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Citations for Promega :
251 - 300 of 1032 citations for Adenovirus Type 5 Particles CMV β galatosidase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... using the luminescence β-glo assay as recommended by the manufacturer (Promega France ...
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bioRxiv - Microbiology 2020Quote: ... viral particles were purified by cesium chloride gradient centrifugation 82 and DNA extracted with Wizard Prep Columns (Promega, Corp.). Viral metagenome short-read libraries were prepared using the NexteraXT kit (Illumina ...
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bioRxiv - Microbiology 2019Quote: ... 5 μl of 5× Gotaq buffer (Promega M792A), 1μl of 10μM forward primer ...
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bioRxiv - Cell Biology 2022Quote: ... was generated by PCR-cloning the amplification of HaloTag coding sequence (primers HaloTag-For and HaloTag-Rev detailed below (IDTDNA, Coralville, IA)) from pFN21K HaloTag® CMV Flexi® Vector (Promega, Madison, WI), followed by ligation in place of the mApple sequence in the mApple-CD36-C-10 vector (plasmid # 54874 ...
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bioRxiv - Neuroscience 2020Quote: ... The cells were co-transfected with 1 μg of the experimental firefly luciferase construct and 0.2 μg internal pRL control plasmid expressing Renilla luciferase driven by the CMV promoter (Promega, Madison, WI, USA; Cat# E2261). 24 hours after transfection ...
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bioRxiv - Biophysics 2023Quote: ... was generated by PCR-cloning the HaloTag coding sequence (primers details in Table S1, Integrated DNA Technologies) from pFN21K HaloTag® CMV Flexi® Vector (Promega, Madison, WI), followed by ligation in place of the mApple sequence in the mApple-CD36-C-10 vector (Addgene ...
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bioRxiv - Cell Biology 2023Quote: Plasmids containing a promoter with binding sites for specific transcription factors to be measured driving firefly luciferase expression were co-transfected with a renilla luciferase reporter plasmid with a CMV promoter (Promega Corp, Madison, WI, USA) using TransIT reagent (0.25 µl/well ...
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bioRxiv - Cell Biology 2023Quote: ... wild-type and mutant tepsin-myc constructs were transfected using Fugene 4K (Promega) at 1.5:1 Fugene:DNA ratio following manufacturer protocol ...
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bioRxiv - Bioengineering 2021Quote: ... β-galactosidase activity was measured using a Beta-Glo Assay System (E4720, Promega). The Npas2-LacZ expression data were uploaded to an online data analysis tool (CDD Vault ...
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bioRxiv - Developmental Biology 2020Quote: ... The primary antibodies that were used are mouse β-Gal (1:250; Promega), mouse anti-Cora C615.16 (1:400 ...
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bioRxiv - Cell Biology 2022Quote: ... β and γ DNA was next PCR amplified with the Q5 polymerase (Promega) according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... The following antibodies were used for immuohistochemistry: mouse anti-β-gal (Z3781, Promega), chicken anti-NeuN (266006 ...
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bioRxiv - Cancer Biology 2019Quote: Replication-deficient retro- and lentiviral particles were produced by transient transfection of 293T cells using FuGENE® 6 reagent (Promega). In brief ...
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bioRxiv - Microbiology 2019Quote: HIV-1 and lentiviral particles were produced by transfection of HEK293T cells in T150 flasks using Fugene 6 transfection reagent (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... The first round of selection was performed manually using 300 nM of biotinylated K29-linked diubiquitin immobilized onto 250 µL of Streptavidin Paramagnetic Particles (Promega). Following washing with Selection Buffer (SB ...
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bioRxiv - Genomics 2020Quote: ... Viral RNA was extracted using the silica guanidinium isothiocyanate binding method (12) adapted for the ThermoFisher Kingfisher using paramagnetic silica particles (Magnesil, Promega).
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bioRxiv - Molecular Biology 2019Quote: ... Virus-like particles (VLPs) containing the GFP control or targeting sgRNAs were generated by transfection of HEK293 cells with FuGene (Promega). VLPs were collected from culture supernatant ...
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bioRxiv - Neuroscience 2019Quote: ... genomic DNA from puromycin selected cells and wild-type HEK293 cells was extracted (Promega Wizard Genomic DNA Purification Kit #A1120 ...
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bioRxiv - Genetics 2022Quote: Type-1 quantitative bioluminescence resonance energy transfer (qBRET) experiments utilised the NanoBRET platform (Promega) and followed the general type-1 qBRET protocol reported by Felce et al.25 The assay was performed in HEK293 cells transiently expressing POPDC1 and POPDC2 constructs which possessed a C-terminal NanoLuc luciferase or HaloTag domains ...
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bioRxiv - Cell Biology 2020Quote: ... We used the following primary antibodies: mouse anti-β-galactosidase (Promega, 1:1,000 dilution), rabbit anti-GFP (MBL,1:1,000 dilution) ...
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bioRxiv - Microbiology 2019Quote: Virus infectivity and neutralization was measured using a β-galactosidase-based luciferase assay (Promega) with TZM-bl target cells ...
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bioRxiv - Developmental Biology 2021Quote: ... and Dad13 constructs were detected with using mouse-anti-β-galactosidase (1:1000, Promega) and rabbit-anti-β-galactosidase (1:1000 ...
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bioRxiv - Developmental Biology 2023Quote: ... on embryos/larvae using mouse anti-β-gal (Promega catalog number Z3781, 1:1000) and rabbit anti-mCherry (BioVision ...
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bioRxiv - Developmental Biology 2023Quote: ... rabbit and mouse anti-β-Gal (MP Biomedics #559761 and Promega #Z378A, 1:1000), rabbit and mouse anti-pH3 (Merck Millipore #06-570 and Cell signal technology #9796 ...
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bioRxiv - Microbiology 2023Quote: 1 ml of recombinant His-MBP-AlpA and His-MBP at a concentration of 10 μM was incubated with 100 μl MagneHis™ Ni Particles (Promega) equilibrated with pulldown-buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Bioengineering 2021Quote: ... Cell lysates were pre-cleared of any non-specific streptavidin-binding proteins by incubating with 60 µL of MagneSphere paramagnetic particles (Promega, Madison, WI) for 30 min with rotation ...
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bioRxiv - Microbiology 2023Quote: ... The amount of produced pseudovirus particles was quantified by the HiBiT assay using Nano Glo HiBiT lytic detection system (Promega, Cat# N3040) as previously described14 ...
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bioRxiv - Immunology 2020Quote: ... and firefly luciferase and β-galactosidase activity were measured using the Luciferase Assay System (Promega) and Galacto-Ligh System (Applied Biosystems) ...
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bioRxiv - Neuroscience 2021Quote: ... Neurite outgrowth was visualized by immunostaining with mouse anti-β III tubulin (1:1000; Promega) followed by incubation with Alexa 594-conjugated anti-mouse secondary antibodies (Invitrogen).
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bioRxiv - Cell Biology 2020Quote: ... Luciferase and β-galactosidase activities were measured according to the manufacturer’s instruction (Promega, Madison, WI).
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bioRxiv - Molecular Biology 2023Quote: ... Bacterial lysates expressing fused GST-CuLCrV / SiGMV / TYLCV CP proteins and GST control were incubated with MagneGST□ particles (cat#V8600, MagneGST protein purification system, Promega, Madison, WI, USA) to immobilize the GST tagged proteins as per manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: CosM6 cells were transfected with ∼1 µg of wild type or mutant constructs using FuGENE6 (Promega) and were patched within 1-2 days after transfection ...
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bioRxiv - Synthetic Biology 2019Quote: The wild type and mutant promoter of miR-146a-5p were constructed into pGL3 vector (Promega). The pGL3-WT or pGL3-mut were co-transfected with pRL-TK using lipofectamine® 3000 ...
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bioRxiv - Biochemistry 2019Quote: Wild-type and mutant AP2ΔµC complexes were expressed in BL21 (DE3) pLysS strain (Promega, Madison, WI) by induction at 23 °C overnight ...
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bioRxiv - Cancer Biology 2021Quote: ... wild type or mutant 3′-UTR of DKK3 was cloned into the psicheck-2 vector (Promega). HCT116 cells were transfected with and wild-type or mutant 3′-UTR-luc by using Lipofectamine 3000 ...
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bioRxiv - Molecular Biology 2023Quote: ... The wild-type and mutants of MCHR1 and MCHR2 were constructed into the pcDNA6.0 vector (Promega) for functional assays.
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bioRxiv - Developmental Biology 2020Quote: ... with an added 5’-CACC-3’ at 5’-end and 5’-AAAC-3’ at 5’-end of the complementary strand were annealed in 10xT4 ligation buffer (Promega M1801) by continuous cooling from 95°C to 25°C ...
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bioRxiv - Cell Biology 2019Quote: ... These reporter vectors were transfected into HepG2 cells along with a β-galactosidase control plasmid (Promega) to normalize transfection efficiency ...
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bioRxiv - Microbiology 2023Quote: ... 2.5 mM β-mercaptoethanol and 0.5 mM each dNTP upon which reverse transcriptase (Promega; 40 units) was added ...
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bioRxiv - Microbiology 2020Quote: ... with m7G(5')ppp(5')G RNA Cap Structure Analog (ref. S1404L, Promega) as recommended by the manufacturer ...
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bioRxiv - Microbiology 2023Quote: ... 5’-CGCCTTCGTTACAAGCATCG-3’; antisense, 5’-AAGAGCAAACGCATCTCCGA-3’), GAPDH (sense, 5’-ACCCACTCCTCCACCTTTGAC-3’; antisense, 5’-CTGTTGCTGTAGCCAAATTCGT-3’) using Green GoTaq master mix (Promega, Madison, WI) with C1000 Touch Thermal Cycler (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: DNA was extracted from each cell type with the Wizard Genomic DNA Purification Kit (Promega, Southampton, UK) and send for STR profiling (Core Genomic Facility ...
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bioRxiv - Plant Biology 2020Quote: ... oryzae (Pso)-treated 9-WT wild type and hac701 with Maxwell 16 LEV Plant RNA Kit (Promega) run on the Maxwell 16 Instrument (Promega ...
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bioRxiv - Cell Biology 2022Quote: ... The transfection of wild-type MCF10A cells and the mutated derivatives was carried out with FuGene (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... 5 μL refolding reaction was diluted 1:5 into luciferase assay system mix (Promega), and luminescence was measured on the GloMax-20/20 Luminometer (Promega) ...
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bioRxiv - Neuroscience 2020Quote: ... Tissues were then stained using the following primary antibodies overnight: mouse anti-β Galactosidase (1:1000; Promega), rabbit anti-Er81 (1:5000 ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies: mouse anti-β-tubulin (E7, Developmental Studies Hybridoma Bank) and rabbit anti-Halo (G9281, Promega). Secondary antibodies ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The plasmid for β-arrestin-2 fused at the N-terminus to LgBiT was obtained from Promega custom assay services (plasmid CS1603B118) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... or with 0.05 µg GLP-1R-SmBiT and 0.05 µg LgBit-β-arrestin-2 (Promega, Hampshire, UK) plus 0.9 µg pcDNA3.1 for 24 hours ...
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Fatty acid binding proteins shape the cellular response to activation of the glucocorticoid receptorbioRxiv - Pharmacology and Toxicology 2021Quote: ... The GRE-SEAP reporter gene was from Clontech32 and the β-galactosidase reporter vector was from Promega. Human FABP3 ...