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Citations for Promega :
2301 - 2350 of 5313 citations for 6 Bromo 3 3H imidazol 4 ylmethylene 1 3 dihydro indol 2 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Figure 6 and Figure 7) to form condensates in cells was monitored by transient transfection of U-2 OS cells using FuGene (Promega; Figures 2 and 7) or electroporation (as before ...
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bioRxiv - Cell Biology 2020Quote: ... following manufacturer’s instructions using 200 ng psiCHECK™ 2 (Promega) as empty vector control or psiCHECK2 containing Tnf-α 3’ UTR or Il6 3’ UTR and 30 pmol miRNA scramble negative control ...
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bioRxiv - Molecular Biology 2019Quote: A commercial kit (HDAC-Glo™ 2 Assays, Promega, G9590) was used to measure HDAC2 activity ...
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A general role of zinc binding domain revealed by structures of σ28-dependent transcribing complexesbioRxiv - Molecular Biology 2020Quote: ... samples were treated with 2 U/mL DNase I (Promega) for 1 min at 37 °C ...
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bioRxiv - Biochemistry 2020Quote: ... After adding 2 μl of trypsin (0.5 μg/μl, Promega), peptide mixtures were incubated at 37°C for 4 hrs ...
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bioRxiv - Biochemistry 2020Quote: ... Before adding 2 μl of trypsin (0.05 μg/μl, Promega), 500 fmol of isotope-labeled peptides 1 and 2 were added to spike the ROS preparation ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 µg of total RNA was treated with DNAse (Promega) and utilized as a template for the cDNA synthesis reaction using MMLV reverse transcriptase (Promega ...
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bioRxiv - Biochemistry 2021Quote: ... Amplified DNAs were cloned into pSiCHECK-2 (Promega, Wuhan, China) by utilizing XhoI and NotI restriction enzyme sites to construct pSi2-hUTR and pSi2-mUTR ...
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bioRxiv - Neuroscience 2020Quote: ... using the GoTaq® 2-step RT-qPCR kit (Promega). Quantitative PCR runs were performed on a 7900HT Fast Real-Time PCR System (Applied Biosystems Inc. ...
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bioRxiv - Biochemistry 2020Quote: ... T7 RNA polymerase and 2 µL RNasin RNase inhibitor (Promega) and incubated overnight at 37°C ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Polymerase chain reactions were performed using 2 U Gotaq (Promega), 1X Gotaq buffer (Promega) ...
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bioRxiv - Microbiology 2021Quote: ... 2 μg of 0.5 μg/μl sequencing grade trypsin (Promega) was added ...
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bioRxiv - Microbiology 2021Quote: To clone the 3xant-miR-275 into psiCheck-2 (Promega), two complementary single-stranded oligos that encode 3xant-miR-275 and XhoI and NotI restriction endonuclease cut sites were synthesized at Yale Keck Oligo Synthesis Resource (Table 1) ...
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bioRxiv - Cell Biology 2022Quote: ... the protein suspension was digested with 2 µg trypsin (Promega) overnight at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were transfected with psiCHECK-2 plasmids (Promega, Madison, USA) containing two luciferase genes ...
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bioRxiv - Cell Biology 2024Quote: ... the protein suspension was digested with 2 µg trypsin (Promega) overnight at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... psiCHECK2-M was a modified form of psiCHECK-2 (Promega) with deletion of the HSV-TK promoter (Figureure 5A) ...
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bioRxiv - Cancer Biology 2023Quote: ... MTS reagent (2 mg/ml stock; Cat. No. G1111, Promega) was freshly supplemented with phenazine methosulfate (PMS ...
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bioRxiv - Microbiology 2023Quote: ... and 2× GoTaq qPCR Master Mix (Promega, Madison, WI, USA). RT-qPCR was performed using the MyGo Pro real-time PCR instrument (IT-IS Life Science Ltd. ...
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bioRxiv - Cell Biology 2023Quote: ... 2 µL of 50% Cell-Titer Glo (Promega cat G7573) diluted in water was added to the cells and luminescence measured on an EnVision Plate Reader (PerkinElmer).
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bioRxiv - Molecular Biology 2022Quote: ... and 2% of beetle luciferin sodium salt (Promega, Madison, USA). For luminescence measurements ...
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bioRxiv - Cancer Biology 2023Quote: ... cell viability was measured using CellTiter-Glo 2 reagent (Promega). After addition of the reagent to cell culture medium ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell viability was detected using CellTiter Glo 2 reagent (Promega). After addition of the reagent to cell culture medium ...
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bioRxiv - Neuroscience 2023Quote: ... 2) an unrelated male fibroblast genomic DNA sample (Promega, G1471), and 3 ...
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bioRxiv - Molecular Biology 2023Quote: ... viability was measured using CellTiter-Blue assay (G8081/2, Promega) following the protocol of the manufacturer ...
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bioRxiv - Molecular Biology 2023Quote: ... and GTaq® qPCR Master Mix (Promega, Cat.# A6001/2) were utilized.
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bioRxiv - Plant Biology 2023Quote: ... 2 μg of total RNA treated with DNase RQ1 (PROMEGA) was reverse transcribed with Superscript IV (Invitrogen ...
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bioRxiv - Systems Biology 2024Quote: ... before adding 2 µL of 0.5 ng/µL trypsin (Promega) in digestion buffer (50 mM TEAB ...
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bioRxiv - Cell Biology 2024Quote: ... 2 μL of 50% Cell-Titer Glo (Promega No G7573) diluted in water is added to the cells and luminescence measured on an EnVision Plate Reader (Perkin Elmer)
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bioRxiv - Microbiology 2020Quote: ... Transfection of HEK293T cells was performed at 80% confluence using 25ng of each plasmid construction mixed with 150nL of Fugene 6 (E2691, Promega, France) reagent per well ...
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bioRxiv - Cell Biology 2020Quote: ... gel regions were diced and proteins were subjected to in-gel digestion with 6 ng/μL trypsin (Promega, Madison, WI, USA) in 50 mM ammonium bicarbonate at 37 °C for 10-12 hours ...
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bioRxiv - Genomics 2019Quote: ... pSMGV Dam-V5-Lap2β (Dam-Lap2β) and pSMGV Dam-V5-EMD (Dam-EMD) were transfected using Fugene 6 transfection reagent (Promega, E2691) into the Platinum-E packaging line (Cell Biolabs ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were digested overnight at RT with a 1:50 ratio of sequencing grade modified trypsin (0.4 μg for whole proteome samples, 6 μg for phospho samples, V511A, Promega, Fitchburg WI) in 130 μL TEAB (0.05M Triethylammoniumbicarbonate in water) ...
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bioRxiv - Cancer Biology 2022Quote: ... Media was refreshed on day 6 and plates collected on day 12 with cell viability quantified by CellTiter-Glo 3D (Promega #G9681). Dose response assay analysis was performed as described above.
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bioRxiv - Molecular Biology 2021Quote: ... and cells with approximately 70-90% confluency in each plate were transfected with required plasmid(s) for each assay using Fugene 6 transfection reagent (Promega, USA) or 1 mg/ml PEI (polyethyleneimine).
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bioRxiv - Microbiology 2020Quote: Plasmids encoding each vector were transfected into HEK293 cells seeded in 10 cm plates using Fugene 6™ (Promega, Madison, WI) or polyethyleneimine ...
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bioRxiv - Cell Biology 2019Quote: ... a puromycin resistance marker and specific gRNA guides against VPS35, SNX5 or SNX6 (Kvainickas et al., 2017; Simonetti et al., 2017) using FuGENE® 6 (Promega). The day after transfection ...
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bioRxiv - Cancer Biology 2020Quote: ... used for infection of p53-null NSCs were obtained from previously transfected DF1 chicken fibroblasts (ATCC #CRL-12203) using FuGENE 6 Transfection reagent (Promega #E2691), according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... the cells were transiently transfected with pUC57 constructs harboring 1.3-mer HBV genomes (genotypes A2, B2, C2, and D3) using FuGene 6 transfection reagent according to the manufacturer’s instructions (Promega, WI, USA). The generation of plasmids has been previously described and this transient expression system relies on the endogenous promoters of HBV for transcription (6) ...
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bioRxiv - Molecular Biology 2019Quote: ... Transient transfections were performed in 24-well plates using 500ng of dCas9 expression vector and 250ng of equimolar pooled or individual sgRNA expression vectors mixed with FuGENE® 6 (Promega) following manufacturer’s instructions.
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bioRxiv - Molecular Biology 2021Quote: XAB2-GFP and RNAP2-GFP stably expressing cell lines were produced by transfecting XAB2-GFP or RNAP2-GFP using FuGENE® 6 Transfection Reagent (Promega) according to the manufacturer’ protocol ...
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bioRxiv - Immunology 2021Quote: Pseudoviruses expressing complete SARS-CoV2 spike genes were prepared by transient transfection of HEK293T cells with three plasmids: SARS-CoV2 MLV-gag/pol and MLV-CMV-luciferase plasmids using Fugene 6 (Promega Inc.) as described earlier (Rogers et al. ...
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bioRxiv - Microbiology 2022Quote: ... The effector cells and serum were incubated with the cells for 6 hours after which 75ul Bio-Glo reagent (Promega; G7940) was added to each well ...
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bioRxiv - Microbiology 2024Quote: ... and simultaneously transfected with 0.4 μg of respective EGFP-Rab plasmids using FuGENE 6 transfection reagent (Cat. E269A, Promega, Madison, WI) per the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... and gene expression was determined by quantitative PCR (QuantStudio 6 Pro Real-Time PCR System) with GoTag qPCR System (Promega, A6002). Actb and Rplp0 were used as housekeeping genes ...
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bioRxiv - Immunology 2023Quote: ... cells were transfected with 12 µg of plasmid expressing the spike from various variants using Fugene-6 (Promega; catalog number: E2691) according to the manufacturer’s instructions and incubated at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... or pCEP4-OCT4-T234A-S235A (addgene, 40630) or pCEP4-OCT4-T234E-S235E (addgene, 40631) for 48 h using FuGENE 6 (Promega, E2691) according to the manufacturer’s protocol.
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bioRxiv - Physiology 2024Quote: ... a targeting vector was constructed by subcloning a 9.1 kb region from an Akr1b7 positively identified C57BL/6 fosmid clone (WI1-1577M19) into the 2.45 kb-iTL cloning vector derived from the pSP72 vector (Promega; Madison, WI). The region was designed so the long homology arm (LA ...
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bioRxiv - Microbiology 2024Quote: ... 4 μg of each of the 3 HIV-envelope expression plasmids were co-transfected with 8 μg of backbone plasmid pSG3ΔEnv in HEK 293T cells using FuGene 6 transfection reagent (Promega, catalog # E5911). Virus was harvested 48 h post-transfection ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... after which growth media was removed and 20 µl of 4% D-luciferin (Promega) in CO2 independent medium (Thermo Fisher Scientific ...