers ...
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bioRxiv - Biochemistry 2020Quote: ... Proteomic digestion was performed by the addition of 1 μg proteomics-grade Trypsin (Promega) and incubated overnight at 37 °C ...
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bioRxiv - Biophysics 2021Quote: ... 20μL of a 1/5th diluted solution of Bright-Glo reagent (Promega, Madison, WI) was added directly to the wells ...
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bioRxiv - Neuroscience 2021Quote: ... USA) at 1:10 dilution and polyclonal HaloTag antibody (Promega Corp. Madison, WI, USA) at 1:500 dilution and FITC-conjugated donkey anti-mouse secondary antibody and Cy3-conjugated goat anti-rabbit secondary antibody (Invitrogen).
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bioRxiv - Cell Biology 2021Quote: ... 9 μl purified RNA was incubated with 1 μl Oligo(dT)15 primers (Promega) at 80°C for 3 min ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by an overnight trypsin digestion (ratio 1:33 enzyme:lysate, Promega, Madison, WI, USA).
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bioRxiv - Cancer Biology 2020Quote: ... Plates were incubated for 1 h at RT and luminescence recorded (Glowmax Luminometer, Promega) and then compared to Glucose/Lactate standards to determine relevant concentrations.
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bioRxiv - Plant Biology 2021Quote: ... ground into powder and dissolved in 200 μL 1 × Cell Culture Lysis Reagent (Promega), followed by centrifugation for 15 min at 4 °C ...