Labshake search
Citations for Promega :
2651 - 2700 of 3657 citations for Borrelia burgdorferi sensu stricto B31 CRASP 1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genetics 2019Quote: ... As secondary antibodies horseradish peroxidase (HRP)-conjugated α-mouse or α-rabbit IgG (Promega, 1:4000) were used ...
-
bioRxiv - Genetics 2020Quote: ... 1 microgram of total genomic DNA (gDNA) was added with 0.1% of unmethylated lambda DNA (Promega), followed by one-hour digestion with three enzymes (MspI ...
-
bioRxiv - Cell Biology 2022Quote: ... After two days, images were captured by CCD (TANON 5200, China) after 1 mM luciferin (Promega) was sprayed onto the leaves ...
-
bioRxiv - Immunology 2022Quote: ... Supernatants were harvested and subjected to Caspase-Glo 1 inflammasome assay following manufacturer’s protocol (G9951, Promega). Luminescence from caspase-1 activity was detected with a FLUOstar Omega plate reader (BMG ...
-
bioRxiv - Cancer Biology 2022Quote: ... On-bead tryptic digestion was performed with 1 μg MS-grade TPCK trypsin (Promega, Madison, WI) dissolved in 200 μl of 50 mM ammonium bicarbonate pH 8.3 overnight at 37°C ...
-
bioRxiv - Cancer Biology 2022Quote: ... 20 µL of PBS containing 10 μg/mL Hoechst and 1/4000 CellTox Green Dye (Promega) were dispensed per well 8 h prior to imaging at Opera Phenix (Perkin Elmer ...
-
bioRxiv - Genomics 2022Quote: ... Sections were then incubated with a horseradish peroxidase-labeled anti-rabbit antibody (1:300, Promega, W401B) for 1 hour at room temperature ...
-
bioRxiv - Genomics 2022Quote: ... the NE1 and Wash buffers were supplemented with 1 U/μL of RNasin Ribonuclease Inhibitor (Promega).
-
bioRxiv - Plant Biology 2022Quote: ... for 2 h as primary antibody and peroxidase-conjugated goat anti-rabbit antibody (1:10,000; Promega) for 1 h with 5 times 10 min washes in-between the incubations ...
-
bioRxiv - Microbiology 2022Quote: ... 1 μL of ligation product was amplified using PCR with a GO Taq DNA polymerase (Promega) and Illumina primers (P7 5’-CAAGCAGAAGACGGCATACGAGATAGACCGGGGACTTATCATCCAACCTGT-3’ ...
-
bioRxiv - Molecular Biology 2023Quote: RNA isolated from cells (1 μg) was digested using the RQ1 RNase-Free Dnase (M6101, Promega) and reverse-transcribed using the High-Capacity cDNA Reverse Transcription Kit (4368814 ...
-
bioRxiv - Microbiology 2022Quote: ... The solution was diluted to below 1 M urea and digested using sequencing grade Trypsin (Promega). The proteolytic peptides were finally desalted using an Oasis HLB solid phase extraction well plate (Waters ...
-
bioRxiv - Microbiology 2022Quote: ... Reactions were performed at 1/5th volume with the inclusion of Transcend Biotin-Lysyl-tRNA (Promega) to allow antibody independent detection of all translated products ...
-
bioRxiv - Plant Biology 2022Quote: ... 500 ng of total RNAs were treated with 1 unit of RQ1 RNase-Free DNase (Promega) for 30 min at 37 °C ...
-
bioRxiv - Plant Biology 2024Quote: ... the membranes were incubated with an HRP-conjugated secondary antibody (Promega, Cat# W4021, 1:10,000 dilution) in blocking solution ...
-
bioRxiv - Neuroscience 2024Quote: ... 0.1-1 µg of RNA was reverse transcribed into cDNA using M-MLV reverse transcriptase (Promega) and oligo(dT ...
-
bioRxiv - Zoology 2024Quote: One-step RT-qPCR was conducted using GoTaq 1-Step RT-qPCR System kit (Promega, France) in total reaction volume of 20 µl containing 2 µl of RNA extracts and 300 nM of the same forward and reverse primers as above ...
-
bioRxiv - Molecular Biology 2023Quote: ... Cells were resuspended in 1 mL of the same buffer containing protease inhibitor cocktail (Promega, USA), and then transferred to 2 mL lysing matrix B tubes containing 0.1 mm diameter silica beads (MP Biomedicals ...
-
bioRxiv - Microbiology 2024Quote: ... Viral RNA was quantified by real-time RT-qPCR (GoTaq 1 step RT-qPCR kit, Promega). Quantification was provided by serial dilutions of an appropriate T7-generated synthetic RNA standard ...
-
bioRxiv - Cell Biology 2024Quote: ... Nano-Glo assay reagent consisted of a 1:1000 dilution of Nano-Glo substrate (Promega; N1110) in buffer (100mM 2-(N-Morpholino)ethanesulfonic acid hydrate (M8250 ...
-
bioRxiv - Cancer Biology 2024Quote: 1 microgram total RNA extracted using ReliaPrep™ RNA Cell Miniprep System (Promega, Madison, WI, USA), were checked using the Agilent-2100 Bioanalyzer System and subjected to library preparation utilizing the TruSeq stranded total RNA Gold library preparation kit with RiboZero Gold treatment and unique dual indexes according to the manufacturer’s instructions (Illumina Inc. ...
-
bioRxiv - Molecular Biology 2024Quote: ... cDNA was synthesized with 1 μg/μL of RNA concentration using MMLV-RT enzyme (Promega, USA). HvHsfA6a gene specific and Actin primers designed by using Primer express 3.0 software (Thermoscientific USA ...
-
bioRxiv - Cell Biology 2024Quote: ... cells were transfected with TFG-mEGFP-FLAG at 1 µg/µL using Fugene (Promega, cat # PRE2311) and left to express for 24 hours ...
-
bioRxiv - Molecular Biology 2023Quote: ... 1/20 of the cDNA was amplified by PCR using the GoTaq DNA polymerase mix (Promega) with specific antigenome primers (Appendix Table S1) ...
-
bioRxiv - Developmental Biology 2024Quote: ... They were then embedded on the side position in 1% low melting agarose (Promega, Cat# V2111) in a glass bottom 60μ-Dish (35mm high ...
-
bioRxiv - Bioengineering 2024Quote: ... RT-qPCR was performed using the GoTaq Probe 1-Step RT-qPCR System (Promega Cat# A6120). RT-qPCR primers and probes were listed in Supplementary Data 2.
-
bioRxiv - Microbiology 2023Quote: ... 15 µl samples were allowed to cool to room temperature and 1 µl RQ1 DNase (Promega) diluted to 0.1 U/µl in storage buffer (10 mM Hepes pH 7.5 ...
-
bioRxiv - Molecular Biology 2023Quote: ... Luminescence was measured at 1 day post infection using the Nano-Glo Luciferase Assay System (Promega) on the Envision plate reader after cell lysis with 1X passive lysis buffer (E1941 ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... the cells were transfected with 1 or 1.5 μg plasmid DNA using FuGene HD (Promega, #E2311) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2023Quote: ... the cells were co-transfected using Fugene HD transfecting reagent at a 1:3 ratio (Promega) along with pRL-TK Renilla (Renilla Luciferase ...
-
bioRxiv - Microbiology 2022Quote: Viral RNA was quantified by real-time RT-qPCR (GoTaq 1 step RT-qPCR kit, Promega). Quantification was provided by serial dilutions of an appropriate T7-generated synthetic RNA standard ...
-
bioRxiv - Neuroscience 2023Quote: ... animals were anesthetized with isoflurane inhalation and 10uls of 1% wheat germ agglutinin (WGA)-AF488 (Promega), diluted in 1%DMSO ...
-
bioRxiv - Plant Biology 2023Quote: ... LUC images were taken 2-3 days after infiltration by applying 1 mM Beetle luciferin (Promega) on adaxial leaf surface.
-
bioRxiv - Cell Biology 2023Quote: ... primers designed for the gene of interest (Table 1) and GoTaq(R) qPCR Master Mix (Promega). Samples were run on an AB 7300 Real Time PCR System (Applied Biosystems ...
-
bioRxiv - Biochemistry 2023Quote: ... the membrane was visualized using 500 μL of 1:1000 NanoGlo substrate (Promega, catalogue number: N1110).
-
bioRxiv - Molecular Biology 2023Quote: ... First-strand cDNA synthesis was performed from 1 µg RNA using M-MLV Reverse Transcriptase (Promega) and random hexamer primers (Sigma-Aldrich ...
-
bioRxiv - Plant Biology 2023Quote: ... Samples were digested via addition of two aliquots of sequencing-grade trypsin (Promega, 1:50 (w:w)) at two different sample dilutions ...
-
Deciphering the polycistronic nature of Mycobacterium tuberculosis lipoproteins of unknown functionsbioRxiv - Microbiology 2023Quote: ... The cDNA synthesis was performed using 1 μg of RNA with M-MLV reverse transciptase (Promega) (RT+ ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... and incubated for 15’ with Proteinase K (1:500, in 1XPBS, Promega B.V., Leiden, The Netherlands) at 37°C ...
-
bioRxiv - Immunology 2023Quote: ... 1 ng pEF1α-Renilla and 130 ng plasmid of interest and 0.66 µl FuGENE HD (Promega) diluted to 10 µl total volume with OptiMEM ...
-
bioRxiv - Microbiology 2023Quote: ... viral RNA was quantified by real-time RT-qPCR (GoTaq 1 step RT-qPCR kit, Promega). Quantification was provided by serial dilutions of an appropriate RNA standard ...
-
bioRxiv - Cancer Biology 2023Quote: ... or MEK inhibitor U0126 (1, 10, and 50 µM for 4 h; Promega, Madison, WI, USA). A corresponding volume of dimethyl sulfoxide (Nacalai Tesque ...
-
bioRxiv - Biophysics 2023Quote: ... cells expressing mGluR3 with Halo tag in ICL2 were labeled with 1 µM CA-JF549 (Promega) and 3 µM CA-JF646 (Promega) ...
-
bioRxiv - Microbiology 2023Quote: ... Complimentary DNA was prepared from 1 µg of RNA using a Reverse Transcription Systems Kit (Promega). Quantitative PCR (qPCR ...
-
bioRxiv - Molecular Biology 2023Quote: ... to the 1x PBS. PD-1/PD-L1 blockade bioassay (cat. J1250) was purchased from Promega.
-
bioRxiv - Neuroscience 2023Quote: ... At DIV12 cells are lysed in Passive lysis buffer (diluted 1:5 in MilliQ-water, Promega) for 15min and dual luciferase assay performed using home-made reagents according to Baker and Boyce (63 ...
-
bioRxiv - Microbiology 2023Quote: RT-qPCR was performed using the GoTaq 1-Step RT-qPCR kit (Promega Cat. No. A6020) using nifH and 16S rRNA (reference gene ...
-
bioRxiv - Cell Biology 2023Quote: ... Quantitative Realtime PCR (qRT-PCR) was performed using GoTaq® 1-Step RT-qPCR kit (Promega) with primers specific for PEX1 (RE7039-RE7040 ...
-
bioRxiv - Molecular Biology 2023Quote: ... One-step RT-qPCR was performed using the GoTaq 1-Step RT-qPCR System (Promega, #A6020) and MicroAmp Fast Optical 96 Well Reaction Plates (Applied Biosystems ...
-
bioRxiv - Molecular Biology 2023Quote: ... After the translation reaction was quenched by adding 30 μl of 1× Passive Lysis Buffer (Promega), 10 μl of the mixture was placed on a 96-well white assay plate (Coster) ...