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Citations for Promega :
151 - 200 of 1032 citations for Adenovirus Type 5 Particles CMV β galatosidase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... were: β-tubulin (1:1500, TUJ1, Promega, G7121), Sox10 (1:300 ...
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bioRxiv - Molecular Biology 2023Quote: β-galactosidase activity was measured using β-Glo (Promega) essentially as described by Hook et al. ...
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bioRxiv - Genetics 2023Quote: ... 35µL of 1x β-galactosidase assay buffer (Promega) was added in unison to plated lysates and mixtures were incubated at 37°C for 40 min ...
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bioRxiv - Bioengineering 2024Quote: ... were: β-tubulin (1:1500, TUJ1, Promega, G7121), sox10 (1:300 ...
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bioRxiv - Biochemistry 2020Quote: ... magnetic beads were prepared by taking 100 μL of Streptavidin Magnesphere Paramagnetic Particles (Promega) and incubated with 5 μM of Spike-Fc-AviTag for 30 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... GST-tagged AtCFI59 was purified using agarose beads (MagneGSTTM Glutathione Particles; Promega, Madison, USA) and retained on the beads to pull-down purified HIS-tagged AtCFI25a protein in TBS buffer (50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Cancer Biology 2021Quote: 300 × 105 HEK 293T cells were split onto 6 well plates and the next day were mock transfected or transfected with 1.5 ug pTwist-CMV-SLC46A2-exFLAG using 4.5 uL FuGene6 transfection reagent (Promega). 24 h after transfection ...
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bioRxiv - Microbiology 2019Quote: ... We reverse-transfected cells in each well with 10 ng of pGL4.23-CMV/NP-NLuc or pNL1.1/NLuc (Promega) negative control and 100 ng of pGL4.23-CMV/NP-HaloTag or pcDNA3.3/eGFP negative control ...
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bioRxiv - Microbiology 2021Quote: ... Halo-V5-METTL14 and Halo-V5-WTAP were cloned into pHTN HaloTag CMV-neo vector (Cat# G7721, Promega) using Gibson assembly methods as previously described (7 ...
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bioRxiv - Neuroscience 2021Quote: ... neurons were co-transfected with TDP-43 expression constructs and the pGL4.50[luc2/CMV/Hygro] luciferase reporter (Promega). After 4 DIV ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were co-transfected with 50 ng of the Renilla luciferase transfection control plasmid pRL-CMV (Promega #E2261) and 1 μg of either the ERα reporter plasmid XETL (here referred to as ERE-Luc ...
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bioRxiv - Cell Biology 2022Quote: ... other cell types were transfected using Fugene HD (Promega) or Lipofectamine 2000 (Life technologies ...
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bioRxiv - Microbiology 2020Quote: ... Transfection efficiency was normalized through co-transfection of a lacZ reporter construct and determination of β-galactosidase activity using a commercial β-galactosidase enzyme assay system (Promega).
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Fatty acid binding proteins shape the cellular response to activation of the glucocorticoid receptorbioRxiv - Pharmacology and Toxicology 2021Quote: ... Media samples were taken to detect the secreted alkaline phosphatase reporter (GRE-SEAP) before the cells were lysed and assayed for β-galactosidase activity using the β-galactosidase enzyme assay system (Promega) to correct for differences in transfection efficiency ...
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bioRxiv - Immunology 2024Quote: ... qPCR targeting the β-globin gene was performed for each sample and evaluated alongside a β-globin calibration curve (human gDNA, cat# G1521, Promega).
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bioRxiv - Developmental Biology 2022Quote: ... Anti–β-galactosidase monoclonal mouse antibody (Promega, 1:500) was co-incubated with anti-mCherry polyclonal rabbit antibody (Bio Vision ...
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bioRxiv - Microbiology 2021Quote: ... luciferase values were normalized to β-galactosidase values (Promega). Luciferase values were then calculated as fold induction over the negative control (minus L ...
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bioRxiv - Microbiology 2021Quote: ... luciferase values were normalized to β-galactosidase values (Promega). Luciferase values were then calculated as fold increase over the negative control (minus L ...
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bioRxiv - Developmental Biology 2021Quote: ... anti-β-Galactosidase (1:1.000; Promega, 1:1000; abcam), anti-Cleaved Caspase-3 (1:500 ...
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bioRxiv - Cell Biology 2021Quote: ... β-cell apoptosis was measured using TUNEL (Promega, #G3250) followed by insulin staining (Dako ...
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bioRxiv - Cell Biology 2021Quote: ... 4 mM isopropyl β-d-1-thiogalactopyranoside (IPTG; Promega) was added to each RNAi well for induction of dsRNA synthesis and plates were incubated for 3-4 hours at 30°C and 155 rpm ...
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bioRxiv - Developmental Biology 2020Quote: ... together with 0.1 μg pRSV-β-gal plasmid (Promega) as a control for transfection efficiency ...
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bioRxiv - Developmental Biology 2020Quote: ... mouse anti-β-Gal (1:1000, Promega, Cat#: Z3781), rabbit anti-β-galactosidase (1:5000 ...
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bioRxiv - Cell Biology 2022Quote: ... mouse anti-β-galactosidase (1:1,000, Promega, Cat#: Z3781), rabbit anti-β-galactosidase (1:5,000 ...
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bioRxiv - Neuroscience 2023Quote: ... mouse Anti-β-Galactosidase (#Z3781, 1:200) from Promega; rabbit anti-SWS (1:1000 from Doris Kretzschmar) ...
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bioRxiv - Developmental Biology 2023Quote: ... and mouse anti-β-galactosidase (Promega #Z378, 1:1000) or rabbit anti-mCherry (BioVision ...
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bioRxiv - Synthetic Biology 2021Quote: ... pCR-U6-gRNA-miniCMV-Nluc was generated by replacing TdTomato with Nluc from pNLF1-C [CMV/Hygro] (Promega #N1361) using Gibson Assembly ...
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bioRxiv - Cancer Biology 2021Quote: ... reversely transfected into LNCaP or VCaP (treated with DHT or ETH) cells with a Renilla Luciferase control plasmid pGL4.75 [hRluc/CMV] (Promega) by using X-treme GENE HP DNA Transfection Reagent (Roche) ...
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bioRxiv - Cell Biology 2021Quote: ... with two plasmids – one containing the pRL Renilla luciferase gene under the control of the CMV promoter (# E2261, Promega) and the other containing the firefly luciferase gene under the control of the Pomc promoter (−646bp to +65bp ...
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bioRxiv - Molecular Biology 2020Quote: Prg4 enhancer/promoter-firefly luciferase reporters were co-transfected together with the pGL4.75 [hRlu/CMV] renilla luciferase reporter (Promega) into either superficial zone chondrocytes ...
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bioRxiv - Molecular Biology 2022Quote: ... were transfected with 320 ng 12x MS2-tagged repeat expression construct and 80 ng CMV-BFP plasmid DNA (for labeling transfected cells) mixed with 1.6 μl FuGENE HD (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Other plasmids used in this study include the pEGFP-N1 (Clonetech) and pRL-CMV vector (E226A) acquired from Promega. Cloning strategy for generating codon optimized SARS CoV-2 ORF7a in pCDNA-V5/His TOPO (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: HFF or HEK293 cells (3 × 104 cells per well of a 24-well plate or 1 × 105 cells per well of a 6-well plate) were reverse transfected with 0.5 μg of pCW57-CMV-KDEL-mCherry-GF using Fugene HD (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... The efficiency of biotinylation was subsequently evaluated by capture using streptavidin-coated paramagnetic particles (Promega). The first round of selection was performed using 400 nM of nanodisc-reconstituted MmFLVCR2 diluted in selection buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2020Quote: ... The partial frame of circ_0005962 containing the binding site or mutant binding site (wild-type or mutant-type) with miR-382-5p was amplified and cloned into the downstream of pGL4 vector (Promega, Madison, WI, USA) to generate circ_0005962-wt and circ_0005962-mut fusion plasmids ...
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bioRxiv - Cell Biology 2022Quote: ... (ii) wild-type cells transfected with mEGFP using Fugene6 (Promega), (iii ...
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bioRxiv - Microbiology 2020Quote: ... Cell fusion activity was quantitatively determined after 24 h by measuring firefly luciferase activity expressed by pT7EMCVLuc and was standardized with Rluc activity expressed by pRL-CMV by using the Dual-Glo luciferase assay (Promega) (27 ...
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bioRxiv - Molecular Biology 2021Quote: The pGL-AP-1 plasmid containing 6 consensus AP-1 binding sites was co-transfected with the pRL-CMV plasmid (Promega) into cells using TransIT 2020 transfection reagent (Mirus Bio ...
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bioRxiv - Molecular Biology 2021Quote: ... NM_001016) and SLC25A6 (accession number: NM_001636) were custom synthesized (IDT) and cloned into pRL-CMV Renilla luciferase vector (Promega, E2261) using oligos in Supplementary file 1 ...
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Redundant and specific roles of cohesin STAG subunits in chromatin looping and transcription controlbioRxiv - Cell Biology 2019Quote: ... the gRNA/Cas9 plasmid as well as the plasmid carrying homology arms and mAID-mClover neomycin cassette were transfected into HCT116 CMV-OsTIR1 cells using FuGene HD (Promega). After 6 days selection with 700 µg/ml G418 the cells were FACS-sorted for mClover expression and seeded into 96 well plates to grow single clones ...
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bioRxiv - Cell Biology 2021Quote: ... KIF3A-HaloTag and KIF9-v2-HaloTag were generated by sub-cloning KIF3A and KIF9-v2 into pHTN HaloTag CMV-neo (Promega). KIF13A ...
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bioRxiv - Biochemistry 2020Quote: ... The cells were then co-transfected with the corresponding pHR plasmid and lentiviral packaging plasmids (pMD and CMV) using Fugene HD (Promega). Cells were incubated for ~ 48 hours and virus was collected and filtered through a 0.45 mm filter ...
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bioRxiv - Bioengineering 2020Quote: VSV-G-pseudotyped lentiviral vectors were produced by co-transfecting HEK293T cells with lentiviral backbone plasmids and packaging plasmids (pHDM-Hgpm2, pHDM-tat1b, pRC/CMV-rev1b, pHDM-G) using Fugene HD (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... while the sequences for NSD2 or NSD3 PWWP1 domain was cloned in-frame into a pNLF1-C [CMV/Hygro] vector from Promega using the In-Fusion HD Cloning kit (Takara) ...
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bioRxiv - Neuroscience 2021Quote: ... Luciferase constructs were obtained by PCR amplifying the 3’UTR regions from cDNA extracts and cloning them into a modified phRL-CMV vector (Promega), as previously described (Favereaux et al. ...
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bioRxiv - Biochemistry 2019Quote: ... At that point they were co-transfected with desired pHR plasmid and lentiviral packaging plasmids (pMD and CMV) using Fugene HD (Promega). Virus was collected after approximately 48 hours ...
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bioRxiv - Cell Biology 2019Quote: ... or SURF4-rescue (defined above) were transfected with CMV-EPO-eGFP-p2A-A1AT-mCherry using Fugene HD transfection reagent (Promega). Twenty-four hours post-transfection ...
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bioRxiv - Immunology 2022Quote: 1×105 721.221 or 293T cells were plated in 24 well plates and transfected with 400 ng of pGL3 and 100 ng of pRL-CMV (Promega). Two days later ...
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bioRxiv - Molecular Biology 2022Quote: ... expressing PALLD and pNLF1-N [CMV Hygro] or pNLF1-C [CMV Hygro] vector (Promaga) expressing ANKRD1 were treated with 100 nM HaloTag NanoBRET 618 Ligand (Promega), whereafter signals were detected six hours later using a Synergy 4 instrument (BioTek) ...
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bioRxiv - Cell Biology 2023Quote: ... was obtained through transfecting HEK293T cells with these plasmids in combination with a second generation CMV lentiviral packaging system and FuGENE transfection agent (E2311, Promega).