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Citations for Promega :
3001 - 3050 of 5099 citations for 7 hydroxy 1H 1 2 4 triazolo 1 5 a pyrimidin 5 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... the proteins were digested with 1 µg of trypsin/Lys-C mix (Promega) for 16 h at 37 °C ...
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bioRxiv - Plant Biology 2023Quote: ... samples were digested with Sequencing Grade Modified trypsin (0.5 µg µL-1; Promega) overnight at 37 °C ...
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bioRxiv - Plant Biology 2023Quote: ... followed by Western blotting using Anti-HaloTag monoclonal Ab (1:2000) (Promega, G9211) and THE GST monoclonal Ab (1:20000 ...
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bioRxiv - Cell Biology 2023Quote: ... 30 μL of CellTiter-Glo reagent solution (Promega, diluted 1:6 in water) was added to each well after 24 h and 72 h of compound treatment ...
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bioRxiv - Immunology 2023Quote: ... and 1 mg of RNA was reversed into cDNA by reverse transcriptase ( Promega), which reverse transcribed the Moloney mouse leukemia virus ...
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bioRxiv - Molecular Biology 2023Quote: ... Digestion was performed at 37 °C overnight with 1 µg of trypsin (Promega). After digestion ...
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bioRxiv - Neuroscience 2023Quote: ... and dyes used were anti-Halo-Tag (Promega, cat. G928A, 1:200 dilution), FluoTag-X2-anti-PSD95-Sulfo-Cy5 (28)(NanoTag Biotechnologies GmbH ...
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bioRxiv - Molecular Biology 2023Quote: ... and 1 μl of premix [100 μM amino acid mixture minus methionine (Promega), 100 μM amino acid mixture minus leucine (Promega) ...
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bioRxiv - Developmental Biology 2024Quote: ... We used the following primary antibodies: mouse anti-LacZ (1:500 dilution, Promega) rabbit polyclonal anti- cleaved Caspase 3 (1:200 dilution ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples incubated with Trypsin / Lys-C mix (1:70 protease:substrate ratio; V5072, Promega) for 4 hours ...
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bioRxiv - Immunology 2024Quote: ... Reactions were performed with the GoTaq Probe 1-Step RT-qPCR System (Promega) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... horseradish peroxidase-conjugated anti-mouse IgG or anti-rabbit IgG (1:25000; Promega) was reacted for 3 h ...
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bioRxiv - Molecular Biology 2024Quote: ... Trypsin 1:50 (w/w) (Gold Mass Spectrometry grade, Promega, Madison, WI, USA) was added to each sample for proteolytic digestion and left incubating overnight at 37°C ...
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bioRxiv - Biochemistry 2024Quote: ... and digested overnight with a 1:50 ratio of protein:trypsin (Promega, sequencing grade). Detergents were removed through organic-aqueous phase extraction with ethyl acetate ...
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bioRxiv - Molecular Biology 2021Quote: ... 7 pmol biotinylated RNAs were incubated with streptavidin beads in the binding buffer supplemented with 80 U RNasin (Promega, Germany, Cat. No. N2511) and 50 µg yeast tRNA (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... 100 uL of the diluted lysate was added to each well along with an equal volume of Caspase Glo 3/7 assay reagent (Promega, Madison, Wisconsin, USA), according to the manufacturer’s directions ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... membrane integrity (CytoTox-OneTM homogeneous membrane integrity assay kit: lactate dehydrogenase (LDH release) and caspase activity/ apoptosis (Caspase-Glo® 3/7 assay system kit) (all from Promega, WI, USA), as described previously17 (see Supplementary data for detailed description).
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bioRxiv - Cell Biology 2022Quote: ... β-arrestin 2 fused at the N-terminus to LgBiT (LgBiT-β-arrestin 2; Promega, plasmid no. CS1603B118) was chosen as it has previously been used successfully with other class B GPCRs ...
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bioRxiv - Plant Biology 2021Quote: ... the protein suspensions were digested with 4 μg trypsin (Promega) in 40 μl TEAB buffer overnight at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... 25 μL of 4 mM luciferin (Assay Reagent, Promega, E1291) supplemented with isoproterenol at a final concentration of 200 nM was added ...
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bioRxiv - Microbiology 2023Quote: ... the protein suspension was digested with 4 μg trypsin (Promega) in 40 μL NH4HCO3 (50 mM ...
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bioRxiv - Neuroscience 2023Quote: ... After embedding in 4% low-melting point agarose (Promega, #V2111) in PBS ...
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bioRxiv - Cell Biology 2023Quote: ... and then 4 hours in mass-spectrometry-grade trypsin (Promega). The missed cleavage rate for samples was an average of 3.9%-21.9% ...
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bioRxiv - Microbiology 2022Quote: ... medium was changed and cellular expression of luciferase reporter indicating viral infection was determined using ONE-Glo™ Luciferase Assay System (Promega) in 48 hr after infection ...
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bioRxiv - Molecular Biology 2021Quote: ... 25 ul of this mixture was added 25 ul of the ONE-Glo™ EX reagent following 25 ul of NanoDLR Stop & Glo reagent (Promega). All mixtures were incubated in opaque white 96-well plates on a rocking shaker in the dark for 5 min before quantifying the luminescence.
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bioRxiv - Physiology 2019Quote: ... Samples were mixed with the same amount of substrate provided by the One Glo Luciferase Assay System (Promega, Mannheim, Germany, #E6110) immediately before signal detection ...
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bioRxiv - Cancer Biology 2019Quote: ... Cell cytotoxicity in the drug-treated 2D cell cultures was assessed using the CellTiter96® AQueous One Solution Cell Proliferation Assay (Promega). Briefly ...
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bioRxiv - Microbiology 2019Quote: ... the cell viability of each infected virus variants was measured using CellTiter 96 Aqueous One solution Cell Proliferation Assay (Promega, USA). The relative cell viability was calculated by normalizing the absorbance value of treated virus samples against untreated virus samples ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The cell viability was measured after 24 hours by adding 20 μl of CellTiter 96® AQueous One Solution Reagent (Promega) directly to the wells ...
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bioRxiv - Genomics 2020Quote: ... TRs were amplified using the forward and reverse primers plus an M13 primer with one of the four fluorophores with GoTaq polymerase (Promega #PRM7123) using PCR program ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were incubated with nanoparticles encapsulating Fluc mRNA and analyzed for cell viability and luciferase activity with the ONE-Glo™+Tox luciferase reporter and cell viability assay kit (Promega) using a multimode microplate reader.
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bioRxiv - Neuroscience 2020Quote: ... CellTiter 96® Aqueous One Solution cell proliferation assay kit and ApoTox-Glo™ Triplex Assay kit was obtained from Promega, Australia ...
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bioRxiv - Cell Biology 2021Quote: ... or without (150 bp) one putative ETV2 binding site (Wei et al, 2010) were cloned into pGL3_Basic vector (Cat# E1751, Promega, Madison, WI). Primers Forward ...
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bioRxiv - Cancer Biology 2019Quote: Proliferation of cancer cells isolated from MMTV-PyMT;Ccr2+/+ and MMTV-PyMT;Ccr2−/− mice was measured using the CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS; Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Cell viability was measured 48h later (or otherwise indicated) using CellTiter 96® AQueous One Solution Cell Proliferation Assay (G3582, Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: Human SCLC cell lines plated in a 96-well microplate at a concentration of 5×103 cells/well were used to determine cell viability after 72h of incubation using CellTiter 96® AQueous One System (Promega). Epoch microplate spectrophotometer (Biotek ...
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bioRxiv - Cell Biology 2020Quote: ... and results were normalized to living cell numbers as evaluated with the CellTiter 96® AQueous One Solution Cell Proliferation Assay Kit (Promega) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... The virus-serum mix was subsequently used to infect A549-hACE2-TMPRSS2 cells for 18 hr at 37 Celsius before adding ONE-Glo reagent (Promega E6120) for measurement of luciferase signal (relative luminescence unit ...
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bioRxiv - Molecular Biology 2021Quote: ... and another 25 μL of cell lysate was mixed with 25 μL of ONE-Glo Luciferase Assay System (Promega, Cat.# E6130). The plate was placed on a shaker for 5 min in the dark ...
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bioRxiv - Bioengineering 2022Quote: ... The cell proliferation assay was conducted with CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) reagent (Promega, Cat.No. G3582) following the instruction ...
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bioRxiv - Neuroscience 2023Quote: Cytotoxicity was assessed by measuring the lactate dehydrogenase (LDH) release from cells with damaged membranes using the CytoTox-ONE Homogeneous Membrane Integrity assay (#7891, Promega, USA). For this ...
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bioRxiv - Microbiology 2023Quote: ... One aliquot was used for DNA extraction using the Promega Wizard® Genomic DNA Purification kit (Promega Corporation, Madison, WI, USA) as per the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... One µL of lysate was then added to make 20µL PCR reactions with GoTaq G2 Green Master Mix (Promega, Cat# M7823) and 1µM forward and reverse primers (Table 1) ...
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bioRxiv - Microbiology 2023Quote: ... and cellular expression of the luciferase reporter indicating viral infection was determined using the ONE-Glo™ Luciferase Assay System (Promega) 48 h after infection ...
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bioRxiv - Microbiology 2023Quote: ... the cleaned PCR product was eluted from the beads in 10 µL of buffer solution containing 10 mM Tris-HCl pH 8.0 with 50 mM NaCl and the DNA concentration was quantified on a Quantus Fluorometer using Quantifluor ONE dsDNA dye (Promega #E4891).
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bioRxiv - Bioengineering 2023Quote: ... low passage cells were plated at a density of 15,000 cells per well in a 96-well plate and transfected at > 80% confluence with all-in-one PiggyBac plasmids containing CasRx or DjCas13d using ViaFect™ Transfection Reagent (E4981, Promega) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... cells were transfected at > 80% confluence with all-in-one PiggyBac plasmids containing different Cas13d orthologs using FuGENE® HD Transfection Reagent (E2311, Promega) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... low passage cells were plated at a density of 15,000 cells per well in a 96-well plate and transfected at > 80% confluence with all-in-one PiggyBac plasmids containing CasRx or DjCas13d using FuGENE® HD Transfection Reagent (E2311, Promega) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... cells were transfected at > 80% confluence with all-in-one PiggyBac plasmids containing different Cas13d orthologs using FuGENE® HD Transfection Reagent (E2311, Promega) according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2023Quote: ... 10 μL of the dilution was mixed with 20 μL of passive buffer which was then mixed with 30 μL ONE-Glo™ EX Luciferase Assay Reagent (Promega) and incubated at room temperature for 10 min ...