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Citations for Promega :
3051 - 3100 of 5313 citations for 6 Bromo 3 3H imidazol 4 ylmethylene 1 3 dihydro indol 2 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2024Quote: ... The samples were diluted with 50 mM TEAB buffer to a final Urea concentration of 2 M before adding trypsin (Promega) in an enzyme:substrate ratio of 1:75 and incubating at 25 °C for 16 hours ...
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bioRxiv - Immunology 2024Quote: ... 48 hours later the media was removed and replaced with 25 µL of RMPI-1640 media supplemented with 2% low-IgG FBS (Promega). Antibodies were serially diluted 1:10 in RPMI 1640 media from a starting concentration of 30 µg/mL to a final concentration of 0.014 µg/mL and 25 µL of antibody dilution was added to the plate ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... cells were pre-incubated in Hanks’ balanced salt solution with increasing concentrations of compound for 60 minutes before stimulation with 10 nM CXCL12 and addition of 2 µM Renilla Luciferase substrate coelenterazine-h (Promega). After 20 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... dilute with 4 volumes of 20 mM Tris-HCl pH8 / 2 mM CaCl2 before overnight digestion at room temperature with 100 ng sequencing grade trypsin (Promega). Samples were centrifuged ...
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bioRxiv - Immunology 2022Quote: ... were sorted by FACS in 96-well V-bottom plate wells containing 2 μl of lysis buffer (Ultra-pure Water, 10% Triton X-100, RNasinPlus 40U/ul; Promega). After evaporation of the lysis buffer at 95°C for 3 min ...
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bioRxiv - Bioengineering 2022Quote: HEK293T cells with the RAI13103insC mutation were generated by introducing the plasmid expressing Cas9 and sgRNA with single-stranded oligodeoxynucleotides (Supplementary Sequence 2) by FuGENE HD (Promega). The Cas9-expression plasmid with the sgRNA sequence for generating the RAI13103insC mutation and the GFP/puromycin expression plasmid (70 ng of each plasmid ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were subjected to in gel tryptic digest overnight at 37 °C with approximately 2 µg trypsin per 100 µl gel volume (Trypsin Gold, mass spectrometry grade, Promega). Peptides were eluted twice with 100 mM NH4HCO3 followed by an additional extraction with 50 mM NH4HCO3 in 50% acetonitrile ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were diluted to a final concentration of 1e6 cells per mL in Expi293 expression medium plus 2% (v/v) Glosensor assay reagent (Promega) and intubated for 75 min at room temperature with gentle rotation ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were harvested 2 days hours following transfection and reporter assays were performed using the Dual-Luciferase Reporter Assay (Promega).
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bioRxiv - Microbiology 2023Quote: Cells infected with a luciferase-encoding virus were lysed 2 days after infection with a Bright-Glo Luciferase Assay System (E2620, Promega) and the luminescent signal was measured using a GloMax Explorer Multimode Microplate Reader (Promega).
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bioRxiv - Immunology 2023Quote: Expression vectors encoding Foxp3 and Ikzf1 and/or Ikzf1 mutants were transfected into HEK293T cells (2 × 105) with FuGENE HD (Promega). 48h after transfection ...
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bioRxiv - Developmental Biology 2023Quote: ... Nuclei were washed 3 times by resuspending in cold PBS containing 2% fraction V bovine serum albumin and 0.2 U / µl RNase inhibitor (Promega, N2615). For multiome assay ...
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bioRxiv - Biochemistry 2023Quote: ... cells were starved by 50 μL Hank’s balanced salt solution for 30 min and then incubated in 50 μL CO2-independent media containing 2% GloSensor cAMP Reagent (Promega) for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... Specific adapters 1 and 2 described in Table B were self-hybridized and then ligated to the ‘A’ tail ends by T4 ligase (Promega). After purification with AMPure XP purification kit (Agencourt) ...
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bioRxiv - Biochemistry 2023Quote: ... proteins were diluted with 50 mM NH4HCO3 to a final concentration of 2 M urea and digested with trypsin (Promega), at an enzyme-to-substrate ratio of 1:20 ...
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bioRxiv - Neuroscience 2023Quote: ... luminescence photons were collected by accumulating the image for 60 s in the presence of 2 mM D-luciferin (Promega), and the luminescence signal was measured from the same varicosities as the corresponding fluorescence image ...
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bioRxiv - Biochemistry 2023Quote: ... then washed and resuspended in 2 M urea and trypsinized overnight with 0.5 μg/μl sequencing grade trypsin (Promega, V5111). Tryptic peptides were eluted off with spin columns (BioRad ...
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bioRxiv - Cancer Biology 2023Quote: ... First-strand complementary DNA (cDNA) synthesis was performed using 2 μg of total RNA with M-MLV reverse transcriptase (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: The activity of EBV miRs-BART 7-3p and 9-3p was evaluated in Akata-EBV/Cas9 cells by luciferase reporter gene assay with constructs based on the psiCheck-2 backbone (Promega). 3’-UTR sequences with miRNA-binding sites (Supplementary Material ...
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bioRxiv - Molecular Biology 2023Quote: ... siRNAs were detected with a YFP long probe (primers listed in Supplemental Table 2) labeled with 32P-dCTP prepared according to the manual of the Prime-a-Gene Labeling System (Promega). The blot was hybridized overnight at 42 °C with the probe before being washed with 2xSSC ...
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bioRxiv - Microbiology 2023Quote: ... injecting 50 μl per well of coelenterazine substrate (Nanolight Technologies, 2 μg/ml) and analysing luminescence on a FLUOstar OPTIMA luminometer (Promega). Fold inductions were calculated by normalising to a mock-treated control.
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bioRxiv - Evolutionary Biology 2023Quote: ... reverse transcription was performed using Promega’s Go-Taq 2-Step system with oligo(dT) and randomized primers as per manufacturer’s instructions (Promega, Madison, WI).
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bioRxiv - Plant Biology 2023Quote: ... Purified DNA was run on 2% agarose gel and bands corresponding to ∼150 bp were cut and purified with a Gel Purification kit (Promega). Libraries were constructed using the Nugen Ovation Ultralow Library System V2 following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... gondii glyceraldehyde 3-phosphate dehydrogenase 2 (GAPDH2, ML5049/ML5680) or TgAPT1 (ML4097/ML4098) were used for subsequent PCR amplification with GoTaq polymerase (Promega) for twenty-five cycles as follows ...
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bioRxiv - Microbiology 2023Quote: Vero E6 cells were transfected with an GFP expression vector together with either a control expression vector (no spike) or a SARS-CoV-2 spike expression vector using Fugene (Promega). Two hours after transfection ...
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bioRxiv - Systems Biology 2023Quote: ... The panel of ssDNA-labelled antibodies (250 ng/ml for each antibody) was mixed with 2 U/µl RNAsin Plus (Promega) and 0.1% Triton X-100 in PBS:PFBB (1:1 ...
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bioRxiv - Biochemistry 2023Quote: ... supplemented with 4% FBS and 100 µL were seeded per well in 96-well plates at a density of 2×105 cells/mL in the presence or absence of 0.1 mM HaloTag NanoBRET™ ligand (Promega). The following morning ...
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bioRxiv - Immunology 2023Quote: ... cDNA was generated from 20-100 ng of RNA using the GoTaq 2-step RT-qPCR System (Promega, cat# A6110). qPCR was performed with SYBR Green on a StepOnePlus real-time PCR system (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2024Quote: ... The PCR fragment was directionally cloned downstream of the Renilla luciferase ORF in the psiCHECK-2 vector (Promega, Madison, US) using the Quick Ligase kit (M2200 ...
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bioRxiv - Cell Biology 2024Quote: ... 50 μL IVT reactions were carried out for 2 hours at 30°C using commercially available Nuclease-Treated Rabbit Reticulocyte Lysate (RRL, Promega) and reaction components were added in the following order ...
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bioRxiv - Plant Biology 2024Quote: ... The sample solutions were diluted to a final concentration of 2 M urea and 50 mM ammonium bicarbonate prior to the trypsin (Promega) digestion ...
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bioRxiv - Microbiology 2024Quote: In vitro toxicity was assessed in HeLa cells (ATCC® CCL-2) using the ApoTox-GloTM Triplex Assay (Promega, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: The 3’UTR of human Akt or Pin1 was amplified by PCR from fibroblast genomic DNA and cloned downstream of the Renilla luciferase gene in the psiCHECK-2 dual reporter construct (Promega) by XhoI (Akt ...
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bioRxiv - Developmental Biology 2021Quote: ... abdomens were washed with phosphate-buffered saline with 0.1% Tween (PBST) four times for 15 minutes before RNase (Promega, original concentration of 4 mg/mL) was added in a concentration of 10 μL/mL for 20 minutes ...
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bioRxiv - Immunology 2021Quote: ... then combined in a 1:1 ratio with Nano-Glo® Luciferase solution (Promega, Ref N1150). A Promega GloMax plate reader was used to measure luminescence.
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bioRxiv - Cell Biology 2022Quote: ... the samples were mixed in a 1:1 (v/v) ratio with luciferase assay reagent (Promega) in a 96-well white opaque plate and left at room temperature for 2 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... 60 μL of each sample was mixed 1:1 with Steady-Glo Luciferase system (Promega #E2510) following the manufacturer’s protocols ...
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bioRxiv - Biochemistry 2020Quote: ... The attached cells were lysed in 20 μl of 1× passive lysis buffer (1× PLB, Promega) at room temperature (RT ...
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bioRxiv - Cell Biology 2022Quote: ... the samples were mixed in a 1:1 (v/v) ratio with nucleotide detection reagent (Promega) in a 96-well white opaque plate and left at room temperature in the dark for another 60 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... the samples were mixed in a 1:1 (v/v) ratio with luciferase assay reagent (Promega) in a 96-well white opaque plate and left at room temperature for 2 minutes ...
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bioRxiv - Systems Biology 2019Quote: ... Single cells were collected in 1 μL of cell lysis buffer (1 U RNasein plus [Promega, Madison ...
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bioRxiv - Cell Biology 2020Quote: ... 1000 ng of donor and gRNA plasmids in 1:1 ratio was used using ViaFect (Promega) transfection reagent as per manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2022Quote: ... rabbit anti-GFP 1:2000 (MBL, RRID: AB_591819) rabbit anti-Halo 1:1000 (Promega, RRID: AB_713650), rat anti-HA 1:500 (Sigma-Aldrich ...
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bioRxiv - Biophysics 2022Quote: ... mixed 1 to 1 (v / v) with 10 μg / ml trypsin (Sequencing Grade Modified Trypsin, Promega) in 50 mM ammonium bicarbonate and incubated for 2 hours at 60 °C for enzymatic hydrolysis ...
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bioRxiv - Immunology 2024Quote: ... organoids were collected and lysed in 100 µl of a 1:1 dilution containing CellTiterGlo (Promega) and PBS ...
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bioRxiv - Immunology 2022Quote: Caspase-1 activity was quantified using a Caspase-Glo 1 Inflammasome Assay kit (Promega, Madison, WI). THP-1 cells were treated and harvested as described above ...
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bioRxiv - Immunology 2023Quote: ... then combined in a 1:1 ratio with Nano-Glo® Luciferase solution (Promega, Ref N1150). A Promega GloMax plate reader was used to measure luminescence ...
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bioRxiv - Cell Biology 2023Quote: ... Top half was incubated in anti-Nup107 (1:1000; ab236634) and HaloTag (1:500; Promega G9211). Bottom half was incubated in anti-GAPDH (1:1000 ...
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bioRxiv - Cancer Biology 2023Quote: ... organoids were stained using Hoechst 33342 (1 μg/mL) and CellTox Green (Promega, 1/20 000) dyes for 8 h prior to imaging at Opera Phenix (Perkin Elmer ...
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bioRxiv - Genetics 2023Quote: ... and for 1 hour at room temperature in secondary antibody (1:4,000 anti-Mouse-HRP, Promega). In order to detect the endogenous Ptiwi09 protein ...