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Citations for Promega :
2701 - 2750 of 3351 citations for 7H Pyrrolo 2 3 d pyrimidine 7 3 5 bis O 2 4 dichlorophenyl methyl 2 C methyl b D ribofuranosyl 4 chloro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... proteins were digested with chymotrypsin (5 ng/μL) at 25ºC overnight (Trypsin gold, Promega). Digestion was stopped by addition of 5% formic acid and peptides extracted twice with 70% acetonitrile and 5% formic acid (10 min sonication) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... for 30 minutes then with 5 units/1 L RQ1 RNase-free DNase (Promega) for 10 minutes at room temperature ...
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bioRxiv - Genomics 2024Quote: ... Trypsin digestion was done by adding 5 µL reductively methylated trypsin (Promega, Madison, USA) to each sample and incubating the samples overnight at 37°C ...
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bioRxiv - Systems Biology 2023Quote: ... 5 x 106 cells were electroporated with 1 μg of NanoLuc pNL3.1 (Promega, N1031) vector and 0.2 μg of control vector ...
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bioRxiv - Neuroscience 2023Quote: ... transferred to opaque 96-well plates containing 5 μM furimazine (NanoLuc Luciferase Assay, Promega), and bathed in 5-HT at various concentrations (ranging from 0.1 nM to 1 mM) ...
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bioRxiv - Immunology 2023Quote: ... Luciferase Assay System (E1501) and Passive Lysis 5 × Buffer (E1941) were purchased from Promega. C1q recombinant protein (A400 ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 μm tissue scrolls were processed using the Maxwell RSC RNA FFPE instrument (Promega). RNA concentration and quality was determined using a TapeStation automated electrophoresis instrument (Agilent) ...
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bioRxiv - Cell Biology 2024Quote: ... the 5′-end of each mRNA was capped using Ribo m7G Cap Analog (Promega), according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... cells were incubated with 5-100 pM of JF549-HTL (Cat. No. GA1110, Promega) and 50 nM Hoechst 33342 for an hour in complete medium ...
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bioRxiv - Cell Biology 2019Quote: Proteins were incubated overnight at 37°C with 20 µl of 25 mM NH4HCO3 containing sequencing-grade trypsin (12.5µg/ml, Promega) at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... The sample was digested for 2 h with 0.5 µg of endoproteinase lysine-C (Wako chemicals) and then digested with 0.5 µg of trypsin (Promega) overnight ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were incubated at 37°C for 72 hours and cell viability was measured using Cell TiterGlo (Promega). Percent viability of treated wells was calculated by normalizing to the average Cell TiterGlo values from untreated wells.
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bioRxiv - Cell Biology 2019Quote: ... RNAs were subsequently generated by in vitro transcription overnight at 37°C using RiboMAX transcription kits (Promega Corporation). After RQ1 DNAse digestion of the template cDNA ...
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bioRxiv - Genomics 2020Quote: ... Beads were resuspended in 50μL 100mM AMBIC and incubated overnight at 37°C with 400ng Trypsin (Promega V5280). Beads were supplemented with an additional 400ng Trypsin and incubated for a further 4 hours at 37°C ...
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bioRxiv - Cell Biology 2019Quote: ... Samples were diluted two-fold again and incubated overnight at 37 °C with trypsin (sequencing grade modified, Promega) at a 1:50 (w/w ...
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bioRxiv - Cell Biology 2019Quote: ... Samples were diluted two-fold with 20 mM HEPES pH 8.0 and proteins digested with Lys-C (Promega) at a protease/protein ratio of 1:100 (w/w ...
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bioRxiv - Biochemistry 2020Quote: ... Enzymatic digestion of was performed overnight at 37°C by the addition of sequencing grade trypsin (Promega, Wisconsin) (100 ng per sample ...
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bioRxiv - Cancer Biology 2021Quote: ... the protein extracts were then incubated overnight at 37°C with 20 μl of 25mM NH4HCO3 containing sequencing-grade trypsin (12.5μg/ml, Promega). The resulting peptides were desalted using ZipTip µ-C18 Pipette Tips (Millipore ...
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bioRxiv - Molecular Biology 2022Quote: ... at 72°C for 20min and then TA cloned into the pGEM-T Easy vector (Promega, Madison, WI). The cloned PCR products were Sanger Sequenced with M13 Forward (5’-TGTAAAACGACGGCCAGT-3’ ...
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bioRxiv - Microbiology 2020Quote: ... emission 410 nm) was immediately measured every 15 minutes at 37°C using a GloMax plate reader (Promega).
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bioRxiv - Molecular Biology 2022Quote: ... the proteins were digested overnight at 37 °C with 6 ng μL-1 of Asp-N (Promega, UK). Peptides were extracted in 2 % v/v formic acid ...
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bioRxiv - Microbiology 2022Quote: ... Proteins on magnetic beads were digested overnight at 37°C with 1μl (0.2 μg/μL) of trypsin (Promega) in a 25-mM NH4HCO3 buffer per sample ...
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bioRxiv - Molecular Biology 2019Quote: 1875 ng of circular plasmids were incubated at 37°C with 50 U of T3 RNA Polymerase (Promega) in a transcription buffer (40 mM Tris-HCl pH 7.9 ...
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bioRxiv - Microbiology 2019Quote: ... were reverse transfected with 50 ng pCAGEN-HA plasmid and 12.5 ng pcDNA3.1+/C-(K)DYK protease-plasmid using Fugene 6 (Promega). After 24 h ...
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bioRxiv - Cell Biology 2020Quote: ... The proteins were then digested overnight at 37°C with 0.2 μg of modified sequencing grade trypsin (Promega) in 50 mM ammonium bicarbonate ...
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bioRxiv - Cell Biology 2021Quote: ... the samples were diluted to 1.5 M urea using 20 mM HEPES (pH 8.5) and digested at 30°C overnight with trypsin (Promega) at a ratio of 70:1 (w/w) ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were digested on the column with 0.25 µg of Trypsin/Lys-C protease mix (Promega; cat. V5071), and peptides eluted for mass spectrometry.
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bioRxiv - Developmental Biology 2021Quote: ... into 100 mM ammonium bicarbonate and digested overnight at 37 °C with mass spectrometry grade trypsin gold (Promega) at a 1:50 trypsin:protein ratio ...
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bioRxiv - Biophysics 2021Quote: ... Human Gβ with a C-terminal 15-amino-acid polypeptide linker followed by a HiBiT (peptide 86, Promega) and human Gγ were cloned into pFastBac vector ...
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bioRxiv - Microbiology 2020Quote: ... where the samples were destained and subjected to in-gel proteolytic digestion with trypsin/Lys-C mixture (Promega). The digests were extracted ...
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bioRxiv - Microbiology 2022Quote: ... The concentrated phages were next treated for 30 min at 37°C with 10 μL of DNAse (Promega) and 2.5μL of RNAse (Macherey-Nagel ...
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bioRxiv - Biophysics 2022Quote: ... and the two halves were digested with either Sequencing Grade Modified Trypsin or endoproteinase Glu-C (both Promega, Madison ...
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bioRxiv - Molecular Biology 2023Quote: ... Enzymatic digestion was performed at 37°C and overnight with 500 ng trypsin (Promega, Charbonnieres les Bains, France). Peptide mixtures were then desalted on C18 spin-column and dried in a Speed-Vacuum.
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bioRxiv - Neuroscience 2024Quote: ... Prior to overnight digestion at 37 °C using modified porcine trypsin (Mass Spectrometry Grade, Promega, Madison, WI, USA) at an enzyme–protein ratio of 1:80 ...
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bioRxiv - Biochemistry 2023Quote: ... then reverse transcribed for 1 hr at 37 °C using M-MLV reverse transcriptase (Promega, Madison, WI; M1705) in the presence of RNase inhibitor (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were then acidified with 12-21% aqueous phosphoric acid prior to digestion with Trypsin/Lys-C (Promega) and then added immediately to an S-Trap column (Protifi ...
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bioRxiv - Biochemistry 2023Quote: ... The samples were further processed by digesting them overnight with a Trypsin/Lys-C mix (Promega, Madison, USA) at a 50:1 substrate:enzyme ratio (w/w ...
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bioRxiv - Microbiology 2023Quote: ... Trypsin digest was allowed overnight at 37°C by the addition of 1 μg of trypsin (#V5111, Promega) to each sample ...
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bioRxiv - Microbiology 2023Quote: ... The pellet was resuspended in 0.1M TEAB and overnight (∼16 h) digestion with trypsin/Lys-C mix (1:25 protease:protein; Promega) at 37° C was performed with constant mixing using a thermomixer ...
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bioRxiv - Physiology 2023Quote: ... Samples were incubated for 60 minutes at 47°C with sequencing grade trypsin (Promega, San Luis Obispo, CA) dissolved in 50 mM TEAB at a 1:25 (w/w ...
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bioRxiv - Molecular Biology 2023Quote: Reduced and alkylated proteins were prepared by in-gel digesting overnight at 37°C using 100ng trypsin (Promega). The supernatant was dried by vacuum centrifugation and the samples were resuspended in 0.1% trifluoro acetic acid ...
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bioRxiv - Microbiology 2023Quote: ... The concentrated phages were next treated for 30 min at 37°C with 10 µl of DNAse (Promega) and 2.5 µl of RNAse (Macherey-Nagel ...
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bioRxiv - Neuroscience 2023Quote: ... 2018) resulting in two fractions after sequential proteolysis with first Lys-C (Wako Chemicals) followed by trypsin (Promega). Peptides were analyzed on a Q Exactive HF mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... and then subjected to overnight digestion at 37°C with sequencing-grade modified trypsin (Promega, Madison, WI, USA). The peptides were extracted ...
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bioRxiv - Plant Biology 2023Quote: ... Luciferase activity was then measured at room temperature (below 25 °C) using the Luciferase Assay System (Promega Corporation) in a Glomax luminometer (Promega Corporation ...
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bioRxiv - Cell Biology 2023Quote: ... The digestion was performed in a 50 mM TEAB buffer with 0.2 μg of Lys-C (Wako Chemicals) and 0.4 μg of trypsin (Promega). Trifluoroacetic acid (TFA ...
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bioRxiv - Systems Biology 2023Quote: ... and digested according to the manafacturer’s protocol for 1 hour at 50 °C by Trypsin Platinum (Promega, WV), digested material was dried in speedvac (Eppendorf ...
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bioRxiv - Biochemistry 2024Quote: ... were digested overnight at 37 °C using trypsin with a 1:20 trypsin to sample ratio (Promega, #V5117). The next day ...
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bioRxiv - Microbiology 2024Quote: ... and then incubated for an additional hour at 55°C after adding 20 mg/ml proteinase K (Promega). DNA was extracted using the ZymoBIOMICS DNA Miniprep Kit (Zymo Research) ...
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bioRxiv - Microbiology 2024Quote: ... Then they were diluted again to ¼ and digested overnight at 37 °C with sequencing grade-modified trypsin (Promega) at a ratio of 1:50 ...