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Citations for Promega :
2751 - 2800 of 3351 citations for 7H Pyrrolo 2 3 d pyrimidine 7 3 5 bis O 2 4 dichlorophenyl methyl 2 C methyl b D ribofuranosyl 4 chloro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: Cells were blocked by pipetting 50ul of 5% Bovine serum albumin (BSA) (Promega, Cat: W3841) directly onto slides ...
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bioRxiv - Genetics 2021Quote: ... DNA ligation was performed using 5 μl HC T4 DNA ligase (Promega, 50-100 U) in 1X Ligation Buffer (Promega ...
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bioRxiv - Developmental Biology 2021Quote: ... the 5’-end of each mRNA was capped using the Ribo m7G Cap Analog (Promega), according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2021Quote: ... alkylated with iodoacetamid (final concentration of 5 mM) and digested overnight with trypsin (Promega V5113) in 1 M urea at an enzyme-to-substrate ratio of 1:40 ...
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bioRxiv - Plant Biology 2021Quote: ... each primer at 0.25 μM and 5 μL of 2X GoTaq qPCR Master Mix (Promega). The amplification program consisted of preincubation steps at 50°C for 2 min and 95°C for 10 min followed by 40 cycles of 95°C for 15 s and 60°C for 1 min ...
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bioRxiv - Microbiology 2020Quote: ... Cells (5 × 106) were then lysed using 100 μl of RIPA lysis buffer (Promega, WI) supplemented with 1 μl of proteinase inhibitor and 10 μl of phenylmethylsulfonylfluoride ...
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bioRxiv - Molecular Biology 2022Quote: ... terminated by incubation on ice and diluted 1:5 in Glo Lysis Buffer (Promega # E2661). 25 μL of prepared Nano-Glo reagent (Promega # N1120 ...
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bioRxiv - Systems Biology 2019Quote: ... Half of the cDNA (5 μl) was amplified by PCR using Pfu Polymerase (Promega, M7745) with the cycling conditions (95°C for 2 min ...
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bioRxiv - Cancer Biology 2019Quote: ... before dilution with 120 μL 0.04 × ProteaseMax and 5 μg/μL sequencing grade trypsin (Promega) in PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... Survival was determined after 5 days of incubation using the Cell Titre-Blue assay (Promega). All cell lines were treated with 4 and 8 µM Erastin and 3 and 6 µM RSL3 ...
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bioRxiv - Plant Biology 2022Quote: ... and 0.3 µl of Taq polymerase (5 u/µl) provided by Promega (Madison, Wisconsin, U.S.A.). Variations in MgCl2 (0.5–1 µl ...
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bioRxiv - Biochemistry 2024Quote: ... Luciferase activity was measured 5 h later using Bright-Glo Luciferase Assay System (Promega, E2620) on a Tristar 5 microplate reader (Berthold).
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bioRxiv - Cancer Biology 2024Quote: ... After 5 days cells were collected per manufacturer’s instructions using either CellTiterGlo® (Promega, G7570) to measure viability by assessing ATP concentrations ...
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bioRxiv - Cell Biology 2023Quote: ... Purified 5 μg of pAd/CMV/V5-DEST plasmid was transfected using Fugene HD (Promega) when the 293A cells were 80% confluent ...
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Mitochondrial Apolipoprotein MIC26 is a metabolic rheostat regulating central cellular fuel pathwaysbioRxiv - Cell Biology 2023Quote: ... cDNA synthesis from 5 µg RNA was performed using the GoScriptTM Reverse Transcriptase Kit (Promega). Next ...
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bioRxiv - Plant Biology 2023Quote: ... 5’RACE products were gel-purified and cloned in pGem®-T Easy vector (Promega). Independent clones were randomly chosen and sequenced by Sanger sequencing method.
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bioRxiv - Biochemistry 2023Quote: ... with 25 μL of diluted reactions (diluted 1:5 in Glo Lysis Buffer; Promega # E2661) and incubated at room temperature for 5 min ...
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bioRxiv - Biochemistry 2023Quote: ... 10 μg of the isolated recombinant bacmid and 5 μL Fugene HD transfection reagent (Promega) were diluted in 150 μL Sf-900™ III SFM ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were digested by adding 5 μL of sequencing grade trypsin (0.1 mg/mL) (Promega) for three h at 37 °C ...
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bioRxiv - Synthetic Biology 2021Quote: ... pCR-U6-gRNA-miniCMV-Nluc was generated by replacing TdTomato with Nluc from pNLF1-C [CMV/Hygro] (Promega #N1361) using Gibson Assembly ...
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bioRxiv - Microbiology 2020Quote: ... ratio 50:1 (protein:rLysC) for 3h/37°C and then digested with Sequencing Grade Modified Trypsin (#V5111, Promega, France) ratio 50:1 (protein:trypsin ...
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bioRxiv - Cell Biology 2020Quote: ... the gel pieces were covered with 200 μl of 20 ng/μl of sequence grade trypsin/Lys-C (Promega) in 50 mM ammonium bicarbonate pH8 buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... Urea-solubilized pellet was then diluted to 2M urea using NaCl/Tris solution (150mM NaCl, 50mM Tris pH 8.0) and digested overnight at 37°C with 10ug of trypsin GOLD (Promega). The following day ...
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Osh6 requires Ist2 for localization to the ER-PM contacts and efficient phosphatidylserine transportbioRxiv - Cell Biology 2020Quote: Proteins were digested overnight at 37°C in 20 ml of 25 mM NH4HCO3 containing sequencing-grade trypsin (12.5 mg/ml; Promega). The resulting peptides were sequentially extracted with 70% acetonitrile and 0.1% formic acid ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were digested with LysC (Wako) (substrate:enzyme=100) overnight at 37°C and then with sequencing grade trypsin (Promega) (substrate:enzyme=100 ...
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bioRxiv - Microbiology 2020Quote: ... Cytosine tails (C-tails) were added to 1 µg sheared DNA using terminal deoxynucleotidyl transferase (TdT, Promega, Madison, WI). Transposon containing fragments were amplified in a PCR containing DNA from the TdT reaction as template and primers specific to the ColE1 site on the 5’ end of the transposon ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were washed twice (3000 x g for 5 min at 4°C) and resuspended in RNAse-free PBS with 1% BSA and 0.5 U/ul RNase inhibitor (Cat. N2615, Promega). Cells were then used for the 10x Genomics Chromium Single Cell System (10x Genomics ...
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bioRxiv - Neuroscience 2021Quote: ... cells were washed twice (3000 x g for 5 min at 4°C) and resuspended in RNAse-free PBS with 1% BSA and 0.5 U/ul RNase inhibitor (Cat. N2615, Promega). Cells were then used for the 10x Genomics Chromium Single Cell System (10x Genomics ...
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bioRxiv - Molecular Biology 2021Quote: ... Fujifilm) and then incubated for 8 h at 37 °C with sequence-grade trypsin (1□g; Promega, Madison, Wisconsin). Peptides were desalted on Ultra Micro Spin Columns C18 (The Nest Group Inc. ...
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bioRxiv - Immunology 2021Quote: ... The cells were incubated for an additional 6 hours at 37°C before 75μl of Bio-Glo Luciferase Assay Reagent (Promega) was added to each well ...
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bioRxiv - Biochemistry 2020Quote: ... and proteins were finally subjected to overnight digestion with mass spec grade Trypsin/Lys-C mix (Promega, Madison, WI). Digested proteins were finally desalted using a C18 TopTip (PolyLC ...
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bioRxiv - Cancer Biology 2019Quote: ... was added and incubated at 37 °C for 20 min before the addition of ADP-Glo reagent (5μL) (Promega) for an additional 20 min incubation at room temperature ...
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bioRxiv - Molecular Biology 2019Quote: ... and C-terminally truncated VP16 (VP16ΔC) proteins were synthesized using a rabbit TNT quick-coupled transcription/ translation system (Promega) with 35S-labeled methionine ...
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bioRxiv - Systems Biology 2019Quote: ... with N-and C-terminus tagging configurations (NB MCS1, NB MCS2, NB MCS3, NB MCS4) were obtained from Promega, each containing an HSV-TK promoter for protein expression in mammalian cells and an ampicillin resistance marker for bacterial selection of transformed cells ...
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bioRxiv - Cancer Biology 2019Quote: ... and proteins were finally subjected to overnight digestion with mass spec grade Trypsin/Lys-C mix (Promega, Madison, WI). Finally ...
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bioRxiv - Cancer Biology 2019Quote: ... and cultured for 72 h at 37°C before viability assay using the ATP-based CellTiter-Glo (CTG) 2.0 Assay (Promega). Luminescence was read with the LMAX II luminometer (Molecular Devices) ...
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bioRxiv - Microbiology 2019Quote: ... Samples were then incubated overnight at 37 °C with 500 ng Sequencing Grade Modified Trypsin (Promega, Madison, WI, USA). To achieve the complete digestion of the peptides ...
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bioRxiv - Cancer Biology 2019Quote: ... incubated at 37 °C overnight and the luciferase activity was measured using the Bio-Glo Luciferase Assay System (Promega) on the EnVision Multilabel Plate Reader (Perkin Elmer) ...
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bioRxiv - Neuroscience 2020Quote: ... in 50 µL of 100 mM ABC for 3h at 1200 rpm at 37°C followed by overnight trypsin digestion using 0.2 µg of trypsin (Promega) per sample in 100 mM ABC without 0.1% SDC ...
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bioRxiv - Biochemistry 2021Quote: ... The next day the sample was further diluted to 1 M urea and 750 ng Glu-C (Promega, V1651) were added and incubated for another 8 hours at 37°C.
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ARL3 Mediates BBSome Ciliary Turnover by Promoting Its Outward Diffusion through the Transition ZonebioRxiv - Cell Biology 2021Quote: ... Five micrograms of RNA were reverse transcribed at 42 °C for 1 h using M-MLV Reverse Transcriptase (Promega) and oligo(T)18 primers (Takara) ...
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bioRxiv - Cell Biology 2021Quote: ... A total of 0.2 mg of protein was subjected to overnight digestion with 8.0 µg of mass spec grade Trypsin/Lys-C mix (Promega). Following digestion ...
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bioRxiv - Plant Biology 2020Quote: ... Tryptic in-gel digestion of gel slices was performed overnight at 37°C in 50 mM ammonium bicarbonate using 0.1 µg trypsin (Promega) per gel band ...
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bioRxiv - Neuroscience 2020Quote: Plates were incubated for 24 hours at 37°C and assayed for FL and RL using the Dual-Luciferase Assay System and following manufacturer’s instructions (E1980, Promega). Liquid handling and luciferase assay were performed using the integrated automated screening platform (Beckman Coulter ...
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bioRxiv - Microbiology 2021Quote: ... 0.025% deoxycholate and 200 µg.mL−1 RNase A at 37°C for 20 min to lyse the cells and Protein Precipitation Solution (Promega) was added ...
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bioRxiv - Microbiology 2020Quote: ... with the required amount of cells (cell number equal to the number of wells multiplied by 1.5 × 104).The effector and target cells were mixed 20 h post-transfection at 37°C in fresh medium containing 60μMmembrane-permeable substrate for Renilla luciferase (RL) (Enduren Live Cell Substrate; Promega)1 h prior to mixing ...
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bioRxiv - Cancer Biology 2021Quote: ... and the sample was digested at 37 °C with sequencing-grade trypsin (Promega; 1:50 protease:protein w/w ratio) or at 25°C with Glu-C (Promega ...
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bioRxiv - Cell Biology 2021Quote: ... then samples were digested overnight at 37°C on a rotator with modified trypsin (1:20 trypsin:sample ratio) (Promega). Peptides were desalted using SepPak C18 columns (Waters ...
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bioRxiv - Cancer Biology 2022Quote: ... before the addition of digestion buffer (50 mm Tris) containing 0.8 μg Lys-C (Wako, 129–02541) and 0.8 μg Trypsin-gold (Promega, V5280). Enzymatic digestions proceeded under agitation (400 rpm ...
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bioRxiv - Plant Biology 2022Quote: ... Samples were diluted with 900 µl of 25 mM ammonium bicarbonate and digested overnight at 37°C in the presence of 0.35 µg of sequencing grade modified porcine trypsin (Promega). Peptides were vacuum-dried in a centrifugal evaporator to approximately 250 µl ...