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Citations for Promega :
201 - 250 of 3876 citations for Urea Nitrogen Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... The proteins were rinsed with urea and ammonium bicarbonate solutions and digested overnight at 37°C using trypsin gold (Promega) at a ratio of 1:100 ...
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bioRxiv - Biophysics 2022Quote: ... Then the samples were diluted to 1 M urea with 50 mM (NH4)HCO3 (AB) and trypsinized (1:50 m/m, Promega) at 37 °C (overnight ...
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bioRxiv - Neuroscience 2023Quote: ... The samples were then diluted with 50 mM NH4HCO3 to a final concentration of less than 2M urea and then and further digested overnight with 1:50 (w/w) trypsin (Promega) at 25°C ...
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bioRxiv - Neuroscience 2023Quote: ... The samples were then diluted with 50 mM NH4HCO3 to a final concentration of less than 2M urea and then and further digested overnight with 1:50 (w/w) trypsin (Promega) at 25°C ...
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bioRxiv - Cell Biology 2022Quote: ... diluted by 4-fold to reduce urea to 2 M for the addition of trypsin (Promega, 1:50 w/w) to continue the digestion at 21°C overnight ...
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bioRxiv - Pathology 2024Quote: ... The samples were diluted with 50 mM TEAB buffer to a final Urea concentration of 2 M before adding trypsin (Promega) in an enzyme:substrate ratio of 1:75 and incubating at 25 °C for 16 hours ...
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bioRxiv - Neuroscience 2024Quote: ... Afterwards the samples were diluted to decrease the concentration of urea up to 1 M and supplemented with additional amounts of sequencing-grade trypsin (Promega) (200 ng for pull-down samples and 1 ug for total lysate samples) ...
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bioRxiv - Neuroscience 2024Quote: ... diluted by 4-fold to reduce urea to 2 M for the addition of trypsin (Promega, 1:50 w/w) to continue the digestion at 21ºC overnight ...
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bioRxiv - Neuroscience 2023Quote: ... samples were diluted with 50 mM NH4HCO3 to a final concentration of less than 2 M urea and were further digested overnight with 1:25 (w/w) trypsin (Promega) at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: Proteins bound to beads were reduced with 2 mM DTT in 2 M urea buffer and sequencing grade trypsin (Promega) was added to a final concentration of 5 ng/μl ...
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bioRxiv - Microbiology 2023Quote: ... to a final urea concentration ˂ 2 M and then digested with modified trypsin (1:50 w/w) (Sequencing Grade, Promega) at 37°C for 3 h ...
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bioRxiv - Developmental Biology 2023Quote: Protein extracted from 10 CNC explants as described above or immunoprecipitated with the Flag antibody from embryos or Hek293T cells were diluted in 8M urea and processed with Trypsin/Lysine-C according to manufacturer instruction (Promega). Tandem mass spectrometry was performed using a Thermo orbitrap Fusion (Mass spectral data were obtained at the University of Massachusetts Mass Spectrometry Core Facility ...
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bioRxiv - Biochemistry 2023Quote: ... proteins were diluted with 50 mM NH4HCO3 to a final concentration of 2 M urea and digested with trypsin (Promega), at an enzyme-to-substrate ratio of 1:20 ...
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bioRxiv - Biochemistry 2023Quote: ... then washed and resuspended in 2 M urea and trypsinized overnight with 0.5 μg/μl sequencing grade trypsin (Promega, V5111). Tryptic peptides were eluted off with spin columns (BioRad ...
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bioRxiv - Plant Biology 2024Quote: ... The sample solutions were diluted to a final concentration of 2 M urea and 50 mM ammonium bicarbonate prior to the trypsin (Promega) digestion ...
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bioRxiv - Molecular Biology 2024Quote: ... diluted by 4-fold to reduce urea to 2 M for the addition of trypsin (Promega, 1:50 w/w) to continue the digestion at 21 °C overnight ...
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bioRxiv - Biochemistry 2024Quote: ... elution buffer I (2 M Urea, 50 mM Tris pH 7.5, 1 mM DTT and 5 ng/µl Trypsin (Promega, #V5111)) were added and samples were incubated for 1 h at RT ...
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bioRxiv - Molecular Biology 2024Quote: ... 30 min) and diluted to 1M urea with 200 mM ammonium bicarbonate for trypsin digestion (1 µg, 37°C, 8h, Promega). After digestion ...
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bioRxiv - Genetics 2021Quote: ... Development and stop solutions were added followed by detection of absorbance at 450 on a Luminometer: GloMax-Multi Detection System (Promega, USA).
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bioRxiv - Neuroscience 2020Quote: The toxicity of PX was investigated on monolayers of BLEC seeded on 96 well plates or on inserts luminal side in co-culture experiments using the commercially available Cytotoxicity Detection Kit (Promega, United States). An aliquot of 25 μl medium was taken to quantify the lactate dehydrogenase (LDH ...
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bioRxiv - Cell Biology 2021Quote: TdT-mediated dUTP nick-end labeling (TUNEL) or EdU assays were performed by using in situ cell death detection kit (TUNEL, Promega Corporation, G3250) or EdU Click-iT® Assay Kit obtained from Thermo Fisher (C10639) ...
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bioRxiv - Physiology 2020Quote: ... The transferase dUTP Nick End Labeling (TUNEL) assay was performed on cryosections of rat eyes using the TUNEL apoptosis detection kit (DeadEnd Fluorometric TUNEL System; Promega, Madison, WI) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: The ATP quantification was performed using ENLITEN® ATP Assay System Bioluminiscence Detection Kit from Promega (protocol by (Yang et al., 2015)) ...
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bioRxiv - Immunology 2023Quote: DNA in the HHMX-stimulated supernatants of THP1 dual reporter cells was quantified using the QuantiFluor dsDNA Detection Kit from Promega (catalog # E2670) according to the manufacturer’s instructions.
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bioRxiv - Plant Biology 2024Quote: ... Rosette leaf samples were ground in liquid nitrogen and total RNA was extracted using the SV total RNA isolation system (Promega, www.promega.com). After quantification with a Nanodrop system (www.nanodrop.com) ...
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bioRxiv - Microbiology 2020Quote: ... using a GloMAX®-Multi Detection system (Promega, UK). 50 µL reagent added at a speed of 200 µl/s followed by mixing and 2 s delay ...
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bioRxiv - Microbiology 2022Quote: ... and read on a GloMax multi-detection system (Promega).
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bioRxiv - Bioengineering 2021Quote: ... for radiography detection or with untagged Methionine (Promega #L118A) and Green Lysine (FluoroTect™ GreenLys ...
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Core Microbiota Drive the Prevalence of Extracellular Antibiotic Resistome in the Water CompartmentsbioRxiv - Microbiology 2022Quote: ... Detection and quantification using QuantiFluor ™ -ST (Promega, USA). The raw eDNA samples and PCR products were checked by agarose gel electrophoresis (Fig ...
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bioRxiv - Neuroscience 2019Quote: ... ADP detection system (ADP-Glo) was purchased from Promega. Inhibitors were synthesized as described by Boyd et al [18] ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 100 μl ROS-Glo detection reagent (Promega, Southampton, UK) was added per well ...
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bioRxiv - Molecular Biology 2023Quote: ... 50ul of NAD/NADH-Glo™ Detection Reagent (Promega) was added to each well ...
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bioRxiv - Plant Biology 2023Quote: ... and quantified with a GloMax-Multi Detection System (Promega).
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bioRxiv - Molecular Biology 2023Quote: ... the Nano-Glo HiBiT Lytic Detection Assay (N3040, Promega) was performed according to the manufacturer’s guidelines ...
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bioRxiv - Bioengineering 2023Quote: ... and the GloMax®-Multi Detection luminometer System (Promega). The experiments were carried out in three biological replicates for the Z-Cre vs ZZ-Cre ZIKV replicase comparison experiment and once for the ZZ-Cre NIEV ...
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bioRxiv - Immunology 2023Quote: ... the luciferase substrate was added for chemiluminescence detection (Promega; Luciferase Assay system ...
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bioRxiv - Cell Biology 2023Quote: ... 2 μL of ONE-glo luciferase detection reagent (Promega) was dispensed per well and luminance signal recorded with an Envision plate reader (Perkin Elmer).
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bioRxiv - Immunology 2024Quote: ... Plates were read on GloMax Multi Detection System (Promega) at wavelengths of 480 nm and 520 nm for excitation and emission ...
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bioRxiv - Biochemistry 2020Quote: ... Digestion solutions were brought to a final concentration of 2 M urea and 2 mM CaCl2 and a second digestion was performed overnight at 37 °C using trypsin (Promega, v5280) at 1:100 w/w ...
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bioRxiv - Cell Biology 2021Quote: ... 30 min) and diluted to 1M urea with 200mM ammonium bicarbonate for trypsin digestion (1μg, 37°C, 8 h, Promega cat # V5113).
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bioRxiv - Microbiology 2021Quote: ... as described above was digested with chymotrypsin or trypsin/lysC using the Accumap™ low pH sample preparation with urea under nonreducing conditions (Promega). The free cysteines in the Mpro preparations (100 µg ...
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bioRxiv - Biochemistry 2021Quote: ... on-bead digestions were done with 2 µg LysC (Wako chemicals 125-05061) in 4 M urea and 4 µg trypsin (Promega ADV5113) in 1.2 M urea sequentially before quenching with 1% formic acid ...
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bioRxiv - Systems Biology 2022Quote: ... The urea concentration was then diluted to 1 M with 50 mM ABC and the proteins digested with trypsin (Promega, V511c) at 1:100 mass-ratio at 37 °C overnight.
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bioRxiv - Systems Biology 2019Quote: ... The samples were then diluted with 0.1 M NH4HCO3 to a final concentration of 1 M urea and the proteins were digested with sequencing-grade porcine trypsin (Promega, Switzerland) at a final enzyme:substrate ratio of 1:100 (w/w) ...
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bioRxiv - Neuroscience 2023Quote: ... was performed by incubating the proteins first with endoproteinase Lys-C (Wako, #125-05061,)) in 8 M urea for 4h at 37°C followed by an overnight trypsin digestion (Promega #V511A,) in 2 M urea at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... 30 min) and diluted to 1M Urea with 200 mM ABC for trypsin digestion (1 μg, 37°C, overnight shaking, Promega, #V5113). On the next day ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was then resuspended in 30 μL of 8M urea in PBS and 30 μL of ProteaseMax surfactant (20 μg/mL in 100 mM ammonium bicarbonate, Promega, V2071) with vortexing ...
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bioRxiv - Biochemistry 2023Quote: ... The reaction was quenched with DTT and urea was diluted to <1 M with 50 mM NH4HCO3 before adding MS-grade trypsin (Promega, V5113) in a ratio of 1:25 (trypsin:protein ...
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bioRxiv - Plant Biology 2023Quote: ... The final pellet was dried at room temperature and resuspended in 500 µL of [7 M urea (Promega, Madison, WI, USA), 2 M thiourea (Sigma-Aldrich Corp. ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was then resuspended in 30 μL of 8 M Urea and 30 μL of ProteaseMax surfactant (20 μg/mL in 100 mM ammonium bicarbonate, Promega, V2071).