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Citations for Promega :
151 - 200 of 279 citations for Hantaan Virus Nucleoprotein E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Reverse transcription was then performed with 50 U of Avian Myeloblastosis Virus reverse transcriptase (Promega Corporation) for 20 min at 37 °C ...
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bioRxiv - Cancer Biology 2023Quote: Reverse transcription was performed using the Moloney Murine Leukemia Virus Reverse Transcriptase (Promega, Madison, WI, USA), 1000 ng of total RNA and random hexamers ...
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bioRxiv - Immunology 2023Quote: ... Pseudotyped virus were prepared by transfection of HEK293T cells using the Fugene HD transfection system (Promega), as previously described(42) ...
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bioRxiv - Microbiology 2020Quote: ... coli strains was prepared using the Wizard Genomic DNA Purification kit (Promega) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... coli using a Wizard® Plus SV Minipreps DNA Purification System (Promega). C ...
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Identification and biochemical characterization of a novel eukaryotic-like Ser/Thr kinase in E. colibioRxiv - Microbiology 2020Quote: ... coli REL606 was isolated using a Wizard genomic DNA purification kit (Promega) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... pSY5M-Pak4cat were introduced into Escherichia coli strain BL21(DE3) pLysS (Promega), and the fusion proteins were induced by incubation with 0.5 mM isopropyl-b-D-thiogalactoside (IPTG ...
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bioRxiv - Microbiology 2023Quote: ... coli strain S17-1λpir and confirmed by colony PCR (GoTaq polymerase, Promega) and Sanger sequencing (Microsynth ...
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bioRxiv - Cell Biology 2020Quote: 1 µg of total RNAs were reverse transcribed with Moloney murine leukemia virus Reverse Transcriptase (M170B, Promega). 100 ng cDNA and FastStart Universal SYBR Green Master (04913850001 ...
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bioRxiv - Immunology 2021Quote: ... and reversed transcribed with random hexamers (IDT) using Moloney murine leukemia virus (M-MLV) reverse transcriptase (Promega) according to the manufacturers’ instructions ...
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bioRxiv - Microbiology 2021Quote: ... Virus -specific PCR products were cloned into pGEM-T Easy Vector (A1360, Promega Co., Madison, WI, USA) and used to transform E ...
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bioRxiv - Immunology 2021Quote: ... cDNA synthesis was performed using Moloney Murine Leukemia Virus Reverse Transcriptase (MML-V)(Promega Corporation, Madison, WI). ViiA-7 instrument (Life Technologies ...
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bioRxiv - Genomics 2022Quote: ... Reverse transcription was carried out using moloney-murine leukemia virus (M-MLV) reverse transcriptase kit (Promega, USA). Reverse transcription was completed in a 30 µL final volume reaction containing 400 U M-MLV and 1 mM dNTP mix (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... and 0.25 μg vesicular stomatitis virus G glycoprotein expression plasmids using the Fugene 6 transfection reagent (Promega) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2023Quote: ... and TCID50 equivalents determined using a SARS-CoV-2 E gene detection kit (Promega) based upon the Berlin primer/ probe set (70).
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bioRxiv - Developmental Biology 2020Quote: ... and transformed into competent JM-109 Escherichia coli cells (Promega, Madison, WI, USA). Colony-PCRs were conducted by using primers recommended by the kit at 94°C for 3 minutes ...
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bioRxiv - Microbiology 2020Quote: ... coli cultures using a Wizard® Plus SV Minipreps DNA Purification System (Promega). The pLZ12Km2-P23R:TA plasmid was digested with 20 U NotI enzyme and further treated with 5 U shrimp alkaline phosphatase ...
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bioRxiv - Plant Biology 2020Quote: ... and cDNA synthesized using Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) and universal Random hexamers (Promega). qRT-PCR was performed with 2x PowerUP™ SYBR™Green Master Mix in the QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Microbiology 2020Quote: ... Reverse transcription (RT) of different HCV genomic regions was performed using avian myeloblastosis virus (AMV) reverse transcriptase (Promega), and subsequent PCR amplification was carried out using AccuScript (Agilent Technologies) ...
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bioRxiv - Cell Biology 2021Quote: ... The reverse transcriptase enzyme M-MLV RT (H-) (Moloney Murine Leukemia Virus Reverse Transcriptase RNase H minus, Promega) was then used to transcribe 1 µg of total RNA to cDNA using Oligo(dT)15 primers (Promega) ...
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bioRxiv - Microbiology 2022Quote: ... virus was removed and cells were transfected with 2 µg of plasmid DNA pUCSP-A24Rcd using FuGeneHD (Promega) following manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2022Quote: ... A total of 1.5 μg RNA was reversely transcribed using Moloney murine leukemia virus reverse transcriptase (M1701, Promega) for 90 min at 37°C ...
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bioRxiv - Cancer Biology 2019Quote: ... Complementary DNA (cDNA) was synthesized using M-MLV (Moloney murine leukemia virus) reverse transcriptase (Promega, Madison, WI, USA), and amplified using SYBR Green qRT-PCR SuperMix-UDG reagents (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... virus-containing media was removed and replaced with 35 μl of 1x Passive lysis buffer (Promega; Ref# E194A). Plates underwent three freeze-thaw cycles consisting of freezing on dry-ice for 15 minutes followed by thawing at 37°C for 15 minutes ...
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bioRxiv - Microbiology 2021Quote: ... virus inoculum was removed and cells were then transfected with 2 µg of plasmid DNA using FuGeneHD (Promega) according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2020Quote: ... Isolated RNA (1 μg) was used to synthesize cDNAs using Murine Moloney Leukemia Virus reverse transcriptase (MMLV, Promega). Subsequently ...
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bioRxiv - Microbiology 2022Quote: ... The reverse transcription (RT) mix contained 200 units of recombinant Moloney Murine Leukemia Virus (MMLV) reverse transcriptase (Promega), 20 units of RNAsin (Promega) ...
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bioRxiv - Microbiology 2022Quote: ... reverse transcription was performed with 0.5 μl (corresponding to 5 U) of Avian Myeloblastosis Virus Reverse Transcriptase (Promega), 0.1 μl dNTP mix (10 mM) ...
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bioRxiv - Immunology 2023Quote: ... 2 μg RNA was used to synthesize first-strand cDNA using Moloney mouse leukemia virus (M-MLV) (Promega). The amplification of specific PCR products was detected using SYBR Premix Ex TaqTM II (Vazyme) ...
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bioRxiv - Cell Biology 2023Quote: ... A total of 1.5 µg RNA was reversely transcribed using 0.5 μl Moloney murine leukemia virus reverse transcriptase (M1701, Promega), 4 µl RT buffer 5x (Promega) ...
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bioRxiv - Microbiology 2024Quote: ... The cells were collected at 48 h post-infection and virus infectivity were measured by luciferase assay (Promega).
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bioRxiv - Microbiology 2023Quote: ... 800 ng pNL4.3.Luc.R-.E– (NIH Reagents program) and 200 ng pRL Renilla (Promega E2231) as control for nucleofection efficiency ...
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bioRxiv - Plant Biology 2020Quote: ... DNA-free RNA (500 µg) was used for reverse transcription using the Moloney Murine Leukemia Virus Reverse transcriptase (Promega, Madison ...
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bioRxiv - Microbiology 2020Quote: ... Two hundred nanograms of purified RNA were reverse-transcribed using Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV RT, Promega). RT was primed with oligo dT(15 ...
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bioRxiv - Microbiology 2021Quote: ... The cDNA was synthesized using 5μg of total RNA at 42 °C for 2 hours using avian myeloblastosis virus (AMV) reverse transcriptase (Promega). A 20 µL reaction containing 10 ng or 10 pg (for 16S rRNA ...
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bioRxiv - Cancer Biology 2020Quote: ... according to the manufacturer’s protocol and reverse transcribed with the Moloney Murine Leukemia Virus reverse transcriptase (M-MLVRT) (Promega) as follows ...
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bioRxiv - Microbiology 2022Quote: In vitro cytotoxicity of SMKIs on mock and/or virus infected A549 cells was determined using CellTiter-Glo 2.0 (CTG) Cell Viability Assay (Promega) according to manufacturer protocols.
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bioRxiv - Microbiology 2022Quote: Cytotoxicity of SMKIs on mock and/or virus-infected PCLS was determined using the LDH-Glo Cytotoxicity Assay (Promega) according to manufacturer’s protocols ...
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bioRxiv - Microbiology 2022Quote: ... for 1 h at 37 °C and cDNA was then synthesized using a Moloney murine leukemia virus (MMLV) reverse transcription kit with random primers following the manufactureŕs protocol (Promega). To check for the presence of remaining DNA ...
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bioRxiv - Cell Biology 2020Quote: ... Isolated RNA (500ng) was used for cDNA synthesis with mouse Maloney leukemia virus reverse transcriptase (Promega, Madison, WI, USA). SYBR Green RT-qPCR gene expression assays were performed in triplicate in an ABI StepOne Plus thermocycler using appropriate endogenous controls (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... virus was harvested and infectivity assayed by serial titration on HeLa TZM-bl reporter cells using Bright-Glo (Promega).
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bioRxiv - Microbiology 2022Quote: ... First-strand cDNA generation from total RNA was generated using Moloney murine leukemia virus (M-MLV) reverse transcriptase (Promega). Relative mRNA quantitation was done using the SYBR green quantitative real-time PCR (qRT-PCR ...
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bioRxiv - Pathology 2021Quote: ... Cytopathic effects of the virus were measured after 3 days using the CellTiter-Glo luminescent cell viability assay (Promega), according to the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The viability of virus- and mock-infected cells were measured using Cell Titer Glow assay (CTG; Promega, Madison, USA). The luminescence was read with a PHERAstar FS plate reader (BMG Labtech ...
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bioRxiv - Neuroscience 2021Quote: ... The constructs (1.2 µg) were cotransfected with vesicular stomatitis virus G (600 ng) and pSPAX2 (800 ng) using FuGENE 6 (Promega). The lentiviral supernatants were collected 2 days after transfection ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and virus entry ability in the selected organs was evaluated by luciferase activity using the luciferase assay system (Promega) and a microplate luminometer ...
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bioRxiv - Microbiology 2021Quote: ... followed by a reverse transcriptase (RT)-step with Moloney Murine Leukemia Virus RT (MMLV-RT, Promega, Madison, WI, USA) in the presence of RNase inhibitor (RNAsin plus ...
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bioRxiv - Microbiology 2021Quote: ... the cells were lysed with a 1:1 ratio of virus to Bright-Glo luciferase assay media (Promega, #E2610) and the contents were analyzed on a luminometer (LUMISTAR Omega ...
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bioRxiv - Immunology 2021Quote: ... Reverse transcription was performed with Moloney murine leukemia virus reverse transcriptase (MMLV-RT) according to the manufacturer’s protocol (Promega) using 200 ng of total RNA ...
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bioRxiv - Microbiology 2022Quote: ... Virus was incubated with cells for 24 hours and cells were subsequently lysed with Renilla Luciferase Assay System (Promega) according to manufacturer instructions ...