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Citations for Promega :
251 - 279 of 279 citations for Hantaan Virus Nucleoprotein E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Total 500 ng RNA was used to synthesize cDNA by using Moloney-murine leukemia virus (M-MLV) reverse transcriptase (Promega, Madison, WI). Diluted cDNA was used in each quantitative reverse transcription-PCR (qRT-PCR) ...
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bioRxiv - Plant Biology 2022Quote: ... cDNAs were synthesized from 1.5 μg of total RNA with oligo(dT) primers using Moloney Murine Leukemia Virus Reverse Transcriptase MMLV-RT according to the manufacturer’s instructions (Promega, Madison, WI, USA). RT-qPCR was carried out in a Chromo4 system (Bio-Rad ...
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Mucociliary Clearance Augmenting Drugs Block SARS-Cov-2 Replication in Human Airway Epithelial CellsbioRxiv - Microbiology 2023Quote: ... The plates were incubated at 37°C under 5% CO2 for 144 hour and virus cytopathic effect was measured using the CellTiter-Glo® 2.0 Cell Viability Assay (Promega, Cat. # G9241) following the manufacturer instructions ...
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bioRxiv - Microbiology 2023Quote: ... the amount of input virus was normalized to the HiBiT value measured by Nano Glo HiBiT lytic detection system (Promega, Cat# N3040)] ...
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bioRxiv - Microbiology 2023Quote: ... Samples where a 1 log decrease in RLU compared to the no-serum virus-only control was observed were then selected for cytotoxicity assay using Cell Titre Glo kit (Promega, Southampton, UK) and light microscopy to verify viability of cells prior to undertaking PVNA (data not shown) ...
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bioRxiv - Microbiology 2024Quote: ... followed by reverse transcription to complementary DNA (cDNA) using 30 enzyme units(U) of the Moloney Murine Leukemia Virus reverse transcriptase (MMLV-RT) (Promega, WI, USA). The extension products were then analysed using the ABI3730xl DNA Analyzer (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2022Quote: ... coli isolates from Vietnam was extracted using the Wizard Genomic DNA purification kit (Promega, Madison, WI, USA) following the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Transformation of successful ligation product was performed in Escherichia coli Trans5α competent cells (Promega, Madison WI, USA). Plasmids with insertions were extracted and purified ...
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bioRxiv - Microbiology 2020Quote: ... coli isolates was extracted using Wizard® Plus SV Mini preps plasmid DNA Purification kit (Promega, USA) according to manufacturer’s instruction and was electrophoresed in 0.8% agarose gel (Rakhi et al. ...
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bioRxiv - Plant Biology 2022Quote: ... plasmids were purified from overnight-cultured competent Escherichia coli (DH5α) using a PureYield Plasmid Midiprep System (Promega) and the phenol-chloroform method.
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bioRxiv - Plant Biology 2020Quote: ... First strand cDNA synthesis was carried out using 2 ug of total RNA and reverse transcription was performed using an oligo (dT) 15 primer and Moloney murine leukemia virus (MMLV) reverse transcriptase (Promega, Madison, WI, USA). qPCR was carried out using SYBR GREEN PCR Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... oligo deoxythymidylic acid (oligo-dT) primed cDNA was synthesized from 500 ng of total RNA using Murine Moloney Leukaemia Virus Reverse Transcriptase (Promega, Madison, WI, USA). RT-qPCR was performed on the ViiA 7 Real-Time PCR System (Applied Biosystems/Thermofisher ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... PHH were infected with HepAD38-derived GTD virus at 500 viral genome equivalents per cell in maintenance medium supplemented with 4 % PEG 8000 (Promega, Madison, WI; V3011). For patient sera infections ...
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bioRxiv - Pathology 2020Quote: ... cDNA synthesis from the mRNA was performed using a Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) kit (Promega, Madison, Wisconsin, USA) in a Mastercycler® Gradient (Eppendorf ...
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bioRxiv - Genomics 2020Quote: ... Five micrograms of RNA was reverse transcribed into cDNA using Moloney-murine leukemia virus reverse transcriptase and random primers (Promega, Madison, Wisconsin, USA) in a 20 μl reaction volume ...
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bioRxiv - Plant Biology 2022Quote: ... Subsequently 2 μg of RNA were used to perform reverse transcription in the presence of Moloney murine leukemia virus reverse transcriptase (Promega, Madison, WI, USA) after annealing with an anchored oligo(dT)18 primer as described by (Wirth et al. ...
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bioRxiv - Biochemistry 2024Quote: ... was synthesised from 2 µg of total RNA using the Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT; Promega, Madison, WI, USA) using random primers and oligo-dT primers (3:1 mol ...
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bioRxiv - Biochemistry 2020Quote: ... or ∼30 ng (Proevolidine and Proxanthoxycyclin E gDNA) of the A-tailed PCR product were ligated into 50 ng of the pGEM-T Easy vector (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... PLAT-E cells were transfected with the pMYs STEAP1-mBBζ CAR retroviral construct using the FuGENE HD transfection reagent (Promega). 48 and 72 hours after transfection ...
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bioRxiv - Neuroscience 2021Quote: ... The amplified coding sequence was then subcloned into the pGHJ vector after double digestion with SmaI e EcoRI restriction enzymes (Promega). hTGR5 gene was in pCMV6-Entry (GPBAR1 Human cDNA ORF Clone ...
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bioRxiv - Cancer Biology 2021Quote: ... and MCF-7/CA-IX cell lines by standard clonogenic stable cell construction procedures using Fugene HD (Promega, E 2311). The U2-OS and HEK-293 cells transfected with empty pCMV6 (PS10001 ...
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bioRxiv - Genetics 2022Quote: ... The amplification was performed in 20 μL of reaction (2XPCR FailSafeTM PreMix E from Biosearch Technologies, 1.25 U of GoTaq DNA Polymerase (Promega), < 1μg DNA template and 0.5 μM of each specific primers ...
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bioRxiv - Immunology 2023Quote: ... Retrovirus was generated by transfecting the Plat-E packaging cell line with 10 µg plasmid DNA and 30 µl FuGene HD transfection reagent (Promega). Viral supernatants were collected from transfected Plat-E cells at 40 ...
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bioRxiv - Cancer Biology 2021Quote: ... For these experiments pGL2M4-luc reporter plasmid37 (containing four CACGTG binding sites, a canonical E-box) and pRL Renilla Luciferase Control Reporter Vectors (CMV, Promega E2231) were used ...
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bioRxiv - Cancer Biology 2021Quote: The anal tissue was scraped from H&E stained slides using a sterile blade.[13] The deparaffinization and genome DNA extraction from H&E stained anal tissues was performed according to manufacturer’s instructions using ReliaPrep FFPE gDNA MiniPrep System (Promega, Madison, WI).
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bioRxiv - Microbiology 2019Quote: ... was used as a template for the amplification of the Δ3(E)-desaturase gene FJ176923 with Pwo-polymerase (peqlab) and ligated into vector pGEM®-T (Promega). After restriction with EcoRI and NotI ...
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bioRxiv - Microbiology 2023Quote: The viral RNA derived from the lung and nasal turbinate samples was quantified using a protocol for quantifying the SARS-CoV-2 sub-genomic E gene RNA (sgE)29 using the GoTaq® Probe 1-Step RT-qPCR System (Promega).
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bioRxiv - Synthetic Biology 2021Quote: All experiments were performed using Caulobacter crescentus NA1000 (also known as CB15N) and Escherichia coli DH5α and BL21 purchased from Promega. C ...
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bioRxiv - Biophysics 2019Quote: ... coli HB101 cells and isolated in quantity by using a Promega Wizard Plus Megaprep purification kit (Promega Corp., Madison, WI).