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Citations for Promega :
2501 - 2550 of 5313 citations for 6 Bromo 3 3H imidazol 4 ylmethylene 1 3 dihydro indol 2 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... The fluorescently labeled PCR products were digested by using the 4-bp cutter TaqI (Promega, Southampton, United Kingdom) as described by the manufacturers ...
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bioRxiv - Plant Biology 2023Quote: ... Transcripts of interest were amplified using designed primers (Supplementary Table 4) and cloned into pGEMT-Easy vector (Promega). Digoxigenin-labelled antisense and sense RNA probes were transcribed from T7 or SP6 promoter of pGEMT-Easy vector (Promega ...
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bioRxiv - Plant Biology 2023Quote: Total RNA was isolated from 4-day-old wild-type seedlings using a Maxwell RSC Plant Kit (Promega). cDNA was synthesized from total RNA (100 ng ...
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bioRxiv - Molecular Biology 2023Quote: ... Ligation of the digested pGEM-T easy vector and 5’ fragments was carried out overnight at 4 °C using 0.5 µl (1.5 U) of T4 DNA Ligase (Promega). pGEM-T easy vector containing the 5’ fragment (5’-pGEM-T ...
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bioRxiv - Genomics 2024Quote: ... (34) DNA template and unused depletion probes were removed through addition of 4 μL RNase-free DNAse (Promega) with 5 μL 10 mM CaCl2 in 30 μL Nuclease-free water and incubated at 37 °C for 30 min ...
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bioRxiv - Immunology 2021Quote: ... PCR was performed with the generated cDNA and premixed 2× Taq polymerase solution (Promega) in an MJ Mini Thermal Cycler (BioRad) ...
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bioRxiv - Genetics 2019Quote: ... total RNA was treated with 2 U of RQ1 RNase-Free DNase (Promega, USA) at 37°C for 30 min ...
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bioRxiv - Genomics 2021Quote: - A combination of KAPA HotStart ReadyMix and Pfu DNA Polymerase (2 U/μL, Promega) was used in some tests to try to increase enzyme processivity ...
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bioRxiv - Physiology 2021Quote: ... and simultaneously transfected with 2 μg of DNA using Fugene HD (catalog #E2312, Promega), accordingly to the manufacturer instructions ...
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bioRxiv - Microbiology 2022Quote: ... 2 cm strips of intestinal tissues were embedded in low melting point agarose (Promega) to enrich for well-oriented crypt-villus units ...
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bioRxiv - Plant Biology 2021Quote: ... http://cshprotocols.cshlp.org/content/2009/2/pdb.prot5110.abstract) with trypsin (porcine, side chain protected; Promega). Briefly ...
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bioRxiv - Biochemistry 2020Quote: ... and the pre-cleared lysate was supplemented with 2 µl of RNasin Plus (Promega), added onto the freshly prepared ...
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bioRxiv - Biophysics 2020Quote: ... containing 10% (vol/vol) FBS and 2% (vol/vol) GloSensor cAMP stock solution (Promega). The cells were then incubated for 2 hours at room temperature in the dark ...
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bioRxiv - Microbiology 2020Quote: ... Two days after transfection with 2 to 2.5 μg DNA using Fugene HD (Promega), confocal images were acquired using an FV1000 confocal laser scanning microscope (Olympus ...
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bioRxiv - Microbiology 2021Quote: ... We synthesized cDNA from 2 μL of RNA using M-MLV reverse transcriptase (Promega) and random hexamers ...
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TRANSITION OF PODOSOMES INTO ZIPPER-LIKE STRUCTURES IN MACROPHAGE-DERIVED MULTINUCLEATED GIANT CELLSbioRxiv - Cell Biology 2020Quote: ... PCR was performed with the generated cDNA and premixed 2× Taq polymerase solution (Promega) in an MJ Mini Thermal Cycler (BioRad) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Luminescence was initiated by addition of a mixture of 2 μM coelenterazine-h (Promega) and 40 μM 3-isobutyl-1-methylxanthine (IBMX ...
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bioRxiv - Microbiology 2020Quote: ... RNA (2 μg) was used for cDNA synthesis with M-MLV Reverse Transcriptase (Promega). Conventional PCR was used with the primer (Table 1 ...
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bioRxiv - Immunology 2021Quote: ... F(ab′)2 fragments were generated after cleavage of IgG with IdeS protease (Promega) and then purified using TALON metal affinity resin (Takara ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were transfected with 2 μg of the respective guideRNA plasmids using FuGene (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... with 2 uM Ponasterone A overnight and incubated with JF646 HaloTag ligand (Promega, GA1120) 15 minutes before imaging ...
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bioRxiv - Plant Biology 2023Quote: ... Two (2) μg of total RNA were used for cDNA synthesis with MMLV (Promega) and random hexamers (Invitrogen) ...
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bioRxiv - Biochemistry 2023Quote: ... or a combination of 2 μg of trypsin and 0.2 μg of LysC (Promega). Samples were incubated at 37 °C overnight ...
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bioRxiv - Microbiology 2024Quote: ... for 2 h at 25 °C and digested with trypsin (Promega, 37 °C, overnight) in TEAB buffer (50 mM ...
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bioRxiv - Cell Biology 2023Quote: ... and proteins were further digested by the addition of 2 μg trypsin (Promega #V5113) for 4 hours at room temperature on a shaker ...
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bioRxiv - Microbiology 2023Quote: ... and TCID50 equivalents determined using a SARS-CoV-2 E gene detection kit (Promega) based upon the Berlin primer/ probe set (70).
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bioRxiv - Molecular Biology 2023Quote: ... including NCI were spiked with 2 ng of Luciferase Control RNA (LUC) (Promega, USA). These extracts were used for real-time quantitative PCR (RT-qPCR ...
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bioRxiv - Neuroscience 2023Quote: ... cDNA was generated from 2 µg isolated RNA using M-MLV reverse transcriptase (Promega) and diluted 1:15 for use in 12 µL qPCR reactions run on the StepOnePlus™ system (Applied Biosystem) ...
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bioRxiv - Molecular Biology 2023Quote: ... purified RNAs were additionally incubated with 2 units of RQ1-DNAse (Promega, cat# M6101) per 1 µg of RNA at 37°C for 30 min to eliminate plasmid DNA contamination ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were digested for 18 h at 37 °C with 2 μg trypsin (Promega). After digestion ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 µg total RNA served as a template using M-MLV Reverse Transcriptase (Promega) and random hexamer primers following manufactureŕs protocol ...
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bioRxiv - Plant Biology 2024Quote: ... 2 mg of the extracted RNA underwent treatment with RQ1 RNase-Free DNAse (Promega) before being used for cDNA synthesis with SuperScript IV Reverse Transcriptase (Invitrogen) ...
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bioRxiv - Biochemistry 2023Quote: ... peptides were collected as described in ProteaseMax application note (Promega, TB373, Rev. 2/15), and purified using Pierce C18 Spin Tips (Thermo Fisher PI84850) ...
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bioRxiv - Molecular Biology 2023Quote: ... and 24 hours later co-transfected with 500 ng psiCHECK™-2 Vector (Promega) containing the 3’UTR of human PLIN2 gene downstream to the Renilla luciferase gene ...
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bioRxiv - Cell Biology 2024Quote: ... for 20ms with 2 pulses or lipid-mediated delivery using FuGENE HD (Promega; TM328) according to the manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2024Quote: ... HaloTagged AP-2 σ2 was visualized by adding Janelia Fluor 646-HaloTag ligand (Promega). Ligand (100 nM ...
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bioRxiv - Immunology 2021Quote: ... A 1:1 dilution of Steady Glo (Promega) and Lysis Buffer were added to the cells and incubated at RT for 15 mins ...
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bioRxiv - Cell Biology 2023Quote: ... mouse anti-1-gal (1:1000, Promega Z378B), mouse anti-1-gal (1:1000 ...
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Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelopebioRxiv - Cell Biology 2023Quote: ... 1 mM CaCl2 with 1 µg trypsin (Promega). Digested proteins were acidified with TFA to pH < 2 and RapiGest SF was precipitated out ...
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bioRxiv - Systems Biology 2019Quote: ... and 20 μL of diluted insect cell extract (containing 4 μL of ICE, 3.2 μL of 5x ICB dilution buffer and 8 units of T7-RNAP-Promega#P4074 ...
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bioRxiv - Immunology 2021Quote: ... Single CD3-CD8-CD14-CD16-CD20+Ova-RBD-PE+RBD-AF647+ B cells were sorted into individual wells of 96-well plates containing 4 μl of lysis buffer (0.5× PBS, 10 mM DTT, 3,000 units/ml RNasin Ribonuclease Inhibitors (Promega, N2615) per well using a FACS Aria III and FACSDiva software (Becton Dickinson ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were resuspended in 4 ml of Blob100 buffer plus 80 units/ml RNasin Plus (Promega, Flitchburg, WI, USA) and lysed under liquid nitrogen in a 6870 Freezer Mill (Spex ...
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bioRxiv - Cell Biology 2021Quote: ... collected at 2000 x g followed by resuspension in 0.5 ml of storage buffer (PBS containing 4% BSA and RNasin (Promega) – 0.2 U/ul ...
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bioRxiv - Plant Biology 2021Quote: ... On bead digestion was performed in 50 µl of ammonium bicarbonate solution containing 4 µl of Trypsin/LysC (Promega) solution and incubated at 37°C for 3 h in a thermomixer at 1,000 RPM ...
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bioRxiv - Immunology 2022Quote: ... Single CD3−CD8−CD14−CD16−CD20+Ova−Gamma NTD-PE+Wuhan-Hu-1 NTD-AF647+ B cells were sorted into individual wells of 96-well plates containing 4 μl of lysis buffer (0.5× PBS, 10 mM DTT, 3,000 units/ml RNasin Ribonuclease Inhibitors (Promega, N2615) per well using a FACS Aria III and FACSDiva software (Becton Dickinson ...
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bioRxiv - Cell Biology 2022Quote: ... 4 hours post-transfection media was replaced with fresh complete DMEM containing 646 HALO Dye (20 nM; GA112A; Promega). The next day ...
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bioRxiv - Developmental Biology 2019Quote: ... Ark -/- MEFs were transfected with 4 guide pairs in order to create the desired deletion using FUGENE (Promega, E2311) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... or day 4 in TZM-bl cells by measuring luciferase activity using the luciferase assay system (Promega, Madison, WI) and the microplate luminometer Monolight 3096 (BD Biosciences ...
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bioRxiv - Plant Biology 2020Quote: ... template cDNA samples were prepared using 4 κg of total RNA and the Improm-II reverse transcription system (Promega). Primers were designed to have a Tm of ~60°C and to produce PCR products of ~200−400 base pairs ...
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bioRxiv - Cancer Biology 2019Quote: ... Proteins were pelleted at 20,000 × g and washed with ice cold 10 mM HCl / 90% acetone before resuspension in 60 μL 4 M Urea and 0.5 × ProteaseMax (Promega), vortexed ...