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Citations for Promega :
251 - 300 of 5090 citations for 7 Chloro 1 3 dihydro 1 methyl 5 phenyl 2H benzo 1 4 diazepin 2 one 4 oxide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: Induction of apoptosis after 24 h of doxorubicin treatment was quantified by analyzing Caspase 3/7 activity using the Caspase-Glo® 3/7 3D assay (Promega, Madison, WI). The assay protocol was followed from the manufacturer’s website ...
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Class IIa HDACs inhibit cell death pathways and protect muscle integrity in response to lipotoxicitybioRxiv - Cell Biology 2023Quote: ... caspase 3 activity was measured using Caspase-Glo 3/7 assay (Promega, Alexandria, Australia). Quantification of viable cells was performed by staining with 7-aminoactinomycin D (7-AAD ...
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bioRxiv - Biochemistry 2019Quote: ... The washed beads were resuspended in 150 µl digestion buffer and incubated for 4 hours with 1 µg trypsin (Promega, catnr: V5111) at 37°C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The following day cells were trypsinized and seeded in 384-well white plate (20 µl/well) in DMEM F12 (no phenol red, 4% FBS) +/-HaloTag® NanoBRET™ 618 Ligand (1 µl/ml, Promega) and +/-compounds (DMSO concentration in each sample was kept the same) ...
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bioRxiv - Systems Biology 2023Quote: ... and digested using LysC 1:100 enzyme:proteins ratio for 4 hours (Wako sequencing grade, 125-05061) and trypsin 1:100 enzyme:proteins ratio for 16 hours (Promega sequencing grade, V5111). The digested proteins were then acidified with 10% (v/v ...
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bioRxiv - Systems Biology 2023Quote: ... Proteins were digested using LysC 1:100 enzyme:proteins ratio for 4 hours (Wako sequencing grade, 125-05061) and trypsin 1:100 enzyme:proteins ratio for 16 hours (Promega sequencing grade, V5111). The digested proteins were then acidified with 10% (v/v ...
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bioRxiv - Microbiology 2022Quote: ... cDNA was diluted either 1:5 or 1:10 for all qPCR experiments and GoTaq qPCR Master Mix (Promega) was used to amplify cDNA ...
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Bacteria Are a Major Determinant of Orsay Virus Transmission and Infection in Caenorhabditis elegansbioRxiv - Microbiology 2024Quote: ... Orsay Virus RNA1 level was quantified by qPCR using 1 μl of 1/5 diluted cDNA (GoTaq Promega A6001) and run on QuantStudio 3 Real Time PCR system ...
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Bacteria Are a Major Determinant of Orsay Virus Transmission and Infection in Caenorhabditis elegansbioRxiv - Microbiology 2024Quote: ... Orsay virus RNA1 level was quantified by qPCR using 1 μl of 1/5 diluted cDNA (GoTaq Promega A6001) and run on QuantStudio 3 Real Time PCR system (RNA1 qPCR primers GW194 and GW195)23.
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bioRxiv - Molecular Biology 2021Quote: ... transfected with 1 µg DNA and 3 µl FuGENE HD (Promega, E2311) in 150 µl Opti-MEM media according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... and anti-activated Caspase 3 (rabbit, Promega, catalog #G7481, 1:250, RRID:AB_430875), and appropriate secondary antibodies ...
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bioRxiv - Plant Biology 2022Quote: ... followed by 3 μL sequencing-grade trypsin solution (1 μg/μL, Promega), and the sample was incubated for 12 h at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... 30 μL of BrightGlo reagent solution (Promega, diluted 1:3 in water) was added to each well after 24 h of compound treatment ...
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bioRxiv - Biochemistry 2024Quote: ... 3 μL of 1:100 diluted NanoBRET Nano-Glo substrate (Promega N1571) was added ...
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bioRxiv - Biochemistry 2021Quote: ... 1 mM CaCl 2 and 10 ng/μL trypsin (Promega). Trypsin digestion was performed at 37 °C with rotation overnight and the beads were washed three times with PBS and distilled water again ...
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bioRxiv - Microbiology 2020Quote: ... followed by a reverse transcription using a universal degenerated oligo specific to all 5′ non-coding sequences of BTV-1 segments (BTV/Uni1; 5′ GTTAAAWHDB 3′) and the GoScript Reverse Transcription (RT) System (Promega, Madison, WI, USA). The cDNAs obtained for the segment 7 were then quantified with a real time quantitative PCR (qPCR) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 4% PEG-8000 (Promega), 2% FBS (Biosera) ...
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bioRxiv - Genomics 2023Quote: ... 4 mM DTT (Promega), and 1x CutSmart buffer (NEB ...
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bioRxiv - Genomics 2021Quote: ... 1 μL DNA and 5 μL of Master Mix 2x (Promega) (0.3 units of Taq Polymerase ...
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bioRxiv - Biochemistry 2022Quote: ... 5 μL Tris-phosphate buffer containing 1:50 Nanoglo substrate (Promega) was added to each well ...
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bioRxiv - Microbiology 2022Quote: ... which is approximately a 5:1 ratio of effector cells (Promega) to target cells ...
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bioRxiv - Biochemistry 2022Quote: ... 20 μl of 5 ng·μl−1 trypsin (sequencing grade, Promega®) in 50 mM ABC ...
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bioRxiv - Cancer Biology 2020Quote: ... Apoptosis was assessed with Caspase-Glo 3/7 assay (Promega) according to the manufacturer’s instruction.
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bioRxiv - Genetics 2021Quote: ... and Caspase-Glo® 3/7 Assay System (Promega, G8090), respectively ...
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bioRxiv - Neuroscience 2022Quote: ... in combination with Caspase-Glo® 3/7 Reagent (Promega) [33] ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The caspase 3/7 Glo assay (Promega, Madison, WI, USA) was performed on the white plates according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... and caspase-3/7 assay (Cat # G8090, Promega, Madison, WI) were performed using commercially available kits as per the instructions to assess proliferation and apoptosis ...
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bioRxiv - Cancer Biology 2023Quote: Apoptosis was measured by luminescent caspase 3/7 assay (Promega). Briefly ...
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bioRxiv - Plant Biology 2022Quote: ... as primary antibodies and anti-mousse IgG-HRP conjugated secondaries antibodies (Mouse IgG, HRP conjugate W402B, Promega; 1/5000 in TBST, 4 hours). As a loading control we used anti-tubulinα antibodies as primary antibodies (1/1000 in 5 % milk over-night) ...
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bioRxiv - Molecular Biology 2021Quote: ... One hundred nanograms of genomic DNA spiked with 1% (w/w) of unmethylated lambda DNA (Promega) was used for the library preparation ...
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bioRxiv - Molecular Biology 2023Quote: ... One-step RT-qPCR was performed using the GoTaq 1-Step RT-qPCR System (Promega, #A6020) and MicroAmp Fast Optical 96 Well Reaction Plates (Applied Biosystems ...
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bioRxiv - Zoology 2024Quote: One-step RT-qPCR was conducted using GoTaq 1-Step RT-qPCR System kit (Promega, France) in total reaction volume of 20 µl containing 2 µl of RNA extracts and 300 nM of the same forward and reverse primers as above ...
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bioRxiv - Plant Biology 2024Quote: ... 4 hours incubation at 37 °C) and 5 μg of trypsin (Trypsin Gold from Promega, in 50 mM TEAB pH 8.5 ...
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bioRxiv - Immunology 2023Quote: ... samples were diluted 1:1 with water and digested for 1.5 hours at 37 °C with 1 µg of LysC and overnight at 37 °C with 1 µg trypsin (Promega). The peptide mixture was acidified with trifluoroacetic acid (Merck ...
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bioRxiv - Neuroscience 2023Quote: ... UDP (at concentrations ranging from 1 nM to 1 mM) was then applied with a final concentration of 5 μM furimazine (NanoLuc Luciferase Assay, Promega). Luminescence was subsequently measured using a Victor X5 multi-label plate reader (PerkinElmer).
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bioRxiv - Biochemistry 2021Quote: ... The residual magnetic beads were then incubated for two hours at 4°C and for one hour at 16°C in 83 microlitres of buffer A200 with 20 Units RNasin (Promega), 12mM DTT and 16 micrograms of TEV-Protease ...
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bioRxiv - Cell Biology 2023Quote: The activity of the proteasome’s β5 sites was determined either by Succinyl(Suc)-LLVY-AMC (7-amido-4-methylcoumarin) fluorogenic substrate or by the Proteasome-Glo™ assay (Promega), a luciferase coupled assay ...
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bioRxiv - Genetics 2021Quote: Kidney pieces (1/3 of kidney) were homogenized in Passive Lysis Buffer (Promega) containing protease inhibitors (Thermofisher) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and ligated in a 1:3 vector:insert molar ratio using T4 ligase (Promega). Ligations were transformed into JM109 cells ...
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bioRxiv - Developmental Biology 2023Quote: ... and rabbit anti-Caspase-3 polyclonal antibody (1:250, Promega/Fisher, PR-G7481) overnight at 4 °C ...
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bioRxiv - Biophysics 2023Quote: ... Liposomes containing phosphatidylserine and PIP2 (3:1 ratio mol/mol) were purchased (Promega) and prepared to a final concentration of PIP2 at 25 μM ...
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bioRxiv - Cancer Biology 2022Quote: ... Nano-Glo Luciferase Assay Substrate furimazine (2 µl ml-1, Promega) was added and the nLuc released upon proteolytic cleavage of the receptor was measured on a plate reader Mithras LB 940 (Berthold Technologies ...
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bioRxiv - Plant Biology 2022Quote: ... Approximately 2-5 ng of purified genomic DNA was spiked with 1% (w/w) of unmethylated cl857 Sam7 Lambda DNA (Promega, Madison, WI) and sheared to about 400bp using Covaris M220 (Covaris Inc. ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Bound proteins were eluted with elution buffer 1 (50 mM Tris-HCl pH 7.5, 2 M urea, 5 ng/µL sequencing grade modified trypsin (Promega, Madison, WI, USA), 1 mM DTT ...
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bioRxiv - Synthetic Biology 2019Quote: ... ligated for 3 h at room temperature (RT) or overnight at 4°C using T4 ligase (Promega), and propagated in E.coli XL 10 Gold cells (Agilent) ...
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bioRxiv - Neuroscience 2023Quote: ... Blots were then incubated with blotting buffer containing 1:200 LgBiT for 1-2 h with rocking at RT (N2410, Promega). Next ...
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bioRxiv - Biochemistry 2023Quote: ... pLH3/DTX3L or pLH3/DTX3LΔN) and accessory plasmids pMD2g and psPAX2 (ratio 2:1:1) using ViaFect transfection reagent (Promega E498A). After cell incubation at 37°C for ∼16 h ...
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bioRxiv - Genomics 2021Quote: ... 1 U μl−1 RNasein (Promega), 0.1% IGEPAL CA-630 (Sigma)) ...
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bioRxiv - Immunology 2019Quote: ... In vitro pmel-1 T cell-mediated cytotoxicity assay with CytoTox-ONE Homogeneous Membrane Integrity Assay (Promega). protocols.io dx.doi.org/10.17504/protocols.io.6j4hcqw
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bioRxiv - Microbiology 2022Quote: ... 1 complete protease inhibitor tablet EDTA-free and 5 µl DNaseI (Promega), lysed using a cell disruptor and the insoluble fraction removed by centrifugation (15,000 xg ...