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Citations for Promega :
2601 - 2650 of 5642 citations for ssc mir 376a 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... were generated by digesting UPF1-GFP iso2 plasmid with BsrGI and XbaI (both sites located at the very end of GFP) and purifying it by Wizard® SV Gel and PCR Clean-Up System (Promega). Annealed oligonucleotides encoding the NLS of SV40 or NES of snurportin-1 with a stop codon and BsrGI and XbaI overhangs at the end are ligated into the linearised UPF1-GFP iso2 vector (oligonucleotides listed in Table S1) ...
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bioRxiv - Bioengineering 2022Quote: ... the mCherry coding sequence was PCR amplified from the plasmid pGFP-bait-MCS-NTR-mCherry using GoTaq® DNA Polymerase (Promega) with primers listed in Supplementary Table S3 ...
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bioRxiv - Cancer Biology 2022Quote: ... The promoter and enhancer candidate regions of ROR1 were amplified by PCR using KOD -Plus- Neo (Toyobo, KOD-401) and subcloned into the pGL3 luciferase reporter vector (Promega, E1751) (Figure 6C) ...
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bioRxiv - Synthetic Biology 2022Quote: ... and the integration of antibiotic resistance cassettes or reporter genetic constructs in the genome was confirmed by PCR under standard conditions using the GoTaq Green Master Mix (Promega, USA). The strains used and generated herein are shown in Table S1 ...
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bioRxiv - Microbiology 2022Quote: ... the PCR was performed in a total volume of 25 μl containing 5 X GoTaq® buffer (Promega, Madison, WI, USA), 2.5 mM MgCl2 ...
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bioRxiv - Physiology 2022Quote: The prepared PCR fragments were inserted into the pGEM-T vector via T/A cloning following the manufacturer’s recommendations (Promega, Fitchburg, USA). Competent Escherichia coli were transfected with the ligation mix and plated on agar plates as described previously [13] ...
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bioRxiv - Immunology 2022Quote: ... DDX60 (Fw 5’- AAGGTGTTCCTTGATGATCTCC-3’ Rv : 5’ -TGACAATGGGAGTTGATATTCC-3’) as analyzed by semiquantitative PCR using the SYBR Green assay GoTaq® qPCR Master Mix (Promega) with standardized primers (Metabion) ...
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bioRxiv - Microbiology 2022Quote: ... The DNA gel bands were excised and purified with a Wizard® SV Gel and PCR Clean-Up System (Promega, WI). The final amplicon products were quantified using the Qubit dsDNA HS Assay kit (Invitrogen) ...
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bioRxiv - Biochemistry 2023Quote: ... for either WT or mutant were PCR amplified from zebrafish shoc2 complementary DNA (cDNA) and cloned into the pCS2+ vector (Promega, WI). The capped mRNAs were synthesized with the mMessage mMACHINE SP6 transcription kit (Thermofisher Scientific ...
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bioRxiv - Genetics 2024Quote: ... 500–800 nt amplicons were amplified from genomic DNA from individual insertion lines through single fly PCR using GoTaq green master mix (Promega M7122).
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bioRxiv - Neuroscience 2024Quote: ... One µL of lysate was then added to make 20µL PCR reactions with GoTaq G2 Green Master Mix (Promega, Cat# M7823) and 1µM forward and reverse primers (Table 1) ...
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bioRxiv - Genomics 2024Quote: ... 1x SYBR Green I] and calculated from a standard dilution series of a PCR product (quantified with QuantiFluor® dsDNA System, Promega with a DeNovix DS-11 spectrometer ...
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bioRxiv - Immunology 2024Quote: ... A total of 10 ng of cDNA was used for quantitative PCR in a total volume of 10 µl with GoTaq® qPCR Master Mix (Promega) and specific primers ...
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bioRxiv - Microbiology 2024Quote: ... 1522bp using primers 5’-AAGGTACCTGAGGCTGGAGAGATGGCC-3’ and 3’-TAAAAGCTTCACCGGACTGGGCTAGTTCAG-5’ were PCR amplified and cloned in promoterless PGL3 enhancer empty vector (Promega, E1771) at the upstream of luciferase gene ...
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bioRxiv - Microbiology 2024Quote: ... DNA was isolated from 1 ml concentrated supernatant using Wizard PCR DNA Purification Resin and Minicolumns (both Promega, Madison, WI, USA) as previously described [74] ...
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bioRxiv - Genetics 2023Quote: ... and cloned the PCR products into JM109 competent cells using pGEM®-T Easy Vector Systems (Promega Corporation, Madison, WI, USA). Transformed cells were amplified and Sanger sequenced at the Genomics Facility ...
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bioRxiv - Immunology 2023Quote: ... 25 ng of c-DNA was used per reaction in each well containing the 2X SYBR green PCR master mix (Promega, USA) along with appropriate primers ...
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bioRxiv - Molecular Biology 2023Quote: ... The segment sequences of circPLXNA2 and MDM4 3’UTR containing the putative gga-miR-12207-5P binding sequence were amplified by PCR reaction and then subcloned into the XhoI and SalI restriction sites in the pmirGLO dual-luciferase reporter vector (Promega, USA).
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bioRxiv - Molecular Biology 2022Quote: ... whereafter the resulting DNA fragments were separated by gel electrophoresis and the required 5821 bp band was purified using the Wizard® SV Gel and PCR Clean-Up System (Promega). In this linear form ...
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bioRxiv - Cell Biology 2023Quote: ... double digested Capn4 PCR product was ligated with double digested pCWX200 or pLexA using the LigaFAst Rapid DNA Ligation System (Promega, M8226). The constructs were transformed into E ...
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bioRxiv - Evolutionary Biology 2023Quote: ... PCRs were performed in a final volume of 25 µL with 12.5 µL of GoTaq Green Mastermix (Promega, Madison WI, USA), 9.5 µL of water ...
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bioRxiv - Immunology 2023Quote: ... 25 ng of c-DNA was used per reaction in each well containing the 2X SYBR green PCR master mix (Promega, USA) along with appropriate primers ...
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bioRxiv - Cell Biology 2023Quote: ... a 1839 bp region between two KpnI sites was amplified by PCR and subcloned into pGEM-T Easy (Promega, Madison, WI). This subclone was subjected to site-directed mutagenesis using the Q5 site-directed mutagenesis kit (New England Biolabs ...
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bioRxiv - Cancer Biology 2023Quote: ... The A-tailing PCR products from the candidate clones were cloned into the pGEM-T vector (Promega, Madison, USA, Cat#A3600) and sequenced ...
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bioRxiv - Cancer Biology 2023Quote: ... All cells were confirmed to be free of mycoplasma (PCR-based method) and authenticated by Short Tandem Repeat (STR) analysis using the GenePrint® 10 System (Promega, Madison ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were stably transduced with a lentivirus-based expression construct generated by cloning a polymerase chain reaction (PCR)-amplified DNA fragment encoding luciferase from a pGL4.10-lucIIplasmid (Promega, Sydney, Australia) into a pLenti CMV Hygro DEST vector (Addgene ...
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bioRxiv - Microbiology 2023Quote: Insertion sites in individual clones were mapped using a two-step nested arbitrary PCR approach using 2X GoTaq master mix (Promega M7122). The first reaction (1X GoTaq master mix ...
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bioRxiv - Plant Biology 2024Quote: Protein expression was carried out using the in vitro transcription and translation system (TnT™ T7 Quick for PCR DNA, Promega) and the EMSA reaction was carried out following manufacturer’s instruction (odyssey) ...
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bioRxiv - Cancer Biology 2024Quote: The target sites for each crRNA were PCR amplified from genomic DNA extracted from tumor-bearing lungs using GoTaq Green® Mastermix (Promega) and the following primer pairs:
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bioRxiv - Microbiology 2023Quote: ... Marker genes in isolated DNA were polymerase chain reaction (PCR)-amplified using GoTaq Master Mix (Cat. No. M5133, Promega, Madison, Wisconsin) and 515 F (5′-GTGCCAGCMGCCGCGGTAA-3′) ...
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bioRxiv - Cell Biology 2023Quote: ... The pCMV5D_HA-WDR44 plasmid was purchased from MRC PPU Reagents and Services (DU26855) and a pFN21A-Halo-KIAA0100 plasmid (which served as a PCR template) was purchased from Promega (FHC00016). GFP-Rab8 and GFP-Rab11 were kind gifts from Dr ...
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bioRxiv - Plant Biology 2024Quote: ... the bands of desired sizes were gel-purified using Promega Wizard® SV Gel and PCR Clean-Up System (Promega, USA). PCR fragments were assembled into the Universal Level 0 vector (pAGM9121 ...
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bioRxiv - Genetics 2024Quote: ... Resulting amplicons were purified by gel extraction using Wizard SV Gel and PCR Clean-Up System (Promega cat no. PR-A9281) then cloned into TOPO TA pcR2.1 (Invitrogen cat no ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 2 µl of the lysed samples were added to 25 µl PCR reactions with GoTaq Green mastermix (Promega, Cat. No. M7123). After amplification ...
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bioRxiv - Microbiology 2024Quote: ... DNA bands at ∼390bp were excised from gel and purified using Wizard™ SV Gel and PCR Cleanup System (Promega A9282) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... After AvrLm4-7 PCR amplification with external primers (Gautier et al., 2023) the PCR products were cloned into pGEM®-T Easy Vector System (PROMEGA) using standard protocols ...
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bioRxiv - Immunology 2024Quote: ... To identify both light chains we performed subcloning of the light chain PCR products into pGEM-T-Vector System (Promega, A3600). First ...
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bioRxiv - Microbiology 2024Quote: ... were pooled and library cleanup was performed using a Wizard SV Gel and PCR Clean- Up System A9282 (Promega, Madison, WI). The pooled library was submitted to the UW Madison Biotechnology Center (UW-Madison ...
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bioRxiv - Molecular Biology 2024Quote: PCRs for amplification of chromosomal termini were performed on tailed DNA in 20 µL using GoTaq® Long PCR master Mix (Promega). PCR mix included 10 µL of GoTaq® Long PCR master Mix ...
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bioRxiv - Genomics 2021Quote: ... DNA was quantified using both the Qubit dsDNA HS Assay Kit on the Qubit 3.0 Fluorometer and the Plexor HY DNA Quantification Kit (Promega Corporation, Madison, WI) prior to submission for microarray genotyping.
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bioRxiv - Biochemistry 2023Quote: The NAD/NADH-Glo™ Assay kit (#G9071, LOT: 0000469987) and the NADP/NADPH- Glo™ Assay kit (#G9081, LOT: 0000472344) were purchased from Promega. Vacor (Pyrinuron N- 13738 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell lines were authenticated using genomic DNA extracted with Puregene Gentra Kit and multiplexed using the Promega Geneprint Kit and multiplexed with a STR-based method (Promega Geneprint System). Samples were run on an Applied Biosystems 3130xl DNA analyser and the results analysed using the Applied Biosystems Genemapper v4.1 software ...
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bioRxiv - Biochemistry 2023Quote: ... The P450-Glo CYP1A1/1B1 assay kit and NADPH regeneration system and luciferase assay kit were purchased from Promega (Promega, Madison, WI) and utilized under the following conditions ...
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bioRxiv - Cancer Biology 2024Quote: ... DNA was extracted from FFPE blocks using either the Qiagen AllPrep® DNA/RNA FFPE kit for FFPE-derived samples or using the Maxwell® RSC FFPE Plus DNA Kit (Promega). For frozen blocks ...
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bioRxiv - Molecular Biology 2023Quote: ... the RNA/Protein Purification Plus kit (Norgen, Thorold, ON, Canada) or the Maxwell 16 LEV simplyRNA purification kit (Promega, Madison, WI, USA) according to the manufacturers’ instructions ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA was synthesized with the ImProm II Kit (Promega). qPCR was performed using the Brilliant III Ultra-Fast SYBR® Green QPCR Master mix reagents (Agilent Technologies ...
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bioRxiv - Immunology 2021Quote: ... cDNA was synthesized with a reverse-transcriptase kit (Promega). RNA of cells exposed to SARS-CoV-2 WT was isolated with the QIAamp Viral RNA Mini Kit (Qiagen ...
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bioRxiv - Microbiology 2019Quote: ... and LEV DNA kit (AS1290, Promega, Madison, WI, US). The filters with collected biomass in lysing matrix E tubes were incubated with 300μL of lysing buffer and 30μL of Proteinase K and incubated at 56°C ...
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bioRxiv - Evolutionary Biology 2021Quote: ... luciferase signal was quantified using the DualGlo kit (Promega). Each experiment was performed in triplicate with two biological replicates ...
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bioRxiv - Molecular Biology 2021Quote: ... the Bactiter-Glo microbial cell viability assay kit (Promega) was used ...