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Citations for Promega :
2501 - 2550 of 3657 citations for Borrelia burgdorferi sensu stricto B31 CRASP 1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2020Quote: 0.4 µL GoScriptTM RT Mix for 1-Step RT-qPCR (Promega, Cat # A6120 and A6121)
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bioRxiv - Microbiology 2019Quote: ... Elution of RNA was done in nuclease-free water and 1 μl of rRNasin (Promega) was added before storage.
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bioRxiv - Cell Biology 2020Quote: ... Cells were lysed 48 hr post-transfection using 1 × Passive Lysis buffer (Promega, Madison, USA). Firefly and Renilla luciferase activities were measured using the Dual Luciferase Reporter assay system (Promega ...
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bioRxiv - Systems Biology 2019Quote: ... 1% Triton® X-100 0.1% Na deoxycholate) supplemented with protease inhibitor cocktail (Promega, G6521). Cells were lysed by mechanically means by passaging of cells through 26-gauge needle 5 times ...
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bioRxiv - Neuroscience 2019Quote: ... The mixture was fourfold diluted with ammonium bicarbonate and digested with trypsin (1:100; Promega) at 37 °C ...
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bioRxiv - Pathology 2020Quote: 0.2 µL GoScriptTM RT Mix for 1-Step RT-qPCR (Promega, Cat # A6120 and A6121)
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bioRxiv - Immunology 2021Quote: Viral loads were quantified using the GoTaq® Probe 1-Step RT-qPCR System (Promega). For quantification of SARS-COV-2 the nCOV_N1 primer/probe mix from the SARS-CoV-2 (2019-nCoV ...
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bioRxiv - Neuroscience 2020Quote: ... followed by secondary antibodies conjugated with HRP or alkaline phosphatase (Promega, Madison, WI, 1:1,000). Proteins were visualized with a LAS-3000 mini (GE Healthcare ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 μg of purified RNA was dissolved with 0.5 μg of oligo (dT) primer (Promega), 0.5 mM dNTPs ...
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bioRxiv - Biophysics 2021Quote: ... Cells were then incubated overnight at 4 °C with anti-p75NTR (Promega; G323A; 1:300) and anti-TRADD (Santa Cruz ...
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bioRxiv - Biochemistry 2021Quote: ... The reaction was started by addition of 1:4000 NanoGlo substrate (Promega, catalogue number N1110). The reaction was incubated for 5 minutes and the emission was measured at wavelengths of 445-470 nm and 520-545 nm using Tecan Spark multimode plate reader with luminescence readout and a settle time of 10 ms and integration time of 500 ms ...
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bioRxiv - Biophysics 2021Quote: ... followed by anti-rabbit alkaline phosphatase-conjugated secondary antibody (Promega, S3731, 1:5,000 in TBST) at room temperature for 1 h ...
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bioRxiv - Microbiology 2021Quote: Viral loads were quantified using the GoTaq® Probe 1-Step RT-qPCR System (Promega). For quantification of SARS-COV-2 the nCOV_N1 primer/probe mix from the SARS-CoV-2 (2019-nCoV ...
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bioRxiv - Cell Biology 2021Quote: ... 1 μg of RNA was used for cDNA synthesis using M-MLV reverse transcriptase (Promega). Primer sequences and amplicon sizes are listed in supplementary table 1 ...
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bioRxiv - Biochemistry 2020Quote: ... and mixed with the nanoluciferase substrate Nano-Glo (Promega cat# N1120, final dilution 1:200) for 2 min before measuring luminescence signals in a POLARstar OMEGA plate reader (BMG Labtech ...
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bioRxiv - Bioengineering 2021Quote: ... and 1 and 2 μL of 50 ng/μL trypsin (in 100 mM TEABC, Promega) was added to the wells with single cells and two hundred carrier cells ...
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bioRxiv - Microbiology 2021Quote: ... Secondary antibody labeling was completed using anti-rabbit IgG-HRP conjugate (1:10,000; Promega W401B) or anti-mouse IgG-HRP conjugate (1:10,000 ...
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bioRxiv - Microbiology 2021Quote: ... and an empty vector up to 1 μg of total DNA using FuGENE 6 (Promega). Sixteen hours later ...
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bioRxiv - Microbiology 2021Quote: ... cDNAs were then obtained from 1 μg of total RNA using AMV reverse transcriptase (Promega) and used directly for quantitative PCR (qPCR ...
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bioRxiv - Plant Biology 2021Quote: ... 1 µg total RNA was used to synthesize cDNA using M-MLV Reverse Transcriptase (Promega) and Oligo d(T)18 ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were splited After that cells were lysed with 1 X Passive Lysis Buffer (Promega). Renilla (RL ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 10% FBS and 1:1000 Nano-Glo® Luciferase Assay Substrate (Furimazine, Promega). First ...
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bioRxiv - Physiology 2022Quote: ... followed by 1:2500 goat anti-rabbit HRP-conjugated secondary (catalog #: W4011; Promega, Madison, WI). p-ATP1A1Y10 protein bands were visualized with SuperSignal™ West Pico Plus (SuperSignal Pico ...
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bioRxiv - Microbiology 2022Quote: ... Viral RNA was quantified by real-time RT-qPCR (GoTaq 1-step qRt-PCR, Promega) using 3.8μL of extracted RNA and 6.2μL of RT-qPCR mix and standard fast cycling parameters ...
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bioRxiv - Molecular Biology 2022Quote: ... the samples were incubated with a trypsin/lys-C mixture (w:w = 1:50; Promega, USA) at 37°C for 16 h and then the digest reaction was quenched with 1% formic acid (Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... Digestion of each sample was performed using 1 μg of Sequencing Grade Modified Trypsin (Promega) in TEAB per sample at 47 °C for 1 h ...
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bioRxiv - Cell Biology 2021Quote: ... followed by a 1:20,000 dilution of anti-mouse IgG HRP conjugated secondary antibody (Promega) for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... a total of 200 ng plasmid DNAs including (1) pGL4.53 vector encoding Fluc gene (Promega), (2 ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 μl of the resulting mRNA was mixed with 1 μl of FluoroTect GreenLys (Promega) and 3 μl of the wheat germ extract and then transferred under 50 μl of the translation buffer (1x SUB-AMIX SGC ...
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bioRxiv - Microbiology 2021Quote: ... Viral RNA was quantified by real-time RT-qPCR (GoTaq 1-step qRt-PCR, Promega) using 3.8µL of extracted RNA and 6.2µL of RT-qPCR mix and standard fast cycling parameters ...
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bioRxiv - Cell Biology 2021Quote: ... Neurons were then incubated overnight at 4° C with anti-p75NTR (Promega; G323A; 1:500) and anti-TRAF6 (Santa Cruz ...
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bioRxiv - Microbiology 2021Quote: ... 4°C) and then resuspended in 100 µl 1 x Passive Lysis Buffer (PLB, Promega). 45 µl of the luciferase assay substrate dissolved in Luciferase Assay Buffer II was plated on a 96-well plate and 10 µl of the cell lysate was added immediately before measurement of Fluc activity using a Tecan reader ...
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bioRxiv - Immunology 2020Quote: ... and cDNA synthesized from 1 µg total RNA (High Capacity cDNA Reverse Transcription Kit, Promega). Quantitative RT-PCR was performed using FluoCycle II SYBR Master Mix (EuroClone ...
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bioRxiv - Microbiology 2022Quote: ... Viral RNA was quantified by real-time RT-qPCR (GoTaq 1-step qRt-PCR, Promega) using 3.8 µL of extracted RNA and 6.2 µL of RT-qPCR mix and standard fast cycling parameters ...
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bioRxiv - Molecular Biology 2022Quote: ... terminated by incubation on ice and diluted 1:5 in Glo Lysis Buffer (Promega # E2661). 25 μL of prepared Nano-Glo reagent (Promega # N1120 ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 μL 10xcDNA and 0.3 μL of Forward and Reverse primers as recommended by Promega Corporation Super Real Premix ...
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bioRxiv - Neuroscience 2019Quote: Single cell PCRs were performed on diluted cDNA (1:100) using Go-Taq polymerase (Promega) with the following protocol ...
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bioRxiv - Microbiology 2019Quote: ... with primers specified in Supplementary Table 1 and cloned into vector pGEM-T Easy (Promega). A number of resulting clones was Sanger-sequenced and analyzed using the BLAST tool at PlasmoDB (www.plasmodb.org) ...
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bioRxiv - Plant Biology 2020Quote: 1 µg of input RNA was converted to cDNA using GoScript Reverse Transcriptase (Promega A501C) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: On bead digestion was performed by adding 1 μg of MS-grade trypsin (Promega, V5280) to each tube and incubating at 37 °C with shaking overnight ...
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bioRxiv - Microbiology 2020Quote: ... and lysed for 1 h in room temperature using 50 μl NanoLUC lysis buffer (Promega). NanoLUC enzymatic activity was measured using the NanoLUC substrate (Promega) ...
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bioRxiv - Microbiology 2021Quote: ... and lysed for 1 h in room temperature using 50 μl NanoLuc lysis buffer (Promega). NanoLuc enzymatic activity was measured using the NanoLuc substrate (Promega) ...
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bioRxiv - Cell Biology 2021Quote: Biotinylated sAB-K29 (100 µg) was incubated with 1 mL streptavidin-magnetic beads (Promega, Z5482) prewashed three times with PBS buffer in a thermo shaker for 3 h at 25 °C ...
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bioRxiv - Neuroscience 2021Quote: ... Neurite outgrowth was visualized by immunostaining with mouse anti-β III tubulin (1:1000; Promega) followed by incubation with Alexa 594-conjugated anti-mouse secondary antibodies (Invitrogen).
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bioRxiv - Developmental Biology 2020Quote: ... Primary antibody was incubated in 1× PBST (PBS + 0.1% Triton X-100) + 0.2% BSA (Promega) overnight at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were then diluted to 1 M urea with water and digested with trypsin (Promega) overnight at 37°C at a ratio of 1 μg of trypsin per 10 μg of protein for homogenate and cytosol samples while for light membranes ...
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bioRxiv - Molecular Biology 2020Quote: ... 2.5ng ml-1 recombinant human fibroblast growth factor (rhFGF) (Promega, Cat#G5071, Madison, WI, USA), and 1% chicken embryonic extract (Seralab ...
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bioRxiv - Immunology 2020Quote: ... RT-qPCR technique was performed according to GoTaq®1-Step RT-qPCR (Promega, USA) kit instructions ...
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bioRxiv - Genomics 2021Quote: ... cDNA was synthesised from 1 μg of total RNA using random primers (Promega, Wisconsin, USA) then treated with M-MLV Reverse Transcriptase (Pro-mega ...
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Role of the Topoisomerase IIα Chromatin Tether domain in Nucleosome Binding & Chromosome SegregationbioRxiv - Cell Biology 2021Quote: ... DLD-1 cells were transfected with the guide and donor plasmids using Viafect (#E4981, Promega) reagent ...