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Citations for Promega :
151 - 200 of 3876 citations for Urea Nitrogen Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... before being washed and resuspended in 2 M urea and trypsinized overnight with 0.5 μg μl−1 sequencing grade trypsin (Promega). Tryptic peptides were eluted off ...
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bioRxiv - Biochemistry 2019Quote: ... All samples were further diluted with 20 mM HEPES pH 8.0 to a final urea concentration of 2 M and proteins were digested with 5 μg trypsin (V5111, Promega) (1/100 ...
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bioRxiv - Cancer Biology 2019Quote: ... Proteins were pelleted at 20,000 × g and washed with ice cold 10 mM HCl / 90% acetone before resuspension in 60 μL 4 M Urea and 0.5 × ProteaseMax (Promega), vortexed ...
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bioRxiv - Immunology 2020Quote: ... Samples were then diluted with 50 mM HEPBS pH 8.3 to reduce urea concentration down to 1 M and digested with trypsin (Promega) at 1:10 enzyme:substrate ratio overnight at 37°C ...
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bioRxiv - Biochemistry 2020Quote: ... for 4 h at 37°C and then the urea concentration was reduced to 1.5 M and digested was finalized with 1:50 (w/w) trypsin (Promega) overnight at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Precipitates were solubilised in 8M urea (20mM HEPES, pH 8) and digested with Lysine C (1:50 ratio; Promega) for 4h at 37C in 6M urea followed by an overnight trypsin digestion (1:25 ratio ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were diluted with 100 mM TEAB until containing 0.6 M urea and digested with sequencing grade modified trypsin (Promega) using a 1:150 enzyme-to-substrate ratio at 37°C for 16 hours ...
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bioRxiv - Microbiology 2022Quote: ... 10 μg of protein were digested overnight at 37°C in 2 or 1.5 M urea (for BB22OP and RIMD 221063 samples, respectively) and 25 mM ammonium bicarbonate with modified trypsin (Promega), in a 1:50 (M/M ...
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bioRxiv - Molecular Biology 2023Quote: ... and then diluted to 1M urea with 200 mM ABC for trypsin digestion (Sequence-grade, #V5111, Promega, WI, US) (1 µg ...
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bioRxiv - Evolutionary Biology 2022Quote: ... we dissolved 10 μg of protein in 10 μl 10 M urea containing 0.1% ProteasMax (Promega. Madison, WI, USA). Cysteine residues were reduced with 5 mM DTT (30 min at 50 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... precipitated proteins were eluted by incubation of beads with Elution Buffer (2 M Urea, 50 mM Tris pH 7.5, 2 mM DTT, 20 µg/ml Trypsin Gold (Promega)) for 30 min at 37°C shaking at 1,400 rpm in the dark ...
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bioRxiv - Biochemistry 2023Quote: ... before being washed and resuspended in 2 M urea/PBS and trypsinized overnight with 0.5 μg/μL sequencing-grade trypsin (Promega). Tryptic peptides were eluted ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were further diluted with 50 mM Tris pH 7.9 pH 8.0 to a final urea concentration of 2 M and proteins were digested with 5 µg trypsin (Promega) (1/100 ...
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bioRxiv - Neuroscience 2023Quote: ... Then the beads were incubated with 80 μL of 2 M urea in 50 mM Tris-HCL containing 1 mM DTT and 0.4 μg trypsin (Promega) at 25 °C for 1 hour while shaking at 1,000 rpm ...
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bioRxiv - Cell Biology 2023Quote: ... urea was diluted to 1 M and proteins digested overnight with modified sequencing grade trypsin (Promega, Madison, WI, USA) at a 1:50 enzyme:protein ratio ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were then diluted to 1 M urea and subjected to digestion with trypsin (Promega, 1:50 w/w) overnight at 37⁰C ...
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bioRxiv - Molecular Biology 2024Quote: ... pH 8.0 to an urea concentration of 4 M and proteins were digested with 40 µg endopeptidase LysC (Promega) (1/250 ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were further diluted with 20 mM HEPES pH 8.0 to a urea concentration of 2 M and proteins were digested with 50 µg trypsin (Promega) (1/200 ...
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bioRxiv - Neuroscience 2023Quote: ... extracellular and intracellular assays were performed to determine levels of HiBiT-tagged F3 using either the Nano-Glo HiBiT Extracellular Detection System or the Nano-Glo HiBiT Lytic Detection System (Promega). For the extracellular HiBiT protocol ...
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bioRxiv - Biochemistry 2020Quote: ... on a GloMax-discover multimode detection system (Promega) following the manufacturer’s instructions.
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bioRxiv - Genomics 2022Quote: ... the Nano-Glo HiBiT Lytic Detection System (Promega) was used for the quantification of the HiBiT tags ...
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bioRxiv - Immunology 2020Quote: ... and a GloMax-Multi Detection System (Promega, USA).
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bioRxiv - Pathology 2020Quote: ... and GloMax-Multi Detection System Photometer (Promega, USA).
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bioRxiv - Cell Biology 2019Quote: ... 2° antibodies α- mouse (HRP detection, Promega, W4021). The mean intensity quantification of 4 independent experiments is shown in Figure 4 – supplement 1B ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by luminescence detection using GloMax Explorer (Promega). The bioluminescence readings were averaged amongst the n=3 technical replicates for each construct and ratio ...
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bioRxiv - Cancer Biology 2024Quote: The NanoBRET™ Nano-Glo detection System (Promega) was used according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... which was detected with luciferase detection reagent (Promega). Luminescence measurements were done using a LUMIstar OMEGA plate reader (BMG ...
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bioRxiv - Systems Biology 2023Quote: ... 10 µl of MTase-Glo Detection Solution (Promega) was added ...
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bioRxiv - Cell Biology 2023Quote: ... we added 10 μl of detection reagent (Promega) to the system ...
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bioRxiv - Cell Biology 2023Quote: ... 100 µL Luciferin Detection Reagent (Promega Cat #TM344) was added to all wells ...
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bioRxiv - Molecular Biology 2023Quote: ... then 25 μl MTa-Glo Detection Solution (Promega) was added and incubated for a further 30 min at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... Plates containing the different transfected cells were incubated for 24 hours at 37°C before the luciferase activity was measured using the Dual-Glo luciferase detection kit (Promega: E2920).
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bioRxiv - Physiology 2021Quote: ... Extracts were used for quantification of luciferase as proxy for ATP content using the ENLITEN® ATP Assay System Bioluminescence Detection Kit (Promega) according to the manufacturer instructions ...
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Safety and efficacy of C9ORF72-repeat RNA nuclear export inhibition in amyotrophic lateral sclerosisbioRxiv - Systems Biology 2021Quote: ... Total Drosophila RNA was extracted from Drosophila heads which had been ground to a powder under liquid nitrogen before addition of 250 µl reporter lysis buffer (Promega) and addition of 750 µl PureZOL™ to the frozen lysates to extract the RNA ...
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bioRxiv - Biochemistry 2022Quote: ... The 90% buffer was removed and protoplasts were flash-frozen in liquid nitrogen to quench the protein degradation process followed by luciferase activity assay (E1500, Promega) to determine the relative amount of RAP2.12(1-28)-luciferase chimera protein remaining at various time points ...
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bioRxiv - Plant Biology 2022Quote: ... All samples were ground in liquid nitrogen and RNA was extracted using RNA isolation protocol for non-fiberous tissue by the RNA Tissue Miniprep System (Promega). cDNA synthesis was done using 1µg of total RNA with the qScriptTM cDNA Supermix kit (Quanta BioSciences) ...
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bioRxiv - Molecular Biology 2021Quote: ... FASP was carried out in two 10 kDa MWCO filters with a 50 mM iodoacetamide alkylation step and proteins were digested in 2M urea with 2% wt/wt Lys-C (Wako) for 6 h and 2% modified trypsin (Promega) for 12 h at 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... Twenty microliters of HPLC-grade water were added to reduce urea concentration and trypsin digestion was performed using a 1:20 (enzyme:protein) ratio of sequencing grade modified trypsin (Promega, V5111) and 18 h incubation at 37 °C on a ThermoMixer (Eppendorf ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein samples were re-solubilized in 6 M urea buffer followed by reduction and alkylation before digestion with trypsin (Promega) at 37°C for 18 hr ...
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bioRxiv - Biophysics 2020Quote: ... for 2 hours at 37° C and subsequently diluting to 1 M urea with 50 mM NH4HCO3 and finally adding 2% (w/w) trypsin (Promega) for 14 hours at 37° C ...
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bioRxiv - Cell Biology 2020Quote: ... proteins were diluted with 50 mM NH4HCO3 to a final concentration of 2M urea and digested with trypsin (Promega, UK), at an enzyme-to-substrate ratio of 1:20 ...
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bioRxiv - Genomics 2022Quote: ... samples were diluted 3 times with 50mM Tris buffer (down to 2M final urea concentration) and digested overnight at 37°C using a Trypsin/Lys-C mixture (Promega) at a ratio 1:100 w/w ...
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bioRxiv - Biochemistry 2022Quote: ... before being washed and resuspended in 2 M urea/PBS and trypsinized overnight with 0.5 μg /μL sequencing grade trypsin (Promega, V5111). Tryptic peptides were eluted off ...
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bioRxiv - Biochemistry 2022Quote: ... The samples were then diluted to 6 M urea with 50 mM Tris-HCl pH 8 and Trypsin/Lys-C mix (Promega) was added to an enzyme:substrate ratio of 1:25 (w/w ...
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bioRxiv - Cell Biology 2022Quote: ... The bound proteins were digested by resuspending the beads in 200 μL freshly prepared digestion buffer (2M Urea, 100 mM Tris-HCl pH 8, 100 mM DTT) with 0.5 ug trypsin (Promega, V5111) and overnight incubation at room temperature while shaking ...
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bioRxiv - Biochemistry 2022Quote: ... The samples were then diluted to 4 M urea with 50 mM Tris-HCl pH 8 and Trypsin/Lys-C mix (Promega) was added to an enzyme:substrate ratio of 1:250 (w/w ...
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bioRxiv - Molecular Biology 2020Quote: ... 20μg of total cell extracts each from heavy and light SILAC labels were combined and diluted to a final urea concentration of 1M and then digested overnight with LysC enzyme (Promega) at a 1:100 (enzyme:protein ...
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bioRxiv - Cell Biology 2019Quote: ... The proteins were digested in less than 4 M urea by endoproteinase LysC (Wako Chemicals) followed by digestion with Trypsin Gold (Promega) in less than 2 M urea ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were diluted to a concentration of urea of 2 mol L-1 and digested overnight with the addition of 2 µl sequencing-grade trypsin (Promega). After digestion ...
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bioRxiv - Cell Biology 2020Quote: ... The reduced and alkylated protein lysates were diluted using 50 mM Tris-Cl to obtain a final concentration of 2 M urea in solution followed by overnight digestion with trypsin (1:1000)(Promega). Peptides were then desalted using c-18 Sep-Pack columns (Waters) ...